164,577 results

1. pBS hTSP-5

   Plasmid

   #13002

   Depositor

   Jack Lawler

   Article

   Newton et al Genomics. 1994 Dec . 24(3):435-9.

   Insert

   COMP (COMP Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Sept. 28, 2006

   Availability

   Academic Institutions and Nonprofits only

2. AAV-CMV-GFP

   Plasmid

   #67634

   Purpose

   AAV vector expressing GFP

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   GFP
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. mCherry-Ezrin-N-14

   Plasmid

   #55043

   Purpose

   Localization: Focal Adhesions/Membrane, Excitation: 587, Emission: 610

   Depositor

   Michael Davidson

   Insert

   Ezrin (EZR Human)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTP1172

   Plasmid

   #104161

   Purpose

   Bacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (3x Cysteines)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-mouse kappa chain specific nanobody TP1170 (3xCysteines)
   

   Use

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET30-2-GPD1

   Plasmid

   #83911

   Purpose

   stable overexpression

   Depositor

   David Sabatini

   Article

   Pacold et al Nat Chem Biol. 2016 Jun;12(6):452-8. doi

   Insert

   glycerol-3-phosphate dehydrogenase 1 (GPD1 Human)
   

   Use

   Tags

   6x His tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. GFP-RAPH1ΔTBS

   Plasmid

   #194859

   Purpose

   Express eGFP-RAPH1 lacking the TBS domain (RAPH1 aa 2-92 deleted) in mammalian cells.

   Depositor

   Guillaume Jacquemet

   Article

   Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

   Insert

   RAPH1 (RAPH1 Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   RAPH1 with amino acids 2-92 deleted

   Promoter

   CMV

   Available Since

   March 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pEff

   Plasmid

   #97423

   Purpose

   Transient expression of recombinant proteins in plants. The plasmid contains gfp, which is replaced by the gene of interest.

   Depositor

   Nikolai Ravin

   Article

   Mardanova et al Front Plant Sci. 2017 Feb 28;8:247. doi:

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   July 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. FLAG-mBAF45a IRES GFP

   Plasmid

   #17880

   Depositor

   Jerry Crabtree

   Article

   Lessard et al Neuron. 2007 Jul 19. 55(2):201-15.

   Insert

   BAF45a (Phf10 Mouse)
   

   Use

   Tags

   FLAG and IRES EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 13, 2008

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLenti-CMV-FlaviA-mNeptune-puro

   Plasmid

   #140091

   Purpose

   Fluorescence activatable reporter of flavivirus NS2B-NS3 protease activity

   Depositor

   Jorge Luis Arias-Arias

   Article

   Arias-Arias et al J Biol Chem. 2020 Jan 9. pii: RA119.0113

   Insert

   FlaviA-mNeptune
   

   Use

   Lentiviral; High expression

   Tags

   mNeptune

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FU-tetO-Gateway

    Plasmid

    #43914

    Depositor

    John Gearhart

    Article

    Addis et al PLoS One. 2011;6(12):e28719. doi: 10.137

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector, doxycycli…

    Tags

    6xHis and V5

    Expression

    Mammalian

    Mutation

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pIRES-HA-14-3-3 ϵ/myc-14-3-3ζ

    Plasmid

    #48795

    Purpose

    expresses human HA-14-3-3 ϵ (epsilon) and myc-14-3-3ζ (zeta) in mammalian cells

    Depositor

    Huda Zoghbi

    Article

    Chen et al Cell. 2003 May 16;113(4):457-68.

    Inserts

    YWHAE (YWHAE Human)
    YWHAZ (YWHAZ Human)
    

    Use

    Tags

    HA and myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and CMV (IRES)

    Available Since

    May 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-sfGFP 150TAG

    Plasmid

    #85493

    Purpose

    pET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150

    Depositor

    Ryan Mehl

    Article

    Peeler et al Methods Mol Biol. 2012;794:125-34. doi:

    Insert

    superfolder GFP 150TAG
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    150 TAG (amino acid 150 mutated to stop codon)

    Promoter

    T7

    Available Since

    Jan. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. GPR141-Tango

    Plasmid

    #66318

    Purpose

    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

    Depositor

    Bryan Roth

    Article

    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

    Insert

    GPR141 (GPR141 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pSD5

    Plasmid

    #197289

    Purpose

    Contains a seamless deletion cassette for the replacement of ORF2230 in D. radiodurans

    Depositor

    Bogumil Karas

    Article

    Brumwell et al Biodes Res. 2023 Mar 15;5:0009. doi: 10.

    Insert

    NptII
    

    Use

    Synthetic Biology; Bacterial and yeast cloning

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGEX:PGRMC1

    Plasmid

    #136062

    Purpose

    Bacterial expression of pGEX:PGRMC1 to perform a GST-pulldown assay and identify new proteins that interact with GST-PGRMC1 in the human endometrial stroll cells.

    Depositor

    Francisco Domínguez Hernández

    Article

    Salsano et al Fertil Steril. 2020 May;113(5):1050-1066

    Insert

    Progesterone receptor membrane component 1 (PGRMC1 Human)
    

    Use

    Tags

    GST-PGRMC1

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. EBFP2-VASP-5

    Plasmid

    #55251

    Purpose

    Localization: Actin/Focal Adhesions, Excitation: 383, Emission: 448

    Depositor

    Michael Davidson

    Insert

    VASP (Vasp Mouse)
    

    Use

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    A209T in VASP

    Promoter

    CMV

    Available Since

    Oct. 22, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pKmK.T4

    Plasmid

    #125056

    Purpose

    Storage plasmid for K.marxianus terminator; type 4 part

    Depositor

    John Morrissey

    Article

    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97

    Insert

    PDC1 terminator
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAG148 FRB-NFAST

    Plasmid

    #130812

    Purpose

    Expresses FRB-NFAST in mammalian cells

    Depositor

    Arnaud Gautier

    Article

    Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

    Insert

    FRB-NFAST
    

    Use

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-EF1a-IRES-Neo

    Plasmid

    #85139

    Purpose

    lentiviral backbone

    Depositor

    Tobias Meyer

    Article

    Hayer et al Nat Cell Biol. 2016 Dec;18(12):1311-1323

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Feb. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLVX-HA-UBE3B-IRES-Puro

    Plasmid

    #107345

    Purpose

    Expresses HA tagged UBE3B in mammalian cells.

    Depositor

    Robert Sobol

    Article

    Braganza et al J Biol Chem. 2016 Dec 21. pii: jbc.M116.

    Insert

    UBE3B (UBE3B Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 19, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge