We narrowed to 5,415 results for: PEP;

1. ABL1-C-HiBiT

   Plasmid

   #234568

   Purpose

   Bacterially expressed ABL1-C-HiBiT for Ni-NTA purification

   Depositor

   James Inglese

   Article

   Ciulla et al Nat Commun. 2025 May 10;16(1):4342. doi:

   Insert

   Abelson Kinase Domain (ABL1 Human)
   

   Use

   Tags

   HiBiT Tag-thrombin site-10xHis and T7 gene 10 lea…

   Expression

   Bacterial

   Mutation

   Codon optimized for bacterial expression

   Promoter

   T7

   Available since

   May 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAVS1-TRE3G-SOX2-3xHA-P2A-tagBFP

   Plasmid

   #163701

   Purpose

   Dox-inducible SOX2-3xHA HDR knock-in cassette into the AAVS1 locus with a tagBFP fluorescent marker linked by a self-cleaving P2A peptide.

   Depositor

   Jennifer Mitchell

   Article

   Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

   Inserts

   SOX2-3xHA-P2A-tagBFP (SOX2 Human)
   rtTA
   T2-sgRNA
   

   Use

   AAV and CRISPR

   Tags

   3x-HA and P2A-tagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and TRE3G

   Available since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. ρ1-EM GABAA receptor in pEZT-BM

   Plasmid

   #213072

   Purpose

   Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD

   Depositor

   Erik Lindahl

   Article

   Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.

   Insert

   Human GABAa rho1 receptor (GABRR1 Human)
   

   Use

   Bacmam, baculovirus, oocyte expression

   Tags

   Twin-Strep tag and superfolderGFP

   Expression

   Mammalian

   Mutation

   Encodes residues S58–L383 and D451–S479, separate…

   Promoter

   CMV and T7

   Available since

   Feb. 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLKO shLuc/FLAG Metap2

   Plasmid

   #209316

   Purpose

   Lentiviral construct expressing FLAG Metap2 and shRNA against Luciferase (control shRNA)

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 human, Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Dec. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA4/TO-bio-myc-cCE

   Plasmid

   #112711

   Purpose

   For tetracycline-inducible expression of bio-myc-NES-mCE ΔNLS ("bio-myc-cCE") in mammalian cells

   Depositor

   Daniel Schoenberg

   Article

   Trotman et al J Biol Chem. 2018 Oct 26;293(43):16596-1

   Insert

   Rngtt (Rngtt Mouse)
   

   Use

   Tags

   HIV Rev nuclear localization signal (NES), Stop c…

   Expression

   Mammalian

   Mutation

   Silent mutation at Arg 132 (CGT to CGC), deleted …

   Promoter

   CMV with Tet repressor binding sites

   Available since

   Sept. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. R620-M18-303: CMV51p> SARS-CoV-2 S-RBD(319-541)-His6 K417T/E484K/N501Y

   Plasmid

   #170202

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. R620-M19-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-529)-3C-His8-SBP K417T/E484K/N501Y

   Plasmid

   #170203

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), N501Y mutant from B.1.1.28 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   3C-His8-SBP

   Expression

   Mammalian

   Mutation

   K417T, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. R620-M05-303: CMV51p> TPAss-SARS-CoV-2 S-RBD(319-541)-His6 K417N/E484K/N501Y

   Plasmid

   #170191

   Purpose

   Mammalian expression of SARS-CoV-2 spike receptor-binding domain (RBD), triple mutant from B.1.351 variant

   Depositor

   Dominic Esposito

   Article

   Mehalko et al Protein Expr Purif. 2021 Mar;179:105802.

   Insert

   SARS-CoV-2 RBD (S SARS-CoV-2)
   

   Use

   Tags

   His6

   Expression

   Mammalian

   Mutation

   K417N, E484K, N501Y

   Promoter

   CMV51

   Available since

   May 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. B9-COMP-blac-flag-his

   Plasmid

   #111020

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   6-cysteine protein (B9) (PF3D7_0317100 )
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Oct. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. PF3D7_1350600-COMP-blac-flag-his

    Plasmid

    #110968

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    PF3D7_1350600 (PF3D7_1350600 )
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GPR180-COMP-blac-flag-his

    Plasmid

    #110955

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    PF3D7_1213500 (PF3D7_1213500 )
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti-rInsp-hIns-GLuc

    Plasmid

    #89927

    Purpose

    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    410bp rat insulin II promoter

    Available since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1+(rInsp)-Ins-GLuc

    Plasmid

    #89928

    Purpose

    Expresses Insulin-GLuc from the rat insulin promoter. Gaussia Luc is inserted within the C-peptide and is co-secreted with insulin.

    Depositor

    Melanie Cobb

    Article

    Kalwat et al ACS Sens. 2016 Oct 28;1(10):1208-1212. E

    Insert

    human insulin-Gaussia-Luciferase (INS Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    DNA sequence for Gaussia luciferase was humanized…

    Promoter

    rat Insulin II promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKP332 (Lenti-OSK1)

    Plasmid

    #21627

    Depositor

    Tim Townes

    Article

    Chang et al Stem Cells. 2009 May . 27(5):1042-9.

    Insert

    hOCT4 - 2A - hSOX2 - 2A - hKLF4 (POU5F1, SOX2, KLF4 Porcine teschovirus-1, Human)
    

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. Rosa26-LSL-PE2 Targeting Vector

    Plasmid

    #196038

    Purpose

    The targeting construct contains a CAGG promoter, FRT site, loxP–stop-loxP, the PE2 enzyme, P2A peptide, an mNeonGreen reporter, WPRE, polyA signal, and a PGK-FRT3-NeoR-polyA.

    Depositor

    Tyler Jacks

    Article

    Ely et al Nat Biotechnol. 2023 May 11. doi: 10.103

    Insert

    Cas9-MMLV-RT
    

    Use

    Mouse Targeting

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGG

    Available since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDONR223-CSNK2A1

    Plasmid

    #23678

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2A1 (CSNK2A1 Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pDONR223-CSNK2B

    Plasmid

    #23359

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    CSNK2B (CSNK2B Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    C138T

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

18. pDONR223-PIK3CA

    Plasmid

    #23466

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PIK3CA (PIK3CA Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    A1035V

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

19. pDONR223-PDGFRB

    Plasmid

    #23893

    Depositor

    William Hahn, David Root

    Article

    Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

    Insert

    PDGFRB (PDGFRB Human)
    

    Use

    Gateway donor vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. GRP94-COMP-blac-flag-his

    Plasmid

    #111008

    Purpose

    Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

    Depositor

    Gavin Wright

    Article

    Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

    Insert

    endoplasmin, putative (GRP94) (PFL1070c Synthetic)
    

    Use

    Tags

    Exogenous signal peptide of mouse origin and rat …

    Expression

    Mammalian

    Mutation

    All NXT/S sites mutated to NXA, where X is any am…

    Promoter

    CMV

    Available since

    Oct. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge