Addgene

Showing: 521 - 540 of 12399 results

Results per page:

Sort results by:

pIZ-EGFP-MYO10-HMM

Plasmid

#87257

Purpose

Expression plasmid to perform NanoSPD assays in insect cells (generates EGFP-tagged bait).

Depositor

Thomas Friedman

Article

Bird et al Mol Biol Cell. 2016 Dec 8. pii: mbc.E16-

Insert

MYO10 (Myo10 Mouse)

Use

Tags

Expression

Insect

Mutation

Myo10 is truncated to contain aa.1-941

Promoter

OpIE2

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
AAV-shRNA-ctrl

Plasmid

#85741

Purpose

shRNA ctrl

Depositor

Hongjun Song

Article

Yu et al Nat Neurosci. 2015 Jun;18(6):836-43. doi

Insert

shRNA ctrl

Use

AAV

Tags

EYFP

Expression

Mutation

Promoter

Available since

April 22, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pSCAR_Cas9-hygro_GFP

Plasmid

#162075

Purpose

3rd generation lentivirus transfer vector for Cre-removable Cas9, GFP reporter, and hygro resistance

Depositor

Robert Manguso

Article

Dubrot et al Immunity. 2021 Jan 20. pii: S1074-7613(2

Inserts

Cas9-2a-hygromycin B phosphotransferase
EGFP

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mammalian

Mutation

codon-optimized for expression in M. musculus

Promoter

EF1a and sv40

Available since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW-TetO-Junb

Plasmid

#178441

Purpose

doxycycline-inducible expression of Mouse Junb in mammalian cells

Depositor

Filipe Pereira

Article

Rosa et al Sci Immunol. 2022 Mar 4;7(69):eabg5539.

Insert

Junb (Junb Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

March 14, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
TetO-Ascl1-puro

Plasmid

#97329

Purpose

Generating lentiviruses to express Ascl1 and puromycin resistance

Depositor

Marius Wernig

Article

Yang et al Nat Methods. 2017 May 15. doi: 10.1038/n

Insert

Ascl1 (Ascl1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TET-ON

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pROSA26-1

Plasmid

#21714

Depositor

Philippe Soriano

Article

Soriano et al Nat Genet. 1999 Jan;21(1):70-1.

Insert

ROSA26 (Gt(ROSA)26Sor Mouse)

Use

Mouse Targeting

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-FLAG-mTUT3

Plasmid

#60040

Purpose

Mammalian expression of FLAG-tagged mouse TUT3

Depositor

Zissimos Mourelatos

Article

Liu et al Mol Cell. 2014 Aug 27. pii: S1097-2765(1

Insert

TUT3 (Papd5 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 4, 2014

Availability

Academic Institutions and Nonprofits only
pAAV.GFAP.(Cyto)iGlucoSnFR.mRuby2

Plasmid

#164514

Purpose

Green glucose sensor with mRuby2 fusion, cytosolic

Depositor

Loren Looger

Article

Keller et al bioRxiv (2019) 571422; doi: https://doi.

Insert

(Cyto)iGlucoSnFR-mRuby2

Use

AAV

Tags

Expression

Mutation

Synthetic Construct

Promoter

GFAP

Available since

Feb. 5, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MYD88

Plasmid

#13092

Depositor

Ruslan Medzhitov

Insert

MYD88 (Myd88 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPRv2_mSTING_gRNA_2

Plasmid

#196626

Purpose

Knock-out STING in murine cells, gRNA 2.

Depositor

Nicolas Manel

Article

Jneid et al Sci Immunol. 2023 Jan 13;8(79):eabn6612.

Insert

mSTING gRNA 2 (Sting1 Mouse)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

WT

Promoter

Available since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
EFS-SpdCas9-Dnmt3A/3L-WT

Plasmid

#222497

Purpose

Expresses EFS promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L followed by P2A-mCherry

Depositor

Isaac Hilton

Article

Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

Insert

S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

EFS

Available since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRp5-p2 GFP-NShroom3-iLID

Plasmid

#170976

Purpose

N-terminal component of OptoShroom3 optogenetic tool. Binds to apical junctions. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

NShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

eGFP and iLID

Expression

Mutation

From isoform 2, deleted amino acids 1389 - 1805,…

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONRp5-p2 SspB-mCherry-CShroom3

Plasmid

#170977

Purpose

C-terminal component of OptoShroom3 optogenetic tool. Translocates to apical junctions to induce constriction upon blue light illumination if coexpressed with GFP-NShroom3-iLID. pDONRp5-p2 plasmid.

Depositor

Miki Ebisuya

Article

Martinez-Ara et al Nat Commun. 2022 Sep 14;13(1):5400. doi:

Insert

CShroom3 (Shroom3 Mouse)

Use

Gateway cloning

Tags

SspB and mCherry

Expression

Mutation

Isoform X2, deleted aminoacids 1 - 1388

Promoter

Available since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-Ef1a-fDIO-eGFP-2A-Cre-WPRE

Plasmid

#203842

Purpose

Flp-dependent Cre and EGFP expression

Depositor

Ivo Spiegel

Article

Roethler et al Neuron. 2023 Jun 20:S0896-6273(23)00399-

Insert

Cre

Use

AAV

Tags

EGFP-2A

Expression

Mutation

Promoter

Available since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBS PKC theta

Plasmid

#8426

Depositor

Frederic Mushinski

Article

Mischak et al FEBS Lett 1993 Jul 12;326(1-3):51-5.

Insert

Mouse Protein Kinase C theta (Prkcq Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 22, 2005

Availability

Academic Institutions and Nonprofits only
pAAV-CMV-N-HA-Pcdh15_mini_V4

Plasmid

#199185

Purpose

An AAV plasmid designed to facilitate the expression of mini-PCDH15 in the inner ear of a mouse.

Depositor

David Corey

Article

Ivanchenko et al Nat Commun. 2023 Apr 26;14(1):2400. doi:

Insert

HA-mmPcdh15_mini_V4-CD2

Use

AAV

Tags

Human influenza hemagglutinin (HA)

Expression

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pMIH-TFAM-GFP

Plasmid

#113704

Purpose

Fluorescent fusion protein used to visualise mitochondrial nucleoids, with hygromycin selection

Depositor

Benjamin Kile

Article

McArthur et al Science. 2018 Feb 23;359(6378). pii: 359

Insert

TFAM (Tfam Mouse)

Use

Retroviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

MSCV

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CAG-emiRFP670-P2A-GFP

Plasmid

#197160

Purpose

Expresses the protein of emiRFP670-P2A-GFP in mammalian cells

Depositor

Kiryl Piatkevich

Article

Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

Insert

emiRFP670-P2A-GFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pZac2.1 gfaABC1D-Aqp4-BioID2-BioID2-HA

Plasmid

#176742

Purpose

Expresses Aquaporin-4 fused with BioID2 at the C-terminus in astrocytes

Depositor

Baljit Khakh

Article

Soto et al Nature. 2023 Apr;616(7958):764-773. doi:

Insert

Aqp4-BioID2-BioID2-HA

Use

AAV

Tags

HA

Expression

Bacterial

Mutation

Promoter

gfaABC1D

Available since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHR_TRE_mcherry-P2A-Pcad

Plasmid

#133807

Purpose

inducible expression of mCherry and Pcad in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2018 Jul 13;361(6398):156-162.

Insert

cadherin 3 (CDH3)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge