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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 12,952 results for: sequence

Showing: 8161 - 8190 of 12952 results

  1. PM-GA

    Plasmid
    #209877
    Purpose
    Dimerization dependent fluorescent protein GA anchored to cytosolic face of the plasma membrane with the N-terminal targeting sequence of human GAP43
    Depositor
    Sarah Cohen
    Article
    Miner et al Contact (Thousand Oaks). 2024 Feb 4;7:25
    Insert
    ddGFP A
    Use
    Tags
    Targeting domain of Gap43 MLCCMRRTKQVEKNDDQKI
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available Since
    Nov. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    209877-plasmid-map-sequence-id-415456

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  2. pQE-BR1

    Plasmid
    #160333
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is improved compared to wild type.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2019 Oct 30;67(43):12
    Insert
    BR1
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160333-plasmid-map-sequence-id-326604

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  3. pQE81L-KOFP7

    Plasmid
    #160330
    Purpose
    This plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its quantum yield is comparable to EGFP.
    Depositor
    Sang Taek Jung
    Article
    Ko et al J Agric Food Chem. 2020 May 27;68(21):58
    Insert
    KOFP7
    Use
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Promoter
    T5
    Available Since
    March 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    160330-plasmid-map-sequence-id-326602

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  6. cyt-GafD-mTurboID-V5

    Plasmid
    #184640
    Purpose
    Expresses V5-tagged GlycoID construct: GafD_short-miniTurboID in mammalian cytosol for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    cyt-GlycoID-V5
    Use
    Tags
    Nuclear export sequence and V5 tag
    Expression
    Mammalian
    Mutation
    GafD -> expressed aa22-178 ; BirA* -> expre…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184640-plasmid-map-sequence-id-366751

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  7. pNLS-2xBFP(4C)

    Plasmid
    #176151
    Purpose
    Encodes NLS-2xBFP(4C), which is a tandem BFP with an N-terminal SV40 NLS. The protein has 4 solvent accessible cysteines for labeling with maleimide dyes.
    Depositor
    Siegfried Musser
    Article
    Chowdhury et al Nat Cell Biol. 2022 Jan;24(1):112-122. d
    Insert
    NLS-2xBFP(4C)
    Use
    Tags
    6xHis tag and SV40 nuclear localization sequence …
    Expression
    Bacterial
    Mutation
    Compared with EGFP, the two BFP domains have the …
    Promoter
    Ptrc
    Available Since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    176151-plasmid-map-sequence-id-356068

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  13. nuc-GafD-mTurboID-HA

    Plasmid
    #184641
    Purpose
    Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging
    Depositor
    Charlie Fehl
    Article
    Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021
    Insert
    nuc-GlycoID-HA
    Use
    Tags
    3xHA tag and Nuclear localization sequence
    Expression
    Mammalian
    Mutation
    GafD -> expressed 22-178 ; BirA* -> express…
    Promoter
    CMV
    Available Since
    July 13, 2022
    Availability
    Academic Institutions and Nonprofits only
    184641-plasmid-map-sequence-id-366747

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  17. BB3cN_pGAP_23*_pPFK300_Cas9

    Plasmid
    #104911
    Purpose
    hCas9 under control of pPFK300 for direct cloning of HH-sgRNA-HDV PCR products for episomal expression in P. pastoris and selection on NTC
    Depositor
    Brigitte Gasser
    Article
    Gassler et al Methods Mol Biol. 2019;1923:211-225. doi
    Inserts
    HH - FS23 linker - HDV
    human codon-optimized hcas9 sequence fused to a nuclear localization signal (NLS)
    Use
    CRISPR
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available Since
    Jan. 14, 2020
    Availability
    Academic Institutions and Nonprofits only
    104911-plasmid-map-sequence-id-269032

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  19. pAH-CTX1-rhadCas9

    Plasmid
    #129391
    Purpose
    Derived from pAH-CTX1-rha. The Burkholderia cenocepacia codon-optimized dCas9 cloned downstream of PrhaBAD. Hence, dCas9 is controlled by the rhamnose-inducible promoter system.
    Depositor
    Silvia Cardona
    Article
    Hogan et al ACS Synth Biol. 2019 Sep 6. doi: 10.1021
    Insert
    Burkholderia cenocepacia codon-optimized dCas9
    Use
    CRISPR
    Tags
    Expression
    Bacterial
    Mutation
    Entire dCas9 codon optimized for the GC-rich B. c…
    Promoter
    PrhaBAD
    Available Since
    March 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    129391-plasmid-map-sequence-id-273754

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  20. anti-FX(a) Emi HC

    Plasmid
    #113665
    Purpose
    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FIX(a) Emi HC and the Emi LC.
    Depositor
    Robert Peters
    Article
    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1
    Insert
    Emicizumab anti-FX/FXa heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    113665-plasmid-map-sequence-id-225229

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  26. pcS-E626-C1C2

    Plasmid
    #200162
    Purpose
    This plasmid encodes EGFR targeting monobody (E626) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.
    Depositor
    Masako Harada
    Article
    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)
    Inserts
    E626
    C1C2 domain of lactadherin
    Use
    Tags
    HA and HIS
    Expression
    Mammalian
    Mutation
    sequence adopted from doi: 10.1371/journal.pone.0…
    Promoter
    CMV
    Available Since
    June 9, 2023
    Availability
    Academic Institutions and Nonprofits only
    200162-plasmid-map-sequence-id-396334

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  27. HSC1-HS4-GiP

    Plasmid
    #58540
    Purpose
    Derived from HSC1-GiP where 2 copies of the HS4 insulator core are cloned inside the 3'LTR
    Depositor
    James Ellis
    Article
    Rival-Gervier et al Mol Ther. 2013 Aug;21(8):1536-50. doi: 1
    Inserts
    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    Core HS4 insulator sequence (dimer)
    Use
    Retroviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Human EF1-alpha
    Available Since
    Sept. 3, 2014
    Availability
    Academic Institutions and Nonprofits only
    58540-plasmid-map-sequence-id-90629

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  29. anti-FIX(a) Emi HC

    Plasmid
    #113664
    Purpose
    For transient expression of Emicizumab sequence (CAS# 1610943-06-0, KEGG# D10821), IgG4 bispecific antibody targeting FIX(a) & FX(a). Must be co-expressed with the anti-FX(a) Emi HC and the Emi LC.
    Depositor
    Robert Peters
    Article
    Leksa et al J Thromb Haemost. 2019 Mar 18. doi: 10.1
    Insert
    Emicizumab anti-FIX/FIXa heavy chain
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV Promoter
    Available Since
    March 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    113664-plasmid-map-sequence-id-225231

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  30. pTS2303-Tier1-CoTC-PPGK-NLuc_TB

    Plasmid
    #169548
    Purpose
    Tier-1 expression vector encoding PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 that is insulated via a 5' CoTC insulator sequence (CoTC-PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA).
    Depositor
    Martin Fussenegger
    Article
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    CoTC-modulated PPGK-driven NanoLuc Luciferase - mTagBFP expression cassette
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    PPGK
    Available Since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169548-plasmid-map-sequence-id-364524

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Showing: 8161 - 8190 of 12952 results