We narrowed to 148,253 results for: ins;

1. pUPD2-HR1b-GFP-L (B3-B4)

   Plasmid

   #211853

   Purpose

   E3-DART degron casette for N-terminal fusion to protein of interest using Goldenbraid

   Depositor

   Marcela Rojas-Pierce

   Article

   Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

   Insert

   HR1b
   

   Use

   Synthetic Biology

   Tags

   GFP

   Available Since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRAU168

   Plasmid

   #86834

   Purpose

   bacterial expression of anti-CRISPR protein AcrIIA1

   Depositor

   Joseph Bondy-Denomy

   Article

   Rauch et al Cell. 2016 Dec 22. pii: S0092-8674(16)31

   Insert

   acrIIA1
   

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Aug. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDRESS_mTurquoise2_spatial-linker-P2A_mScarlet

   Plasmid

   #130509

   Purpose

   expresses mScarlet

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Protoc. 2020 Feb;15(2):450-478. doi:

   Insert

   mScarlet
   

   Expression

   Mammalian

   Available Since

   Nov. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV mouse sidekick2

   Plasmid

   #18735

   Depositor

   Joshua Sanes

   Article

   Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

   Insert

   Sidekick-2 (Sdk2 Mouse)
   

   Expression

   Mammalian

   Available Since

   Aug. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

5. pLSLGFP

   Plasmid

   #51494

   Purpose

   pLSL with GFP followed by the us:NPTII repair template, Rep is not included on this plasmid

   Depositor

   Daniel Voytas

   Article

   Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

   Inserts

   GFP
   us:NPTII repair template
   

   Use

   Plant t-dna plasmid

   Tags

   NLS and flag

   Promoter

   virion-sense LIR and 2x35S

   Available Since

   May 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28-6His-SUMO-Nsp10

   Plasmid

   #154959

   Purpose

   Expresses Nsp10 from SARS-CoV-2 with a N-terminal SUMO-protease cleavable 6His-SUMO tag in E. coli

   Depositor

   Justin Kollman

   Insert

   6His-SUMO-Nsp10 (ORF1ab SARS-CoV-2)
   

   Tags

   6His-Thrombin-SUMO

   Expression

   Bacterial

   Promoter

   T7 Promoter

   Available Since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pC.HA Vav1

   Plasmid

   #14553

   Depositor

   Joan Brugge

   Article

   Moores et al Mol Cell Biol. 2000 Sep . 20(17):6364-73

   Insert

   Vav1 (VAV1 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   April 12, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSG66-KcsA-Kv1.3-sfGFP

   Plasmid

   #102457

   Purpose

   Expresses a fusion protein of KcsA-Kv1.3 and sfGFP with a C-terminal His tag. The expression is controlled by a OR2-OR1-Pr promoter.

   Depositor

   Richard Murray

   Article

   Guo et al bioRxiv 104455

   Insert

   KcsA-Kv1.3
   

   Tags

   His tag and sfGFP

   Expression

   Bacterial

   Promoter

   OR2-OR1-Pr

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRK5 HA GST rap2a

   Plasmid

   #14952

   Depositor

   David Sabatini

   Article

   Sancak et al Mol Cell. 2007 Mar 23. 25(6):903-15.

   Insert

   rap2a (RAP2A Human)
   

   Tags

   HA-GST

   Expression

   Mammalian

   Available Since

   May 10, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pUPD2-HR1bR181A R185A-C (B3-B4)

    Plasmid

    #211855

    Purpose

    CDS for mutant HR1b degron in domestication vector

    Depositor

    Marcela Rojas-Pierce

    Article

    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.

    Insert

    HR1b
    

    Use

    Synthetic Biology

    Mutation

    R181A R185A

    Available Since

    March 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. piRFP682-N1

    Plasmid

    #45459

    Depositor

    Vladislav Verkhusha

    Article

    Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

    Insert

    iRFP682
    

    Expression

    Mammalian

    Mutation

    T2A, S13L, A92T, V104T, V114I, E161K, E180V, Y193…

    Promoter

    CMV

    Available Since

    June 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pccGFPGpA

    Plasmid

    #73651

    Purpose

    Fluorescent reporter for transmembrane protein dimerization positive control.

    Depositor

    Alessandro Senes

    Article

    Armstrong et al Biochim Biophys Acta. 2016 Jul 21. pii:

    Insert

    glycophorin A
    

    Expression

    Bacterial

    Available Since

    May 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a human Tankyrase2 full length Y920E

    Plasmid

    #132639

    Purpose

    expresses human Tankyrase2 full length Y920E in E. coli

    Depositor

    Wenqing Xu

    Article

    DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

    Insert

    Tankyrase2 Y920E
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET-6xHis-(pos16)GFP

    Plasmid

    #89249

    Purpose

    Expresses GFP with +16 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET28a-Rx3(A7)

    Plasmid

    #35525

    Depositor

    Robert Cohen

    Article

    Sims et al Nat Methods. 2012 Feb 5. doi: 10.1038/nm

    Insert

    His6-Rx3(A7)
    

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Changed G2 to P

    Promoter

    T7

    Available Since

    April 5, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-FIP200 (1-640)-TSF

    Plasmid

    #203545

    Purpose

    FIP200 NTD-TwinStrep-Flag mammalian expression

    Depositor

    James Hurley

    Article

    Chen et al bioRxiv 2023.06.01.543278

    Insert

    FIP200 (RB1CC1 Human)
    

    Tags

    TwinStrep Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJL030

    Plasmid

    #53533

    Purpose

    supplies EtpC required for optimal EtpA expression on compatible plasmid

    Depositor

    James Fleckenstein

    Article

    Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

    Insert

    etpC
    

    Use

    Pacyc184

    Expression

    Bacterial

    Available Since

    June 10, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pLNC Wnt-5bHA

    Plasmid

    #18034

    Depositor

    Jan Kitajewski

    Article

    Shimizu et al Cell Growth Differ. 1997 Dec . 8(12):134

    Insert

    Wnt-5b (Wnt5b Mouse)
    

    Use

    Retroviral

    Tags

    HA

    Available Since

    July 7, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pSNAP-H3 Zeo

    Plasmid

    #132427

    Purpose

    Expressing SNAP-tagged histone H3.1 in mammalian cells

    Depositor

    John M. Greally

    Article

    Sato et al J Cell Biol. 2019 Jun 3;218(6):1810-1823

    Insert

    Histone H3.1
    

    Expression

    Mammalian

    Available Since

    Oct. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMAL-c5X-ELP[AV-60]

    Plasmid

    #67012

    Purpose

    Expresses malE-ELP[AV-60] in E. coli

    Depositor

    Ashutosh Chilkoti

    Article

    Tang et al Nat Mater. 2016 Jan 4. doi: 10.1038/nmat

    Insert

    malE-ELP[AV-60]
    

    Tags

    His6 and malE-Factor Xa

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Jan. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GPI-mNeonGreen

    Plasmid

    #197912

    Purpose

    Monomer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

    Insert

    glycosylphosphatidylinositol-mNeonGreen
    

    Expression

    Mammalian

    Mutation

    none

    Available Since

    April 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pCS2 GSK3-GFP

    Plasmid

    #29680

    Depositor

    Edward De Robertis

    Article

    Taelman et al Cell. 2010 Dec 23. 143(7):1136-48.

    Insert

    glycogen synthase kinase 3
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 7, 2011

    Availability

    Academic Institutions and Nonprofits only

23. PT7lacO-EF-Ts-His6

    Plasmid

    #224371

    Purpose

    Express EF-Ts protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    EF-Ts (tsf E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. PT7lacO-EF-G-His8

    Plasmid

    #224369

    Purpose

    Express EF-G protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    EF-G (fusA E. coli)
    

    Tags

    His8

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. PT7lacO-His6-ThrRS

    Plasmid

    #224365

    Purpose

    Express ThrRS protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    ThrRS (thrS E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. PT7lacO-His6-CKM

    Plasmid

    #224375

    Purpose

    Express CKM protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    CKM (LOC107051134 Chicken)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. PT7lacO-His6-NDK

    Plasmid

    #224376

    Purpose

    Express NDK protein for his-tagged purification

    Depositor

    Richard Murray

    Article

    Zhang et al bioRxiv 2024.06.19.599772

    Insert

    NDK (ndk E. coli)
    

    Tags

    His6

    Expression

    Bacterial

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV mouse sidekick1

    Plasmid

    #18734

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Nature. 2008 Jan 24. 451(7177):465-9.

