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We narrowed to 18,759 results for: nls

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Showing: 10761 - 10800 of 18759 results

  5. 6xHis-MBP-huMb3Cas12a

    Plasmid
    #174677
    Purpose
    Expresses 6xHis-MBP tagged Mb3Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huMb3Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174677-plasmid-map-sequence-id-346166

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  6. 6xHis-MBP-huPb2Cas12a

    Plasmid
    #174686
    Purpose
    Expresses 6xHis-MBP tagged Pb2Cas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huPb2Cas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174686-plasmid-map-sequence-id-346201

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  7. 6xHis-MBP-huHkCas12a

    Plasmid
    #174683
    Purpose
    Expresses 6xHis-MBP tagged HkCas12a
    Depositor
    Piyush Jain
    Publication
    Nguyen et al medRxiv 2021.07.21
    Insert
    huHkCas12a
    Use
    CRISPR and Synthetic Biology
    Tags
    6xHis, MBP, TEV, NLS and NLS, 6xHis, 3xHA
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Oct. 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    174683-plasmid-map-sequence-id-346190

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  9. pTUB1-Spy.dCas9-CAT-U6-sgRNA(decoy)

    Plasmid
    #171089
    Purpose
    CRISPR interference. Construct for expressing catalytically inactive Cas9 (dCas9) from Streptococcus pyogenes in Toxoplasma gondii. Suitable for generating stable cell lines.
    Depositor
    Sebastian Lourido
    Publication
    Markus et al mSphere. 2021 Oct 13:e0047421. doi: 10.1
    Inserts
    Decoy sgRNA
    dCas9
    Chloramphenicol acetyltransferase
    Use
    CRISPR; Toxoplasma gondii expression
    Tags
    3X FLAG and Nuclear Localization Signal
    Mutation
    D10A, H840A
    Promoter
    TUB1 (Toxoplasma gondii) and U6 (Toxoplasma gondi…
    Available since
    Oct. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    171089-plasmid-map-sequence-id-339962

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  19. PEA1-Nuclease-Puro HEK3 CTT ins

    Plasmid
    #171995
    Purpose
    Delivers all prime editing nuclease components targeting the HEK3 gene for a CTT inserrtion, in a single, puromycin selectable plasmid
    Depositor
    Paul Thomas
    Publication
    Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HEK3 CTT insertion pegRNA and CbH-Cas9-RT-T2A-Puro, hU6-pegRNA HEK3 CTT ins, hU6-sgRNA sham
    Expression
    Mammalian
    Promoter
    CMV for Cas9, U6 for gRNAs
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    171995-plasmid-map-sequence-id-341857

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  23. pX330_CALR sgRNA / hSpCas9

    Plasmid
    #172838
    Purpose
    Mammalian expression of a sgRNA targeting the intron 1 of CALR (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.
    Depositor
    Haining Zhong
    Publication
    Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    sgRNA targeting intron 1 of CALR under the U6 promotor and hSpCas9 under the CAG promotor
    Use
    CRISPR
    Expression
    Mammalian
    Available since
    Aug. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    172838-plasmid-map-sequence-id-340912

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  26. pIVTR(T7A)-CRE-T2A-PuroR

    Plasmid
    #172298
    Purpose
    In vitro transcription of CRE-T2A-PuroR synthetic construct for the excision of floxed cassettes
    Depositor
    Patrik Verstreken
    Insert
    CRE-T2A-PuroR
    Use
    Cre/Lox
    Promoter
    T7 promoter A-initiating
    Available since
    Aug. 19, 2021
    Availability
    Academic Institutions and Nonprofits only
    172298-plasmid-map-sequence-id-340323

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  33. pX458-Ef1a-Cas9-H2B-mCherry (dual gRNA: Olig2 x2)

    Plasmid
    #171101
    Purpose
    CRISPR/Cas9 expressing plasmid containing two gRNAs targeting mouse Olig2.
    Depositor
    Joseph Brzezinski
    Publication
    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hSpCas9 (Olig2 Mouse, S. pyogenes)
    Use
    CRISPR
    Promoter
    Ef1a
    Available since
    June 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    171101-plasmid-map-sequence-id-336130

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  34. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid
    #169914
    Purpose
    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.
    Depositor
    Zhili Rong
    Publication
    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CjCas9
    Use
    CRISPR
    Tags
    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 25, 2021
    Availability
    Academic Institutions and Nonprofits only
    169914-plasmid-map-sequence-id-336053

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  37. pW210-pPB-GA-inducible-CRISPRa-sa_dCas9-VPR-hygR

    Plasmid
    #170808
    Purpose
    PiggyBac vector to express GA-inducible VPR-Sa_dCas9 with hygromycin resistance
    Depositor
    Kenneth Yamada
    Publication
    Wang et al Cell. 2021 Jul 8;184(14):3702-3716.e30.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    GID1-VPR-IRES-HygR
    GAI-tagBFP-Sa_dCas9 (GAI Mustard Weed, Synthetic, S. aureus)
    Expression
    Mammalian
    Mutation
    Synonymous mutation in PYL1 to remove XhoI site (…
    Available since
    June 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    170808-plasmid-map-sequence-id-334406

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  38. SpyCas9 PE2-3HA

    Plasmid
    #169853
    Purpose
    Mammalian Expression, SpyCas9 prime editor with 3HA-tag
    Depositor
    Scot Wolfe
    Publication
    Liu et al Nat Commun. 2021 Apr 9;12(1):2121. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SpCas9-H840A-M-MLV-3HA
    Tags
    3XHA tag and bpSV40 NLS
    Expression
    Mammalian
    Promoter
    cmv
    Available since
    June 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    169853-plasmid-map-sequence-id-332830

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  39. pMB952:PB-CAG-PuroR-nGFP-FLEx-SaCas9-P2A-mCherry-WPRE

    Plasmid
    #168107
    Purpose
    PiggyBac transposon vector constitutively driving PuroR-eGFP and conditionally expressing S.aureus Cas9 and mCherry
    Depositor
    Erich Jarvis
    Publication
    Biegler et al Sci Rep. 2022 Mar 14;12(1):4369. doi: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cas9-P2A-mCherry
    Use
    CRISPR and Cre/Lox
    Tags
    HA
    Promoter
    CAG
    Available since
    May 28, 2021
    Availability
    Academic Institutions and Nonprofits only
    168107-plasmid-map-sequence-id-329724

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Showing: 10761 - 10800 of 18759 results