We narrowed to 18,127 results for: Mut

1. pMX1418

   Plasmid

   #127856

   Purpose

   Expresses EGFP-IPNN-MCAK in mammalian cells

   Depositor

   Linda Wordeman

   Article

   Domnitz et al J Cell Biol. 2012 Apr 16;197(2):231-7. d

   Insert

   IPNN-MCAK
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   mutation is I97N and P98N

   Promoter

   CMV

   Available Since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. Rag2 T490A

   Plasmid

   #115650

   Purpose

   expression of full length Rag2 with T490A mutation

   Depositor

   Martin Gellert

   Article

   Grundy et al Proc Natl Acad Sci U S A. 2010 Dec 28;10

   Insert

   Rag2 (Rag2 Mouse)
   

   Use

   Tags

   8XHis-MBP-PreScission

   Expression

   Mammalian

   Mutation

   T490A

   Promoter

   Available Since

   Sept. 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTU2-a-RFP

   Plasmid

   #72958

   Purpose

   Level 2 Destination vector - Position A - Assembly of 2 TU's from Level 1-AB

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 2 - a site
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

4. pAC-EsaR-V220A

   Plasmid

   #47645

   Purpose

   pAC-EsaR with a c659t mutation in esaR

   Depositor

   Cynthia Collins

   Article

   Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

   Insert

   esaR-V220A
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Changed Valine 220 to Alanine

   Promoter

   Plac

   Available Since

   Sept. 6, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pEB2-Katushka-9-5

   Plasmid

   #104011

   Purpose

   Plasmid encoding Katushka-9-5

   Depositor

   Philippe Cluzel

   Article

   Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

   Insert

   Katushka9-5
   

   Use

   Low copy

   Tags

   Expression

   Bacterial

   Mutation

   Mutations relative to eqFP578 (K6S, N21D, R32G, L…

   Promoter

   proC

   Available Since

   Dec. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pCDNA HA FAK Y861F

   Plasmid

   #50508

   Depositor

   Kenneth Yamada

   Article

   Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

   Insert

   protein tyrosine kinase, mutated
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Y861F

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pEGFP-N1-mSNX27-H112A

   Plasmid

   #163619

   Purpose

   mouse SNX27 cDNA with a point mutation H112A C-terminally tagged with GFP

   Depositor

   Josef Kittler

   Article

   Halff et al Cell Rep. 2019 Nov 26;29(9):2599-2607.e6

   Insert

   mouse SNX27-H112A (Snx27 Mouse)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   H112A

   Promoter

   Available Since

   Jan. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENTR-L3-oPRE-L2

   Plasmid

   #32415

   Depositor

   Matthew Nolan

   Article

   White et al Front Mol Neurosci. 2011;4:8. Epub 2011

   Insert

   oPRE
   

   Use

   Gateway cloning vector

   Tags

   Expression

   Mutation

   WPRE with deletion of X protein promoter and muta…

   Promoter

   Available Since

   Jan. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. cacna2d1 R241A D491A HA pcDNA3

   Plasmid

   #206109

   Purpose

   expression of rat alpha2delta-1 calcium channel auxillary subunit with a single HA tag inserted after aa549 and with R421A and D491A mutations that both disrupt Gabapentin binding

   Depositor

   Annette Dolphin

   Insert

   cacna2d1 (Cacna2d1 Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R241A D491A

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-PKCZeta KR HA Tagged

    Plasmid

    #24608

    Depositor

    Jeff Wrana

    Article

    Wang et al Science. 2003 Dec 5. 302(5651):1775-9.

    Insert

    PKCZeta (Prkcz Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281R mutation in the ATP binding domain causing …

    Promoter

    Available Since

    June 7, 2010

    Availability

    Industry, Academic Institutions, and Nonprofits

11. pSABAD505

    Plasmid

    #45615

    Depositor

    Hideo Iwai

    Article

    Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

    Insert

    GFPN-NpuDnaE_intein-GFP_C
    

    Use

    Tags

    GFP_C and GFP_N

    Expression

    Bacterial

    Mutation

    stemmer GFP with superfolder mutations S30R and Y…

    Promoter

    pBAD

    Available Since

    Sept. 13, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pICH87633

    Plasmid

    #50271

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter, nos, (Agrobacterium tumefaciens) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. nonphosphorylatable Mis12-targeted Aurora B FRET sensor

    Plasmid

    #45229

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    non phosphorylatable Mis12-targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T-A mutation in substrate sequence

    Promoter

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBP-TL5

    Plasmid

    #72989

    Purpose

    RBS_M2

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    M2 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pBP-TL11

    Plasmid

    #72995

    Purpose

    RBS_L4

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L4 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pENTR2-R2-PBR-L4

    Plasmid

    #141017

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pENTR2-L3-PBL-R1

    Plasmid

    #141016

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. Drp1(A395D)-StrepII

    Plasmid

    #174430

    Purpose

    Expresses human Drp1 Isoform 3 with A395D mutation in bacteria

    Depositor

    Thomas Pucadyil

    Article

    Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

    Insert

    Drp1
    

    Use

    Tags

    StrepII

    Expression

    Bacterial

    Mutation

    A395D

    Promoter

    T7

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. piiTRE-Bi-SG-Tii

    Plasmid

    #26121

    Depositor

    Liqun Luo

    Article

    Li et al PLoS One. 2010 . 5(7):e11503.

    Insert

    Synaptophysin (Syp Mouse)
    

    Use

    Tags

    mut4EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 1, 2010

    Availability

    Academic Institutions and Nonprofits only

20. pDONR-P2rP4

    Plasmid

    #141015

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. Myog'-2A-mCherryNLS-PGK-Puro

    Plasmid

    #69548

    Purpose

    Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

    Depositor

    Charles Gersbach

    Inserts

    2A-mCherry-NLS
    Myogenin 5' homology arm
    Myogenin 3' homology arm (Myog Mouse)
    

    Use

    Mouse Targeting

    Tags

    2x nuclear localization signal and PVT1-2A "…

    Expression

    Mutation

    Silent V205V mutation in myogenin gene to prevent…

    Promoter

    Available Since

    April 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. LV anti-FITC-LaG16-Y192A-T2A-BFP

    Plasmid

    #194933

    Purpose

    LV construct encoding Y192A mutant sNRR-based SynNotch receptor

    Depositor

    John Ngo

    Article

    Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

    Insert

    antiFITC-LaG16-sNRR(Y192A)-SynNotch-Gal4-VP64-T2A-BFP
    

    Use

    Lentiviral and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGK415-­rtetTAK8N, L131L

    Plasmid

    #165969

    Purpose

    Expresses rTetTA (K8N, L131L) mutant

    Depositor

    Jun Ishii

    Article

    Tominaga et al Nat Commun. 2021 Mar 23;12(1):1846. doi:

    Insert

    tetR
    

    Use

    Synthetic Biology

    Tags

    three copies of VP16 (VP48)

    Expression

    Yeast

    Mutation

    Promoter

    pPGK1

    Available Since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEB1-Clover

    Plasmid

    #103991

    Purpose

    Plasmid encoding Clover

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Clover
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (S30R, Y39N, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pICSL70002

    Plasmid

    #50333

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    Biotin Selection Cassette (Act2 promoter (A. thaliana) + BirA (E.coli) + 35s terminator (Cauliflower Mosaic Virus))
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pATD

    Plasmid

    #141012

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    HSV-TK
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    July 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pENTR-L5-oPRE-L2

    Plasmid

    #32414

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    oPRE
    

    Use

    Gateway cloning vector

    Tags

    Expression

    Mutation

    WPRE with deletion of X protein promoter and muta…

    Promoter

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pTU1-B-RFP

    Plasmid

    #72940

    Purpose

    Level 1 Destination vector - Position B

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - B site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDRF1-GW AKAR3-EV-NR

    Plasmid

    #182534

    Purpose

    Expresses the non responsive PKA FRET sensor AKAR3-EV (T506A) in budding yeast

    Depositor

    Bas Teusink

    Article

    Botman et al FEMS Yeast Res. 2023 Jan 4;23:foad029. d

    Insert

    AKAR3-EV-NR
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    T506A mutation

    Promoter

    PMA1

    Available Since

    Oct. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEB1-Venus SX

    Plasmid

    #103990

    Purpose

    Plasmid encoding Venus SX

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    Venus SX
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available Since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pEB1-mVenus JBC

    Plasmid

    #103987

    Purpose

    Plasmid encoding mVenus JBC

    Depositor

    Philippe Cluzel

    Article

    Balleza et al Nat Methods. 2018 Jan;15(1):47-51. doi:

    Insert

    mVenus JBC
    

    Use

    Low copy

    Tags

    Expression

    Bacterial

    Mutation

    Mutations relative to wild-type GFP (F46L, F64L, …

    Promoter

    proC

    Available Since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pCDNA HA FAK Y576/577F

    Plasmid

    #50505

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y576/577F

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

33. pReporter_9

    Plasmid

    #60569

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pJW1510

    Plasmid

    #62364

    Purpose

    pFA6a pSEC63-BirA-mVenus-UBC6-TA::HIS5

    Depositor

    Jonathan Weissman

    Article

    Jan et al Science. 2014 Nov 7;346(6210):1257521. d

    Insert

    BirA-mVenus-Ubc6 tail anchor
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    L232H mutation compared to standard mVenus

    Promoter

    Sec63

    Available Since

    May 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pBS7

    Plasmid

    #83774

    Purpose

    Useful for tagging yeast proteins with C-terminal Venus tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    Venus
    

    Use

    Tags

    Venus

    Expression

    Yeast

    Mutation

    F46L, F64L, S65G, V68L, Q69M, S72A, Q80R, M153T, …

    Promoter

    Available Since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pAc5.1B-lambdaN-HA-DmFMR1_B

    Plasmid

    #145940

    Purpose

    Insect Expression of DmFMR1

    Depositor

    Elisa Izaurralde

    Insert

    DmFMR1 (Fmr1 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Two amino acid mutations (N45D and G558R) and thr…

    Promoter

    Available Since

    Feb. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pICH51277

    Plasmid

    #50268

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    promoter (0.4 kb), 35s (Cauliflower Mosaic Virus) + 5'UTR, omega (Tobacco Mosaic Virus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-myc-Rem2 RNAiR2

    Plasmid

    #51585

    Purpose

    expresses RNAi-resistant (RNAiR) myc-tagged mouse Rem2 (long version)

    Depositor

    Suzanne Paradis

    Article

    Ghiretti et al Dev Neurobiol. 2011 May;71(5):374-89. do

    Insert

    Rem2 (Rem2 Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    silent mutations at shRNA 1 and 2 recognition sit…

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pBP-TL4

    Plasmid

    #72988

    Purpose

    RBS_H5

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    H5 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

40. pBP-TL8

    Plasmid

    #72992

    Purpose

    RBS_L1

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    L1 RBS
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    CAT gene - C435G (nucleotide) - silent mutagenesi…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only