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TALED_Left-ND1-1397C-AD

Plasmid

#183892

Purpose

Expresses TALED_Left-ND1-1397C-AD in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-PDZD8HA

Plasmid

#105005

Purpose

Mammalian Expression of PDZD8

Depositor

Franck Polleux

Article

Hirabayashi et al Science. 2017 Nov 3;358(6363):623-630. d

Insert

PDZD8 (Pdzd8 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV5B-TGFbeta receptor II wt

Plasmid

#11766

Depositor

Joan Massague, Jeff Wrana

Article

Wrana et al Cell. 1992 Dec 11. 71(6):1003-14.

Insert

TGFbeta receptor II (TGFBR2 Human)

Tags

HA

Expression

Mammalian

Available Since

May 12, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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CMV-Y-GECO1

Plasmid

#55766

Purpose

Encoded a yellow fluorescent calcium ion indicator with inverted fluorescence response for expression in mammalian cells

Depositor

Robert Campbell

Article

Zhao et al Integr Biol (Camb). 2014 May 19.

Insert

Y-GECO1.0

Tags

6x His tag

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pCMV-RosaL6 ELD mutations

Plasmid

#37198

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nucleic Acids Res. 2012 Apr 1;40(8):3741

Insert

FokI (fused to Rosa L6 ZFN)

Use

Zfn expression

Tags

HA, ROSA26 eZFNs L6, and SV40 NLS

Expression

Mammalian

Mutation

Q486E, I499L and N496D

Promoter

CMV

Available Since

July 19, 2012

Availability

Academic Institutions and Nonprofits only

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7TFC

Plasmid

#24307

Depositor

Roel Nusse

Article

Fuerer et al PLoS One. 2010 . 5(2):e9370.

Insert

7xTcf-FFluc // SV40-mCherry

Use

Lentiviral

Available Since

April 5, 2010

Availability

Academic Institutions and Nonprofits only

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pYJA5

Plasmid

#217778

Purpose

The empty vector for quadruple sgRNA cloning

Depositor

Adriano Aguzzi

Article

Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

Type

Empty backbone

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

Puromycin resistance gene, T2A, and TagBFP

Expression

Mammalian

Promoter

PGK, CMV, U6

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTMiniTrex-mCherry-Shark1

Plasmid

#233143

Purpose

Serves as a template plasmid for amplifying Shark1 (Tet-OFF) hammerhead aptazyme-3xTy-tagging cassette

Depositor

Ronald Etheridge

Article

Wiedeman et al Proc Natl Acad Sci U S A. 2025 Feb 25;12

Inserts

mCherry
Shark1

Use

Prokaryotic expression

Tags

3x Ty tag

Mutation

Codon optimized for Trypanosoma cruzi and changed…

Available Since

March 25, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

Plasmid

#126705

Purpose

donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

Depositor

Darrell Kotton

Article

Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

Insert

GFP

Use

CRISPR

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

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pMIAII-Cd4

Plasmid

#194401

Purpose

Retroviral expression of mouse CD4

Depositor

Michael Carroll

Article

Akama-Garren et al Sci Immunol. 2024 Feb 16;9(92):eadj7029.

Insert

Cd4 (Cd4 Mouse)

Use

Retroviral

Mutation

WT

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT_mEGFP-SNAP-tag2

Plasmid

#226513

Purpose

Mamalian cell expression of cytosolic mEGFP-SNAP-tag2

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

mEGFP-SNAP-tag2

Expression

Mammalian

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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V5-GFP-TurboID-KDEL_pLX208

Plasmid

#202020

Purpose

expresses GFP-TurboID in the mammalian ER lumen, lentiviral vector

Depositor

Alice Ting

Article

Qin et al Cell. 2023 Jun 23:S0092-8674(23)00596-2.

Insert

V5-TurboID-KDEL

Use

Lentiviral

Tags

GFP and V5

Expression

Mammalian

Promoter

CMV

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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AAV-P(Cry1)-DIO-intron336-Venus-NLS-D2

Plasmid

#110055

Purpose

Cry1 transcription fluorescence reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Cry1 promoter and Venus (Cry1 Mouse)

Use

AAV and Cre/Lox

Tags

NLS-D2

Expression

Mammalian

Promoter

Cry1

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-DIO-PinkyCaMP

Plasmid

#232857

Purpose

An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under Syn promoter in a Cre-dependent manner (DIO, double floxed inverted open reading frame)

Depositor

Olivia Masseck

Article

Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

Insert

PinkyCaMP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp8

Plasmid

#11817

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 8 (CASP8 Human)

Expression

Mammalian

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pAM236 Ubc4

Plasmid

#226361

Purpose

Expression of yeast Ubc4

Depositor

Andreas Martin

Article

Bashore et al Nat Struct Mol Biol. 2015 Sep;22(9):712-

Insert

Ubc4 (UBC4 Budding Yeast)

Tags

His

Expression

Bacterial

Promoter

T7

Available Since

Nov. 13, 2024

Availability

Academic Institutions and Nonprofits only

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TALED_Right-ND1-1397N

Plasmid

#183898

Purpose

Expresses TALED_Right-ND1-1397N in mammalian cells

Depositor

Jin-Soo Kim

Article

Cho et al Cell. 2022 May 12;185(10):1764-1776.e12.

Insert

TALED for ND1 mutation

Expression

Mammalian

Promoter

pCMV

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b-His-RF2

Plasmid

#124130

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Release factor 2 (prfB Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP‐RNASEH2A

Plasmid

#108700

Purpose

For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

Depositor

Andrew Jackson, Martin Reijns

Article

Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

Insert

RNASEH2A (RNASEH2A Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAP2K5

Plasmid

#20514

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP2K5 (MAP2K5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only

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