We narrowed to 23,940 results for: nes

1. px330 p300 gRNA

   Plasmid

   #165591

   Purpose

   Insertion of p300 degron

   Depositor

   Cornelis Murre

   Article

   Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

   Insert

   P300 (Smarca2 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   NA

   Promoter

   Available since

   March 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCEP4 Smad2

   Plasmid

   #16484

   Depositor

   Bert Vogelstein

   Article

   Riggins et al Nat Genet. 1996 Jul . 13(3):347-9.

   Insert

   Smad2 (SMAD2 Human)
   

   Use

   Tags

   Double HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2008

   Availability

   Academic Institutions and Nonprofits only

3. HA-MBP-TEVpCS-ADGRE1 CTF-FLAG

   Plasmid

   #217689

   Purpose

   Mammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domains

   Depositor

   Stephen Blacklow

   Article

   Dates et al bioRxiv 2022.07.14.500097

   Insert

   ADGRE1 (EMR1 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Deletion of residues 1-584

   Promoter

   Available since

   April 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. HA-AMOT p130 deltaCC

   Plasmid

   #32827

   Depositor

   Kunliang Guan

   Article

   Zhao et al Genes Dev. 2011 Jan 1;25(1):51-63.

   Insert

   AMOT p130 (AMOT Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   E5D, G858D, deletion of amino acids 410-751

   Promoter

   CMV

   Available since

   Jan. 11, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-FBL6 delta6

   Plasmid

   #19925

   Depositor

   Yue Xiong

   Article

   Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

   Insert

   FBL6 delta 6 (FBXL6 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   delta 6

   Promoter

   Available since

   May 7, 2009

   Availability

   Academic Institutions and Nonprofits only

6. pENTR L1-vas2-Cre-L4

   Plasmid

   #62301

   Purpose

   Cre recombinase-expressing helper plasmid for Anopheles gene engineering, vasa promoter

   Depositor

   Eric Marois

   Article

   Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

   Insert

   Cre recombinase under control of vasa promoter and terminator
   

   Use

   Tags

   HA and SV40 NLS

   Expression

   Insect

   Mutation

   Promoter

   vasa

   Available since

   March 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5/FRT/TO HSPH1

   Plasmid

   #19453

   Depositor

   Harm Kampinga

   Article

   Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

   Insert

   HSPH1 (HSPH1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOD_A0508

   Plasmid

   #91014

   Purpose

   Module A, Promoter: EC1.2, Gene: Csy4-P2A-AtCas9, Terminator: HSP

   Depositor

   Daniel Voytas

   Article

   Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

   Insert

   Csy4-P2A-AtCas9
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   EC1.2

   Available since

   July 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGEMT-PT2A-Tdtomato-iRFP670-GFP

   Plasmid

   #111815

   Purpose

   Tricistronic construct: Co-express three fluorescent proteins by P2A and then T2A

   Depositor

   Li Qian

   Article

   Liu et al Sci Rep. 2017 May 19;7(1):2193. doi: 10.

   Inserts

   Tdtomato
   iRFP670
   GFP
   

   Use

   Cloning intermediate

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. SNV-gfp-IRES-MCS

    Plasmid

    #53122

    Purpose

    Retrovirus vector backbone that co-expresses GFP and a gene of interest, driven by the Spleen Necrosis Virus promoter; for use in chicken cells

    Depositor

    Jeanette Hyer

    Article

    Venters et al Dev Dyn. 2008 Nov;237(11):3260-9. doi: 1

    Type

    Empty backbone

    Use

    Retroviral; For expression in chicken cells

    Tags

    EGFP-IRES

    Expression

    Mutation

    Promoter

    SNV (spleen necrosis virus)

    Available since

    July 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ2370 - intron GG3 - 250bp

    Plasmid

    #159879

    Purpose

    Golden Gate compatible intron with PATCs for reduced germline silencing

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    intron GG3 - 250bp
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. Twist-siRNA5

    Plasmid

    #8456

    Depositor

    Bob Weinberg

    Article

    Yang et al Cell 2004 Jun 25;117(7):927-39.

    Insert

    siTwist (Twist1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 4, 2005

    Availability

    Academic Institutions and Nonprofits only

15. pV1539

    Plasmid

    #111434

    Purpose

    Genedrive entry vector for Candida albicans

    Depositor

    Gerald Fink

    Article

    Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

    Insert

    CaCas9/sgRNA-BsmBI stuffer
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3 5'cMyc-SMCl- S957A/S966A

    Plasmid

    #32372

    Depositor

    Michael Kastan

    Article

    Kim et al Genes Dev. 2002 Mar 1;16(5):560-70.

    Insert

    SMC1A (SMC1A Human)
    

    Use

    Tags

    c-Myc

    Expression

    Mammalian

    Mutation

    S957A/S996A

    Promoter

    Available since

    Sept. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

17. pEF1α-mouse-retroCHMP3-HA

    Plasmid

    #154178

    Purpose

    expresses mouse retroCHMP3 in mammalian cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

    Insert

    retroCHMP3
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. AB65_pCAG.Cas9-D10A.rBGpA

    Plasmid

    #199256

    Purpose

    Expression construct encoding the SpCas9-D10A nickase

    Depositor

    Manuel Goncalves

    Article

    Wang et al Nucleic Acids Res. 2021 Jan 25;49(2):117

    Insert

    SpCas9.D10A nickase
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A

    Promoter

    CAG promoter

    Available since

    April 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. Donor_eGP2AP_RC

    Plasmid

    #133784

    Purpose

    Library reporter vector/integration vector. Contains library cloning sites MluI and SpeI upstream of a terminator and downstream of a BxB1 attB site. Library integration occurs through BxB1 site.

    Depositor

    Sriram Kosuri

    Article

    Davis et al Cell Syst. 2020 Jul 22;11(1):75-85.e7. d

    Type

    Empty backbone

    Use

    Tags

    eGFP-P2A-Puro

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHSP70B-CP6-pac

    Plasmid

    #165877

    Purpose

    Basic Puromycin resistance cassette in Alpha1, lacks physical marker or attB site

    Depositor

    Koen Venken

    Article

    Matinyan et al Cell Rep. 2021 Sep 14;36(11):109700. doi

    Insert

    Basic Selection Vector
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pSR-puro-Exo84

    Plasmid

    #31116

    Depositor

    Christopher Counter

    Article

    Issaq et al Mol Cancer Res. 2010 Feb . 8(2):223-31.

    Insert

    Exo84 shRNA (EXOC8 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2011

    Availability

    Academic Institutions and Nonprofits only

22. pRU52

    Plasmid

    #167678

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pTH

    Plasmid

    #56659

    Purpose

    Dual-expression vector for P. tricornutum

    Depositor

    Philip Weyman

    Article

    Weyman et al Plant Biotechnol J. 2014 Oct 10. doi: 10

    Insert

    KanR
    

    Use

    Synthetic Biology; Diatom expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pISH-En2

    Plasmid

    #84310

    Purpose

    Use to synthesize riboprobes for in situ hybridization (ISH) probe for En2. Not for gene expression.

    Depositor

    Peter Mombaerts

    Article

    Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

    Insert

    En2 (En2 Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pNLRP11major.MYC

    Plasmid

    #131196

    Purpose

    Mammalian expression of human NLRP11 188S

    Depositor

    Thomas Kufer

    Article

    Ellwanger et al J Biol Chem. 2018 Jan 4. pii: RA117.0001

    Insert

    NLRP11 188S (NLRP11 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    188S

    Promoter

    pCMV

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcF-1

    Plasmid

    #131023

    Purpose

    Single spacer array with spacer #1 for FnCas12a.

    Depositor

    Chase Beisel

    Article

    Liao et al Nat Commun. 2019 Jul 3;10(1):2948. doi:

    Insert

    single spacer array for FnCas12a
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PJ23119

    Available since

    Sept. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pRK5-mFzd1-1D4

    Plasmid

    #42263

    Depositor

    Chris Garcia, Jeremy Nathans

    Article

    Yu et al Development. 2012 Dec 1;139(23):4383-94.

    Insert

    Frizzled 1 (Fzd1 Mouse)
    

    Use

    Tags

    1D4

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pMSCV-Pidd1(T788D)

    Plasmid

    #60530

    Purpose

    Retroviral expression of Pidd1 T788D phosphorylation mutant

    Depositor

    Trudy Oliver

    Article

    Terry et al Cell Death Differ. 2014 Oct 10. doi: 10.

    Insert

    Pidd1 (PIDD1 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Threonine 788 to Aspartic acid*

    Promoter

    Available since

    March 11, 2015

    Availability

    Academic Institutions and Nonprofits only

29. BII-ChPtW-PLAGL2

    Plasmid

    #133380

    Purpose

    Piggybac vector for constitutive expression

    Depositor

    Blair Madison

    Article

    Fischer et al Am J Physiol Gastrointest Liver Physiol.

    Insert

    PLAGL2 (PLAGL2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    CMV enhancer and hEf1a (CpG free)

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pENTR1A_no_ccDB_mUty

    Plasmid

    #158570

    Purpose

    V5-tagged sequence of mUty cloned into entry vector for recombination

    Depositor

    Christian Deschepper

    Article

    Deschepper et al Sci Rep. 2020 Sep 10;10(1):14900. doi: 1

    Insert

    Mus musculus ubiquitously transcribed tetratricopeptide repeat containing, Y-linked (Uty), transcript variant 2 (Uty Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    Available since

    Sept. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pOGG012

    Plasmid

    #113986

    Purpose

    Vector construction module. Partition genes (parABCDE) for plasmid stability in absence of antibiotic selection for construction of Golden Gate cloning vectors

    Depositor

    Philip Poole

    Article

    Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

    Insert

    Partition genes (parABCDE)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Domesticated for Golden Gate cloning

    Promoter

    Available since

    Jan. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. E12 2/5-pCS2

    Plasmid

    #20913

    Depositor

    Stephen Tapscott

    Article

    Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

    Insert

    E12 2/5 (TCF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 28, 2009

    Availability

    Academic Institutions and Nonprofits only

33. pLNHA-C1-HsUPF2-siRNAres_I

    Plasmid

    #146567

    Purpose

    Mammalian Expression of HsUPF2-siRNAres

    Depositor

    Elisa Izaurralde

    Article

    Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

    Insert

    HsUPF2-siRNAres (UPF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. PGL3-MK17

    Plasmid

    #21486

    Depositor

    Shoumo Bhattacharya

    Article

    Leung et al Genomics. 1999 Nov 1. 61(3):307-13.

    Insert

    Cited2 Promoter (CITED2 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 23, 2009

    Availability

    Academic Institutions and Nonprofits only

35. pCORE-Hp53

    Plasmid

    #72242

    Purpose

    Contains the CORE cassette with hyg and human p53 gene markers

    Depositor

    Francesca Storici, Michael Resnick

    Article

    Storici et al Genet Eng (N Y). 2003;25:189-207.

    Inserts

    p53 (TP53 Human)
    hyg
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    V122A mutation

    Promoter

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCngA2-ISH

    Plasmid

    #15855

    Depositor

    Peter Mombaerts

    Article

    Hirota et al Mol Cell Neurosci. 2007 Apr . 34(4):679-

    Insert

    Cyclic Nucleotide Gated Channel Alpha 2 (Cnga2 Mouse)
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 31, 2009

    Availability

    Academic Institutions and Nonprofits only

37. pCRISPR_eGFP_369

    Plasmid

    #111823

    Purpose

    Knockout eGFP

    Depositor

    Steve Shih

    Article

    Sinha et al Lab Chip. 2018 Jul 24;18(15):2300-2312.

    Insert

    gRNA targeting eGFP 349-369
    

    Use

    CRISPR

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    U6

    Available since

    Aug. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pRU51

    Plasmid

    #167677

    Purpose

    Final destination vector for single Gateway LR reaction

    Depositor

    Niko Geldner

    Article

    Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

    Insert

    PcUB4-2::spCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. gRNA-mCtnnb1

    Plasmid

    #84542

    Purpose

    mCtnnb1 gRNA expressing vector

    Depositor

    Bon-Kyoung Koo

    Article

    Andersson-Rolf et al Nat Methods. 2017 Jan 30. doi: 10.1038/n

    Insert

    mCtnnb1 gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pCAG-attBr-Cre-rc-attP

    Plasmid

    #51264

    Purpose

    Component of the Bxb1-rcCre binary site-specific recombinase system, reverse complementary Cre CDS flanked by Bxb1 attB/P recognition sites in head-to-head orientation

    Depositor

    Pawel Pelczar

    Article

    Hermann et al Nucleic Acids Res. 2014 Jan 9.

    Insert

    attBr-Cre-rc-attP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge