We narrowed to 59,446 results for: cloning

1. p667-UBC-ALPK2-V5-miniTurbo_IDG-K

   Plasmid

   #198009

   Purpose

   Gateway destination clone of ALPK2 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   ALPK2-V5-miniTurbo (ALPK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pSB700 lenti gRNA iRFP713_zhang2.0

   Plasmid

   #167916

   Purpose

   Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

   Depositor

   Alejandro Chavez, Nan Cher Yeo

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAGM53151

   Plasmid

   #169093

   Purpose

   Level 1 cloning vector for transient expression. Already contains the 35S promoter and the Nos terminator.

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Front Plant Sci. 2021 Jun 9;12:682443. d

   Type

   Empty backbone

   Use

   Moclo cloning vector

   Expression

   Plant

   Available Since

   Aug. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. GGE151

   Plasmid

   #120751

   Purpose

   Part of GoldenGate Yarrowia lipolytica toolkit for assembling an expression plasmid for Y. lipolytic yeast. Promoter for gene expression in position 2 (P2_4UASxpr-TEF)

   Depositor

   Tristan Rossignol

   Article

   Larroude et al Microb Biotechnol. 2019 May 31. doi: 10.

   Insert

   P2_4UASxpr-TEF
   

   Use

   Cloning/assembly plasmid

   Available Since

   Nov. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. p667-UBC-CDK12-V5-miniTurbo_IDG-K

   Plasmid

   #135243

   Purpose

   Gateway destination clone of CDK12 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   CDK12-V5-miniTurbo (CDK12 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-miniTurbo

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   April 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR221-kcnk3b

   Plasmid

   #208986

   Purpose

   Riboprobe generation

   Depositor

   GuangJun Zhang

   Article

   Park et al Dev Dyn. 2024 Jan 25. doi: 10.1002/dvdy.

   Insert

   kcnk3b (kcnk3b Zebrafish)
   

   Use

   Cloning

   Promoter

   T7

   Available Since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLNHA-C1-HsTAB182_K

   Plasmid

   #146774

   Purpose

   Mammalian Expression of HsTAB182

   Depositor

   Elisa Izaurralde

   Article

   Braun et al Mol Cell. 2011 Oct 7;44(1):120-33.

   Insert

   HsTAB182 (TNKS1BP1 Human)
   

   Expression

   Mammalian

   Mutation

   2 silent mutations compared to the sequence given…

   Available Since

   March 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. p667-UBC-BRSK2-V5-TurboID_IDG-K

   Plasmid

   #135253

   Purpose

   Gateway destination clone of BRSK2 (human) tagged with C-terminal V5-TurboID for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   BRSK2-V5-TurboID (BRSK2 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   V5-TurboID

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Jan. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAK008

   Plasmid

   #128225

   Purpose

   classic sgRNA backbone for tRNA-gRNA array, Position 2

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for tRNA-gRNA array, Position 2
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAK009

    Plasmid

    #128226

    Purpose

    classic sgRNA backbone for tRNA-gRNA array, Position 3

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    classic sgRNA backbone for tRNA-gRNA array, Position 3
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Plant

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRATIO4214

    Plasmid

    #141314

    Purpose

    Gateway cloning compatible dual fluorescent ratiometric binary vector

    Depositor

    David Nelson

    Article

    Khosla et al Plant Direct. 2020 Jun 21;4(6):e00231. d

    Type

    Empty backbone

    Tags

    mCerulean and mScarlet-I

    Expression

    Plant

    Promoter

    UBQ10 promoter

    Available Since

    Sept. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSB700 lenti gRNA EBFP2

    Plasmid

    #167905

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKOdeltagalK (Kan)

    Plasmid

    #110089

    Purpose

    Analogous to pKO but without the negative selection gene galK.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Use

    Bacterial knockout generation

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pGBW-m4092709

    Plasmid

    #153859

    Purpose

    Bacterial expression plasmid for fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR

    Depositor

    Ginkgo Bioworks, Benjie Chen

    Insert

    fragment of SARS-CoV-2 orf1ab 3pUTR to viral genome 3pUTR (ORF1ab Severe acute respiratory syndrome coronavirus 2)
    

    Expression

    Bacterial

    Mutation

    Nearly native encoding;handful SNPs as watermarks…

    Promoter

    Promoter | t7 consensus from NEB, G is +1

    Available Since

    June 12, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. p3E-2a-6MTnlstdTomato

    Plasmid

    #67699

    Purpose

    3' entry clone with 6xmyc nuclear localized tdTomato

    Depositor

    Nathan Lawson

    Article

    Shin et al Development. 2016 Oct 15;143(20):3785-37

    Insert

    tdTomato
    

    Tags

    2A-6xMyc-NLS

    Available Since

    Aug. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGKO2-Gateway

    Plasmid

    #63618

    Purpose

    a binary Agrobacterium vector carrying a modified HSVtk gene as a negative selection marker against ectopic transformants. Uses Gateway cloning.

    Depositor

    Seogchan Kang

    Article

    Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

    Type

    Empty backbone

    Use

    Fungal expression

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC18T-mini-Tn7T-Gm-REP

    Plasmid

    #64966

    Purpose

    mini-Tn7 vector with oriR6K, for attTn7 site determination

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Insert

    oriR6K
    

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. MXS_PGK::tTA2-bGHpA

    Plasmid

    #62448

    Purpose

    MXS_chaining vector with PGK::tTA2-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. MXS_PGK::rtTA3-bGHpA

    Plasmid

    #62446

    Purpose

    MXS_chaining vector with PGK::rtTA3-bGHpA

    Depositor

    Pierre Neveu

    Article

    Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

    Insert

    cassette containing the reverse tetracycline transactivator with PGK promoter
    

    Use

    Synthetic Biology

    Available Since

    May 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pECNUS_MP01

    Plasmid

    #194350

    Purpose

    CRISPR-Cas9 multiplexing acceptor clone with BpiI cutter

    Depositor

    Eunyoung Chae

    Article

    Lee et al Nucleic Acids Res. 2023 Mar 15:gkad142.

    Insert

    pcoCas9, FAST-Red
    

    Expression

    Plant

    Promoter

    RPS5A

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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