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172,500 results

Showing: 5481 - 5500 of 172500 results

  1. pcDNA3-TLR10-YFP

    Plasmid
    #13643
    Depositor
    Doug Golenbock
    Insert
    Toll-Like Receptor 10 (TLR10 Human)
    Tags
    YFP
    Expression
    Mammalian
    Available Since
    June 12, 2007
    Availability
    Academic Institutions and Nonprofits only
    13643-plasmid-map-sequence-id-7721

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  3. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Expression
    Mammalian
    Promoter
    EF1α and pTRE3G
    Available Since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  5. pEF-GalNAcT2-msGFP2

    Plasmid
    #218965
    Purpose
    Expresses full length GalNAcT2-msGFP2 mammalian cells
    Depositor
    Benjamin Glick
    Insert
    GalNAcT2-msGFP2 (GALNT2 Human)
    Tags
    msGFP2
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    Sept. 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    218965-plasmid-map-sequence-id-446216

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  6. Inducible Caspex expression

    Plasmid
    #97421
    Purpose
    For stable clone generation of cells expressing the dCas9-APEX2 fusion protein for Genomic locus proteomics
    Depositor
    Steven Carr, Samuel Myers
    Article
    Myers et al Nat Methods. 2018 May 7. pii: 10.1038/s4
    Inserts
    Caspex
    T2A-Gfp
    Use
    CRISPR
    Tags
    3xFLAG and V5
    Expression
    Mammalian
    Mutation
    dCas9: D10A and H840A, APEX2: K14D, W41F, E112K, …
    Promoter
    TRE3G
    Available Since
    Oct. 23, 2017
    Availability
    Academic Institutions and Nonprofits only
    97421-plasmid-map-sequence-id-466895

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  7. TFORF2910

    Plasmid
    #143665
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NFIC (NFIC Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143665-plasmid-map-sequence-id-400441

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  10. pST50Trc3-HISNDHFR

    Plasmid
    #63976
    Purpose
    Position 3 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63976-plasmid-map-sequence-id-177382

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  11. pSupAR-MbPylRS(DiZPK)

    Plasmid
    #91705
    Purpose
    express Methanosarcina barkeri pyrrolysyl-tRNA synthetase/tRNA pair in E. coli to incorporate photocrosslinkers DiZPK, DiZSeK, or DiZHSeC into an in-frame amber codon site on a protein of interest
    Depositor
    P. Chen
    Article
    Zhang et al Nat Chem Biol. 2011 Sep 4;7(10):671-7. d
    Inserts
    pylS
    pylT
    Expression
    Bacterial
    Mutation
    L274A, C313S
    Available Since
    July 20, 2017
    Availability
    Academic Institutions and Nonprofits only
    91705-plasmid-map-sequence-id-183278

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  12. pST50Trc4-HISNDHFR

    Plasmid
    #63994
    Purpose
    Position 4 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    63994-plasmid-map-sequence-id-177400

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  16. pST50Trc2-HISNDHFR

    Plasmid
    #63958
    Purpose
    Position 2 transfer plasmid for pST44 polycistronic plasmid suite; N-term cleavable single tag; contains DHFR gene in BamHI-NgoMIV cassette as positive control
    Depositor
    Song Tan
    Article
    Tan et al Protein Expr Purif. 2005 Apr;40(2):385-9
    Type
    Empty backbone
    Tags
    6xHis; TEV cleavable
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    Oct. 7, 2015
    Availability
    Academic Institutions and Nonprofits only
    63958-plasmid-map-sequence-id-177364

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  17. pSLC-255

    Plasmid
    #124292
    Purpose
    Expression of vsfGFP-9. This construct improves brightness of sfGFP by fusing a GFP-specific single domain antibody to it that creates a monomeric fluorophore.
    Depositor
    Swaine Chen
    Article
    Eshaghi et al Angew Chem Int Ed Engl. 2015 Nov 16;54(4
    Insert
    vsfGFP-9
    Expression
    Bacterial
    Available Since
    Sept. 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    124292-plasmid-map-sequence-id-243084

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Showing: 5481 - 5500 of 172500 results