19,654 results

1. pBGPa-CMV-GFP-OSBP PH domain

   Plasmid

   #58724

   Purpose

   Expresses GFP-Tagged OSBP PH domain in mammalian cells

   Depositor

   Tim Levine

   Article

   Levine et al Curr Biol. 2002 Apr 30;12(9):695-704.

   Insert

   Oxysterol-binding Protein PH domain (OSBP Human)
   

   Tags

   GFP and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 27, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pCdrA-gfpC

   Plasmid

   #111614

   Purpose

   Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

   Depositor

   Tim Tolker-Nielsen

   Article

   Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

   Insert

   PcdrA-RBSII-gfp(Mut3)-T0-T1
   

   Expression

   Bacterial

   Available Since

   June 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pFA6a-link-ymNeongreen-URA3

   Plasmid

   #168059

   Purpose

   Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP

   Depositor

   Bas Teusink

   Article

   Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

   Insert

   ymNeonGreen
   

   Tags

   ymNeonGreen

   Expression

   Yeast

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRK7

   Plasmid

   #10883

   Depositor

   John Blenis

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Feb. 13, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCoPURO

   Plasmid

   #17533

   Depositor

   Francis Castellino

   Article

   Iwaki et al Biotechniques. 2003 Sep . 35(3):482-4, 4

   Insert

   Puromycine Acetyl Transferase
   

   Expression

   Insect

   Available Since

   March 21, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGST-PSP-hTG

   Plasmid

   #85470

   Purpose

   bacterial expression of human tissue transglutaminase

   Depositor

   Jeffrey Keillor, Ray Truant

   Article

   Roy et al Protein Expr Purif. 2013 Jan;87(1):41-6.

   Insert

   hTG2 (TGM2 Human)
   

   Tags

   GST and PSP

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   Nov. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV-GFP/Cre

   Plasmid

   #49056

   Purpose

   Adeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.

   Depositor

   Fred Gage

   Article

   Kaspar et al Proc Natl Acad Sci U S A. 2002 Feb 19;99

   Insert

   Cre
   

   Use

   AAV, Cre/Lox, and Mouse Targeting

   Tags

   EGFP, Myc, and NLS

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 13, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1_ADAR2DD_E488Q

   Plasmid

   #205598

   Purpose

   expresses human ADAR2 deaminase domain (E488Q hyperactive mutant) in mammalian cells

   Depositor

   Marc Friedlander

   Article

   Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

   Insert

   ADAR2 deaminase domain (ADARB1 Human)
   

   Expression

   Mammalian

   Mutation

   E488Q hyperactive mutation

   Available Since

   Oct. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTS96 pHAGE2 CMVtetO2 GFP-22xGS-SUMOEu-MCS

   Plasmid

   #199349

   Purpose

   Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-SUMOEu-tagged protein in mammalian cells.

   Depositor

   Rebecca Voorhees

   Article

   Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP-SUMOEu

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   April 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.0 FLAG TRPV4

    Plasmid

    #45751

    Depositor

    Robert Lefkowitz

    Article

    Shukla et al J Biol Chem. 2010 Sep 24;285(39):30115-2

    Insert

    TRPV4 (Trpv4 Rat)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. Artichoke

    Plasmid

    #73320

    Purpose

    Artichoke is a protein degradation reporter plasmid in a lentiviral backbone (SFFV.BsmBIClonesite.17AARigidLinker-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

    Depositor

    Benjamin Ebert

    Article

    Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    SFFV and EF1a

    Available Since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. Mm-PylRS-AF/Pyl-tRNACUA

    Plasmid

    #122650

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of unnatural amino acid into proteins in mammalian cells in response to the amber codon TAG.

    Depositor

    Howard Hang

    Article

    Spence et al Nat Chem Biol. 2019 Jan 14. pii: 10.1038

    Insert

    PylRS-AF, Pyl-tRNACUA
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Tyrosine 306 changed to Alanine, Tyrosine 384 cha…

    Available Since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.2 V5-DEST 3XFlag-SRSF3

    Plasmid

    #46736

    Depositor

    David Bartel

    Article

    Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

    Insert

    SRSF3 (SRSF3 Human)
    

    Tags

    3x Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C3-Exo70

    Plasmid

    #53761

    Purpose

    pEGFP-C3 plasmid that expresses Exo70

    Depositor

    Channing Der

    Article

    Martin et al Mol Cell. 2014 Jan 23;53(2):209-20. doi:

    Insert

    Exo70 (EXOC7 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAAV-synapsin-HaloCaMP1a-EGFP

    Plasmid

    #138327

    Purpose

    chemigenetic calcium indicator

    Depositor

    Eric Schreiter

    Article

    Deo et al Nat Chem Biol. 2021 Jun;17(6):718-723. d

    Has Service

    AAV1 and AAV9

    Insert

    HaloCaMP1a
    

    Use

    AAV

    Tags

    EGFP, His-tag, and Nuclear Exclusion Signal

    Expression

    Mammalian

    Promoter

    synapsin

    Available Since

    March 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Spectrin-M-cpstFRET

    Plasmid

    #61109

    Purpose

    Expression of Spectrin-cpstFRET to measure mechanical stress and report multidimensional stress fields

    Depositor

    Fred Sachs

    Article

    Meng et al J Cell Sci. 2012 Feb 1;125(Pt 3):743-50.

    Insert

    spectrin-cpstFRET
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMK265 (RAD21-mAC Hygro)

    Plasmid

    #140539

    Purpose

    RAD21 tagging with mAID-Clover

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. Tol2-elavl3-GCaMP6s

    Plasmid

    #59531

    Purpose

    Expressed GCaMP6-slow, a calcium indicator, in neurons in zebrafish larva. The plasmid is in Tol2 transposon backbone

    Depositor

    Misha Ahrens

    Article

    Vladimirov et al Nat Methods. 2014 Jul 27. doi: 10.1038/n

    Inserts

    elavl3 promoter (elavl3 Zebrafish)
    GCaMP6s
    

    Available Since

    Sept. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHIV-ODD-EGFP-IRES-dTomato

    Plasmid

    #87218

    Purpose

    lentiviral expression of ODD-EGFP

    Depositor

    Violaine Sée

    Article

    Bagnall et al J Biol Chem. 2014 Feb 28;289(9):5549-64.

    Insert

    HIF-1a ODD domain (HIF1A Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ODD domain

    Promoter

    EF1

    Available Since

    March 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pME-Cas9-T2A-GFP

    Plasmid

    #63155

    Purpose

    Middle Entry clone for Gateway containing a zebrafish codon-optimized Cas9 flanked by 2 NLS and followed by inframe GFP. Cas9 and GFP are separated by the sequence of a T2A self-cleaving peptide.

    Depositor

    Leonard Zon

    Article

    Ablain et al Dev Cell. 2015 Mar 4. pii: S1534-5807(15

    Insert

    Cas9-T2A-GFP
    

    Use

    CRISPR; Tol2 middle entry vector for gateway

    Available Since

    April 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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