We narrowed to 12,952 results for: sequence

1. pRMC2-msfGFP

   Plasmid

   #194913

   Purpose

   Tetracycline inducible expression of msfGFP in Staphylococci

   Depositor

   Rikke Meyer

   Article

   Evans et al Microb Cell. 2023 Jun 28;10(7):145-156.

   Insert

   Shine Dalgarno Sequence followed by monomeric superfolder GFP
   

   Use

   Tetracycline-inducible expression vector for stap…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Use

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Promoter

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLBPVXBa-M

   Plasmid

   #229079

   Purpose

   New version of a PVX vector to express heterologous sequences in plants

   Depositor

   Jose-Antonio Daros

   Article

   Uranga et al Hortic Res. 2023 Dec 19;11(1):uhad279. d

   Insert

   Potato virus X
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Mutation coat protein start codon (ATG to AGG); d…

   Promoter

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAL113

   Plasmid

   #22471

   Purpose

   Expresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.

   Depositor

   Christopher Voigt

   Article

   Levskaya et al Nature. 2009 Oct 15;461(7266):997-1001.

   Insert

   PhyB NT (PHYB Mustard Weed, Mouse)
   

   Use

   Synthetic Biology

   Tags

   10aaLinker-mCFP-HrasCT

   Expression

   Mammalian

   Mutation

   Y276H

   Promoter

   CMV

   Available Since

   Nov. 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYL322d1/N-Vn

   Plasmid

   #183153

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at N terminus of Vn.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLVX-shRNA2-zsGreen-PGK-puro_scramble shRNA

   Plasmid

   #197991

   Purpose

   For the stable knockdown experiments using shRNA, scramble sequence was inserted as the control.

   Depositor

   Bo Wang

   Article

   Lu et al Commun Biol. 2023 Feb 17;6(1):189. doi:

   Insert

   scramble shRNA (control)
   

   Use

   Lentiviral

   Tags

   zsGreen1

   Expression

   Mammalian

   Mutation

   15q11.2 deletion

   Promoter

   Available Since

   Nov. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA3.1 (-) + SLNCR1 + 12x MS2 bs

   Plasmid

   #86828

   Purpose

   Expression of SLNCR1 lncRNA tagged with 12 copies of the MS2 stem-loop sequence

   Depositor

   Carl Novina

   Article

   Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

   Insert

   LINC00673 (LINC00673 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   June 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYL322d1/Vn-C

   Plasmid

   #183154

   Purpose

   Bimolecular fluorescence complementation (BiFC) vector for transient expression of N-truncated Venus in plants. Unique nucleotide sequences Uc and Ud for cloning at C terminus of Vn.

   Depositor

   Yao-Guang Liu, Qinlong Zhu

   Article

   Han et al Plant Biotechnol J. 2022 Feb 18. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. GST-BHMT-I/G

   Plasmid

   #104442

   Purpose

   Autophagy cargo assay

   Depositor

   Carol Mercer

   Article

   Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

   Insert

   BHMT (BHMT Human)
   

   Use

   Tags

   GFP-myc GFP is from Aequorea victoria and GST

   Expression

   Mammalian

   Mutation

   N43A in GST (in a potential KFERQ-like sequence).…

   Promoter

   CMV

   Available Since

   Dec. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. Tom70 (1-70)-GFP

    Plasmid

    #127633

    Purpose

    Mitochondrial targeting sequence of Tom70 fused to GFP

    Depositor

    Josef Kittler

    Article

    Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

    Insert

    Tom70 (TOMM70 Human)
    

    Use

    Tags

    eGFP

    Expression

    Mammalian

    Mutation

    Only first 70 amino acids

    Promoter

    Available Since

    Oct. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. RA-NES

    Plasmid

    #61019

    Purpose

    ddRFP A-NES

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddRFP A
    

    Use

    Tags

    HindIII site and NES sequence MNLVDLQKKLEELELDEQQ

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBUN421

    Plasmid

    #62204

    Purpose

    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance

    Depositor

    Qi-Jun Chen

    Article

    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    maize codon optimized

    Promoter

    TaU3 and Ubi

    Available Since

    Feb. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. LifeAct-GFP-cytoB5RR

    Plasmid

    #182577

    Purpose

    Expresses LifeAct-GFP targeted to the ER via the CytB5RR tail anchor sequence

    Depositor

    Uri Manor

    Article

    Schiavon et al Nat Methods. 2020 Sep;17(9):917-921. doi

    Insert

    LifeAct-GFP-cytoB5RR
    

    Use

    Tags

    LifeAct and cytoB5RR

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Mucolipin1-pEGFP C3

    Plasmid

    #62960

    Purpose

    full length human Mucolipin-1 cloned into pEGFP C3

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available Since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pSLQ1371-sgSTAT3-1

    Plasmid

    #121425

    Purpose

    sgSTAT3-1 sequence: GTCAGGATAGAGATAGACCAG. Lentiviral mouse U6 (mU6) promoter-driven expression vector that coexpessed Puro-T2A-mCherry from a CMV promoter.

    Depositor

    Stanley Qi, Ovijit Chaudhuri

    Article

    Lee et al Nat Commun. 2019 Apr 23;10(1):1848. doi:

    Insert

    sgSTAT3-1
    

    Use

    CRISPR and Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available Since

    April 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLZ29 (pCFJ151_Peft-3_degron_EmGFP_unc-54 3'UTR)

    Plasmid

    #71719

    Purpose

    Expressing a GFP-tagged degron in the soma of C. elegans

    Depositor

    Abby Dernburg

    Article

    Zhang et al Development. 2015 Nov 9. pii: dev.129635

    Insert

    auxin-responsive protein IAA17 (AXR3 Mustard Weed)
    

    Use

    Tags

    EmGFP

    Expression

    Worm

    Mutation

    minimal degron sequence (71-114aa) with start cod…

    Promoter

    eef-1A.1 (eft-3)

    Available Since

    Dec. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pSEZ-1

    Plasmid

    #51868

    Purpose

    Expression of cpYFP with a mitochondrial-targeting sequence (mt-cpYFP) in C. elegans

    Depositor

    Meng-Qiu Dong

    Article

    Shen et al Nature. 2014 Feb 12. doi: 10.1038/nature

    Insert

    psdhb-1::mtLS-cpYFP
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Promoter

    sdhb-1

    Available Since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGL3-BoxB

    Plasmid

    #92004

    Purpose

    Expresses firefly luciferase with BoxB sequences in 3' UTR

    Depositor

    Joshua Mendell

    Article

    Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

    Insert

    Firefly Luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 25, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-Syn-VARNAM2-Kv2.1PR

    Plasmid

    #195524

    Purpose

    Red fluorescent, negative response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    VARNAM2-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3-BaLgp120-8his

    Plasmid

    #100925

    Purpose

    Mammalian expression plasmid for gp120 subunit from the BaL HIV-1 strain

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 (BaL) Env
    

    Use

    Tags

    CD5 leader sequence and Purification tag after K5…

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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21. Luc-noPS

    Plasmid

    #177940

    Purpose

    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    Luc2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pmiCas9

    Plasmid

    #162758

    Purpose

    express miCas9

    Depositor

    Eugene Chen

    Article

    Ma et al Nat Commun. 2020 Nov 27;11(1):6082. doi:

    Insert

    miCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mammalian

    Mutation

    Contains Brex27 sequence range is 4958-5065

    Promoter

    CMV

    Available Since

    April 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pZR158_Lenti-U6-gRNA-10xCS1-PP7_hPGK-PCP-P65-HSF1-Puro

    Plasmid

    #180273

    Purpose

    Lentiviral expression vector for CRISPRa-SAM sgRNA with 10x capture sequence, and PCP-P65-HSF1 cassette

    Depositor

    Alexander Marson

    Article

    Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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24. pcDNA3-Lyn-PlcR(K30/32L)

    Plasmid

    #181831

    Purpose

    Phospholipid binding-deficient Lyn-PlcR negative-control plasmid.

    Depositor

    Jin Zhang

    Article

    Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

    Insert

    Lyn-PlcR (K30L,K32L)
    

    Use

    Tags

    ECFP, EYFP, and N-terminal targeting sequence fro…

    Expression

    Mammalian

    Mutation

    Lysines 30 and 32 in PH domain mutated to leucine.

    Promoter

    CMV

    Available Since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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25. FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA

    Plasmid

    #137827

    Purpose

    MESA split-TEV protease chain with FRB Rapamycin ectodomain, CD28 transmembrane domain, C-terminal WT TEV protease, M cleavage sequence, and tTA transcription factor

    Depositor

    Joshua Leonard

    Article

    Dolberg et al Nat Chem Biol. 2021 May;17(5):531-539. d

    Insert

    FRB-CD28TMD-CTEVp(WT)-CS(M)-tTA
    

    Use

    Tags

    3x FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pPV-EF1a-mRxxFP1(AfeI)-iP-A

    Plasmid

    #204625

    Purpose

    Cloning vector for SSA vector (mRxxFP), the target sequence can be inserted by In-Fusion reaction after AfeI digestion

    Depositor

    Akitsu Hotta

    Article

    Kita et al Stem Cell Reports. 2023 Sep 12;18(9):175

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. GFP-noPS

    Plasmid

    #177943

    Purpose

    Transient mammalian expression of eGFP. Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    eGFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJBL703

    Plasmid

    #140373

    Purpose

    Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 2BP dowsntream from the promoter

    Depositor

    Julius Lucks

    Article

    Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

    Insert

    T7-2BP-tetO-3WJdB-T
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Non-specific sgRNA

    Plasmid

    #109432

    Purpose

    MLM3636 backbone containing a gRNA that does not bind to any sequence in the human genome.

    Depositor

    Reuben Harris

    Article

    St Martin et al Nucleic Acids Res. 2018 May 9. pii: 4994

    Insert

    Non-specific gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    July 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCEP4-BG505.SOSIP-8his

    Plasmid

    #111847

    Purpose

    Mammalian expression plasmid for soluble BG505 SOSIP.664

    Depositor

    Erik Procko

    Article

    Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

    Insert

    HIV-1 Env (BG505 SOSIP.664)
    

    Use

    Tags

    8his purification tag and CD5 leader sequence

    Expression

    Mammalian

    Mutation

    Codon-optimized synthetic gene; has SOSIP mutatio…

    Promoter

    CMV

    Available Since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge