We narrowed to 22,837 results for: Sis

1. pHH0103_TNK2-1/1

   Plasmid

   #91377

   Purpose

   Protein expression and purification of human SH3 domain construct TNK2-1/1

   Depositor

   Sachdev Sidhu

   Article

   Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

   Insert

   TNK2-1/1 (TNK2 Human)
   

   Expression

   Bacterial

   Available Since

   March 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-globin_del5UTR_WT-xrRNA-4H

   Plasmid

   #108366

   Purpose

   Expresses wild type beta-globin reporter; enables detection of 5'-3' decay intermediates (xrFrag) and allows detection via northern blot (4H binding sites)

   Depositor

   Niels Gehring

   Article

   Boehm et al Nat Commun. 2016 Dec 5;7:13691. doi: 10.

   Insert

   Wild type beta-globin reporter with MVE xrRNA and 4H probe binding sites
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1-dCas9-CibN

   Plasmid

   #60552

   Purpose

   Expresses dCas9-CibN in mammalian cells

   Depositor

   Charles Gersbach

   Article

   Polstein et al Nat Chem Biol. 2015 Feb 9. doi: 10.1038/

   Insert

   dCas9-CibN
   

   Use

   CRISPR

   Tags

   FLAG and SV40 NLS

   Expression

   Mammalian

   Mutation

   inactivating Cas9 mutations D10A and H840A

   Promoter

   CMV

   Available Since

   Jan. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJMP1363

   Plasmid

   #119279

   Purpose

   for stabilizing ICE in the presence of rapI expression

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Jan. 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSpCas9(BB)-2A-tomato-TSC2-sgRNA

   Plasmid

   #196195

   Purpose

   Editing human TSC2 locus

   Depositor

   Jürgen Knoblich

   Article

   Sidhaye et al Elife. 2023 Mar 29;12:e85135. doi: 10.75

   Insert

   TSC2 (TSC2 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJMP1104

   Plasmid

   #119248

   Purpose

   knockdown pqsC in P. aeruginosa

   Depositor

   Carol Gross, Jason Peters, Oren Rosenberg

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   sgRNA pqsC (P. aeruginosa)
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   Feb. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. px330-UFL1 sgRNA2

   Plasmid

   #134636

   Purpose

   contains sgRNA targeting human UFL1 for gene knockout

   Depositor

   Yihong Ye

   Article

   Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

   Insert

   UFL1 sgRNA2 (UFL1 Human)
   

   Expression

   Mammalian

   Available Since

   Nov. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. mRIP3 GFP D161N

   Plasmid

   #41383

   Depositor

   Francis Chan

   Article

   Cho et al Cell. 2009 Jun 12;137(6):1112-23.

   Insert

   RIP3 (Ripk3 Mouse)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Kinase Domain Mutant (D161N)

   Promoter

   CMV

   Available Since

   Dec. 4, 2012

   Availability

   Academic Institutions and Nonprofits only

9. pCGN-ELK-1 Ets mut

   Plasmid

   #27157

   Purpose

   For mammalian expression of human Elk-1 with an HA tag and a mutation in the Ets DNA binding domain

   Depositor

   Ron Prywes

   Article

   Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

   Insert

   ELK1 (ELK1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   R62E/R65E/Y66D

   Available Since

   Dec. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pCDF-NtXNtS1R1AtR2NtB2

    Plasmid

    #160881

    Purpose

    Expression of N. tabacum Rubisco small subunit (rbcS-S1), Rubisco chaperone protiens rbcX, raf1, bsd2 and A. thaliana Rubisco chaperone proteins raf2 in E. coli.

    Depositor

    Maureen Hanson

    Article

    Lin et al Nat Plants. 2020 Oct;6(10):1289-1299. do

    Insert

    rbcS-S1, rbcX, raf1, raf2, bsd2
    

    Expression

    Bacterial

    Promoter

    rbcS-S1: T7, chaperones: T7

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pT2SCb

    Plasmid

    #59385

    Purpose

    Template plasmid for amplification of a selection cassette that includes two transcription terminator elements, an I-SceI site and a modified chloramphenicol resistance gene.

    Depositor

    Shelley Copley

    Article

    Kim et al BMC Biotechnol. 2014 Sep 25;14(1):84.

    Insert

    TT-ISceI-cat2
    

    Use

    Template

    Mutation

    The 3’-end of the chloramphenicol resistance gene…

    Available Since

    Sept. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. peCALWY-2

    Plasmid

    #22234

    Depositor

    Maarten Merkx

    Article

    Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

    Insert

    eCALWY-2 Zn(II) sensor (ATOX1 Human, GFP's)
    

    Expression

    Mammalian

    Mutation

    sequence identical to eCALWY-1, but linkerlength …

    Available Since

    Nov. 20, 2009

    Availability

    Academic Institutions and Nonprofits only

13. pBS SK mCherryROSAbsr

    Plasmid

    #54321

    Purpose

    Encodes mCherry fused with out-of-frame blasticidin-S resistance gene with a linker containing a target for TALEN/CRISPR. Can use as a surrogate target to select cells expressing active TALEN/CRISPR.

