We narrowed to 62,913 results for: cloning
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Plasmid#179120PurposeExpresses control sgRNAs under the U6 promoter and mcherry protein specifically in astrocytesDepositorInsertcontrol sgRNA
UseAAVPromoterU6Available sinceNov. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSLPB2B-FKBP12_F36V-hHDAC4-SpdCas9-tagBFP-PGK-Blasticidin
Plasmid#187956PurposeFKBP12 (F36V mutant) degron-tagged hHDAC4-dCas9 fused with tagBFP, expressed under EF1-alpha promoter in a piggyBac plasmid. Expresses Blasticidin resistance under PGK promoter.DepositorInsertFKBP12_F36V-hHDAC4-SpdCas9-tagBFP
UseCRISPR and Synthetic BiologyExpressionMammalianAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
EB3N-VVDfast-Halo-iLID
Plasmid#190166PurposeExpresses EB3N-VVDfast-Halo-iLID, a microtubule anchor used for optogenetic recruitment of SspB-mCh-p60, in mammalian cells.DepositorInsertEB3N-VVDfast-Halo-iLID (MAPRE3 Human, Synthetic)
Tags-VVDfast-mClover3-iLIDExpressionMammalianMutation1-189 aa from EB3Available sinceOct. 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
SpCas9 nickase for Split-PE (pJUL2497)
Plasmid#190104PurposeExpresses nickase SpCas9(H840A) for Split-PE useDepositorInsertnSpCas9
TagsXTEN-bpNLS and bpNLSExpressionMammalianMutationH840APromoterCMVAvailable sinceSept. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-3*Flag-APEX2 C-term 3NLS
Plasmid#187588PurposeExpress FLAG epitope and APEX2-tagged 3xNLS fusion protein in mammalian cellsDepositorInsertNLS
TagsAPEX2-FLAGExpressionMammalianPromoterCMVAvailable sinceJuly 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
Geminin-mStrawberry reporter
Plasmid#158690PurposeThis plasmid is a Geminin cell cycle reporter. It has CMV promoter driving the expression of Geminin fused to mStrawberry-pGK-BSDDepositorInsertmStrawberry
UseLentiviralExpressionMammalianPromoterCMVAvailable sinceMay 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBHM2329
Plasmid#171686PurposepRS316-PCnU6-scaffold, contains template for amplification of PCnU6 and scaffold for fusion PCRDepositorInsertPCnU6-sgRNA_scaffold
UseCRISPRExpressionBacterial and YeastPromoterC. neoformans U6Available sinceMay 4, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pOT_2 - lenti-EFS-tNGFR-2A-puro
Plasmid#181971PurposeExpresses human truncated NGFR linked to puromycin resistance via P2A for lentiviral delivery.DepositorAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TALED_Right-ND1-1397N
Plasmid#183898PurposeExpresses TALED_Right-ND1-1397N in mammalian cellsDepositorInsertTALED for ND1 mutation
ExpressionMammalianPromoterpCMVAvailable sinceApril 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-puroeGFP
Plasmid#177922PurposeConstitutively expresses eGFP with puromycin selectionDepositorInserteGFP
ExpressionMammalianPromoterCMVAvailable sinceApril 12, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pDonor14-ShHELIX-KanR (CJT70)
Plasmid#181793PurposepDonor for ShHELIX containing 14bp of spacing between the I-AniI site and LE/RE and encoding a kanamycin resistance gene as cargoDepositorInsertKanR, R6K origin of replication
ExpressionBacterialAvailable sinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Nluc
Plasmid#180501PurposeMammalian expression of NlucDepositorPublicationInsertNluc
TagsFLAGExpressionMammalianPromoterCMVAvailable sinceMarch 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO_H2B-HaloTag7-T2A-Tom20-HaloTag9
Plasmid#175547PurposeCo-expression of HaloTag7 (nuclear) and 9 (mitochondrial surface) in mammalian cellsDepositorInsertH2B-HaloTag7 and Tomm20-HaloTag9
ExpressionMammalianMutationHaloTag9 = HaloTag7-Q165H-P174RAvailable sinceDec. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCE35
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRExpressionBacterialAvailable sinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable sinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)
Plasmid#174515PurposeRecoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)DepositorInsertsMm PylS
MmtRNA-Pyl-opt(UGA)
ExpressionBacterialMutationASL from the serT gene (UGA anticodon) and recodedPromoterGlnS and lppAvailable sinceOct. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCMV-SaPE2-P2A-BSD
Plasmid#174818PurposeMammalian expression of SaCas9 prime editor 2 with P2A-BSD markerDepositorInsertSaPE2-P2A-BSD
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscriptPromoterCMVAvailable sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDF0317 HvsCas7-11 mammalian expression
Plasmid#172511PurposeEncodes human codon optimized HvsCas7-11 protein alone for expression in mammalian cellsDepositorInsertHvsCas7-11 mammalian expression
ExpressionMammalianAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV.hSyn.FLEX.iGluSnFR3.v857.SGZ.codonopt
Plasmid#175182PurposeFluorescent reporter for glutamate, third generation, variant 857 in Stargazin (SGZ) backbone. iGluSnFR3.v857.SGZDepositorInsertpAAV hSyn FLEX iGluSnFR3 v857.SGZ
UseAAV, Cre/Lox, Mouse Targeting, and Synthetic Biol…TagsIgK-chain, Myc epi tag, and Stargazin and PDZ dom…ExpressionMammalianPromoterhuman SynapsinAvailable sinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSF278
Plasmid#167673PurposeIntermediate vector for three gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for GatewayDepositorInsertattL1-ccdB-attL2
UseCRISPRAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by