    Insert

    Sidekick-1 (Sdk1 Mouse)
    

    Expression

    Mammalian

    Available Since

    Aug. 15, 2008

    Availability

    Academic Institutions and Nonprofits only

29. mPA-GFP-Actin-C-18

    Plasmid

    #57121

    Purpose

    Localization: Actin, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Actin (ACTB Human)
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

30. mPA-GFP-Calnexin-N-14

    Plasmid

    #57122

    Purpose

    Localization: Endoplasmic Reticulum, Excitation: 400 / 504, Emission: 515 / 517

    Depositor

    Michael Davidson

    Insert

    Calnexin (CANX Human)
    

    Tags

    mPA-GFP

    Expression

    Mammalian

    Mutation

    I474N in Calnexin

    Promoter

    CMV

    Available Since

    Jan. 29, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

31. pET-6xHis-(pos14)GFP

    Plasmid

    #89252

    Purpose

    Expresses GFP with +14 theoretical charge in E.Coli

    Depositor

    David Liu

    Article

    Thompson et al Chem Biol. 2012 Jul 27;19(7):831-43. doi

    Insert

    His6-(pos16)-GFP
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGEX6P-1-Dendra2

    Plasmid

    #82436

    Purpose

    Dendra2 expression plasmid

    Depositor

    Periklis Pantazis

    Article

    Mohr et al Nat Protoc. 2016 Dec;11(12):2419-2431. d

    Insert

    Dendra2
    

    Tags

    GST tag

    Expression

    Bacterial

    Promoter

    tac

    Available Since

    Sept. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pGL0_7 [pSa ORI]

    Plasmid

    #198923

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    pSa ORI
    

    Use

    Synthetic Biology

    Available Since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pGL0_65 [StayGold]

    Plasmid

    #198976

    Purpose

    Level 0 part

    Depositor

    Cultivarium Tools, Nili Ostrov, Charlie Gilbert

    Article

    Gilbert et al bioRxiv 2023.05.19.541510

    Insert

    StayGold
    

    Use

    Synthetic Biology

    Available Since

    July 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-EPN-24

    Plasmid

    #79939

    Purpose

    PH-Late1-I3-01-myc

    Depositor

    Neil King

    Article

    Votteler et al Nature. 2016 Dec 8;540(7632):292-295. do

    Insert

    EPN-24
    

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pDRF1-GW ymScarletI-T2A-mTurquoise2

    Plasmid

    #118441

    Purpose

    Produces equimolar expression of yeast codon-optimized mScarletI and mTq2

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI-T2A-mTurquoise2
    

    Expression

    Yeast

    Available Since

    Feb. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pDL1403

    Plasmid

    #226635

    Purpose

    This plasmid induces natural competence in classical biotype Vibrio cholerae and expresses the genes tfox and qstR.

    Depositor

    Andrew Camilli

    Article

    Woldetsadik et al mBio. 2024 Oct 8:e0245724. doi: 10.1128/

    Inserts

    tfox
    qstR
    

    Available Since

    Jan. 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pEPQD0CM0296

    Plasmid

    #162305

    Purpose

    Level 0 CDS part for MoClo assembly, AATG-TTCG

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Insert

    CDS, superfolder GFP, no stop codon
    

    Use

    Synthetic Biology

    Available Since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pLSSmKate2-C1

    Plasmid

    #31869

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate2
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V compared to mK…

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pcDNA3.1 Orai3

    Plasmid

    #16370

    Depositor

    Anjana Rao

    Article

    Gwack et al J Biol Chem. 2007 Jun 1. 282(22):16232-4

    Insert

    Orai3 (ORAI3 Human)
    

    Tags

    His and Myc

    Expression

    Mammalian

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only