    Depositor

    Silvestro Conticello

    Article

    Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

    Insert

    mCherry and BlasticidinS-resistance
    

    Use

    CRISPR, Mouse Targeting, and TALEN

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGWB421

    Plasmid

    #74815

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with 10xMyc (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSAG1::CAS9-U6::sg290860-6

    Plasmid

    #59855

    Purpose

    Encodes CAS9 nuclease (GFP fusion) under control of the Toxoplasma gondii SAG1 promotor. Vector also provides U6 controlled expression of a single guide RNA for CRISPR disruption of TGME49_290860.

    Depositor

    David Sibley

    Article

    Behnke et al Eukaryot Cell. 2014 Dec 5. pii: EC.00229

    Insert

    CRISPR sg290860-6
    

    Use

    CRISPR; ; toxoplasma gondii

    Available Since

    Oct. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. 3540 pMIG Bid p15

    Plasmid

    #8789

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

17. pBABE-Src-Rescue

    Plasmid

    #26983

    Depositor

    Joan Massague

    Article

    Zhang et al Cancer Cell. 2009 Jul 7. 16(1):67-78.

    Insert

    c-Src (CSK Chicken)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Dec. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

18. p168

    Plasmid

    #62618

    Purpose

    ePathBrick pETM6 vector encoding CsF3H, FaDFR, and DuLAR

    Depositor

    Mattheos Koffas

    Article

    Zhao et al Metab Eng. 2014 Dec 17;28C:43-53. doi: 1

    Inserts

    flavaone 3B-hydroxylase
    dihydroflavonol 4-reductase
    leucoanthocyanidin reductase
    

    Use

    Synthetic Biology

    Tags

    GBD, PDZ, and SH3

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. EcPPK1

    Plasmid

    #108850

    Purpose

    Expresses E. coli PPK1 in mammalian cell lines

    Depositor

    Michael Downey

    Article

    Bentley-DeSousa et al Cell Rep. 2018 Mar 27;22(13):3427-3439.

    Insert

    Polyphosphate Kinase 1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAdSh.U6.gRNAS1

    Plasmid

    #58252

    Purpose

    U6 promoter-driven single guide RNA complementary to the human AAVS1 locus

    Depositor

    Manuel Goncalves

    Article

    Maggio et al Sci Rep. 2014 May 29;4:5105. doi: 10.103

    Insert

    U6.gRNAS1 cassette
    

    Use

    Adenoviral and CRISPR

    Expression

    Mammalian

    Available Since

    Sept. 5, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. p6898 PHAGE-P CMVT N-HA-hE6AP I C820A

    Plasmid

    #37602

    Depositor

    Peter Howley

    Article

    Martinez-Noel et al Mol Cell Biol. 2012 May 29.

    Insert

    E6AP I C820A (UBE3A Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C820A

    Promoter

    CMV tet regulatable

    Available Since

    Aug. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

22. pARF1-YFP

    Plasmid

    #11390

    Depositor

    Joel Swanson

    Article

    Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

    Insert

    ADP Ribosylation Factor 1 (ARF1 Human)
    

    Tags

    YFP

    Expression

    Mammalian

    Available Since

    May 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. px330-UFL1 sgRNA1

    Plasmid

    #134635

    Purpose

    contains sgRNA targeting human UFL1 for gene knockout

    Depositor

    Yihong Ye

    Article

    Wang et al Cell Res. 2020 Jan;30(1):5-20. doi: 10.1

    Insert

    UFL1 sgRNA1 (UFL1 Human)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pQUAST-shibire (ts1)

    Plasmid

    #24362

    Depositor

    Liqun Luo

    Article

    Potter et al Cell. 2010 Apr 30. 141(3):536-48.

    Insert

    Shibire (ts1) (shi Fly)
    

    Expression

    Insect

    Available Since

    May 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lyn-CFP-GAI(1-92)

    Plasmid

    #37311

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    CFP and Lyn

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

26. pCMV-myc-ASPM MTB

    Plasmid

    #27668

    Depositor

    Joseph Loturco

    Article

    Paramasivam et al Cell Cycle. 2007 Jul 1. 6(13):1605-12.

