We narrowed to 22,939 results for: REP
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Plasmid#177559PurposeMammalian Expression Plasmid of anti-c-Fos (Human). Derived from hybridoma N486/32.DepositorInsertanti-c-Fos (Homo sapiens) recombinant mouse monoclonal antibody (FOS Mouse)
ExpressionMammalianPromoterDual CMVAvailable SinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-GSAS
Plasmid#164565PurposeSARS-CoV-2 Spike protein (S-GSAS variant)DepositorInsertSpike (S-GSAS variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationEctodomain (AAs 1-1208); 682-685 (furin site = RR…Available SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pαH-S-RRAR/D614G
Plasmid#164570PurposeSARS-CoV-2 Spike protein with D614G mutation and Furin site restored (S-RRAR-D614G variant)DepositorInsertSpike (S-RRAR-D614G variant)
Tags2X Strep-Tag II, 8X His tag, and HRV3C cleavage s…ExpressionMammalianMutationD614GAvailable SinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJOP-HTT-HR30Q
Plasmid#92247PurposeHTT donor DNA with 30Q for homologous recombination-mediated gene modificationDepositorInsertHTT donor DNA (HTT Human)
UsePrecisionx hr targeting vectorMutationBarcode of wobble bases to prevent TALEN cleaving…Available SinceFeb. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
AF20-double-tag
Plasmid#108389PurposeExpress human PC1DepositorInsertPKD1 (PKD1 Human)
TagseGFP-TEV cleavage-S15 and mcherry-TEV cleavage-V5ExpressionMammalianPromoterCMVAvailable SinceAug. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
p-mCherry-C1-iC++_CA
Plasmid#98168Purposeslow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with cyan light, inactivation max with 590 nm (accelarates closure to ms). Codon optimized for mammalian expression.DepositorInsertSynthetic construct iC++_C128A gene
TagsmCherryExpressionMammalianMutationT59S, E83N, E90Q, E101S, V117R, E123S, C128A, V24…PromoterCMV (+enhancer)Available SinceJuly 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC292 - pAAV EF1a floxed hChR2(H134R)-EYFP
Plasmid#50834PurposeAn AAV packaging vector that expresses channel rhodopsin 2 (H134R) (fused to EYFP) under the EF1a promoter, which can be deactivated (deleted) by recombination between the flanking loxP sites.DepositorInserthChR2 (H134R)
UseAAV and Cre/LoxTagsEYFPExpressionMammalianMutationH134RPromoterEF1aAvailable SinceOct. 9, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLV-H2B-iRFP670-p2a-mCerulean-Geminin (1–110)-IRES-Neomycin
Plasmid#223959PurposeDual fluorescent reporter for histone H2B and for APC/C activityDepositorUseLentiviralTagsiRFP670 and mCeruleanExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pmCherryC1 VAP-A KD/MD
Plasmid#226408PurposeExpression of human VAP-A (mutant K94D/M96D, unable to bind FFAT motifs) fused to mCherry in mammalian cellsDepositorInsertVAP-A (VAPA Human)
TagsT7-Xpress tag and mCherryExpressionMammalianMutationK94D/M96D mutant, unable to bind FFAT motifsAvailable SinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTwist-FLAG-SRSF3_Full.Length_Codon.Optimized
Plasmid#172345PurposeExpression of FLAG-tagged, codon-optimized, full-length SRSF3DepositorInsertserine and arginine rich splicing factor 3 (SRSF3 Human)
TagsFLAGExpressionMammalianMutationcodon-optimizedPromoterEF-1aAvailable SinceSept. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4/TO/GFPZNF598-9P-9A
Plasmid#141192PurposeExpresses GFP-ZNF598 mutated in polyproline streches in mammalian cells, can be used to make inducible cell lineDepositorInsertZNF598 (ZNF598 Human)
TagsGFPExpressionMammalianMutationall 3 proline repeats are mutated to Alanine (9xP…PromoterCMVAvailable SinceJune 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBBA57-TEV-His12
Plasmid#137033Purposeexpression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BBA57 with C-terminal TEV-protease-cleavable His12DepositorInsertAAC66270.1 (BB_A57 Borrelia burgdorferi B31)
TagsHis12 (TEV protease cleavable)ExpressionBacterialMutationwild type, full-length BBA57, contains signal pep…PromoterT7Available SinceMarch 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
AAV8-hSyn-flex-miR30-eGFP-shCrh
Plasmid#132715PurposeEncodes Cre-dependent short hairpin RNA (shRNA) that targets the 3’-untranslated region of the rat Crh gene which encodes corticotropin releasing factorDepositorAvailable SinceOct. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
SIN-cPPT-H1-sgHTT1-U6-sgGFP2-7SK-sgCas9-PGK-mCherry-WPRE
Plasmid#87919PurposeHIV-1 SIN lentiviral transfer vectorDepositorInsertsgHTT1, shGFP2, sgCas9, cherry
UseLentiviralPromoterH1, U6, 7SK, PGKAvailable SinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET-Hia5-OPT1
Plasmid#231968PurposeExpresses DNA adenine N6-methyltransferase Hia5 in Escherichia coli (E.coli) T7 Express lysY/IqDepositorInsertDNA adenine N6-methyltransferase Hia5
TagsHistidine tagExpressionBacterialAvailable SinceSept. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pDEST17-His10-ccdB
Plasmid#166145PurposeGateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.DepositorTypeEmpty backboneTagsHis10ExpressionBacterialAvailable SinceMay 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)
Plasmid#197885PurposeCan be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without CreDepositorInsertGFP
UseAAVMutationN/APromoterCAGAvailable SinceMarch 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCF806-shABC2
Plasmid#186714PurposeExpresses dox-controlled miR-E shRNAs (UT4GEPIR). Multi-miR shRNAs targeting A-RAF, B-RAF, and C-RAF (shABC2 = shARAF.169-shBRAF.1296-shCRAF.387). The shABC2 set is better than shABC1.DepositorAvailable SinceJan. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSems-leader-mXFPe-IFNGR2(30-337)
Plasmid#192787PurposeExpression of mXFPe-tagged IFNGR2 at the plasma membrane, e.g. for single molecule fluorescence microscopyDepositorInsertIFNGR2 (IFNGR2 Human)
TagsIg k-chain leader sequence and mXFPeExpressionMammalianMutationmXFPe: tyrosine 66 to phenylalanine, asparagine 1…PromoterCMVAvailable SinceDec. 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
Tol2 8xSTAR-mNeonGreen. PGK-puro
Plasmid#136255PurposeFluorescent reporter for intestinal stem cells through ASCL2 transcriptional activityDepositorInsertstem cell Ascl2 reporter, 8 repeats
UseTol2 transposaseTagsmNeonGreenExpressionMammalianAvailable SinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only