We narrowed to 18,127 results for: Mut

1. pBP-L1U1H09

   Plasmid

   #73008

   Purpose

   L1U1H09 terminator

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   L1U1H09 terminator
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Promoter

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pICSL50016

   Plasmid

   #50315

   Depositor

   Nicola Patron

   Article

   Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

   Insert

   CDS, variant of turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A.
   

   Use

   Plant expression

   Tags

   Expression

   Mutation

   Codon-optimised for A. thaliana. BsaI/BbsI sites …

   Promoter

   Available Since

   Feb. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLenti-Arch-EEN

   Plasmid

   #45189

   Purpose

   Arch voltage indicator with double mutations D95N-D106E (Arch-EEN), with faster kinetics and greater fluorescence dynamic range than Arch-D95N

   Depositor

   Mark Schnitzer

   Article

   Gong et al PLoS One. 2013 Jun 19;8(6):e66959.

   Insert

   enhanced Archaerhodopsin-3
   

   Use

   Lentiviral

   Tags

   eYFP

   Expression

   Mammalian

   Mutation

   D95N, D106E

   Promoter

   CamKIIa

   Available Since

   July 1, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJC175e-DddI

   Plasmid

   #179693

   Purpose

   Mutagenesis plasmid with constitutive bacterial expression of DddI immunity protein

   Depositor

   David Liu

   Article

   Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

   Insert

   [SD8] gIII
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pReporter_11

   Plasmid

   #60571

   Purpose

   A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

   Depositor

   Christopher Voigt

   Article

   Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

   Insert

   Reporter_11
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   BBa_J23119

   Available Since

   March 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pUC19-U6-AagRNA

   Plasmid

   #121953

   Purpose

   Mammalian expression, Genome editing, gRNA scaffold

   Depositor

   Wei Li

   Article

   Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

   Insert

   AaCas12b tracrRNA/crRNA duplex
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. WDR5

   Plasmid

   #36900

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   WDR5 (WDR5 Human)
   

   Use

   Tags

   His tag with TEV cleavage

   Expression

   Bacterial

   Mutation

   mutation version of plasmid #60121 3UR4

   Promoter

   Available Since

   Aug. 8, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pSARSF505

   Plasmid

   #46832

   Depositor

   Hideo Iwai

   Article

   Aranko et al Nat Chem Biol. 2013 Aug 25. doi: 10.1038

   Insert

   GFPN-NpuDnaE_intein-GFP_C
   

   Use

   Tags

   GFP_C and H6-GFP_N

   Expression

   Bacterial

   Mutation

   stemmer GFP with superfolder mutations S30R and Y…

   Promoter

   T7

   Available Since

   Sept. 13, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CyclinB-GFP

   Plasmid

   #54465

   Purpose

   CyclinB-GFP in pIVT vector for in vitro transcription

   Depositor

   Michael Lampson

   Article

   Davydenko et al J Cell Biol. 2013 Jul 22;202(2):221-9. d

   Insert

   CyclinB
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   K67E and I150T mutations compared to GenBank refe…

   Promoter

   Available Since

   Jan. 13, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pTK64_GFP-NuMA-Bonsai

    Plasmid

    #46351

    Depositor

    Iain Cheeseman

    Article

    Kiyomitsu et al Cell. 2013 Jul 18;154(2):391-402. doi: 1

    Insert

    NuMA deleted 414-1549 (NUMA1 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    deleted amino acid 414-1549; L1589F mutation in N…

    Promoter

    pBABE

    Available Since

    Aug. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCMV-PTEN-H123Y

    Plasmid

    #28299

    Depositor

    Maria-Magdalena Georgescu

    Article

    Takahashi et al EMBO J. 2006 Feb 22. 25(4):910-20.

    Insert

    PTEN (PTEN Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Phosphatase Inactive Mutant His123Tyr

    Promoter

    Available Since

    Sept. 14, 2011

    Availability

    Academic Institutions and Nonprofits only

12. hGR-Dx

    Plasmid

    #133408

    Purpose

    overexpression of a mutant human glucocorticoid receptor in mammalian cells

    Depositor

    Andrew Cato

    Article

    Heck et al EMBO J. 1994 Sep 1;13(17):4087-95.

