We narrowed to 17,589 results for: She

1. pcDNA3-FLAG-MTOR-S2215Y

   Plasmid

   #69013

   Purpose

   activating MTOR mutation

   Depositor

   David Sabatini

   Article

   Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

   Insert

   MTOR (MTOR Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S2215Y

   Promoter

   CMV

   Available Since

   Jan. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-mCardinal

   Plasmid

   #51311

   Purpose

   Expresses far-red fluorescent protein mCardinal in mammalian cells

   Depositor

   Michael Lin

   Article

   Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

   Insert

   mCardinal
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 8, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.10/RSV_SF3B3-miniXon_Luciferase

   Plasmid

   #174660

   Purpose

   Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   miniXon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGL4.10/RSV_SF3B3-Xon_Luciferase

   Plasmid

   #174659

   Purpose

   Plasmid containing the SF3B3-Xon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-xon by LMI070.

   Depositor

   Beverly L. Davidson

   Article

   Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586

   Insert

   Xon cassette
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   Kozak ATG initiation sequence in middle exon

   Promoter

   RSV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSGV1-1D3-28Z.1-3 mut

   Plasmid

   #107227

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs mutated

   Depositor

   Steven Rosenberg, James Kochenderfer

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z. 1-3 mut
   

   Use

   Retroviral

   Tags

   None

   Mutation

   None

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTol2-hsp70l:Cas9-t2A-GFP, 5xU6:sgRNA

   Plasmid

   #108871

   Purpose

   Expression of heat shock inducible Cas9-GFP and U6-driven 5 individual gRNAs for scGESTALT in zebrafish

   Depositor

   Alex Schier

   Article

   Raj et al Nat Biotechnol. 2018 Mar 28. pii: nbt.41

   Inserts

   5xU6:sgRNA
   Cas9-t2A-GFP
   

   Use

   CRISPR

   Promoter

   U6 and hsp70

   Available Since

   April 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. MSGV-1D3-28Z All ITAMs intact

   Plasmid

   #107226

   Purpose

   Anti-mouse CD19 CAR with CD3 ITAMs intact

   Depositor

   Steven Rosenberg, James Kochenderfer

   Article

   Kochenderfer et al Blood. 2010 Nov 11;116(19):3875-86. doi:

   Insert

   1D3-28Z All ITAMs intact
   

   Use

   Retroviral

   Tags

   None

   Mutation

   None

   Available Since

   March 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pACTA2_HL-P2A-eGFP-PGK-PuroR-ACTA2_HR

   Plasmid

   #126705

   Purpose

   donor vector for targeting a 2A peptide followed by green fluorescent reporter to the human ACTA2 locus at the end of the endogenous coding sequence

   Depositor

   Darrell Kotton

   Article

   Kwong et al Stem Cell Reports. 2019 Sep 10;13(3):499

   Insert

   GFP
   

   Use

   CRISPR

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFUGW-FerH-ffLuc2-eGFP

   Plasmid

   #71393

   Purpose

   Lentiviral vector of luciferase-eGFP fusion gene driven by FerH promoter

   Depositor

   Glenn Merlino

   Article

   Day et al Pigment Cell Melanoma Res. 2009 Jun;22(3

   Insert

   FerH-ffLuc2-eGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   FerH

   Available Since

   Dec. 9, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-Ef1a-DIO eNpHR 3.0-EYFP

    Plasmid

    #26966

    Purpose

    AAV expression of eNpHR 3.0 fused to EYFP driven by EF1a promoter for optogenetic inhibition

    Depositor

    Karl Deisseroth

    Article

    Gradinaru et al Cell. 2010 Apr 2. 141(1):154-65.

    Has Service

    AAV Retrograde, AAV1, AAV5, and AAV9

    Insert

    eNpHR 3.0
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    ER export at 3' end of gene. Trafficking sig…

    Promoter

    EF1alpha

    Available Since

    Jan. 27, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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11. T7-VEE-mCherry

    Plasmid

    #193355

    Purpose

    Self-replicating RNA vector expressing mCherry fluorescent protein + PuroR

    Depositor

    Hans Schöler

    Article

    MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

    Insert

    mCherry
    

    Use

    Venezuelan equine encephalitis (vee) virus rna re…

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His-TEV-NQO1

    Plasmid

    #119058

    Purpose

    For expression in E.coli and affinity purification of N-terminally His tagged NQO1

    Depositor

    Yosef Shaul

    Article

    Tsvetkov et al Proc Natl Acad Sci U S A. 2005 Apr 12;10

    Insert

    NAD(P)H dehydrogenase, quinone 1 (NQO1) (NQO1 Human)
    

    Tags

    6His, TEV site

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-CAG-FLEX-SomArchon

    Plasmid

    #126943

    Purpose

    Cre-dependent expression of SomArchon under CAG promoter

    Depositor

    Edward Boyden

    Article

    Piatkevich et al Nature. 2019 Oct;574(7778):413-417. doi:

    Insert

    SomArchon
    

    Use

    AAV

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAVS1-PC-GCaMP6f

    Plasmid

    #73503

    Purpose

    GCaMP6f knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Insert

    GCaMP6f
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. LBR-mCherry

    Plasmid

    #172446

    Purpose

    Expression of nuclear envelope protein LBR with a C-terminal mCherry tag

    Depositor

    Stephen Royle

    Article

    Ferrandiz et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    lamin B receptor (LBR Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3-FLAG-MTOR-V2006I

    Plasmid

    #69012

    Purpose

    activating MTOR mutation

    Depositor

    David Sabatini

    Article

    Grabiner et al Cancer Discov. 2014 May;4(5):554-63. doi

    Insert

    MTOR (MTOR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    V2006I

    Promoter

    CMV

    Available Since

    Jan. 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLX304_zeo_empty

    Plasmid

    #160093

    Purpose

    Control lentiviral vector for mammalian gene expression

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRK5-HA-SLC38A9.1

    Plasmid

    #71869

    Purpose

    overexpression

    Depositor

    David Sabatini

    Article

    Wang et al Science. 2015 Jan 9;347(6218):188-94. do

    Insert

    SLC38A9.1 (SLC38A9 Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized

    Promoter

    CMV

    Available Since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pDONR221_EGFP

    Plasmid

    #25899

    Purpose

    Gateway entry vector containing EGFP.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    EGFP
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE hSPC-eGFP-W

    Plasmid

    #36450

    Depositor

    Darrell Kotton

    Article

    Longmire et al Cell Stem Cell. 2012 Apr 6;10(4):398-411

    Inserts

    human SP-C promoter
    eGFP (eGFP )
    

    Use

    Lentiviral

    Promoter

    N/A and human SP-C promoter

    Available Since

    May 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pME-H2B-mCardinal (JDW 1418)

    Plasmid

    #242553

    Purpose

    Gateway middle entry clone containing H2B-mCardinal; Nuclear far red fluorescent reporter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    mCardinal
    

    Use

    Gateway subcloning

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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22. pRT043-CLYBL-DDdcas9-VPH

    Plasmid

    #158091

    Purpose

    Inducible expression of dCas9-VPH from the CLYBL locus for CRISPR activation

    Depositor

    Martin Kampmann

    Article

    Tian et al Nat Neurosci. 2021 May 24. pii: 10.1038/

    Insert

    CLYBL-CAG-DDdCas9VPH-T2A-GFP
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pminiTol2-4kb wnt3 promoter-Wnt3EGFP

    Plasmid

    #203793

    Purpose

    Tol2 based genomic integration of zebrafish 4kb Wnt3 promoter driving the expression of Wnt3-EGFP for zebrafish transgenesis

    Depositor

    Thorsten Wohland

    Article

    Teh et al Development. 2015 Nov 1;142(21):3721-33.

    Insert

    Wnt3 (wnt3 Zebrafish)
    

    Use

    Zebrafish expression

    Tags

    mEGFP

    Promoter

    4kb Wnt3 promoter

    Available Since

    Nov. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. tet-pLKO shGATA6 puro

    Plasmid

    #72615

    Purpose

    Expresses an inducible short hairpin targeting human GATA6 sequence

    Depositor

    Kevin Janes

    Article

    Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi:

    Insert

    short hairpin GATA binding protein 6
    

    Use

    Lentiviral

    Tags

    None

    Expression

    Mammalian

    Promoter

    TRE Tight

    Available Since

    Aug. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Available Since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-mNeptune2.5

    Plasmid

    #51310

    Purpose

    Expresses far-red fluorescent protein mNeptune2.5 in mammalian cells

    Depositor

    Michael Lin

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    mNeptune2.5
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 8, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV5-Flag-HDAC3

    Plasmid

    #63676

    Purpose

    Mammalian expression of Flag tagged HDAC3

    Depositor

    Lingfang Zeng, Qingbo Xu

    Article

    Zeng et al J Cell Biol. 2006 Sep 25;174(7):1059-69.

    Insert

    HDAC3 (Hdac3 Mouse)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

29. pBabe_puro_DEST_Flag_SKIL

    Plasmid

    #45265

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

30. pAAV-SYN-sDarken

    Plasmid

    #184796

    Purpose

    Serotonin Sensor sDarken under the control of a hSyn Promoter.

    Depositor

    Olivia Masseck

    Article

    Kubitschke et al Nat Commun. 2022 Dec 6;13(1):7525. doi:

    Insert

    sDarken
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pDL025_HA-rb_IgG_HC_15F11

    Plasmid

    #193622

    Purpose

    HA-tag heavy chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL026.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti_HA_15F11 rabbit heavy chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

32. pDL026_HA-rb_IgG_LC_15F11

    Plasmid

    #193623

    Purpose

    HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

    Depositor

    Jennifer DeLuca

    Article

    DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

    Insert

    Anti-HA_15F11 rabbit light chain
    

    Tags

    signal peptide

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 21, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

33. pLPCX-VEcadTS

    Plasmid

    #45848

    Depositor

    Martin Schwartz

    Article

    Conway et al Curr Biol. 2013 May 14. pii: S0960-9822(

    Insert

    VE-cadherin Tension Sensor (Cdh5 Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. AICSDP-43: GJA1-mEGFP

    Plasmid

    #109120

    Purpose

    Homology arms and linker-mEGFP sequence for C-terminus tagging of human GJA1

    Depositor

    Allen Institute for Cell Science

    Insert

    GJA1 Homology Arms with linker-mEGFP (GJA1 Human)
    

    Use

    CRISPR; Donor template

    Tags

    linker-mEGFP

    Expression

    Mammalian

    Mutation

    homology arms contain point mutations to disrupt …

    Available Since

    May 4, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

35. pDONR223_LacZ

    Plasmid

    #25893

    Purpose

    Gateway entry vector containing LacZ.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Insert

    LacZ
    

    Use

    Gateway entry vector

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pLX303

    Plasmid

    #25897

    Purpose

    3rd generation lentiviral Gateway destination vector. Blasticidin selection.

    Depositor

    David Root

    Article

    Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Expression

    Mammalian

    Available Since

    July 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. siRNA-resistant SigmaR1-mNeonGreen R119A F191A

    Plasmid

    #226574

    Purpose

    Encodes mutant siRNA resistant SigmaR1 protein (substitutions) labeled with mNeonGreen

    Depositor

    Gia Voeltz

    Article

    Sawyer et al Dev Cell. 2024 Jul 2:S1534-5807(24)00382

    Insert

    SigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    R119A, F191A

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)

    Plasmid

    #177850

    Purpose

    Bacterial Expression of AMPK

    Depositor

    Karsten Melcher

    Article

    Yan et al Science. 2021 Jul 23;373(6553):413-419.

    Insert

    AMPK (a1b2g1)
    

    Tags

    MBP

    Expression

    Bacterial

    Available Since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pmCherry-ATG5

    Plasmid

    #13095

    Depositor

    Roberta Gottlieb

    Article

    Hamacher-Brady et al Cell Death Differ. 2007 Jan;14(1):146-57

    Insert

    ATG5 (Atg5 Mouse)
    

    Tags

    mCherry

    Expression

    Mammalian

    Available Since

    Nov. 15, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pSLQ4419 pHR (hU6-BsmBI-BsmBI-EFS-PuroR-WPRE)

    Plasmid

    #214875

    Purpose

    Empty lentiviral backbone for guide array cloning

    Depositor

    Stanley Qi

    Article

    Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    April 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge