We narrowed to 28,440 results for: REP

1. pCRISPomyces-AsCas12j-2

   Plasmid

   #191655

   Purpose

   All-in-one editing CRISPR-Cas construct for one-step genome editing of Streptomyces using AsCas12j-2

   Depositor

   Fong Tian Wong

   Article

   Tan et al bioRxiv 2021.10.28.465406

   Insert

   AsCas12j-2
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   rpsL(XC)-BbsI

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. XRE-H2B-mScarlet-I

   Plasmid

   #182296

   Purpose

   Fluorescent reporter for AhR activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al Environ Int. 2022 Sep 24;169:107545. doi

   Insert

   mScarlet-I
   

   Use

   Tags

   m-Scarlet-I

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-KO

   Plasmid

   #20331

   Depositor

   Rudolf Jaenisch

   Article

   Carey et al Proc Natl Acad Sci U S A. 2009 Jan 6. 10

   Insert

   Klf4-F2A-Oct4
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZipGFP-Casp3

   Plasmid

   #81241

   Purpose

   A ZipGFP-based Caspase-3 protease reporter T2A mCherry

   Depositor

   Xiaokun Shu

   Article

   To et al Cell Chem Biol. 2016 Jul 21;23(7):875-82

   Insert

   ZipGFP with caspase cleavage sequence T2A mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYIC

   Plasmid

   #18673

   Depositor

   Han Htun

   Article

   Nie et al Nucleic Acids Res. 2006 . 34(19):5528-4

   Type

   Empty backbone

   Use

   Tags

   3Myc-EYFP-HA-His6 and ECFP-HA-His6

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pNOC-ARS-stacked-NeoR-mNeonGreen

   Plasmid

   #101249

   Purpose

   Construct for fusion expression of mNeonGreen reporter and G418 resistance gene by a bidirectional promoter

   Depositor

   Eva Farre

   Article

   Poliner et al Plant J. 2019 Jul;99(1):112-127. doi: 10

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pS448∙CsR

   Plasmid

   #122096

   Purpose

   CRISPR/Cas9 counterselection in Gram-negative bacteria

   Depositor

   Pablo Ivan Nikel

   Article

   Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

   Inserts

   Cas9
   sgRNA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pem7 and Pm

   Available since

   April 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry-C1 mCherry-NLS

   Plasmid

   #58476

   Purpose

   Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

   Depositor

   Dyche Mullins

   Article

   Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

   Insert

   3X NLS with 3X stop codons
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEM4Z-EGFP

   Plasmid

   #183475

   Purpose

   This plasmid can be used to prepare EGFP mRNA by In Vitro Transcription

   Depositor

   Chantal Pichon

   Article

   Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. ART

    Plasmid

    #214063

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. ARTi

    Plasmid

    #214064

    Purpose

    Fluorescent reporter system to measure EJC-enhanced NMD activity. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3-PKA-SPARK

    Plasmid

    #106920

    Purpose

    Express PKA-SPARK, an activity reporter of protein kinase A in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    PKA-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3-ERK-SPARK

    Plasmid

    #106921

    Purpose

    Express ERK-SPARK, an activity reporter of ERK in mammalian cells.

    Depositor

    Xiaokun Shu

    Article

    Zhang et al Mol Cell. 2018 Jan 3. pii: S1097-2765(17

    Insert

    ERK-SPARK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKIR1.0

    Plasmid

    #86409

    Purpose

    CRISPR/Cas9 in Arabidopsis with seed a fluorescent reporter.

    Depositor

    Tetsuya Higashiyama

    Article

    Tsutsui et al Plant Cell Physiol. 2016 Nov 17. pii: pc

    Insert

    human-codon-optimized SpCas9
    

    Use

    CRISPR

    Tags

    FLAG and NLS

    Expression

    Mutation

    Promoter

    AtRPS5A

    Available since

    Feb. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCTCON2-GFP

    Plasmid

    #158130

    Purpose

    Single fluorescent protein reporter (GFP) for ncAA incorporation (wild-type construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    sfGFP
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GAL1-10

    Available since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. ART_RTctrl

    Plasmid

    #214066

    Purpose

    Fluorescent reporter system, readthrough control, NMD negative control. No intron in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRPA70-IRES-RPA32-IRES-RPA14

    Plasmid

    #211432

    Purpose

    mammalian expression of human RPA70 and RPA32 and RPA14

    Depositor

    Helmut Pospiech

    Insert

    RPA70 - IRES - RPA32 - IRES - RPA14 (RPA1, RPA2, RPA3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGEM4Z-Fluc

