We narrowed to 2,657 results for: cmv promoter

1. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

   Plasmid

   #217804

   Purpose

   Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-linker-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-Phi29(+exo) (LM2938)

   Plasmid

   #208957

   Purpose

   A variant CE1 construct with Phi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-Phi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A)

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Polymerase variant click editor (CE1) - pCMV-T7-PCV2-nCas9-ePhi29(+exo) (LM2990)

   Plasmid

   #208958

   Purpose

   A variant CE1 construct with ePhi29 DNA polymerase (exo active), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-XTEN-nSpCas9-BPNLS-ePhi29(+exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); ePhi29(M8R/V51A/M97T/G197D/E221K/…

   Promoter

   CMV and T7

   Available since

   Nov. 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. Unfused click editor construct; PCV2-ePhi29 fusion - pCMV-T7-PCV2-ePhi29 (JO1420)

   Plasmid

   #217805

   Purpose

   Unfused click editor construct expressing PCV2-ePhi29(D169A), expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-linker-ePhi29-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   Phi29(D169A/M8R/V51A/M97T/G197D/E221K/Q497P/K512E…

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. Click editor utilizing mSA for template recruitment - pCMV-T7-mSA-nCas9-EcKlenow (JO1391)

   Plasmid

   #217802

   Purpose

   Variant CE1 construct with mSA for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Unfused click editor construct; mSA-EcKlenow fusion - pCMV-T7-mSA-EcKlenow (JO1401)

   Plasmid

   #217806

   Purpose

   Unfused click editor construct expressing mSA-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   mSA-linker-SV40NLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. Click editor utilizing Cdc13 for template recruitment - pCMV-T7-Cdc13-nCas9-EcKlenow (JO1293)

   Plasmid

   #217796

   Purpose

   Variant CE1 construct with Cdc13 telomere binding protein for template recruitment, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   Cdc13-XTEN-nSpCas9-BPNLS-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   nSpCas9(H840A); EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pR26-CMVconst

   Plasmid

   #127373

   Purpose

   Allows for constitutive gene expression from the murine ROSA26 safe harbor locus upon CRISPR/Cas9-mediated genomic insertion and stable selection.

   Depositor

   Lance Miller

   Article

   Routh et al Front Immunol. 2020 Feb 13;11:57. doi: 1

   Type

   Empty backbone

   Use

   CRISPR and Mouse Targeting

   Tags

   Expression

   Mutation

   Promoter

   CMV enhancer/promoter

   Available since

   Feb. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. MPRA_minCMV+GFP

   Plasmid

   #204866

   Purpose

   Vector containing GFP driven by minimal CMV promoter. This cassette can be cloned into MPRA vectors to serve as a reporter gene.

   Depositor

   Philipp Maass

   Article

   Oliveros et al Cell Genom. 2023 May 24;3(7):100330. doi

   Insert

   minCMV:GFP
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   minimal CMV

   Available since

   Sept. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJEP217-Lenti-CMV-GFP-WPRE-pA

    Plasmid

    #62925

    Purpose

    pLenti vector backbone designed to express GFP from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    Green Fluorescent protein (GFP)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    1.3αCaMKII and CMV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV:: NES-FKBP-iLID-LRP6c

    Plasmid

    #221774

    Purpose

    Expresses NES-FKBP-iLID-LRP6c component in mammalian cells

    Depositor

    Dongmin Lee

    Article

    Lee et al iScience. 2023 Feb 19;26(3):106233. doi:

    Insert

    pCMV:: NES-FKBP-iLID-LRP6c
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    June 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR/pSM2(CMV) GFP (w513-1)

    Plasmid

    #19170

    Purpose

    Gateway entry vector for miRNA expression under CMV promoter and GFP reporter

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    RNAi; Entry vector

    Tags

    GFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCBh_3x Flag-NLS-denOsCas12f1-NLS-VPR-NLS-mCherry_pA_phU6_gRNA scaffold-spacer_pCMV_BFP_pA

    Plasmid

    #197031

    Purpose

    Vector encoding a human codon-optimized dead enOsCas12f1 (denOsCas12f1)-VPR driven by CBh promoter, guide RNAs compatible with enOsCas12f1 driven by hU6, and BFP driven by CMV promoter

    Depositor

    Xiangfeng Kong

    Article

    Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

    Insert

    humanized denOsCas12f1-VPR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D52R / T132R / D228A / D406A

    Promoter

    CBh, CMV, hU6

    Available since

    May 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9(D10A)-BPNLS-P2A-EGFP (CA77)

    Plasmid

    #208290

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpCas9(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpCas9-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpCas9(D10…

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJEP226-Lenti-CMV-Flag-GluN2B-WRPE-pA

    Plasmid

    #84275

    Purpose

    pLenti vector backbone designed to express Flag-GluN2B from a CMV promoter.

    Depositor

    Jonathan Ploski

    Article

    Holehonnur et al Mol Brain. 2015 Feb 24;8:12. doi: 10.118

    Insert

    GluN2B
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mutation

    Promoter

    CMV

    Available since

    July 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-T7-nSpCas9(H840A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES140)

    Plasmid

    #185490

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(H840A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(H840A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    H840A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP (CA136)

    Plasmid

    #208291

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e(V106W) A-to-G base editor with SpRY(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e(V106W)-SpRY-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations and V106W in TadA and nSpRY(D10A/…

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCMV-T7-nSpCas9(D10A)-BPNLS(SV40)-3xFLAG-P2A-EGFP (HES24)

    Plasmid

    #185492

    Purpose

    CMV and T7 promoter expression plasmid for human codon usage nSpCas9(D10A) with a C-terminal bi-partite NLS, 3x FLAG, and P2A-eGFP

    Depositor

    Benjamin Kleinstiver, Raul Perez-Jimenez

    Article

    Alonso-Lerma et al Nat Microbiol. 2023 Jan;8(1):77-90. doi:

    Insert

    human codon usage nSpCas9(D10A)-BPNLS-P2A-eGFP
    

    Use

    In vitro transcription; t7 promoter

    Tags

    BPNLS-3xFLAG-P2A-eGFP

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CMV and T7

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV-CMV-Flag-Rat Arc-Arc- Genomic 3'UTR

    Plasmid

    #233053

    Purpose

    To express Flag-tagged Rat Arc from a CMV promoter. The Arc gene contains the Rat Arc native Genomic 3'UTR containing two introns

    Depositor

    Jonathan Ploski

    Insert

    Rat Arc (Arc Rat)
    

    Use

    AAV

    Tags

    Flag

    Expression

    Mutation

    Promoter

    Available since

    March 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. phCMV-coGALVenv-C4070A

    Plasmid

    #172217

    Purpose

    Expresses the envelope protein of Gibbon Ape Leukemia Virus (GALV) for pseudotyping of retroviral or lentiviral vectors

    Depositor

    Boris Fehse

    Article

    Mirow et al Viruses. 2021 Jul 27;13(8). pii: v130814

    Insert

    Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Carries C-terminus of amphotropic MLV strain 4070…

    Promoter

    CMV immediate early promoter

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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