We narrowed to 143,272 results for: ins

1. Venus-1-155-Ggamma2

   Plasmid

   #234752

   Purpose

   Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

   Depositor

   Nevin Lambert

   Article

   Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

   Insert

   GNG2 (GNG2 Human)
   

   Use

   Tags

   VC155

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Venus-155-239-Gbeta1

   Plasmid

   #234751

   Purpose

   Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

   Depositor

   Nevin Lambert

   Article

   Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

   Insert

   GNB1 (GNB1 Human)
   

   Use

   Tags

   VN155

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pdCas9-GFP-p65

   Plasmid

   #232630

   Purpose

   p65 activation domain coupled to catalytically inactive dCas9 for localization; contains GFP for labeling and as a spacer

   Depositor

   Karsten Rippe

   Insert

   dCas9-GFP-p65 (RELA Synthetic, Human)
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Efg1-ΔDBD-Y115S/Y132S/Y139S

   Plasmid

   #216408

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y115/Y132/Y139 to serine mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-Y115S/Y132S/Y139S
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Tyrosine 115, 132 and 139 to Serine, and …

   Promoter

   Available since

   July 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV DJ-1 C106A

   Plasmid

   #220914

   Purpose

   Expresses DJ-1 with the C106A mutation

   Depositor

   Timothy Ryan

   Article

   Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

   Insert

   DJ-1 (PARK7 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   C106A

   Promoter

   CMV

   Available since

   June 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pITEu2

   Plasmid

   #218282

   Purpose

   Use together with pToxEXP/pToxAmp plasmids to insert heterologous genes at ho locus

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat2-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJE13HD7

   Plasmid

   #218281

   Purpose

   Use together with pITEdv1, or pToxEXP1/pToxAmp plasmids to insert heterologous gene(s) at ho locus.

   Depositor

   Claudia Vickers

   Article

   Evans et al bioRxiv 2024.01.30.578080

   Insert

   HO(-253, -1)-Repeat1-ISceI-pAgTEF1>Hph(partial)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mFzd4CRDL(42-203)-6xHis_pAcGP67a

   Plasmid

   #216379

   Purpose

   Baculovirus transfer vector to secrete Fzd4 cysteine-rich domain and linker (residues 42-203, codon-optimized for insect cell expression, with a C-terminal His tag) in insect cells

   Depositor

   William Weis

   Article

   Bruguera et al bioRxiv 2024.02.03.578714

   Insert

   Frizzled-4 (Fzd4 Mouse)
   

   Use

   Baculovirus

   Tags

   6xHis and GP64 signal sequence

   Expression

   Insect

   Mutation

   Promoter

   Polyhedrin

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Efg1-ΔDBD-A51P/A56P/Q443P

   Plasmid

   #216402

   Purpose

   Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with A51/A56/Q443 to proline mutations

   Depositor

   Nicolas Fawzi

   Article

   Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

   Insert

   Efg1-ΔDBD-A51P/A56P/Q443P
   

   Use

   Tags

   6xHis-tags

   Expression

   Bacterial

   Mutation

   changed Alanine 51, 56 and Glutamine 443 to Proli…

   Promoter

   Available since

   April 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Efg1-ΔDBD-Y4S/Y8S/Y16S

    Plasmid

    #216405

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y4/Y8/Y16 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y4S/Y8S/Y16S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 4, 8 and 16 to Serine, and delet…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Efg1-ΔDBD-Y64S/Y66S/Y69S

    Plasmid

    #216406

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y64/Y66/Y69 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y64S/Y66S/Y69S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 64, 66 and 69 to Serine, and del…

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Efg1-ΔDBD-Y101S/Y104S/Y106S

    Plasmid

    #216407

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y101/Y104/Y106 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y101S/Y104S/Y106S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 101, 104 and 106 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Efg1-ΔDBD-Y401S/Y404S/Y409S

    Plasmid

    #216409

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y401/Y404/Y409 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y401S/Y404S/Y409S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 401, 404 and 409 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Efg1-ΔDBD-Y450S/Y452S/Y456S

    Plasmid

    #216410

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y450/Y452/Y456 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y450S/Y452S/Y456S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 450, 452 and 456 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Efg1-ΔDBD-Y478S/Y482S/Y484S

    Plasmid

    #216411

    Purpose

    Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1 with Y478/Y482/Y484 to serine mutations

    Depositor

    Nicolas Fawzi

    Article

    Wang et al bioRxiv [Preprint]. 2023 Nov 9:2023.11.0

    Insert

    Efg1-ΔDBD-Y478S/Y482S/Y484S
    

    Use

    Tags

    6xHis-tags

    Expression

    Bacterial

    Mutation

    changed Tyrosine 478, 482 and 484 to Serine, and …

    Promoter

    Available since

    April 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pBC135

    Plasmid

    #202262

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc162 through Tn7 bacterial insertion, Kanamycin selectable marker and tagBFP fluorescent marker

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc162
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBC113

    Plasmid

    #202275

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc140 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc140
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBC115

    Plasmid

    #202277

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc142 through Tn7 bacterial insertion, Tetracyline selectable marker and far-red-FP fluorescent marker mPlum

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc142
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBC117

    Plasmid

    #202278

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc144 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc144
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBC118

    Plasmid

    #202279

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc145 through Tn7 bacterial insertion, Tetracyline selectable marker and GFP fluorescent marker

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc145
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge