173,291 results

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pcDNA3-beta-HA-BRCA2

Plasmid

#133553

Purpose

Mammalian expression vector with CMV promoter driving beta globin intron, HA tag, BRCA2 ORF

Depositor

David Livingston

Article

Chen et al Mol Cell. 1998 Sep;2(3):317-28.

Insert

BRCA2 (BRCA2 Human)

Expression

Mammalian

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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MBD_R44Q-IFP2.0C

Plasmid

#215749

Purpose

Expresses binding-deficient R44Q mutant of MBD1 MBD domain fused to C-terminal part of IFP2.0 as negative control for 5mC detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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scFv-IFP2.0N

Plasmid

#215746

Purpose

Expresses single chain Fragment variable (scFv) fused to N-terminal part of split IFP2.0

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

IFP2.0N

Expression

Mammalian

Promoter

SFFV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightR2-control

Plasmid

#221675

Purpose

Expresses nLightR2-control in neurons

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods. 2026 Mar;23(3):636-652. doi

Insert

nLightR2-control

Use

AAV

Expression

Mammalian

Promoter

human Synapsin-1

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pDONR_221_middle-entry_NanoLuc

Plasmid

#128673

Purpose

NanoLuc CDS middle entry gateway clone

Depositor

Steven Farber

Article

Thierer et al Nat Commun. 2019 Jul 31;10(1):3426. doi:

Insert

NanoLuc

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a(+)-Histag-mCherry (527)

Plasmid

#70719

Purpose

Expression vector for mCherry purification

Depositor

Richard Murray

Article

Niederholtmeyer et al Elife. 2015 Oct 2;4. doi: 10.7554/eLife.

Insert

mCherry

Available Since

Feb. 2, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV-LACTB-dAPEX2

Plasmid

#117179

Purpose

Plasmid name in publication: pAAV-IMS-dAPEX2. Peroxidase reporter for multiplexed electron microscopy labeling

Depositor

David Ginty

Article

Zhang et al Nat Neurosci. 2019 Mar 18. pii: 10.1038/

Insert

LACTB-dAPEX2

Use

AAV

Expression

Mammalian

Mutation

W41F and A134P on soybean APX

Promoter

EF1a

Available Since

March 19, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5 bovine GRK2

Plasmid

#14691

Depositor

Robert Lefkowitz

Article

Pitcher et al J Biol Chem. 1999 Dec 3. 274(49):34531-4

Insert

GRK2 (GRK2 Bovine)

Expression

Mammalian

Available Since

June 19, 2007

Availability

Academic Institutions and Nonprofits only
pBC2103-reci

Plasmid

#117172

Purpose

U6-sgRNA-EFS-arC9-T2A-mCherry-P2A-Hygro; arC9 without NLS

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Insert

reci_arCas9 without NLS

Use

Lentiviral

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pHS2096

Plasmid

#239835

Purpose

NovaIscB E. coli expression for recombinant purification

Depositor

Feng Zhang

Article

Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

Insert

NovaIscB

Tags

14xHis-TwinStrep-bdSUMO

Expression

Bacterial

Mutation

Insertion of REC domain from NbaCas9-1 and swap o…

Promoter

T7

Available Since

July 21, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT/TO-3*Flag-APEX2 N-term RNPS1

Plasmid

#187574

Purpose

Express FLAG epitope and APEX2-tagged RNPS1 fusion protein in mammalian cells

Depositor

Benjamin Blencowe

Article

Barutcu et al Mol Cell. 2022 Mar 3;82(5):1035-1052.e9.

Insert

RNPS1 (RNPS1 Human)

Tags

APEX2-FLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti CMV Hygro DEST (w117-1)

Plasmid

#17454

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV promoter, Hygro

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Gateway Cloning and Lentiviral

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit HC

Plasmid

#221349

Purpose

Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit HC; to be used with Anti-Biotin [IPI-M33] light chain (Plasmid 221350) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Insert

Anti-Biotin [IPI-M33] Mouse/Rabbit HC

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pTipi2.1_Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

Plasmid

#221350

Purpose

Mammalian expression of Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa; to be used with Anti-Biotin [IPI-M33] heavy chain (Plasmid 221349) to make the antibody.

Depositor

Institute for Protein Innovation (IPI)

Article

Yang et al bioRxiv, March 20, 2026

Insert

Anti-Biotin [IPI-M33] Mouse/Rabbit LC kappa

Use

Affinity Reagent/ Antibody

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 1, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pRDB_270

Plasmid

#231111

Purpose

C>T base editor

Depositor

John Doench, David Root

Article

Kaplan et al Nat Genet. 2025 Nov;57(11):2920-2929. do

Insert

TadA-CDd-nCas9-SpG-UGI-HA

Use

CRISPR and Lentiviral

Mutation

D10A

Available Since

Jan. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pFUChW Parkin shRNA Mul1 shRNA

Plasmid

#78523

Purpose

Lentiviral vector encoding two shRNAs and mCherry reporter

Depositor

David Chan

Article

Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

Insert

Park2 shRNA, Mul1 shRNA (Prkn, Mul1 Mouse)

Use

Lentiviral and RNAi

Promoter

H1

Available Since

Jan. 5, 2017

Availability

Academic Institutions and Nonprofits only

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SLF063: Lenti-fCAG-jRGECO1a

Plasmid

#124775

Purpose

For general lentiviral or direct expression of cytosolic jRGECO1a

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Promoter

CAG

Available Since

June 4, 2019

Availability

Academic Institutions and Nonprofits only
Nano-lantern(cAMP-0.4)/pcDNA3

Plasmid

#53593

Purpose

Nano-lantern-based luminescent cAMP indicator

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern-based luminescent cAMP indicator

Expression

Mammalian

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only
CBP TAZ1(340-439)

Plasmid

#173760

Purpose

bacterial expression of mouse CBP TAZ1 domain

Depositor

Peter Wright

Article

De Guzman et al Biochemistry. 2005 Jan 18;44(2):490-7. d

Insert

CBP TAZ1 (Crebbp Mouse)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pFUW PuroR-P2A-COX8ASig-Timer

Plasmid

#239559

Purpose

Expression and mitohcondrial localization of Timer

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

Timer

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLX_313-EGFP

Plasmid

#158580

Purpose

Lentiviral expression of EGFP

Depositor

John Doench, David Root

Article

Hanna et al Cell. 2021 Feb 18;184(4):1064-1080.e20.

Insert

EGFP

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5myc Rac1 L61

Plasmid

#15903

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

Rac1 (RAC1 Human)

Tags

myc

Expression

Mammalian

Mutation

Q61L

Available Since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pCIneo-HA

Plasmid

#115360

Purpose

Cloning vector to produce HA-fusion proteins without any other gene insert

Depositor

Witold Filipowicz, Ramesh Pillai

Article

Pillai et al RNA. 2004 Oct . 10(10):1518-25.

Insert

HA

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pAAVdual-CMV-eGFP

Plasmid

#230931

Purpose

pAAVdual plasmid is used to package AAV-CMV-eGFP viruses with the AAVdual system, which integrates the mini-pHelper-1.0 (providing E2A, E4orf6, and VA RNA) with an AAV expression cassette containing eGFP driven by the CMV promoter.

Depositor

Qizhao Wang

Article

Yang et al Mol Ther Nucleic Acids. 2025 May 14;36(2

Insert

eGFP

Use

AAV

Promoter

CMV

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTH100

Plasmid

#84458

Purpose

pJB38-NWMN29-30 + SarA_P1-sGFP-Term

Depositor

Reindert Nijland

Article

de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

Insert

SarA_P1-sGFP from pCM29

Tags

6xHis (on sfGFP)

Available Since

Feb. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-Streptavidin-Alive

Plasmid

#20860

Purpose

Alive subunit for creating monovalent streptavidin with a single femtomolar biotin binding site. Can also be used to create wild-type (tetravalent) streptavidin.

Depositor

Alice Ting

Article

Howarth et al Nat Methods. 2006 Apr . 3(4):267-73.

Insert

Streptavidin-Alive

Tags

His

Expression

Bacterial

Mutation

Wt gene

Available Since

April 21, 2009

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAcT2-moxGFP2

Plasmid

#218966

Purpose

Expresses full length GalNAcT2-moxGFP2 mammalian cells

Depositor

Benjamin Glick

Insert

GalNAcT2-moxGFP2 (GALNT2 Human)

Tags

moxGFP2

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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10-4 cyclin E promoter

Plasmid

#8458

Depositor

Bob Weinberg

Article

Geng et al Oncogene 1996 Mar 21;12(6):1173-80.

Insert

human cyclin E promoter (CCNE1 Human)

Use

Luciferase

Expression

Mammalian

Available Since

July 28, 2006

Availability

Academic Institutions and Nonprofits only

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pAF-HdrB3

Plasmid

#184908

Purpose

carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

Depositor

Radhakrishnan Mahadevan

Article

Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

Insert

Cas9-sgRNA-homology arms

Use

CRISPR; Broad-host-range

Expression

Bacterial

Available Since

Sept. 7, 2022

Availability

Academic Institutions and Nonprofits only

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