    Insert

    NR3C1 (NR3C1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    N454D, A458T, R460D, D462C

    Promoter

    Available Since

    Dec. 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-FRB-LplA(W37I)

    Plasmid

    #34840

    Depositor

    Alice Ting

    Article

    Slavoff et al J Am Chem Soc. 2011 Dec 14;133(49):19769

    Insert

    FRB-LplA(W37I)
    

    Use

    Tags

    FLAG (internal fusion), HA, and His Tag

    Expression

    Mammalian

    Mutation

    LplA has Trp37 to Val mutation

    Promoter

    CMV

    Available Since

    Feb. 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. IZ1181

    Plasmid

    #21049

    Depositor

    Tadahide Izumi

    Article

    Busso et al Oncogene. 2009 Apr 2. 28(13):1616-25.

    Insert

    human MDM2 (MDM2 Human)
    

    Use

    Tags

    no tag

    Expression

    Mammalian

    Mutation

    no mutation. This is the wild-type human MDM2.

    Promoter

    Available Since

    July 9, 2009

    Availability

    Academic Institutions and Nonprofits only

15. Flag-TRIM24 shRescue

    Plasmid

    #28139

    Depositor

    Michelle Barton

    Article

    Allton et al Proc Natl Acad Sci U S A. 2009 Jul 14. 1

    Insert

    TRIM24 (TRIM24 Human)
    

    Use

    Tags

    FLAGX2

    Expression

    Mammalian

    Mutation

    A489V; aa 308 and 309 mutated for shRNA rescue

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

16. MBP-TEV-VMA Model vector

    Plasmid

    #86590

    Purpose

    This can be used to make MBP-TEV-Cterm with SapI and PstI; and can also make N-term-VMA with NdeI and SapI

    Depositor

    Sharon Rozovsky

    Article

    Liu et al J Am Chem Soc. 2017 Feb 10. doi: 10.1021

    Insert

    VMA intein
    

    Use

    Tags

    His6-MBP

    Expression

    Bacterial

    Mutation

    Original SapI site from pMal was mutated out

    Promoter

    Tac

    Available Since

    Feb. 24, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pReporter_10

    Plasmid

    #60570

    Purpose

    A reporter plasmid containing the attB and attP sites flanking a green fluorescent protein reporter gene (gfp).

    Depositor

    Christopher Voigt

    Article

    Yang et al Nat Methods. 2014 Dec;11(12):1261-6. doi

    Insert

    Reporter_10
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    BBa_J23119

    Available Since

    March 16, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAGGS_2paCRY2(D387A)

    Plasmid

    #129652

    Purpose

    2P-activatable CRY2 with the FAD-disrupting mutation

    Depositor

    Michiyuki Matsuda

    Article

    Kinjo et al Nat Methods. 2019 Sep 9. pii: 10.1038/s4

    Insert

    2paCRY2(D387A)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSec7-Halox2

    Plasmid

    #115425

    Purpose

    Express Sec7-Halox2 at the endogenous locus via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    Sec7-Halox2
    

    Use

    Tags

    HaloTag

    Expression

    Yeast

    Mutation

    Promoter

    Endogenous

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pED7x26

    Plasmid

    #134813

    Purpose

    Stringent phage propagation reporter containing pIII-16-29-34-TAGA

    Depositor

    Ahmed Badran

    Article

    DeBenedictis et al Nat Commun. 2021 Sep 29;12(1):5706. doi:

    Insert

    pIII 16-TAGA 29-TAGA 34-TAGA
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    16-TAGA 29-TAGA 34-TAGA

    Promoter

    Available Since

    Sept. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pTU1-A-lacZ

    Plasmid

    #72935

    Purpose

    Level 1 Destination vector - Position A

    Depositor

    Paul Freemont

    Article

    Moore et al ACS Synth Biol. 2016 May 2.

    Insert

    Level 1 - A site
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Beta-lactamase - G717C (nucleotide) - silent muta…

    Promoter

    Available Since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pICH42222

    Plasmid

    #50328

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation; See De…

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pENTR-L5-CAG-R3

    Plasmid

    #141036

    Purpose

    Mutisite Gateway mediated vector construction

    Depositor

    Hideyuki Okano

    Article

    Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

    Insert

    CAG promoter
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. M-R742X (BF7-2)

    Plasmid

    #21372

    Depositor

    Gregory Germino

    Article

    Hanaoka et al Nature. . 408(6815):990-4.

    Insert

    autosomal dominant polycystic kidney disease type II (PKD2 Human)
    

    Use

    Tags

    6xMyc

    Expression

    Mammalian

    Mutation

    changed R742 to stop codon

    Promoter

    Available Since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

25. YIplac211-vps10-104

    Plasmid

    #115426

    Purpose

    Generate the vps10-104 allele via pop-in pop-out mutagenesis

    Depositor

    Benjamin Glick

    Article

    Casler et al J Cell Biol. 2019 May 6;218(5):1582-1601

    Insert

    vps10-104
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    domain 1 deletion

    Promoter

    Endogenous

    Available Since

    Nov. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNA-Wnt9A-V5

    Plasmid

    #35937

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt9A (WNT9A Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation A (543) --> G

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA-Wnt6-V5

    Plasmid

    #35932

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt6 (WNT6 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Silent Mutation G (567) --> A

    Promoter

    CMV

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Wnt9A STOP

    Plasmid

    #35881

    Depositor

    Marian Waterman

    Article

    Najdi et al Differentiation. 2012 Sep;84(2):203-13.

    Insert

    Wnt9A (WNT9A Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Silent Mutation A (543) --> G

    Promoter

    Available Since

    May 7, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCDNA HA FAK Y577F

    Plasmid

    #50507

    Depositor

    Kenneth Yamada

    Article

    Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

    Insert

    protein tyrosine kinase, mutated
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Y577F

    Promoter

    CMV

    Available Since

    Jan. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

30. UbG75C

    Plasmid

    #37194

    Depositor

    Cynthia Wolberger

    Article

    Wiener et al Nature. 2012 Feb 22;483(7391):618-22. do

    Insert

    Ubiquitin G75C mutant (RBX1 Human)
    

    Use

    Tags

    No tag

    Expression

    Bacterial

    Mutation

    Glycine 75 changed to Cystine

    Promoter

    T7

    Available Since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

31. pAC-EsaR-I70V

    Plasmid

    #47663

    Purpose

    pAC-EsaR with a208g mutation in esaR

    Depositor

    Cynthia Collins

    Article

    Shong et al ACS Chem Biol. 2013 Apr 19;8(4):789-95.

    Insert

    esaR-I70V
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Changed Isoleucine 70 to Valine

    Promoter

    Plac

    Available Since

    Sept. 6, 2013

    Availability

    Academic Institutions and Nonprofits only

32. YIp211-P30-His-pol30(K164R)-3'

    Plasmid

    #99548

    Purpose

    Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K164R; URA3 marker

    Depositor

    Helle Ulrich

    Article

    Davies et al Methods Mol Biol. 2012;920:543-67. doi:

    Insert

    POL30 (POL30 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Yeast

    Mutation

    K164R

    Promoter

    POL30

    Available Since

    Sept. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pICSL70005

    Plasmid

    #50335

    Depositor

    Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pDH6

    Plasmid

    #83777

    Purpose

    Useful for tagging yeast proteins with C-terminal YFP tag; G418 selection

    Depositor

    Eric Muller

    Article

    Hailey et al Methods Enzymol. 2002;351:34-49.

    Insert

    YFP
    

    Use

    Tags

    YFP

    Expression

    Yeast

    Mutation

    S65G, V68L, Q69K, S72A, T203Y mutations added.

    Promoter

    Available Since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAGGS-hCD4-GCaMP2

    Plasmid

    #18932

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    hCD4-GCaMP2 (CD4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

36. SNAP-Paxillin

    Plasmid

    #186143

    Purpose

    For expresseion of recombinant paxillin in bacteria

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Paxillin C1997A (silent mutation for cloning)

    Promoter

    Lac

    Available Since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. nonphosphorylatable Cenp-B-targted Aurora B FRET sensor

    Plasmid

    #45230

    Depositor

    Michael Lampson

    Article

    Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

    Insert

    nonphos Cenp-B targeted Aurora B FRET sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    t-a mutation in substrate sequence

    Promoter

    Available Since

    July 16, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pICH78133

    Plasmid

    #50292

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    5'UTR, omega (Tobacco Mosaic Virus) + chloroplast transit peptide, RbcS (synthetic)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. tdEos-Paxillin-KM-C-10

    Plasmid

    #57654

    Purpose

    Localization: Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

    Depositor

    Michael Davidson

    Article

    Kanchanawong et al Nature. 2010 Nov 25;468(7323):580-4. doi

    Insert

    Paxillin KM
    

    Use

    Tags

    tdEos

    Expression

    Mammalian

    Mutation

    Mutation to remove Kozak sequence

    Promoter

    CMV

    Available Since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pICH44300

    Plasmid

    #50340

    Depositor

    Sylvestre Marillonnet, Nicola Patron

    Article

    Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

    Insert

    3'UTR, polyadenylation signal/terminator, act2 (A. thaliana)
    

    Use

    Plant expression

    Tags

    Expression

    Mutation

    BsaI/BbsI sites removed by point-mutation

    Promoter

    Available Since

    Feb. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge