We narrowed to 29,612 results for: REP
-
Plasmid#137723PurposeDoxycycline Inducible Expression of mCherry-2A-FLAG-BRD4 long short missing its extra-terminal domain (ET)DepositorInsertBRD4 short isoform with deleted extra-terminal domain (BRD4 Human)
UseLentiviralTagsFLAGExpressionMammalianMutationDeleted amino acids 600-682PromoterTight TRE promoterAvailable sinceJan. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TE)
Plasmid#61522PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TE mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TE mutation (see comments), Tom20, and m…ExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P5-6A
Plasmid#51249PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 980,1006 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 980,1006 mutated to AlaPromoterEF1aAvailable sinceFeb. 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
Rufy3 scFv [N483/26]
Plasmid#190558PurposeMammalian Expression of Rufy3 scFV. Derived from hybridoma N483/26.DepositorInsertRufy3 (Mus musculus) recombinant scFV (Rufy3 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHtt1-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87912PurposeKamicas9 lentiviral vectorDepositorInsertshHTT1, sgCas9, cherry
UseLentiviralTagsExpressionMutationPromoterH1, 7SK, PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
TrpV1 scFv [N221/17]
Plasmid#190540PurposeMammalian Expression of TrpV1 scFV. Derived from hybridoma N221/17.DepositorInsertTrpV1 (Rattus norvegicus) recombinant scFV (Trpv1 Mouse)
UseTagsHA, Sortase, 6xHisExpressionMammalianMutationPromoterCMVAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-F-Gcn5-NLSm
Plasmid#63708Purposeretroviral expression of cytoplasm-localized mouse Gcn5 in mammalian cellsDepositorInsertGcn5 (Kat2a Mouse)
UseRetroviralTagsFLAGExpressionMammalianMutationmutated K425, K427 and K438 to R (arginine)PromoterAvailable sinceApril 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCC_07 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dxCas9-NLS-VPR-2A-Puro-WPRE
Plasmid#139092PurposeExpresses human codon-optimized inactive xCas9 3.7 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertdxCas9 3.7-VPR
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840A, A262T, R324L,S409I, E480K, E543D, M6…PromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA 3.1 SIRT1-deltaExon2
Plasmid#105671PurposeMammalian expression construct of mouse SIRT1 short isoform (lacking Exon2)DepositorInsertSIRT1 (Sirt1 Mouse)
UseTagsExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
SIN-PGK-Cas9-WPRE
Plasmid#87886PurposeTo produce Lentiviral vector for gene editingDepositorInserthCas9
UseLentiviralTagsnlsExpressionMutationHuman codon-optimizedPromotermouse PGKAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgATXN1L-1
Plasmid#74970PurposesgATXN1L-1, puromycin selectionDepositorInsertATXN1L (ATXN1L Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli3/Flag P1-4A
Plasmid#51247PurposeEncodes N-3xHA-TEV-mouseGli3-Flag-C; amino acids 849,865,877,907 mutated to AlaDepositorInsertGli3 (Gli3 Mouse)
UseFlpin systemTags3xHA-TEV and FlagExpressionMammalianMutationamino acids 849,865,877,907 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
DNMT3ACD-CRY2-EGFP
Plasmid#82556PurposeEncodes DMNT3 under LITE2.0 systemDepositorUseAAVTags2A, NLS(alpha-imp), and NLS-VP64ExpressionMammalianMutationCRY2PHR (N-terminal photolyase homology region do…PromoterEF-1alphaAvailable sinceSept. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CCR5 HHE Short
Plasmid#137697PurposeEncodes region -2761 to -1814 of CCR5 promoter HHE Haplotype fused to firefly luciferaseDepositorInsertCCR5 Promoter (CCR5 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Truncated_Codon.Optimized
Plasmid#172346PurposeExpression of FLAG-tagged, codon-optimized, truncated SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
UseTagsFLAGExpressionMammalianMutationcodon-optimized, truncated at amino acid 114 with…PromoterEF-1aAvailable sinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3 CCR5 HHG Short
Plasmid#137699PurposeEncodes region -2761 to -1814 of CCR5 promoter HHG Haplotype fused to firefly luciferaseDepositorInsertCCR5 Promoter (CCR5 Human)
UseLuciferaseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXPR_BRD003 sgATXN1L-2
Plasmid#74971PurposesgATXN1L-2, puromycin selectionDepositorInsertATXN1L (ATXN1L Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
Tom20-mChF-AIP(TD)
Plasmid#61521PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry, Tom20, and AIP with TD mutationDepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments), Tom20, and m…ExpressionMammalianMutationTD mutation (see comments)PromoterCMVAvailable sinceApril 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
Lenti CGIP + M-AAT target
Plasmid#86008Purposelenti reporter plasmid with M-AAT-specific gRNA target-sequence, to be used with tGFP #26864DepositorInsertsCAG promoter
M-AAT target
UseLentiviralTagsExpressionMutationV30MPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
mChF-AIP (TD)
Plasmid#61529PurposeExpression of human FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP (with TD mutation)DepositorInsertFK506 binding protein 1A (FKBP1A Human)
UseTagsAIP with TD mutation (see comments) and mCherryExpressionMammalianMutationPromoterCMVAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only