We narrowed to 13,723 results for: cas9 genes

1. CMV-SpyCas9

   Plasmid

   #113033

   Purpose

   SpyCas9 expression in mammalian cells (CMV promoter driven)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   SpyCas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pT3TS-nCas9n

   Plasmid

   #46757

   Depositor

   Wenbiao Chen

   Article

   Jao et al Proc Natl Acad Sci U S A. 2013 Aug 5.

   Insert

   Cas9
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Bacterial

   Mutation

   Promoter

   T3

   Available since

   Aug. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGH125_dCas9-Blast

   Plasmid

   #85417

   Purpose

   lentiviral expression vector for dCas9 with Blasticidin selectable marker

   Depositor

   Michael Bassik

   Article

   Hess et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   dCas9 and Blasticidin resistance
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLentiCas9-BFP

   Plasmid

   #78545

   Purpose

   Fusion protein Cas9+BFP

   Depositor

   Roderic Guigo, Rory Johnson

   Article

   Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

   Insert

   BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Fusion protein with Cas9

   Available since

   July 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCas9-polyA

   Plasmid

   #72602

   Purpose

   express hCas9 byT7 promoter with polyadenine tails

   Depositor

   Tomoji Mashimo

   Article

   Yoshimi et al Nat Commun. 2016 Jan 20;7:10431. doi: 10

   Insert

   Cas9-polyadenine
   

   Use

   Tags

   81-bp polyadenylation

   Expression

   Mutation

   Promoter

   T7

   Available since

   July 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSpCas9 (PX165)

   Plasmid

   #48137

   Purpose

   Human codon optimized Cas9 nuclease from Streptococcus pyogenes (Cbh-3X-FLAG-NLS-SpCas9-NLS)

   Depositor

   Feng Zhang

   Article

   Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. SpCas9-mSA*

   Plasmid

   #182952

   Purpose

   Express SpCas9-mSA*

   Depositor

   Wen Xue

   Article

   Liang et al Nat Commun. 2022 Jan 21;13(1):437. doi:

   Insert

   SpCas9-mSA*
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. SpCas9 TadDE

   Plasmid

   #193837

   Purpose

   Express TadDE (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadA-DualEditor-Cas9D10A-2xUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. SpCas9-CBE6c

   Plasmid

   #215821

   Purpose

   Express CBE6c (with SpCas9) in mammalian cells

   Depositor

   David Liu

   Article

   Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

   Insert

   CBE6c-SpCas9-2xUGI (cas9 )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCRISPomyces-SaCas9

    Plasmid

    #129553

    Purpose

    Contains codon-optimized SaCas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    SaCas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYLCRISPR/Cas9P35S-N

    Plasmid

    #66191

    Purpose

    expression of Cas9 in plants, cauliflower mosaic virus 35S promoter (P35S), Neo selection

    Depositor

    Yao-Guang Liu

    Article

    Ma et al Mol Plant. 2015 Apr 24. pii: S1674-2052(

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Plant

    Mutation

    plant-codon optimized with higher GC contents (62…

    Promoter

    cauliflower mosaic virus 35S promoter (P35S)

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLentiCas9-GFP

    Plasmid

    #78546

    Purpose

    Fusion protein Cas9+GFP

    Depositor

    Roderic Guigo, Rory Johnson

    Article

    Pulido-Quetglas et al PLoS Comput Biol. 2017 Mar 2;13(3):e1005

    Insert

    GFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Fusion protein with Cas9

    Available since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pK7WGF2::hCas9

    Plasmid

    #46965

    Purpose

    Expesses the human codon usage Cas9 nuclease (Mali et al. Science 339, 823-826, 2013) with an N-terminal GFP tag from the 35S promoter in the plant tissue

    Depositor

    Sophien Kamoun

    Article

    Nekrasov et al Nat Biotechnol. 2013 Aug 8;31(8):691-3.

    Insert

    hCas9
    

    Use

    CRISPR; Plant expression

    Tags

    eGFP

    Expression

    Mutation

    Promoter

    35S

    Available since

    Aug. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pH-nCas9-PBE

    Plasmid

    #98163

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. gGBEv6.3-SpCas9

    Plasmid

    #202629

    Purpose

    vector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoter

    Depositor

    Huawei Tong

    Article

    Tong et al Natl Sci Rev. 2023 May 16;10(8):nwad143.

    Insert

    nSpCas9(D10A)-MPGv6.3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    MPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…

    Promoter

    hU6, EF1a, CBh

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCRISPomyces-Sth1Cas9

    Plasmid

    #129552

    Purpose

    Contains codon-optimized Sth1Cas9 and gRNA for streptomyces

    Depositor

    Mingzi M. Zhang, Fong Tian Wong

    Article

    Yeo et al Biotechnol Bioeng. 2019 May 15. doi: 10.

    Insert

    Sth1Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rpsL(XC)-BbsI

    Available since

    Oct. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. Topo_SpCas9.tracr_AsCas12a.DR

    Plasmid

    #155050

    Purpose

    TOPO vector for the cloning of _the SpCas9 tracrRNA - AsCas12a Direct Repeat (DR) fragment into the pLCHKO hgRNA vector

    Depositor

    Jason Moffat, Benjamin Blencowe

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    SpCas9 tracrRNA and AsCas12a Direct Repeat (DR)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCS2+ heiCas9

    Plasmid

    #186929

    Purpose

    For in vitro transcription of heiCas9 mRNA - mammalian codon optimized Cas9, N-terminal hei-tag (Myc-flexible linker-optimizedNLS) and C-terminal oNLS

    Depositor

    Joachim Wittbrodt

    Article

    Thumberger et al Elife. 2022 Mar 25;11. pii: 70558. doi:

    Insert

    myc-flexible linker-oNLS-Cas9-oNLS
    

    Use

    CRISPR

    Tags

    myc-flexible linker-optimizedNLS and optimizedNLS

    Expression

    Mammalian

    Mutation

    Promoter

    sp6

    Available since

    April 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAG-hCas9

    Plasmid

    #51142

    Purpose

    Expresses hCas9 under the CAG promoter for CRISPR

    Depositor

    Izuho Hatada

    Article

    Horii et al Int J Mol Sci. 2013 Sep 30;14(10):19774-

    Insert

    hCas9
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Feb. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

20. pLKO5d.SFFV.Cas9.P2A.BSD

    Plasmid

    #181981

    Purpose

    Lentiviral Cas9 expression

    Depositor

    Jan-Henning Klusmann

    Insert

    Cas9 (cas9 Streptococcus pyogenes (strain: NGAS638))
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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