We narrowed to 156,064 results for: ins
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Plasmid#118759PurposeVPS13A internally tagged with Halo at residue 1372.DepositorAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only
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pET32a-hTGFa
Plasmid#199236PurposeBacterial production of soluble, active target proteins; Nterm thrombin and enterokinase cleavage sites.DepositorInsertTransforming growth factor alpha, partial (TGFA Human)
TagsTrxA-6xHis-S-tagExpressionBacterialPromoterT7Available SinceMay 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked Free NES (M)
Plasmid#182436PurposeYeast integrative plasmid for expressing fusion protein ERG20(F96W-N127W)-AcNES1 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertFPPS(M)-NES
UseSynthetic Biology; Metabolic engineeringExpressionYeastPromoterGAL10Available SinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSEM319 - array co-injection fragment mosTI unc-119
Plasmid#159823PurposeSingle copy or array insertion by MosTI using split unc-119 selectionDepositorInsertarray co-injection fragment mosTI unc-119
ExpressionWormMutationNot applicableAvailable SinceDec. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pQE-80L iLID (C530M)
Plasmid#60408PurposeE. coli expression of iLID (C530M)DepositorInsertiLID C530M
TagsHisExpressionBacterialMutationC530MAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pME-ReaChR-citrine
Plasmid#169045PurposeMiddle entry vector for Gateway cloning that contains a ReaChR fused with citrine gene insertDepositorInsertReaChR-citrine
UseGateway entry cloning vectorTagscitrineAvailable SinceJan. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGEX PLCg1(N)-SH2
Plasmid#46470DepositorInsertPLCg1(N)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 539-664PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mNeptune2-N
Plasmid#41645PurposeExpresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)DepositorInsertmNeptune2-Dronpa145N
ExpressionMammalianMutationK145N on Dronpa (allows control of photochromic F…PromoterCMVAvailable SinceDec. 21, 2012AvailabilityAcademic Institutions and Nonprofits only -
pAct:dCas9-VPR
Plasmid#78898PurposeExpresses dCas9-VPR under Actin promoter for CRISPRa in Drosophila cell culture. Please note- this plasmid does not express GFP.DepositorInsertdCas9-VPR
UseCRISPRExpressionInsectPromoterpActin (Drosophila)Available SinceAug. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLenti-tetON-NKX2-1-EF1a-TagRFP-2A-tet3G
Plasmid#167942PurposeInducible NKX2-1 overexpressionDepositorAvailable SinceJune 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChRmine-mRuby2-ST
Plasmid#227777PurposeCation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitationDepositorInsertChRmine-mRuby2-ST
ExpressionMammalianAvailable SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0: tgRFPt-SSPB R73Q
Plasmid#60416PurposeMammalian Expression of tgRFPt-MicroDepositorInserttgRFPt-SSPB R73Q
UseCre/Lox and LentiviralTagsTagRFPtExpressionMammalianMutationR73Q, Y11K and A15EPromoterCMVAvailable SinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pECIA2
Plasmid#47032PurposeThis is a Gateway destination vector for secreted expression in Drosophila cell culture by induction via CuSO4. Inserts will be C-terminally tagged with Fc, V5 and hexahistidine tags.DepositorTypeEmpty backboneUseSecreted expression in drosophila cultureTagsFc(human IgG1), HRV 3C Protease Cleavage site, He…ExpressionInsectPromoterMetallothionein (Copper-Inducible)Available SinceAug. 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pDV24_AAV2_DART-VADAR_iRFP720-sensor
Plasmid#200411PurposeAAV vector encoding a DART-VADAR sensor for iRFP720 mRNADepositorInsertDART VADAR sensor
UseAAV and Synthetic BiologyTagsMCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreenExpressionMammalianPromoterCMVAvailable SinceJune 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBS human IRS-1
Plasmid#11359DepositorInsertIRS-1 (IRS1 Human)
ExpressionBacterialAvailable SinceMarch 1, 2006AvailabilityAcademic Institutions and Nonprofits only -
pMKO.1 puro GFP shRNA
Plasmid#10675PurposeNegative control vector for pMKO.1 puro (Addgene plasmid #8452); Contains shRNA against GFP.DepositorInsertGFP shRNA
UseRNAi and RetroviralExpressionMammalianAvailable SinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits only -
SQT1665
Plasmid#78744PurposeHuman expression plasmid for LbCpf1-NLS-3xHADepositorInsertmammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA
UseCRISPRTagsNLS-3xHAExpressionMammalianPromoterCAGAvailable SinceJune 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTK331
Plasmid#59572PurposeTC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceIDepositorInsertSGP(16) 2xK-turn Kozak EGFP attB2 AscI
Available SinceAug. 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAG-ChroMD-mRuby2-ST
Plasmid#171158PurposeCation channelrhodopsin ChroMD fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendritesDepositorInsertChroMD
ExpressionMammalianAvailable SinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
dCas9-10xSunTag-P2A-tagBFP
Plasmid#174141PurposeExpresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.DepositorInsertdCas9, 10xSunTag, tagBFP
TagsHA and tagBFPExpressionMammalianPromoterSV40Available SinceOct. 22, 2021AvailabilityAcademic Institutions and Nonprofits only