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pGEX-2T_CVB1_VP1

Plasmid

#202069

Purpose

Bacterial expression of VP1 protein from coxsackievirus B1 (CVB1). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVB1 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX-2T_CVA4_VP1

Plasmid

#202068

Purpose

Bacterial expression of VP1 protein from coxsackievirus A4 (CAV4). Contains N-terminal GST-tag and C-terminal His6-tag.

Depositor

Vesa Hytönen

Article

Jouppila et al Microbiol Spectr. 2023 Jun 15;11(3):e053

Insert

CVA4 VP1 protein with Histag

Use

Tags

6xHis-tag and GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB2

Plasmid

#201533

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB2

Use

Synthetic Biology

Tags

Expression

Mutation

rhaR L128+1bp (frameshift), ilvA G455C

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJUMP24_T24_pRham-ilvA-relE_RBS3_CB4

Plasmid

#201535

Purpose

Expressing synthetic overlapping gene, ilvA-relE with internal RBS modification. Evolved strain with lower ilvA-relE expression. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Dan Park

Article

Chlebek et al Nucleic Acids Res. 2023 Jun 1:gkad484. d

Insert

ilvA-relE_RBS3_CB4

Use

Synthetic Biology

Tags

Expression

Mutation

rhaS ΔM1-H5, PrhaSR Δ179bp

Promoter

PrhaBAD (rhamnose)

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pEXPA10-NLS-3xmCitrine

Plasmid

#192523

Purpose

Fluorescent reporter for plant responses to bacterial infection

Depositor

Gitta Coaker

Article

Zhu et al Cell Rep. 2023 Jun 20;42(7):112676. doi:

Insert

EXPANSIN A10 (EXPA10 Mustard Weed)

Use

Tags

3xmCitrine and NLS

Expression

Plant

Mutation

Promoter

EXPA10

Available since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_beta-domain

Plasmid

#200300

Purpose

Bacterial expression plasmids for production of recombinant beta-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

beta-domain of metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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MT2A_alpha-domain

Plasmid

#200302

Purpose

Bacterial expression plasmids for production of recombinant alpha-domain of metallothionein 2a

Depositor

Artur Krężel

Article

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

alpha-domain of metallothionein 2a (MT2A Human)

Use

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Promoter

T7

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- BAD-YFP

Plasmid

#186567

Purpose

pMB1' ori, L-arabinose PBAD promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

BAD promoter

Available since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B-QHTKRKKtoAAAAAAA_AC

Plasmid

#148386

Purpose

Insect Expression of DmMe31B-QHTRKKtoAAAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-QHTRKKtoAAAAAA (me31B Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmMe31B-R307AK313AK314A_AC

Plasmid

#148387

Purpose

Insect Expression of DmMe31B-R307AK313AK314A

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

DmMe31B-R307AK313AK314A (me31B Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pSF-p15A-T7- YFP

Plasmid

#186568

Purpose

p15A ori, lacI PT7 promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Feb. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pKI-cjT-tdTomato-PGK-Neo

Plasmid

#186173

Purpose

Knock-in gene targeting in marmoset cells at TBXT locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjOSR1-EGFP-PGK-Puro

Plasmid

#186170

Purpose

Knock-in gene targeting in marmoset cells at OSR1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

EGFP

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Dec. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pKI-cjSF1-tdTomato-PGK-Neo

Plasmid

#186172

Purpose

Knock-in gene targeting in marmoset cells at SF1 locus

Depositor

Sho Yoshimatsu

Article

Yoshimatsu et al Biology (Basel). 2022 Jun 29;11(7):987.

Insert

tdTomato

Use

Marmoset targeting

Tags

Expression

Mutation

Promoter

Available since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- trc-YFP

Plasmid

#186565

Purpose

pMB1' ori, lacI, Ptrc promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

trc promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pSF-pMB1'- tac-YFP

Plasmid

#186566

Purpose

pMB1' ori, lacI, Ptac promoter, AmpR. Kringle YFP (Yellow Fluorescence Protein) expression

Depositor

Teresa de Diego Puente

Article

Lozano Terol et al Front Microbiol. 2021 Jun 21;12:682001.

Insert

YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

tac promoter

Available since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pNL(CMV)Rep78-III/CMV/WPREΔU3

Plasmid

#190726

Purpose

Expression of AAV2 Rep78-III in mammalian cells

Depositor

Jakob Reiser

Article

Li et al Hum Gene Ther Methods. 2018 Jun;29(3):13

Insert

AAV2 Rep78-III

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV-IE

Available since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160a

Plasmid

#173756

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160a_sgRNA2/R160a

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160b

Plasmid

#173757

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160b_sgRNA2/160b

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R390a

Plasmid

#173758

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/390a_sgRNA2/390a

Use

CRISPR

Tags

Expression

Mutation

Promoter

AtU6 &StU6

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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