We narrowed to 1,337 results for: piggyBac
-
Plasmid#133383PurposePiggybac vector for constitutive expressionDepositorInsertIGF2 (IGF2 Human)
ExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available SinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-TetR-tdTomato
Plasmid#212958PurposepiggyBac donor vector containing TetR-tdTomato for random integration by PBaseDepositorInsertTetR-tdTomato
UseMouse Targeting; Piggybase integration (transposo…TagstdTomatoExpressionMammalianAvailable SinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJT396 PB ITR and Cp36 attD pEF Puro-2a-tagBFP2 donor
Plasmid#184952PurposeDonor for both PiggyBac and Cp36 RecombinaseDepositorInsertPuroR, P2A, mTagBFP2
ExpressionMammalianAvailable SinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EFS-Cas9-BlastR
Plasmid#115146PurposeSafe harbor site 231 knock-in vector with Cas9-BlastR expression cassetteDepositorInsertCas9-T2A-BlastR
UseCRISPRExpressionMammalianPromoterEF1aAvailable SinceOct. 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
ExpressionBacterial and MammalianPromoterCAGAvailable SinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
AE-Polyubiquitin-GCaMP6s
Plasmid#106868PurposeExpresses polyubiquitin GCaMP6s, a genetically encoded calcium indicator, for live calcium imaging in Aedes aegypti mosquitoes.DepositorInsertAE-Polyubiquitin-GCAaMP6s
ExpressionInsectPromoterAe. aegypti polyubiquitin (PUb)Available SinceMay 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMB953:PB-CAG-PuroR-nGFP-FLEx-mCherry-KASH-WPRE
Plasmid#168106PurposePiggyBac transposon vector constitutively driving PuroR-P2A-eGFP and Cre conditional expression of KASH nuclear membrane-bound mCherry. Conditional insert flanked by MCS for cloningDepositorInsertmCherry-KASH
UseCRISPR and Cre/LoxPromoterCAGAvailable SinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
PB-CAG-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102887PurposePiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterCAGAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xU6-PylT M15 (UUA) sfGFP 150TAA
Plasmid#154775Purposeplasmid with 4xMma PylT (M15 mutant, UUA anticodon) cassette and ochre suppression reporter sfGFP 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAA in GFP reporter, UUA anticodon and G10A, …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG_NLS-Cas9-XTEN-Ec73RT-NLS_pA_pCMV_BFP_pA
Plasmid#176458PurposeVector for encoding a human codon-optimized SpCas9-XTEN-Ec73RT fusion driven by CAG promoter and tagBFP driven by CMV promoterDepositorInserthumanized S. pyogenes Cas9-XTEN-Ec73RT fusion
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pB-RTP2-Integration
Plasmid#129459PurposepiggyBac transposon vector that Inducibly (Tet-On) expresses RTP2DepositorAvailable SinceAug. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAG 150TAA
Plasmid#154776Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAG 150 TAA stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAG 150TAA in GFP reporter, hybrid PylT with A…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 150TAG
Plasmid#154772Purposeplasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation150TAG in GFP reporter, hybrid PylT with A41AA an…PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pB-TAG-HES7
Plasmid#130935PurposePiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cellsDepositorAvailable SinceOct. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xhyb PylT A41AA C55A sfGFP 102TAA 150TAG
Plasmid#154777Purposeplasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and dual suppression reporter sfGFP 102TAA 150 TAG stop, for transient transfection or stable, piggybac-mediated, integrationDepositorInsertsfGFP
ExpressionMammalianMutation102TAA 150TAG in GFP reporter, hybrid PylT with …PromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAS_4xG1 PylT FLAG-G1 PylRS
Plasmid#154768Purposeplasmid with 4xG1 PylT cassette and G1 PylRS for amber suppression in transient or stable, piggybac-mediated, integrationDepositorInsertG1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
TagsFLAGExpressionMammalianPromoterEF1Available SinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPH-T2A-GFP-IRES-Neo
Plasmid#102890PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
MTK0_015
Plasmid#123930PurposeEncodes the piggyBac GFP dropout destination vector with the 2xHS4 insulator and constitutive NLS::tagBFP as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - Insulator + tagBFP
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo
Plasmid#102891PurposePiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selectionDepositorInsertDDdCas9VP192-P300-T2A-GFP-IRES-Neo
UseCRISPRExpressionMammalianMutationD10A, H840APromoterTRE-tightAvailable SinceJuly 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSH231-EF1-BLST-Cas9-VPR
Plasmid#115147PurposeSafe harbor site 231 knock-in vector with BlastR-Cas9-VPR expression cassetteDepositorInsertBlastR-P2A-Cas9-VPR
UseCRISPRTagsSV40NLSExpressionMammalianPromoterEF1aAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
MTK0_043
Plasmid#123943PurposeEncodes the piggyBac GFP dropout destination vector with the A1 insulator as a type 0 part to be used in the MTK systemDepositorInsertPB Destination - A1 Insulator - HygroR
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-112–858_Flag2-Blast
Plasmid#175039PurposeMammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-1–729_Flag2-Blast
Plasmid#175040PurposeMammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein from amino acid positi…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPB-MPP8-∆ARD_Flag2-Blast
Plasmid#175041PurposeMammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tagDepositorInsertMphosph8 (Mphosph8 Mouse)
UsePiggybacTagsFlag2ExpressionMammalianMutationExpression of MPP8 protein with deletion of the a…PromoterCAGAvailable SinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
JPF0522
Plasmid#124013PurposeEncodes crippled pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vectorDepositorInsertPB_pEF1ac-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA
ExpressionMammalianAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJL308
Plasmid#243704PurposeA piggyBac vector containing the NDUFB9 coding sequence under the control of its endogenous promoter and 5' UTR, followed by the ACTB 3' UTRDepositorInsertNDUFB9 coding sequence with endogenous 5' UTR, followed by the ACTB 3' UTR (NDUFB9 Human)
ExpressionMammalianPromoterendogenous promoterAvailable SinceOct. 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-ETV2
Plasmid#225051PurposePiggyBac vector encoding doxycycline-inducible codon-optimized human ETV2DepositorInsertETS Variant Transcription Factor 2 (ETV2 Human)
ExpressionMammalianMutationCodon OptimizedPromoterTRE3GAvailable SinceJuly 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-DEST (JDW 1205)
Plasmid#229821PurposeA PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Neo-DEST (JDW 940)
Plasmid#229825PurposeA neo selectable, PiggyBac destination vector compatible with gateway cloning system.DepositorTypeEmpty backboneExpressionMammalianAvailable SinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR1 mutant WEVL -> AEVA)
Plasmid#229742PurposeExpresses Halo tagged human LRRK2 with LIR1 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR1 mutant WEVL -> AEVAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-HALO-hLRRK2 (LIR2 mutant WTFI -> ATFA)
Plasmid#229743PurposeExpresses Halo tagged human LRRK2 with LIR2 point mutations in mammalian cells. Stable expression with piggybac transposase.DepositorInsertLRRK2 (LRRK2 Human)
TagsHaloExpressionMammalianMutationLIR2 mutant WTFI -> ATFAPromoterEF1AAvailable SinceFeb. 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB-NeoCOVGT3-d2EGFP
Plasmid#201446PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT3_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP
Plasmid#201447PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP
Plasmid#201448PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT4-d2EGFP
Plasmid#201449PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT4_d2EGFP
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-NeoCOVGT3-d2EGFP-mCherry
Plasmid#201450PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT3_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT1-d2EGFP-mCherry
Plasmid#201451PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT1_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pPB-Neo-COVGT2-d2EGFP-mCherry
Plasmid#201452PurposeFluorescent reporter for genetic tracing of epithelial cell SARS-CoV2 response.DepositorInsertCOVGT2_d2EGFP-mCherry
UseSynthetic BiologyAvailable SinceJuly 17, 2023AvailabilityAcademic Institutions and Nonprofits only