    Insert

    Abnormal spindle-like microcephaly associated (Aspm Rat)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Contains amino‑terminal putative microtubule bind…

    Available Since

    April 1, 2011

    Availability

    Academic Institutions and Nonprofits only

27. pcDNA-HA-hTRAF3 1-448

    Plasmid

    #44033

    Depositor

    Shao-Cong Sun

    Article

    Liao et al J Biol Chem. 2004 Jun 18;279(25):26243-5

    Insert

    TNF receptor-associated factor 3 (TRAF3 Human)
    

    Tags

    1xHA

    Expression

    Mammalian

    Mutation

    Contains amino acids 1-448; V274D and S393G

    Available Since

    April 4, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCI-ING1b

    Plasmid

    #79052

    Purpose

    expression of p33ING1b in mammalian cells

    Depositor

    Karl Riabowol

    Article

    Scott et al J Cell Sci. 2001 Oct;114(Pt 19):3455-62.

    Insert

    ING1 (p33 ING1Ab isoform) (ING1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

29. pMSCV_Mock

    Plasmid

    #223565

    Purpose

    The plasmid is expressed in mammalian cells as a control.

    Depositor

    Hyun Woo Park

    Article

    Shin et al Mol Cancer. 2023 Nov 6;22(1):177. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Aug. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pJMP1171

    Plasmid

    #119253

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    dcas9
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pJMP1170

    Plasmid

    #119252

    Purpose

    rfp "test" strain

    Depositor

    Carol Gross, Jason Peters, Oren Rosenberg

    Article

    Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

    Insert

    sgRNA NT1/RR1 (rfp)
    

    Expression

    Bacterial

    Mutation

    D10A and H840A

    Available Since

    Jan. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pAd-Track SIRT1 H355A

    Plasmid

    #8441

    Depositor

    Pere Puigserver

    Article

    Rodgers et al Nature 2005 Mar 3;434(7029):113-8.

    Insert

    SIRT1 (Sirt1 Mouse)
    

    Use

    Adenoviral

    Expression

    Mammalian

    Mutation

    Histidine 355 mutated to Alanine

    Available Since

    Dec. 4, 2007

    Availability

    Academic Institutions and Nonprofits only

33. pICE-EGFP-FLAG-Ku70siR-WT

    Plasmid

    #46961

    Purpose

    Plasmid for constitutive or doxycycline-inducible expression of wild-type human Ku70 resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

    Depositor

    Steve Jackson

    Article

    Britton et al J Cell Biol. 2013 Jul 29.

    Insert

    human Ku70 (XRCC6 Human)
    

    Tags

    GFP-FLAG

    Expression

    Mammalian

    Promoter

    CMV Tet

    Available Since

    Aug. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pAd GFP HDAC5 WT

    Plasmid

    #32217

    Depositor

    Reuben Shaw

    Article

    Mihaylova et al Cell. 2011 May 13;145(4):607-21.

    Insert

    HDAC5 (HDAC5 Human)
    

    Use

    Adenoviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Oct. 5, 2011

    Availability

    Academic Institutions and Nonprofits only

35. pPSP6+t

    Plasmid

    #48149

    Purpose

    Shuttle vector E. coli/B.meg., encoding a promoter and a terminator for RNA polymerase of the phage SP6

    Depositor

    Dieter Jahn

    Article

    Stammen et al Appl Microbiol Biotechnol. 2010 Sep;88(2

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Bacterial

    Available Since

    Oct. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGWB524

    Plasmid

    #74866

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with GST (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Nov. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. 3538 pMIG Bad

    Plasmid

    #8787

    Depositor

    Stanley Korsmeyer

    Article

    Cheng et al Mol Cell 2001 Sep;8(3):705-11.

    Insert

    Bad (Bad Mouse)
    

    Use

    Retroviral

    Expression

    Mammalian

    Available Since

    Oct. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

38. pGoldyTALEN-VIM-TAL-2

    Plasmid

    #62852

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGoldyTALEN-VIM-TAL-1

    Plasmid

    #62855

    Purpose

    TALEN mediated gene editing at human VIM locus

    Depositor

    Gaudenz Danuser

    Article

    Gan et al Cell Syst. 2016 Sep 21. pii: S2405-4712(

    Insert

    TALEN array
    

    Use

    TALEN

    Expression

    Mammalian

    Promoter

    miniCAGG

    Available Since

    May 26, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. p413TEF-SirT1

    Plasmid

    #51746

    Purpose

    overexpression of human SirT1 in S. cerevisiae, HIS3 marker

    Depositor

    Markus Ralser

    Article

    Ralser et al PLoS One. 2009;4(4):e5376. doi: 10.1371/

    Insert

    SirT1 (SIRT1 Human)
    

    Expression

    Yeast

    Promoter

    TEF1

    Available Since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge