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We narrowed to 63,680 results for: cloning

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Showing: 5381 - 5400 of 63680 results

  1. pME-QF2

    Plasmid
    #83307
    Purpose
    codes for QF2 (truncated QF) in a gateway middle entry backbone.
    Depositor
    Marnie Halpern
    Insert
    QF
    Available since
    Feb. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    83307-plasmid-map-sequence-id-173284

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  5. BAP1 C91A

    Plasmid
    #81025
    Purpose
    Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker
    Depositor
    Anjana Rao
    Publication
    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.
    Insert
    BAP1 (BAP1 Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    BAP1 C91A (point mutation, catalytically inactive)
    Promoter
    5’LTR
    Available since
    Nov. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by

  6. pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9

    Plasmid
    #84246
    Purpose
    Expresses direct fusion VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    VPR-tagBFP-Sa dCas9
    Puro
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Nucleoplasmin NLS, SV40 NLS, VPR, and tagBFP
    Expression
    Mammalian
    Promoter
    CAG and PGK
    Available since
    Nov. 10, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    84246-plasmid-map-sequence-id-160889

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  7. pSLQ2833 pPB: CAG-PYL1-VPR-IRES-Zeo-WPRE-SV40PA PGK-ABI-tagBFP-SadCas9

    Plasmid
    #84243
    Purpose
    Expresses ABA-inducible VPR-Sa dCas9
    Depositor
    Stanley Qi
    Publication
    Gao et al Nat Methods. 2016 Oct 24. doi: 10.1038/n
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    ABI-tagBFP-Sa dCas9
    PYL1-VPR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    ABI-tagBFP-Nucleoplasmin NLS, IRES-Zeocin, PYL1, …
    Expression
    Mammalian
    Mutation
    D10A, N580A and Synonymous mutation in PYL1 to re…
    Promoter
    CAG and PGK
    Available since
    Nov. 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    84243-plasmid-map-sequence-id-160884

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  8. pET32a-DEST

    Plasmid
    #84652
    Purpose
    E. coli expression vector derived from pET32 with Gateway cassette.
    Depositor
    Neil Olszewski
    Type
    Empty backbone
    Tags
    His, S tag, and Trx Tag
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Nov. 7, 2016
    Availability
    Academic Institutions and Nonprofits only
    84652-plasmid-map-sequence-id-162124

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  9. pMT-sox10-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80063
    Purpose
    Sox10 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Promoter
    Sox10 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80063-plasmid-map-sequence-id-417621

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  10. pTol2p2A--3mnx1:mTagBFP-NS-MCS

    Plasmid
    #78250
    Purpose
    Can be used to subclone in gene of interest into blue motor neuron expression vector.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    -3mnx1 (mnx1 Zebrafish)
    -mTagBFP-NS-MCS
    Use
    Zebrafish motor neuron expression
    Promoter
    -3mnx1
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    78250-plasmid-map-sequence-id-149784

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  11. pME-mTagBFP

    Plasmid
    #75148
    Purpose
    TagBFP (blue) fluorophore for use with 5’ promoters.
    Depositor
    Nicholas Cole
    Publication
    Don et al Zebrafish. 2016 Sep 15.
    Insert
    mTagBFP
    Use
    Middle entry vector for multisite gateway three f…
    Promoter
    n/a
    Available since
    Oct. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    75148-plasmid-map-sequence-id-142102

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  15. pYPQ151

    Plasmid
    #69302
    Purpose
    Gateway entry vector with pcoCas9D10A
    Depositor
    Yiping Qi
    Publication
    Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    pcoCas9D10A (Plant codon-optimized)
    Use
    CRISPR
    Tags
    2XFLAG
    Mutation
    D10A mutation; NLS fusion
    Available since
    Oct. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    69302-plasmid-map-sequence-id-122140

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  16. pDestattB/CY (pCM327)

    Plasmid
    #68341
    Purpose
    Multisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafish, contains an attB site and alpha-cystallin:YFP (CY) transgenesis marker in backbone.
    Depositor
    Christian Mosimann, Leonard Zon
    Publication
    Mosimann et al Dev Dyn. 2013 Aug;242(8):949-63. doi: 10
    Type
    Empty backbone
    Use
    Zebrafish transgenesis
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    68341-plasmid-map-sequence-id-122204

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Showing: 5381 - 5400 of 63680 results