170,819 results
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Plasmid#207353PurposeLentiviral transfer plasmid containing CMV-driven expression cassette encoding PEmax enzyme for prime editing.DepositorInsertPrime editor max (PEmax) enzyme
UseLentiviralAvailable SinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
lenti SYN-FLAG-dCas9-VPR
Plasmid#114196PurposeLentivirus compatible dCas9-VPR construct driven by the human SYN promoterDepositorInsertdCas9-VPR
UseLentiviralTagsFLAGExpressionMammalianPromoterSYNAvailable SinceSept. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJL357
Plasmid#243711PurposeA piggyBac vector containing the COX7A2 coding sequences, modified with silent mutations to allow for PCR-based analysis, along with endogenous promoter and UTRs.DepositorAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL361
Plasmid#243714PurposeA piggyBac vector containing a synthetic reporter containing the 5' and 3' UTRs of COX7A2 flanking a partial mCherry sequence with an intronDepositorInsert5' and 3' UTRs of COX7A2 flanking a partial mCherry sequence with an intron
ExpressionMammalianAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pJL358
Plasmid#243712PurposeA piggyBac vector containing the COX7A2 coding sequence (CDS) flanked by the 5' UTR of the COX7A2 gene and 3' UTR of the ACTB geneDepositorInsert5' UTR and CDS of COX7A2, followed by the 3' UTR of the ACTB gene (COX7A2 Human)
ExpressionMammalianPromoterendogenous promoterAvailable SinceNov. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAG_smFP FLAG
Plasmid#59756Purposeencodes multiple copies of an epitope tag FLAGDepositorInsertsmFP _FLAG
ExpressionMammalianPromoterCAGAvailable SinceOct. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pNL [NlucP/AP1-RE/Hygro] Vector
Plasmid#236811PurposeExpress AP1 response element in luciferase reporter assayDepositorHas ServiceDNAInsertAP1
UseLuciferaseTagsNanoLuc (R)MutationNonePromoterminPAvailable SinceJuly 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJ251-GERC
Plasmid#47441PurposeExpresses mCherry and sfGFP from divergent promoters. The backbone is cloned into DNA2.0 pJ251, and the design is based off of pZS2-123.DepositorInsertGene Expression Reporter Construct
ExpressionBacterialAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
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Klotho (secreted)
Plasmid#17713DepositorAvailable SinceApril 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pCFJ104 - Pmyo-3::mCherry::unc-54
Plasmid#19328DepositorArticleInsertPmyo-3::mCherry
TagsmCherryExpressionWormAvailable SinceNov. 21, 2008AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mNeon-Blast
Plasmid#207709PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Blast cassetteDepositorTypeEmpty backboneUseCRISPR; Donor cassetteExpressionMammalianAvailable SinceNov. 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPR-v2-HygR-EGFP
Plasmid#167188PurposeLentiviral expression of sgRNA with hygromycin resistance gene and EGFPDepositorInsertHygromycin B phosphotransferase and EGFP
UseCRISPR and LentiviralExpressionMammalianAvailable SinceOct. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAcBac1-NES-Flag-R2-84-MbRS Tetrazine3.0
Plasmid#174081PurposeExpression of orthogonal Mb tRNA and tRNA synthetase for incorporation of tetrazine3.0 in eukaryotic cells.DepositorInsertNES-Flag-R2-84 M. barkeri tRNA synthetase for tetrazine3.0
TagsNES-FlagExpressionMammalianMutationL270G, Y271L, N311G, C313APromoterCMVAvailable SinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2
Plasmid#220243PurposeExpresses wild-type EZH2 for knockout/rescue experiments in mammalian cells.DepositorAvailable SinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-TetO-hcMYC
Plasmid#206387PurposeExpresses human c-MYC in mammalian cells, for lentivirus generation.DepositorInsertc-MYC (MYC Human)
UseLentiviralAvailable SinceOct. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRS303H-Padh-OsTIR1(F74G)
Plasmid#247625PurposeExpression of OsTIR1DepositorInsertOsTIR1
ExpressionYeastMutationF74GPromoterADH1Available SinceJan. 2, 2026AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-CMV-Bamh1
Plasmid#203303PurposeFor expression of eukaryotic genes of interest with CMV promoter with Bamh1 as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
PlayBack-Ef1a-Nde1
Plasmid#203309PurposeFor expression of eukaryotic genes of interest with Ef1a promoter with NdeI as a PlayBack Restriction EnzymeDepositorTypeEmpty backboneExpressionMammalianAvailable SinceOct. 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET22b_PstS_2
Plasmid#78199PurposeExpression of double-cysteine variant of mature phosphate binding protein, PstS, as scaffold for phosphate biosensorDepositorInsertpstS (A17C,A197C)
ExpressionBacterialMutationA17C; A197C (please see depositor comment below)PromoterT7Available SinceJuly 11, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_CBFB_WT
Plasmid#81929PurposeGateway Donor vector containing CBFB, part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
TFORF0981
Plasmid#141778PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJan. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE26
Plasmid#90368PurposeC/EBPb - CEBPB gene reporterDepositorInsertFirefly luciferase
UseLentiviralPromoterCEBPbAvailable SinceAug. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLAS2w.Phyg-HAVCR1
Plasmid#232669Purposeexpresses HAVCR1 in mammalian cellsDepositorAvailable SinceMarch 7, 2025AvailabilityAcademic Institutions and Nonprofits only -
Raptor_2 shRNA
Plasmid#1858DepositorAvailable SinceApril 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
TL028_AAV_hSyn:HaloTag-GluA1
Plasmid#192520PurposeExpression of AMPA GluA1 with N terminal HaloTag fusionDepositorInsertHaloTag-GluA1
UseAAV and Cre/LoxTagsHaloTagPromoterhSynAvailable SinceJuly 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBad-LgBiT-PhoCl1-SmBiT-MBP
Plasmid#164034Purposeluciferase-based PhoCl screening constructDepositorInsertLgBiT-PhoCl1-SmBiT-MBP
TagsHis tag, T7 tag, Xpress TagExpressionBacterialPromoteraraBad promotorAvailable SinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-RIα-EGFP
Plasmid#181840PurposeGFP-tagged PKA RIα subunit for mammalian expression.DepositorAvailable SinceJune 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHIP_RNA2
Plasmid#61260PurposeOrsay virus RNA2 genome segment expression plasmid for virus reverse geneticsDepositorInsertOrsay RNA2
ExpressionWormPromoterheat shock promoterAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pDB5318
Plasmid#204828PurposeAn integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.DepositorInsertstetR
mECitrine
ExpressionYeastPromoterCMV promoter and enotetS promoterAvailable SinceAug. 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AAV-F in pAR-9
Plasmid#166921PurposeAAV rep/cap plasmid. Capsid is mouse brain tropic AAV-FDepositorInsertREP/CAP
UseAAVAvailable SinceApril 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pmiRGLO Dual-Luciferase-HNRNPAB-3'UTR
Plasmid#182249PurposeHuman HNRNPAB 3` UTR region containing 2x binding sites for miR-150 cloned downstream of luciferase reporter geneDepositorInsert3' UTR region of HNRNPAB gene
UseLuciferaseExpressionMammalianAvailable SinceMarch 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
TFORF0831
Plasmid#142208PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF0489
Plasmid#143029PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLVX-NPM1c-IRES-mCherry
Plasmid#131825PurposeLentiviral plasmid expressing NPM1 wildtype (cytoplasmic mutant, human) in mammalian cells upon transductionDepositorInsertNPM1 (NPM1 Human)
UseLentiviralAvailable SinceMay 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pdCas9
Plasmid#46569PurposeExpresses the tracrRNA, the dCas9 catalytic site mutant and a CRISPR array designed for the easy cloning of new spacers.DepositorInsertstracrRNA
dcas9
CRISPR array
UseCRISPRExpressionBacterialMutationD10A, H840AAvailable SinceAug. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
shp63alpha pLKO.1 puro
Plasmid#19120DepositorInsertsmall hairpin RNA against tumor protein p63 (TP63 Human)
UseLentiviral and RNAiExpressionMammalianAvailable SinceSept. 9, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
Tags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable SinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSetB-Peredox
Plasmid#73806PurposeExpresses the Peredox fluorescent sensor protein (for NADH/NAD ratio) in bacteria, with N-terminal his-tagDepositorInsertPeredox
Tagshis-tag to facilitate purificationExpressionBacterialPromoterT7Available SinceMarch 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCV006-ermg
Plasmid#232824PurposeRandom transposon mutagenesis vector for Bacteroidales species - erythromycinDepositorInsertermG
ExpressionBacterialAvailable SinceFeb. 27, 2025AvailabilityAcademic Institutions and Nonprofits only