We narrowed to 9,825 results for: 158

1. pEGFP-mKOR

   Plasmid

   #179285

   Purpose

   Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

   Depositor

   Christian Gruber

   Article

   Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

   Insert

   kappa opioid receptor (Oprk1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Nsp10-mCherry

   Plasmid

   #165135

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp10 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLX209-neo-HA-Ub-T2A-Tet3G

   Plasmid

   #202436

   Purpose

   Bicistronic expression of HA-tagged Ubiquitin (HA-Ubiquitin) and Tet-On 3G transactivator (Tet3G)

   Depositor

   William Hahn

   Article

   Cervia et al Cancer Discov. 2023 Mar 1;13(3):766-795.

   Insert

   Ubiquitin
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1alpha

   Available since

   June 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Nsp4-mCherry

   Plasmid

   #165132

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp4 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Blast-sgAMPKa1

   Plasmid

   #138704

   Purpose

   Expresses a human AMPKa1-targeting sgRNA and Cas9

   Depositor

   Reuben Shaw

   Article

   Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

   Insert

   sgAMPKa1 human (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-Nsp15

   Plasmid

   #165120

   Purpose

   mammalian expression and localization

   Depositor

   Bruno Antonny

   Article

   Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

   Insert

   Nsp15 (ORF1ab codon optimized SARS-COV-2)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiCRISPR v2 puro hGSDME gRNA1

   Plasmid

   #223519

   Purpose

   knocking out hGSDME in human cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (GSDME Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MmEos3.2

   Plasmid

   #203754

   Purpose

   Encodes the membrane anchor of Src including the first 15 resideues of the N terminus, with 1 myristoylation and short polybasic sequence, with mEos3.2 fused to the C terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   Src15 n-term (SRC Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLVX-puro-mGSDME D270A

   Plasmid

   #223518

   Purpose

   Expresses mGSDME D270A in mammalian cells

   Depositor

   Judy Lieberman

   Article

   Zhang et al Nature. 2020 Mar;579(7799):415-420. doi:

   Insert

   GSDME (Gsdme Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D270A

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nsp6-mCherry

    Plasmid

    #165133

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp6 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCLXSN-c-JUN

    Plasmid

    #102758

    Purpose

    Expression of c-Jun

    Depositor

    Jin Chen

    Article

    Song et al Cancer Res. 2014 May 1;74(9):2444-54. do

    Insert

    c-Jun (JUN Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pINDUCER dCas9-TET1CDMut

    Plasmid

    #101920

    Purpose

    The dCas9-TET1CDMut fusion protein is cloned into the pINDUCER lentiviral backbone (Addgene plasmid# 46948). The TET1 catalytic domain is mutated to abolish catalytic function.

    Depositor

    Danwei Huangfu

    Article

    Verma et al Nat Genet. 2018 Jan;50(1):83-95. doi: 10

    Insert

    dCas9-TET1CD Mut
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Mutated TET1 catalytic domain

    Promoter

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCDNA3.1_Zeo_TetO_FLAG-AT1R

    Plasmid

    #222856

    Purpose

    Mammalian expression plasmid for FLAG-tagged AT1R

    Depositor

    Andrew Kruse

    Article

    Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

    Insert

    AT1R (AGTR1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Nsp13-mCherry

    Plasmid

    #165136

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp13 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pPBbsr2-Gnaq

    Plasmid

    #170298

    Purpose

    Encoding mouse Gnaq

    Depositor

    Michiyuki Matsuda

    Article

    Konishi et al Cancer Res. 2021 May 25. pii: 0008-5472.

    Insert

    A recombinant mouse Gnaq (Gnaq Mouse)
    

    Use

    Tags

    N/A

    Expression

    Mammalian

    Mutation

    Asilent mutation was introduced into the sgRNA-ta…

    Promoter

    Available since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. K-to-R mutant NPRL2(1-380) in pLJM60

    Plasmid

    #184562

    Purpose

    CMV-driven expression of an untagged mutant NPRL2 (core subunit of GATOR1) — native sequence for stable expression in mammalian cells. All lysine residues were mutated to arginines.

    Depositor

    Kacper Rogala

    Article

    Valenstein et al Nature. 2022 Jul;607(7919):610-616. doi:

    Insert

    NPRL2 (NPRL2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    All lysines were mutated to arginines.

    Promoter

    CMV

    Available since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pH-nCas9-PPE

    Plasmid

    #140447

    Purpose

    For plant prime editing in rice plants or monocotyledons protoplasts

    Depositor

    Caixia Gao

    Article

    Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

    Insert

    nCas9(H840A)-M-MLV
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    H840A for Cas9; D200N, T306K, W313F, T330P and L…

    Promoter

    maize Ubiquitin-1, OsU3

    Available since

    May 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOT_1 - lenti-EFS-LTBR-2A-puro

    Plasmid

    #181970

    Purpose

    Expresses human LTBR linked to puromycin resistance via P2A for lentiviral delivery.

    Depositor

    Neville Sanjana

    Article

    Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

    Insert

    LTBR (LTBR Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRecLV105

    Plasmid

    #107238

    Purpose

    A lentiviral vector for expression in mammalian cells

    Depositor

    Alex Kentsis

    Article

    Brown et al Cancer Discov. 2018 Apr;8(4):478-497. do

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTRIPZ-TIR1-HA

    Plasmid

    #172610

    Purpose

    Lentiviral vector for the Tet-inducible expression of HA-tagged TIR1 and a constitutive puromycin resistance marker in mammalian cells

    Depositor

    Michele Pagano

    Article

    Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

    Insert

    TIR1
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Tet/ON Minimal CMV promoter

    Available since

    Aug. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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21. pAAV-Syn-SomArchon-P2A-CoChR-Kv2.1

    Plasmid

    #126945

    Purpose

    Expression of SomArchon-P2A-CoChR-Kv2.1 under Syn promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-P2A-CoChR-Kv2.1
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AAV-Syn-FLEX-rc [SomArchon-GFP]

    Plasmid

    #153533

    Purpose

    AAV-mediated expression of SomArchon-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner.

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon-GFP
    

    Use

    AAV

    Tags

    ER2, GFP, and KGC

    Expression

    Mammalian

    Mutation

    Promoter

    Syn

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pAAV-GFAP-eGFP-shLacZ

    Plasmid

    #223222

    Purpose

    mir30 based shRNA strategy, control shRNA

    Depositor

    Li-Huei Tsai

    Article

    Murdock et al Nature. 2024 Mar;627(8002):149-156. doi:

    Insert

    shLacZ
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLentiCMV-Blast mLST8-Flag

    Plasmid

    #124915

    Purpose

    For stable expression sgRNA resistant Flag tagging mLST8_

    Depositor

    Jin Chen

    Article

    Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

    Insert

    MLST8 (MLST8 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Insert ORF was sirently mutated to be resistent t…

    Promoter

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-sgStk11#2/Cre

    Plasmid

    #173653

    Purpose

    Expresses a Stk11-targeting gRNA and Cre-recombinase

    Depositor

    Charles Swanton

    Article

    Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

    Insert

    gRNA targeting Stk11 (Stk11 Mouse)
    

    Use

    CRISPR, Cre/Lox, and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCR8/GW_TOPO_Cdk12_Nterm_FlagHa

    Plasmid

    #127177

    Purpose

    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into Gateway entry vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Entry vector with transgene for gateway cloning

    Tags

    Flag- HA- Tandem Epitopes

    Expression

    Mutation

    Promoter

    None

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pLenti-Puro-sgAMPKa1

    Plasmid

    #138684

    Purpose

    Expresses a human AMPKa1-targeting sgRNA and Cas9

    Depositor

    Reuben Shaw

    Article

    Hollstein et al Cancer Discov. 2019 Nov;9(11):1606-1627.

    Insert

    sgAMPKa1 human (PRKAA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Feb. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCDF-Az

    Plasmid

    #197101

    Purpose

    To express the azidophenylalanine-specific variant of Methanocaldococcus jannaschii TyrRS and its cognate amber suppressor tRNA and allow UAG codon to be translated into azidophenylalanine

    Depositor

    Kensaku Sakamoto

    Article

    Yamaguchi et al Bioconjug Chem. 2016 Jan 20;27(1):198-20

    Insert

    TyrRS variant, amber suppressor tRNA, kanamycin resistance gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Thr32, Asn107, Pro158, Leu159, Gln162, Arg286 (Ty…

    Promoter

    Available since

    June 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Nsp7-EGFP

    Plasmid

    #165111

    Purpose

    mammalian expression and localization

    Depositor

    Bruno Antonny

    Article

    Miserey-Lenkei et al Biol Cell. 2021 Jul;113(7):311-328. doi:

    Insert

    Nsp7 (ORF1ab codon optimized SARS-COV-2)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. PB-Neo-TetO-Cdk12_Untagged

    Plasmid

    #127178

    Purpose

    Untagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector

    Depositor

    Phil Sharp

    Article

    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:

    Insert

    Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    

    Use

    Piggybac transposase vector

    Tags

    None

    Expression

    Mammalian

    Mutation

    Promoter

    TetO Promoter

    Available since

    July 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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