Addgene

We narrowed to 21,419 results for: ESC;

Showing: 7771 - 7800 of 21419 results

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pQFT-lacZ

Plasmid

#208896

Purpose

pQFT with lacZ under control of PQJ

Depositor

Urs Jenal

Article

Klotz et al Appl Environ Microbiol. 2023 May 18:e002

Insert

lacZ

Use

Tags

Expression

Bacterial

Mutation

Contains two silent mutations and a missense muta…

Promoter

PQJ

Available since

Dec. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pET29b-ClbPpep-CHis

Plasmid

#48243

Purpose

First 375 amino acids of the peptidase, which contains the soluble periplasmic peptidase domain

Depositor

Emily Balskus

Article

Brotherton et al J Am Chem Soc. 2013 Mar 6;135(9):3359-62

Insert

ClbP-pep

Use

Tags

6-His

Expression

Bacterial

Mutation

First 375 amino acids of the peptidase, which con…

Promoter

T7

Available since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pEB1-mYPet

Plasmid

#103988

Purpose

Plasmid encoding mYPet

Depositor

Philippe Cluzel

Article

Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

Insert

mYPet

Use

Low copy

Tags

Expression

Bacterial

Mutation

Mutations relative to wild-type GFP (F46L, I47L, …

Promoter

proC

Available since

Dec. 14, 2017

Availability

Academic Institutions and Nonprofits only

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PEP114E

Plasmid

#97400

Purpose

binary construct of ER-targeted mCherry with sfGFP11 tag for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

mCherry-sfGFP11

Use

Tags

ER target

Expression

Plant

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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PEP119T

Plasmid

#98251

Purpose

Pseudomonas T3SS gateway vector with HA-sfGFP11-sfGFP11

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

Gateway cassettee-HA-sfGFP11-11

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pSB_init_Sb68

Plasmid

#159423

Purpose

Expression of the anti-TGP (thermostable GFP) synthetic nanobody Sb68

Depositor

Dianfan Li

Article

Cai et al Commun Biol. 2020 Dec 10;3(1):753. doi:

Insert

Sb68

Use

Tags

c-Myc and poly-His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pGK401red-rtetTAK8N, L131L

Plasmid

#165968

Purpose

Expresses rTetTA (K8N, L131L) mutant

Depositor

Jun Ishii

Article

Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

Insert

tetR

Use

Synthetic Biology

Tags

three copies of VP16 (VP48)

Expression

Yeast

Mutation

Promoter

Available since

March 29, 2021

Availability

Academic Institutions and Nonprofits only

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mCerulean-H2B-6

Plasmid

#55374

Purpose

Localization: Nucleus/Histones, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

H2B (H2BC11 Human)

Use

Tags

mCerulean

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pNB783

Plasmid

#60162

Purpose

Firefly luciferase (Promega) driven by RNR1 promoter.

Depositor

Nicolas Buchler

Article

Mazo-Vargas et al Mol Biol Cell. 2014 Sep 17. pii: mbc.E14

Insert

FLuc

Use

Tags

Expression

Yeast

Mutation

A4V & S504G (no functional effect)

Promoter

RNR1pr

Available since

March 25, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoPA-TagRFP-SpHis5

Plasmid

#44854

Purpose

Yeast tagging vector to create gene fusion with yeast optimized photoactivatable TagRFP with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoPA-TagRFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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PEP103E

Plasmid

#97389

Purpose

binary construct of plastid-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

Plastid target

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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PEP105E

Plasmid

#97391

Purpose

binary construct of peroxisome-targeted sfGFP1-10 for plant expression

Depositor

Savithramma Dinesh-Kumar

Article

Park et al Plant Cell. 2017 Jul;29(7):1571-1584. do

Insert

sfGFP1-10OPT

Use

Tags

Peroxisome target

Expression

Plant

Mutation

Promoter

Available since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJC20cdc8-cerulean3

Plasmid

#99358

Purpose

Bacterial expression of Cerulean3 fused to the 3' of cDNA of the fission yeast tropomyosin, cdc8.

Depositor

Dan Mulvihill

Article

Brooker et al FEBS Lett. 2016 Sep;590(18):3111-21. doi

Insert

cdc8 (cdc8 Fission Yeast)

Use

Tags

Cerulean3 and Gly3-His6

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

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pXcX-3.6TPH-GAL4p65

Plasmid

#32573

Depositor

Sergey Kasparov

Article

Benzekhroufa et al BMC Biotechnol. 2009 Mar 19;9:23.

Insert

Gal4p65

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

Promoter

3.6TPH

Available since

Jan. 3, 2012

Availability

Academic Institutions and Nonprofits only

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DmU6 3p3 GFP LgRNA

Plasmid

#182207

Purpose

For generation of plasmids for CRISPR-mediated 3p3 GFP knockin in Drosophila

Depositor

Craig Montell

Article

Chen et al Genetics. 2021 Nov 5;219(3). pii: 636806

Insert

3XP3-GFP

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

April 5, 2022

Availability

Academic Institutions and Nonprofits only

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pBSV2G_PflaB-mEGFPBb

Plasmid

#118234

Purpose

Gentamicin-resistant E.coli/B. burgdorferi shuttle vector; Encodes flagellin promoter driven mEGFP

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

flagellin promoter driven mEGFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 20, 2018

Availability

Academic Institutions and Nonprofits only

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IFP1.4-N1

Plasmid

#54783

Purpose

Localization: N1 Cloning Vector, Excitation: 684, Emission: 708

Depositor

Roger Tsien, Michael Davidson

Article

Shu et al Science. 2009 May 8;324(5928):804-7. doi

Type

Empty backbone

Use

Tags

IFP1.4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pAG372-mito-greenFAST

Plasmid

#160357

Purpose

Expresses mito-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

mito-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pTYF-EF1a-DLDH-IRES-EGFP

Plasmid

#178310

Purpose

Express the bacterial D-lactate dehydrogenase (DLDH) enzyme in mammalian cells

Depositor

Sergey Kasparov

Article

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

D-lactate dehydrogenase

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pAG551-MAP4-greenFAST

Plasmid

#160366

Purpose

Expresses MAP4-greenFAST in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Chem Biol. 2020 Aug 10. pii: 10.1038

Insert

MAP4-greenFAST

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yomKeima-SpHis5

Plasmid

#44847

Purpose

Yeast tagging vector to create gene fusion with yeast optimized mKeima with SpHis5 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yomKeima

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 10, 2013

Availability

Academic Institutions and Nonprofits only

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mRuby-H2B-6

Plasmid

#55866

Purpose

Localization: Nucleus/Histones, Excitation: 558, Emission: 605

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

H2B (H2BC11 Human)

Use

Tags

mRuby

Expression

Mammalian

Mutation

D26G and V119I in H2B

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-Tubulin-C-18

Plasmid

#57432

Purpose

Localization: Microtubules, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

Tubulin (TUBA1B Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pAS1_4xh7SKPylT_EF1_GFP*_Intein_DD

Plasmid

#162812

Purpose

STELLA plasmid for amber suppression of C-terminally labelled GFP

Depositor

Simon Elsaesser

Article

Lafranchi et al J Am Chem Soc. 2020 Nov 25;142(47):20080

Insert

GFP(C-TAG)-Intein-DD

Use

Piggybac

Tags

Expression

Mammalian

Mutation

GFP(Opal247,Amber)

Promoter

EF1alpha

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-CaURA3

Plasmid

#44886

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with CaUra3 selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pJRK260

Plasmid

#173753

Purpose

CeLINC plasmid

Depositor

Mike Boxem

Article

Kroll et al Genetics. 2021 Dec 10;219(4). pii: 63804

Insert

Prps-0::CRY2(olig)::VHH(GFP)::T2A::CIBN-3xFLAG::MP::unc-54 3' UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Prps-0

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pJB028

Plasmid

#65469

Purpose

pTrc plasmid expressing p14*-TEV

Depositor

Keith Tyo

Article

Sekar et al PLoS One. 2016 Feb 22;11(2):e0149746. do

Insert

p14*-TEV (TEVgp1 )

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

trc

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB401NL3F10H

Plasmid

#141285

Purpose

No promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, KmR for plant)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Type

Empty backbone

Use

Luciferase; Gateway

Tags

FLAG (x3), HIS (x10), and NLUC

Expression

Plant

Mutation

Promoter

Available since

Feb. 12, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoClover-Kan

Plasmid

#44952

Purpose

Yeast tagging vector to create gene fusion with yeast optimized Clover with Kan selection

Depositor

Kurt Thorn, Wendell Lim

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoClover

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

May 14, 2013

Availability

Academic Institutions and Nonprofits only

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pQUAST-syb-spCFP1-10

Plasmid

#73699

Purpose

Neuronal-synaptobrevin (syb):spCFP1–10 chimera for synaptic GRASP

Depositor

Marco Gallio

Article

Macpherson et al Nat Commun. 2015 Dec 4;6:10024. doi: 10.

Insert

Neuronal-synaptobrevin:spCFP1–10 chimera

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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