We narrowed to 4,850 results for: U6...

1. pGL3-U6-sgRNA-PGK- mRFP-T2A-PuroR

   Plasmid

   #194925

   Purpose

   mRFP and T2A linker are inserted in between the hPGK promoter and the puromycin resistance gene (PuroR) on pGL3-U6-sgRNA-PGK-puromycin to allow simultaneous monitoring and enrichment of transfected ho

   Depositor

   Hao Xu

   Article

   Ayo et al J Genome Ed Regul. 2022;2:246146. doi: 1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   May 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. CAGGS-AsCpf1-2A-GFP-U6-INS-sgRNA

   Plasmid

   #159283

   Purpose

   A multicistronic vector with CAGGS promoter-driven AsCpf1 and U6 promoter-driven single guide RNA (sgRNA) targeting the human INS locus (sgINS).

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Rep Med. 2020 Sep 22;1(6):100097. d

   Inserts

   Human INS sgRNA
   Human INS guide RNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Sept. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-phsyn-FLEX-KASH-EGFP-U6-shRNA

   Plasmid

   #129708

   Purpose

   Express cre-dependent KASH-EGFP and scramble shRNA

   Depositor

   Yi Zhang

   Article

   Tuesta et al Nat Commun. 2019 Jun 7;10(1):2508. doi:

   Insert

   KASH-EGFP
   

   Use

   AAV

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. p1192-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIC3Nme_FLAG_NLS

   Plasmid

   #129530

   Purpose

   Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIC3Nme

   Depositor

   Erik Sontheimer

   Article

   Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

   Inserts

   codon-optimized AcrIIC3Nme
   sgRNA_Rosa26
   

   Use

   AAV

   Tags

   FLAG/NLS

   Expression

   Mutation

   Promoter

   U6 and cytomegalovirus-enhancer chicken β-actin p…

   Available since

   Oct. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. PiggyBac EF1a-Tet-on 3G-Puro U6 BbsI

   Plasmid

   #159756

   Purpose

   PiggybacTransposon-based expression control of Tet-on 3G, Puro and Cas13d U6 gRNA backbone

   Depositor

   Xiaoping Bao

   Article

   Chang et al Biomaterials. 2022 May 6;285:121569. doi

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-RSV-GFP-U6-gRNA scaffold (SpyCas9)

   Plasmid

   #113039

   Purpose

   AAV vector; encodes GFP as well as a U6-driven gRNA scaffold (SpyCas9)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   2x BbsI sites - SpCas9 scaffold, co-expressed GFP (transfection marker)
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-U6-sgCTG-CAG-Cas9D10A nickase-Blast

   Plasmid

   #216733

   Purpose

   Expresses SpCas9-D10A nickase under a CAG promoter together with a blasticidin resistance gene, along with a U6 promoter driving the sgCTG (target sequence: (CUG)6). Suitable for lentivirus packaging.

   Depositor

   Vincent Dion

   Article

   Alvaro et al bioRxiv

   Insert

   Cas9-D10A nickase and sgCTG
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A

   Promoter

   U6 and CAG

   Available since

   April 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pKSB-618 U6-sgRNA2/3 GGAA AACA

   Plasmid

   #173674

   Purpose

   To clone and express a gRNA in Anopheles

   Depositor

   Eric Marois

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-U6-PALMDgRNA1+2-cTnT-Cre -mi122TS

   Plasmid

   #216830

   Purpose

   cardiomyocytes-specific plasmid, expressing gRNAs targeting Palmd exon7, containing a mi122TS sequence to decrease off-target effects in the liver.

   Depositor

   Yuxuan Guo

   Article

   Guo et al Acta Pharmacol Sin. 2024 Jul 23. doi: 10

   Insert

   PALMDgRNA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR221-H1-sgGFP1-U6-sgGFP2-7SK-sgGFP3

    Plasmid

    #87906

    Purpose

    sgRNA targeting GFP

    Depositor

    Nicole Déglon

    Article

    Merienne et al Cell Rep. 2017 Sep 19;20(12):2980-2991.

    Insert

    sgGFP1, sgGFP2, sgGFP3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1, U6, 7SK

    Available since

    Sept. 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. U6-Slc1a3 sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194283

    Purpose

    EF1a-dCas9-KRAB-GFP with Slc1a3 sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. p1194-scAAV-U6-sgRNA_Nme2Cas9_Rosa26-CB-PI-AcrIIA4_FLAG_NLS

    Plasmid

    #129532

    Purpose

    Self-complementary AAV vector expressing Nme2Cas9 sgRNA targeting Rosa26 and AcrIIA4

    Depositor

    Erik Sontheimer

    Article

    Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

    Inserts

    codon-optimized AcrIIA4
    sgRNA_Rosa26
    

    Use

    AAV

    Tags

    FLAG/NLS

    Expression

    Mutation

    Promoter

    U6 and cytomegalovirus-enhancer chicken β-actin p…

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEJS2604_AAV-Nme2-ABE8e-i1(V106W)-U6-Rosa26

    Plasmid

    #201686

    Purpose

    All-in-one AAV plasmid expressing Nme2-ABE8e-i1 (V106W) and U6 driven sgRNA targeting the mouse Rosa26 gene

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al Nat Commun. 2024 Feb 17;15(1):1458. doi:

    Insert

    Nme2-ABE8e-i1 (V106W)
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U1a

    Available since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLKO.1-puro U6 sgRNA SOX17 -177

    Plasmid

    #50925

    Purpose

    U6 driven sgRNA targeting Sox17 -177 bp from TSS

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    Sox17 -177 sgRNA (SOX17 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. XLone-Puro Cas13d-eGFP U6 SOX17 g2

    Plasmid

    #155188

    Purpose

    PiggybacTransposon-based tunable and temporal expression control of Cas13d- eGFP and SOX17 gRNA2

    Depositor

    Xiaoping Bao

    Article

    Chang et al Biomaterials. 2022 May 6;285:121569. doi

    Insert

    Cas13d SOX17 gRNA2
    

    Use

    Piggybac transposon

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-U6-sgRNA(OXTR.1)-CMV-eGFP

    Plasmid

    #194015

    Purpose

    Expresses a gRNA that targets OXTR coding sequence in multiple (rodent) species and eGFP

    Depositor

    Larry Young

    Article

    Boender et al Sci Adv. 2023 Jun 2;9(22):eadf4950. doi:

    Inserts

    sgRNA(Oxtr.1)
    eGFP
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and u6

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

    Plasmid

    #114087

    Purpose

    U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for FnCas12a crRNAs
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSIN-U6-tracer_C9_1_-EF1a-Thy1.1-P2A-Neo

    Plasmid

    #191397

    Purpose

    sgRNA targeting alpha-satellites on human chromosome 9

    Depositor

    Albert Jeltsch

    Article

    Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

    Insert

    sgRNA targeting alpha satellites on human chromosome 9
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. BC1441-mouse U6-3xsgRNAs (TS5, TS6, TS7)

    Plasmid

    #164040

    Purpose

    Expression of three sgRNAs (sgTS5, sgTS6, sgTS7) for targeting to CRISPR-Tag_V2

    Depositor

    Baohui Chen

    Article

    Chen et al Nat Commun. 2018 Nov 29;9(1):5065. doi:

    Insert

    sgTS5-sgTS6-sgTS7
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    mouse U6

    Available since

    Jan. 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ1869-1 pHR: U6-SpsgSV40 CMV-mCherry

    Plasmid

    #84249

    Purpose

    Expresses Sp sgSV40 gRNA with a mCherry fluorescent marker

    Depositor

    Stanley Qi

    Article

    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n

    Insert

    Sp sgSV40
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge