We narrowed to 17,589 results for: She

1. MSCV-mouse p19ARF-IRES-GFP

   Plasmid

   #107147

   Purpose

   Mammalian expression of mouse Arf

   Depositor

   Martine Roussel

   Article

   Kuo et al Cancer Res. 2003 Mar 1;63(5):1046-53.

   Insert

   Arf [alternative reading frame] (Cdkn2a Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   April 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pME V5-mTagBFP2 (JDW 830)

   Plasmid

   #224484

   Purpose

   Gateway compatible middle entry clone containing V5 tagged mTagBFP2 (Cytosolic blue fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   V5-mTagBFP2
   

   Use

   Gateway cloning

   Available Since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. hP2X3-cALFA

   Plasmid

   #160499

   Purpose

   For the expression of human P2X3 with ALFA tag at C-terminus in mammalian cells

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Insert

   human P2X3 (P2RX3 Synthetic, Human)
   

   Tags

   HRV3C protease cleavage site-ALFA tag-twin Strep …

   Expression

   Mammalian

   Mutation

   ΔN5ΔC33-T13P/S15V/V16I

   Available Since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSR357.24

   Plasmid

   #125109

   Purpose

   Expression of narL(1-159aa)-ydfI(153-213aa) chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-ydfI(DBD)159
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Available Since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. FLAG-ARP8

   Plasmid

   #15361

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Jin et al J Biol Chem. 2005 Dec 16. 280(50):41207-

   Insert

   actin-related protein 8 (ACTR8 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   Aug. 17, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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6. Diacylglycerol Kinase Zeta

   Plasmid

   #85454

   Purpose

   expression of Diacylglycerol Kinase Zeta

   Depositor

   Stephen Gee

   Article

   Hogan et al J Biol Chem. 2001 Jul 13;276(28):26526-3

   Insert

   Diacylglycerol Kinase Zeta (DGKZ Human)
   

   Tags

   3x HA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. PCRT7 tetR pLtetO MBP-MEK1 XX Amp

   Plasmid

   #53225

   Purpose

   human MEK1 with MBP and HIS tags, with Ser218 and Ser222 changed to amber codons, for use in phosphoprotein synthesis

   Depositor

   Jesse Rinehart

   Insert

   MEK1 (MAP2K1 Human)
   

   Use

   Phosphoprotein synthesis

   Tags

   HIS and MBP

   Expression

   Bacterial

   Mutation

   Codon optimized for E.coli, both codons for Ser21…

   Promoter

   pLtetO

   Available Since

   May 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. lentiMutB3GALT5catdead-blast

   Plasmid

   #220869

   Purpose

   lentiviral vector for expressing human B3GALT5 with D156A and D243A inactivating mutations and C-terminal myc-DDK tag, includes nucleotide change in PAM targeting sequence

   Depositor

   Jennifer Kohler

   Insert

   beta-1,3-galactosyltransferase 5 (B3GALT5 Human)
   

   Use

   Lentiviral

   Tags

   myc, FLAG

   Expression

   Mammalian

   Mutation

   encodes D156A and D243A mutations; also, nucleoti…

   Available Since

   Sept. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-puro-tetON-shGFP

   Plasmid

   #110939

   Purpose

   Tet-inducible shRNA targeting GFP

   Depositor

   William Hahn

   Article

   Ilic et al Proc Natl Acad Sci U S A. 2017 Apr 25;11

   Insert

   shGFP
   

   Use

   Lentiviral and RNAi

   Promoter

   H1/TO

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBAD-SPW-RHH

    Plasmid

    #190630

    Purpose

    Bacterial expression of genetically encoded protease indicator SPW-RHH

    Depositor

    Robert Campbell

    Article

    Gohil et al ACS Sens. 2023 Feb 24;8(2):587-597. doi:

    Insert

    SPW-RHH
    

    Expression

    Bacterial

    Available Since

    Nov. 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUBC_HA_PP7-mVenus

    Plasmid

    #111268

    Purpose

    Transient expression of non-aggregating delta-FG mutant, fused to mVenus in mammalian cells, under a hUBC promoter, HA tag

    Depositor

    John Rinn

    Article

    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10

    Insert

    PP7-mVenus
    

    Use

    Synthetic Biology

    Tags

    HA, SV40 NLS, and mVenus

    Expression

    Mammalian

    Mutation

    PP7: C68S, S69G [delta]70-75 (non-aggregating)

    Promoter

    hUBC

    Available Since

    June 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. PX458_3XHA_den1FnCas9_KRAB_T2A_eGFP

    Plasmid

    #200910

    Purpose

    3XHA tagged dead en1Cas9 from F. novicida with KRAB repressor domain, T2A self-cleaving peptide and enhanced GFP biomarker for mammalian expression

    Depositor

    Debojyoti Chakraborty

    Insert

    3XHA_den1FnCas9_KRAB_eGFP
    

    Use

    CRISPR

    Tags

    3xHA-SV40NLS and Nucleoplasmin NLS-KRAB-T2A-EGFP

    Expression

    Mammalian

    Mutation

    Changed Glutamic Acid to Arginine at 1369 position

    Promoter

    CBH

    Available Since

    Sept. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. CYFIP2.mCherry

    Plasmid

    #122052

    Purpose

    Expresses human CYFIP2 in mammalian cells.

    Depositor

    Josef Kittler

    Article

    Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

    Insert

    CYFIP2 (CYFIP2 Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. p5E-hs_ICAM_full (JDW 1047)

    Plasmid

    #224479

    Purpose

    Gateway compatible 5' entry clone containing the Human ICAM2 promoter

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    ICAM2 promoter
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV_eA3A-nCas9

    Plasmid

    #163535

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    eA3A-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Available Since

    Dec. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5-Flag CASTOR1 I280A

    Plasmid

    #84495

    Purpose

    overexpression of CASTOR1 I280A mutant

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR1 I280A (GATSL3 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I280A

    Promoter

    CMV

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pLHCX-Flag-mPKM2(C358S)

    Plasmid

    #42513

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 Cys358->Ser
    

    Use

    Retroviral

    Tags

    Flag

    Mutation

    Changed Cysteine 358 to Serine

    Promoter

    CMV

    Available Since

    March 19, 2013

    Availability

    Academic Institutions and Nonprofits only

18. pPV-H1-ccdB-mEF1α-RiH

    Plasmid

    #100598

    Purpose

    piggyBac vector for sgRNA cloning

    Depositor

    Akitsu Hotta

    Article

    Ishida et al Sci Rep. 2018 Jan 10;8(1):310. doi: 10.1

    Type

    Empty backbone

    Use

    Piggybac vector

    Expression

    Mammalian

    Promoter

    Human H1 PolIII promoter, human FTH1 promoter, mo…

    Available Since

    April 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti-CaMKIIa-eArch 3.0-EYFP

    Plasmid

    #35514

    Purpose

    Lentiviral expression of CaMKIIa-driven Arch 3.0 for optical inhibition.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    eArch3.0-EYFP
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Enhanced trafficking signal and ER export signal …

    Promoter

    CaMKIIa

    Available Since

    May 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. LP455

    Plasmid

    #90198

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 649-1426

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 649-1426

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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21. MSCV-human CSFI-R-L301S-IRES-GFP

    Plasmid

    #133744

    Purpose

    retroviral expression of CSF1-R mutant

    Depositor

    Martine Roussel

    Article

    Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

    Insert

    CSF1-R (CSF1R Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    L301S

    Promoter

    MSCV

    Available Since

    Dec. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. CDK4-luciferase full length (2135)

    Plasmid

    #86656

    Purpose

    Luciferase reporter containing CDK4 promoter

    Depositor

    Jaime Modiano

    Article

    Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

    Insert

    CDK4 promoter (CDK4 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    2120 base pairs including up to coding nucleotide…

    Promoter

    CDK4

    Available Since

    June 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. LP453

    Plasmid

    #90184

    Purpose

    pFLAG-CMV2 with cDNA encoding HsNPHP4 aa residues 1-839

    Depositor

    Lotte Pedersen

    Article

    Schou et al Nat Commun. 2017 Jan 30;8:14177. doi: 10

    Insert

    NPHP4 (NPHP4 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    aa residues 1-839

    Available Since

    May 16, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

    Plasmid

    #133779

    Purpose

    Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    Isocitrate dehydrogenase 1 (IDH1 Human)
    

    Tags

    Myc and FLAG

    Expression

    Mammalian

    Mutation

    Arginine is mutated to histidine at amino acid po…

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pTrc99S-ssDsbA-EPA-DNNNS-DQNRT

    Plasmid

    #128404

    Purpose

    Periplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Exotoxin A (toxA P. aeruginosa)
    

    Tags

    6xHis tag and DsbA signal sequence for periplasmi…

    Expression

    Bacterial

    Mutation

    242DNNNS246 and 384DQNRT388 engineered glycosylat…

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAAV-CAG-SuperNovaGreen-T2A-dTomato

    Plasmid

    #193798

    Purpose

    Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

    Depositor

    Xiao Wang

    Article

    Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

    Insert

    SuperNova Green
    

    Use

    AAV

    Tags

    Flag tag

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

    Plasmid

    #123360

    Purpose

    Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

    Depositor

    Sidi Chen

    Article

    Chow et al Nat Methods. 2019 May;16(5):405-408. doi

    Insert

    Empty LbCpf1 crRNA cloning cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

29. mCardinal-Paxillin-22

    Plasmid

    #56171

    Purpose

    Localization: Focal Adhesions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Paxillin
    

    Tags

    mCardinal

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. p3E-V5-mScarlet-I_SV40pA (JDW 968)

    Plasmid

    #224529

    Purpose

    A Gateway compatible 3' entry clone containing an V5 mScarlet fusion followed by SV40 late polyA

    Depositor

    Joshua Wythe

    Article

    Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

    Insert

    V5-mScarlet-I
    

    Use

    Gateway cloning

    Available Since

    Sept. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pUASp YFP Ras85D WT

    Plasmid

    #52242

    Purpose

    Drosophila expression of YFP-Ras85D

    Depositor

    Matthew Scott

    Insert

    Ras85D (Ras85D Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Promoter

    GAL4

    Available Since

    March 20, 2014

    Availability

    Academic Institutions and Nonprofits only

32. (326) pAAV SV40-ZsGreen U6-gRNA

    Plasmid

    #163020

    Purpose

    Fluorescent reporter for Sa gRNA (Bsa1 sites)

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Zs Green
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pSR357.10

    Plasmid

    #125106

    Purpose

    Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

    Depositor

    Jeffrey Tabor

    Article

    Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

    Inserts

    narL(REC)-ydfI(DBD)131
    sfgfp
    tetR
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    June 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pTrc99S-ssDsbA-TTc-4xDQNAT

    Plasmid

    #128401

    Purpose

    Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin fragment C domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pTrc99S-ssDsbA-TTlight-4xDQNAT

    Plasmid

    #128402

    Purpose

    Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

    Depositor

    Michael Jewett

    Article

    Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

    Insert

    Tetanus toxin light chain domain
    

    Tags

    4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

    Expression

    Bacterial

    Mutation

    Inactivating E234A mutation

    Promoter

    trc

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBV-luc TOP2A 1kb promoter

    Plasmid

    #138274

    Purpose

    Luciferase reporter plasmid driven by TOP2A promoter

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A promoter sequence
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pRK5 CASTOR2-Flag Cterm

    Plasmid

    #84490

    Purpose

    overexpression of CASTOR2 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

38. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available Since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pET28a-His-hPKM2

    Plasmid

    #42515

    Depositor

    Dimitrios Anastasiou

    Article

    Anastasiou et al Science. 2011 Dec 2;334(6060):1278-83. d

    Insert

    Pyruvate Kinase M2 (PKM Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    March 7, 2013

    Availability

    Academic Institutions and Nonprofits only

40. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Promoter

    T7

    Available Since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge