We narrowed to 15,685 results for: genome
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psiCHECK2-2A-3xFLAG-SBP-2A
Plasmid#159584PurposeLuciferase reporter for ribosome pausing controlDepositorInserts2A-3xFLAG-SBP-2A
EIF2S3 5UTR
UseLuciferaseExpressionMammalianAvailable SinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
lenti-KRAS-G12C-tmpknot-epegRNA
Plasmid#214094PurposeLentiviral vector expressing epegRNA to induce KRAS G12C mutationDepositorInsertKRAS G12C epegRNA
UseLentiviralExpressionMammalianPromoterhU6Available SinceJune 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB2988
Plasmid#63638PurposepCfB2988 is a vector for multiple integrations at sites sharing homology with Ty1Cons2. The selective marker is Kl.URA3. Additionally it contains a USER cassette.DepositorTypeEmpty backboneUseCre/LoxExpressionYeastPromoternoneAvailable SinceAug. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_AAVS1B
Plasmid#183338PurposeAll-in-One CRISPRko system with a guide RNA that targets the AAVS1 locusDepositorInsertAAVS1B
UseLentiviralAvailable SinceMay 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
ePPE-V223A
Plasmid#205243PurposeFor plant prime editing in wheat plants or monocotyledons protoplastsDepositorInsertSV40_NLS-nCas9-NC-MLV-SV40_NLS
UseCRISPRExpressionPlantMutationDetailed in manuscriptAvailable SinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pU6-DHFR
Plasmid#80329PurposeAllows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamineDepositorInsertDHFR-TSc3
UseCRISPRPromoterDHFR-TS 5' UTRAvailable SinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
eGFPbait-E2A-KalTA4-pA donor vector
Plasmid#61069Purposefor CRISPR/Cas9 mediated insertion of E2A-KalTA4; to be used in combination with a eGFP specific sgRNA (e.g. Plasmid 61051)DepositorInsertseGFPbait
KalTA4
UseZebrafish expressionTagsE2AAvailable SinceFeb. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pKMD106e - intein-Cas9(S219)
Plasmid#64190PurposeExpresses intein-Cas9(S219) in mammalian cellsDepositorInsertmammalian codon-optimized S. pyogenes intein-Cas9(S219) - NLS - 3x FLAG
UseCRISPRTagsNLS - 3x FLAG (C terminal on insert)ExpressionMammalianPromoterCMVAvailable SinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
TagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialAvailable SinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-BE-VQRCas9
Plasmid#101740Purposeexpression of an optimized Cas9 for base-editing in zebrafishDepositorInsertrAPOBEC1-XTEN-zCas9(VQR)-UGI-NLS
UseCRISPRExpressionMammalianMutationD10A, VQR mutationsPromoterCMVAvailable SinceJan. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP2B
Plasmid#183325PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP2B geneDepositorInsertTOP2B
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_TOP2A
Plasmid#183324PurposeAll-in-One CRISPRko system with a guide RNA that targets TOP2A geneDepositorInsertTOP2A
UseLentiviralAvailable SinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCI Cas9 H2B-RFP
Plasmid#92397PurposeCAG-driven ubiquitous expression of Cas9 with Histone2B-RFP reporter controlled by IRESDepositorInsertCas9
UseCRISPRExpressionMammalianPromoterCAGAvailable SinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFnCas12a_NT
Plasmid#169838PurposeExpresses FnCas12a and guide RNA in BacteriaDepositorInsertFnCas12a
UseCRISPRExpressionBacterialPromoterpXynAAvailable SinceJune 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR3
Plasmid#183291PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR3 geneDepositorInsertFGFR3
UseLentiviralAvailable SinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDR-GFPuniv
Plasmid#46085PurposeIn vivo recombination assay fluorescent reporterDepositorInsertDR-GFPuniv reporter
UseRecombination reporter plasmidExpressionMammalianMutationE5G and K163R mutations in EGFP relative to wild-…Available SinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits only