    Plasmid

    #183474

    Purpose

    This plasmid can be used to prepare Fluc mRNA by In Vitro Transcription

    Depositor

    Chantal Pichon

    Article

    Perche et al Mol Ther Nucleic Acids. 2019 Sep 6;17:76

    Insert

    Firefly luciferase
    

    Use

    Luciferase

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCRISPEY-Z3-Nat

    Plasmid

    #232100

    Purpose

    Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

    Depositor

    Jeffrey Lewis

    Article

    Stuecker et al bioRxiv [Preprint]. 2024 Aug 7:2024.08.0

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Feb. 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL2_163

    Plasmid

    #199095

    Purpose

    Level 2 plasmid, barcoded ORI with gentamicin resistance cassette and mScarlet-I reporter

    Depositor

    Charlie Gilbert, Cultivarium Tools, Nili Ostrov

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pAMβ1 ORI plus NGS barcode 3, gentamicin resistance cassette, mScarlet-I cassette, oriT
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pU6-Universal

    Plasmid

    #52694

    Purpose

    This plasmid can be used to mutate genes in Toxoplasma gondii using the CRISPR/Cas9 system after having an appropriate protospacer cloned into it.

    Depositor

    Sebastian Lourido

    Inserts

    Cas9
    Toxoplasma U6 upstream region - protospacer cloning sequence - chiRNA
    

    Use

    CRISPR; Toxoplasma gondii

    Tags

    3X FLAG and Nuclear localization signal

    Expression

    Mutation

    Promoter

    TgTUB1 and Toxoplasma U6

    Available since

    June 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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22. FDP-P10B2-A75-RZ-URA3

    Plasmid

    #130874

    Purpose

    Integration cassette for OrthoRep containing mKate driven by promoter 10B2 with a hardcoded polyA tail

    Depositor

    Chang Liu

    Article

    Zhong et al ACS Synth Biol. 2018 Dec 21;7(12):2930-2

    Insert

    mKate
    

    Use

    Expression of mkate, leu2(q180*), and ura3 on ort…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. WT-Pan

    Plasmid

    #99638

    Purpose

    EGFP-mCherry control reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. GAG-Pan

    Plasmid

    #99640

    Purpose

    EGFP-mCherry Met71GAG reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAG (Glu)

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GAA-Pan

    Plasmid

    #99639

    Purpose

    EGFP-mCherry Met71GAA reporter for methionine misincorporation

    Depositor

    Tao Pan

    Article

    Gomes et al RNA. 2016 Mar;22(3):467-76. doi: 10.1261

    Insert

    mCherry
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Met71 mutated to GAA (Glu)

    Promoter

    CMV

    Available since

    Aug. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PB-UBC-mARG3

    Plasmid

    #173793

    Purpose

    Mammalian acoustic reporter gene 1 cassette 3 on PB-UBC plasmid

    Depositor

    Xiaojun Lian

    Article

    Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

    Insert

    GvpJ, F, G, L, K
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UBC promoter

    Available since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. AiRTi

    Plasmid

    #214068

    Purpose

    Fluorescent reporter system to measure EJC-enhanced NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. AiRT

    Plasmid

    #214067

    Purpose

    Fluorescent reporter system to measure EJC-independent NMD activity. Intron present in 5'UTR

    Depositor

    Sujatha Jagannathan

    Article

    Kolakada et al Nucleic Acids Res. 2024 Feb 20:gkae121.

    Insert

    TurboRFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Linker sequence present between RFP and GFP

    Promoter

    Available since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pSCW02

    Plasmid

    #72301

    Purpose

    Starting substrate for in vitro DNA mismatch repair substrate monitored by FauI cleavage. Derived from pUC19CPDrev H. HWang and J.B. Hays 2002 JBC 277:26136.

    Depositor

    Peggy Hsieh

    Article

    Geng et al Anal Biochem. 2011 Jun 15;413(2):179-84.

    Type

    Empty backbone

    Use

    Derivative of puc19

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    lac

    Available since

    Aug. 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pLP_mod_mNeonGreen11_P2A_puroR_CRE-GFP

    Plasmid

    #229227

    Purpose

    Modular BxB1 landing pad vector containing C-term mNeonGreen11 tag, puroR marker, and a cAMP GFP reporter.

    Depositor

    Willow Coyote-Maestas

    Article

    Howard et al bioRxiv [Preprint]. 2024 Apr 18:2024.04.

    Type

    Empty backbone

    Use

    Tags

    mNeonGreen11

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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