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Lenti-RNF2-ngRNA+41_EF1a-puroR

Plasmid

#207358

Purpose

Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

Depositor

Marianne Carlon

Article

Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

Insert

RNF2 +41 ngRNA

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

April 19, 2024

Availability

Academic Institutions and Nonprofits only

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Anti-OCRL/INPP5b [N166A/26]

Plasmid

#190301

Purpose

Mammalian Expression Plasmid of anti-OCRL/INPP5b (Human). Derived from hybridoma N166A/26.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-OCRL/INPP5b (Homo sapiens) recombinant Mouse monoclonal antibody (OCRL Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV5

Plasmid

#74984

Purpose

Constitutive ETV5 ORF (codon optimized), blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Inserts

ETV5 (ETV5 Human)
ETV5 (ETV5 Human)

Use

Tags

Expression

Mammalian

Mutation

Codon optimized (synonymous)

Promoter

Available since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xhyb PylT A41AA C55A CRFR1 95TAA 263TAG-HA

Plasmid

#154780

Purpose

plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and rat CRFR1 95TAA 263TAG, for transient transfection or stable, piggybac-mediated, integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

Insert

CRFR1 (Crhr1 Rat)

Use

Tags

HA

Expression

Mammalian

Mutation

95TAA 263TAG in CRFR1-HA, hybrid PylT with A41AA …

Promoter

EF1

Available since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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lenti-SpCas9 blast

Plasmid

#104997

Purpose

This lentiviral construct delivers hSpCas9 and blasticidin S resistance.

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

KpnI-XhoI-BsrGI-NheI

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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AIP(WT)-mChF-giantin

Plasmid

#61523

Purpose

Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Inserts

FK506 binding protein 1A (FKBP1A Human)
golgin B1 (GOLGB1 Human)

Use

Tags

AIP and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
GST-FUS-WT

Plasmid

#44978

Depositor

Robin Reed

Article

Yamazaki et al Cell Rep. 2012 Oct 25;2(4):799-806. doi:

Insert

FUS (Full length) (FUS Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

June 3, 2013

Availability

Academic Institutions and Nonprofits only
pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Use

Tags

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Promoter

Available since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM7 [N74/25R]

Plasmid

#177569

Purpose

Mammalian Expression Plasmid of anti-TrpM7 (Mouse). Derived from hybridoma N74/25.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM7 (Mus musculus) recombinant mouse monoclonal antibody (Trpm7 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pF CAG BFP2 IRES neo

Plasmid

#108175

Purpose

Expresses a blue fluorescent protein (BFP2) in mammalian cells along with conferring resistance to G418

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Insert

Blue fluorescent protein (BFP2)

Use

Lentiviral; Fluorescent protein

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 SIRT7_H187Y

Plasmid

#53151

Purpose

expression vector for catalytically inactive human Sirtuin7

Depositor

Katrin Chua

Article

Shin et al Cell Rep. 2013 Nov 14;5(3):654-65. doi:

Insert

Sirt7 (SIRT7 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

H187Y, catalytic inactive

Promoter

CMV

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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Anti-TrpM6 [N470/22R]

Plasmid

#206596

Purpose

Mammalian Expression Plasmid of anti-TrpM6 (Human). Derived from hybridoma N470/22.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-TrpM6 (Homo sapiens) recombinant Mouse monoclonal antibody (TRPM6 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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IRSp53/BAIAP2 scFv [L117/1]

Plasmid

#190506

Purpose

Mammalian Expression of IRSp53/BAIAP2 scFV. Derived from hybridoma L117/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

IRSp53/BAIAP2 (Homo sapiens) recombinant scFV (BAIAP2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKD4-ChR2-YFP

Plasmid

#178721

Purpose

AAV vector for Cre- and Flp-dependent transgene expression (CRE-ON / FLP-ON) of ChR2-YFP in cortical interneurons under the control of the Dlx enhancer (Dimidschstein et al 2016)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

ChR2-YFP

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS2-Dreadd-hM3Dq-HA-P2A-tBFP

Plasmid

#178707

Purpose

AAV vector for Cre-dependent transgene expression of Dreadd-hM3Dq-HA-P2A-tBFP in neurons under the control of the hSyn1 promoter (Kugler et al 2003)

Depositor

Jordane Dimidschstein

Article

Pouchelon et al Cell Rep Methods. 2022 May 26;2(6):10022

Insert

Dreadd-hM3Dq-HA-P2A-tBFP

Use

AAV

Tags

Expression

Mutation

Promoter

hSyn

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNL-CEF-CD79B-RFP

Plasmid

#187005

Purpose

Expression of human CD79B as RFP fusion protein

Depositor

Hermann Eibel

Article

Sevdali et al Cell Rep. 2022 Jun 28;39(13):111019. doi

Insert

CD79B (CD79B Human)

Use

Lentiviral

Tags

tagRFP

Expression

Mutation

Promoter

Available since

July 25, 2022

Availability

Academic Institutions and Nonprofits only

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pLL1 sgRNA(MS2)-MS2-AIDmono

Plasmid

#112127

Purpose

empty sgRNA cloning vector with MS2-AIDmono

Depositor

Fei-Long Meng

Article

Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

Insert

AID mono mutant (AICDA Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

N- mutated and C- terminal truncated, H130A/R131E…

Promoter

hU6,CBh

Available since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pTRIP-CMV-Puro-2A-NLS-FLAG-cGAS

Plasmid

#127656

Purpose

Over-express NLS-FLAG-cGAS

Depositor

Nicolas Manel

Article

Gentili et al Cell Rep. 2019 Feb 26;26(9):2377-2393.e1

Insert

cGAS (CGAS Human)

Use

Lentiviral

Tags

Flag

Expression

Mutation

Promoter

Available since

July 11, 2019

Availability

Academic Institutions and Nonprofits only

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pF CAG luc IRES neo

Plasmid

#98294

Purpose

For constitutive expression of firefly luciferase from the CAG promoter together with an IRES-linked neo resistance gene

Depositor

Brett Stringer

Article

Stringer et al Sci Rep. 2019 Mar 20;9(1):4902. doi: 10.

Inserts

Firefly luciferase
IRES
Neomycin resistance gene (NeoR)

Use

Lentiviral and Luciferase

Tags

Expression

Mutation

Promoter

Available since

July 5, 2017

Availability

Academic Institutions and Nonprofits only

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Anti-QKI-6 [N182/17R-2b]

Plasmid

#206661

Purpose

Mammalian Expression Plasmid of anti-QKI-6 (Human). Derived from hybridoma N182/17-2b.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-QKI-6 (Homo sapiens) recombinant Mouse monoclonal antibody (QKI Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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