We narrowed to 20,463 results for: Cre

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actc1b-mKate2-Rac1-DN

Plasmid

#109530

Purpose

Muscle specific zebrafish expression of mKate2 fused to dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Tags

mKate2

Mutation

DN

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a(+)-SUMO-A'

Plasmid

#209115

Purpose

Bacterial expression of CC-GEMS ligand SUMO-A'

Depositor

Tom de Greef

Article

Makri Pistikou et al Nat Commun. 2023 Nov 2;14(1):7001. doi:

Insert

SUMO-A'

Expression

Bacterial

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pYPQAT25

Plasmid

#223397

Purpose

T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.

Depositor

Yiping Qi

Article

Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

Insert

AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0

Use

CRISPR

Tags

3x FLAG and NLS

Expression

Plant

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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RoL10X_pET29b

Plasmid

#215427

Purpose

Expression construct for RoL10X gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

RoL10X

Expression

Bacterial

Mutation

V209L/D262G/E190C/E238C/F173Y/V175F/G177C/Q197H/F…

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGWB545

Plasmid

#74879

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRSV-CaMKII

Plasmid

#45064

Purpose

Expression of CaMKII

Depositor

Richard Maurer

Article

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

CaM Kinase II (Camk2a Rat)

Expression

Mammalian

Promoter

RSV

Available Since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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CMV500 A-C/EBP

Plasmid

#33352

Depositor

Charles Vinson

Article

Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

Insert

C/EBP (Cebpa Mouse)

Tags

FLAG

Expression

Mammalian

Mutation

Dominant Negative (see notes)

Promoter

CMV

Available Since

Dec. 8, 2011

Availability

Academic Institutions and Nonprofits only
pLV-REPORT(PGK/CMV)-GRE

Plasmid

#172336

Purpose

Gal4 Response element

Depositor

David Bersten

Article

Allen et al bioRxiv 2023.11.21.568191

Inserts

monomeric nuclear Tomato
d2eGFP
HygR

Use

Lentiviral and Synthetic Biology

Tags

3x SV40 NLS

Expression

Mammalian

Promoter

PGK/CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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PBMN YFP-ER50

Plasmid

#37261

Depositor

Thomas Wandless

Article

Miyazaki et al J Am Chem Soc. 2012 Mar 7;134(9):3942-5.

Insert

ER50 (ESR1 Human)

Use

Retroviral

Tags

HA-YFP

Mutation

6 mutations, T371A, L384M, M421G, G521R, Y537S, N…

Promoter

LTR

Available Since

Aug. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pME-mKate2-KDEL-pA

Plasmid

#109546

Purpose

Multisite gateway vector for expression of mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence (marks the ER, or ER/SR in muscle)

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

mKate2 fused to the 5' signal sequence from calreticulin and 3' KDEL ER retention sequence

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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PARP2-c098

Plasmid

#216873

Purpose

Bacterial expression vector for E. coli producing PARP2 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

Insert

PARP2 catalytic domain (no Regulatory Domain) (PARP2 Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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CA-0192 CMVTO-Renin(mouse)-ECL-CD4-tevs-RXR6.2

Plasmid

#231204

Purpose

ER-retained mouse renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(mouse)-ECL-CD4-tevs-RXR6.2 (Ren1 Mouse)

Expression

Mammalian

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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CA-0193 CMVTO-Renin(H2M)-ECL-CD4-tevs-RXR6.2

Plasmid

#231203

Purpose

ER-retained H2M renin

Depositor

Xiaojing Gao

Article

Aldrete et al Nat Chem Biol. 2025 Jan 15. doi: 10.1038

Insert

Renin(H2M)-ECL-CD4-tevs-RXR6.2 (REN Human)

Expression

Mammalian

Mutation

L147I, R148H, I203V, L290V

Available Since

Jan. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSL1180-HR-PUbEBFP2-NLS

Plasmid

#176682

Purpose

Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

Depositor

Norbert Perrimon

Article

Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

Insert

EBFP2

Expression

Insect

Promoter

Ae. aegypti poly-ubiquitin (AAEL003888)

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESbsdpA

Plasmid

#112922

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Blasticidine resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP94, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Expression

Mammalian

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB521

Plasmid

#74863

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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LB701: pMVP/PB/mCherry-DEST

Plasmid

#121871

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ mCherry selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pGWB520

Plasmid

#74862

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 10xMyc (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Sept. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV7.1_3xFLAG-WWC3

Plasmid

#172996

Purpose

constitutive expression of FLAG-tagged WWC3

Depositor

Michael Wehr

Article

Ma et al Cell Signal. 2024 Jan;113:110917. doi: 1

Insert

3xFLAG-WWC3 (WWC3 Human)

Tags

3xFLAG

Expression

Mammalian

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGWB409

Plasmid

#74803

Purpose

Gateway cloning compatible binary vector for N-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Nov. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pGWB410

Plasmid

#74804

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with FLAG (no promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Available Since

July 14, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-G6-vioABECD

Plasmid

#66536

Purpose

pETM6 vector containing vioA-vioB-vioE-vioC-vioD in monocistronic configuration with G6 promoter

Depositor

Mattheos Koffas

Article

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Inserts

VioA
VioB
VioE
VioC
VioD

Use

Synthetic Biology

Expression

Bacterial

Promoter

Mutant T7 Promoter - G6

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pLenti-U6sgbbBsmbI-puro-2A-Fluc

Plasmid

#100277

Purpose

Lentiviral vector for CRISPR mediated gene editing in cells while expressing Firefly Luciferase for in vivo imaging

Depositor

Sidi Chen

Article

Chow et al Nat Neurosci. 2017 Aug 14. doi: 10.1038/

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Oct. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pGWB508

Plasmid

#74850

Purpose

Gateway cloning compatible binary vector for C-terminal fusion with 6xHis (CaMV35S promoter).

Depositor

Tsuyoshi Nakagawa

Article

Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

Type

Empty backbone

Expression

Plant

Promoter

CaMV35S

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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LC301: pMVP/PB/Zeo-DEST

Plasmid

#121877

Purpose

pMVP destination vector, empty PiggyBac transposon vector backbone w/ Zeo selection cassette

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Piggybac transposon, pmvp gateway destination vec…

Expression

Mammalian

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYT7alpha-HaloTag

Plasmid

#179715

Purpose

Lentiviral expression of mouse Syt7 with C terminal HaloTag

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBK-YE2-BE3

Plasmid

#85176

Purpose

Expresses YE2-BE3 in mammalian cells

Depositor

David Liu

Article

Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

YEE-BE3

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pRDA_085

Plasmid

#158583

Purpose

Lentiviral expression of SpCas9; P2A site provides mKate2

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

SpCas9

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pAT9650-BEAR-GFP-preedited

Plasmid

#162992

Purpose

BEAR control plasmid with split EGFP and intact 5' splice site

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

EGFP split with an intron between amino acids 95-96

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6013x

Plasmid

#108654

Purpose

An HRas biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

Raichu-EV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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