We narrowed to 6,088 results for: pcDNA 3.1

1. pC1-IMS-oROS-HT

   Plasmid

   #216419

   Purpose

   Targeting of the chemigenetic, fluorescent peroxide sensor oROS-HT to the inter-membrane-space of mitochondria.

   Depositor

   Andre Berndt

   Article

   Lee et al bioRxiv 2024.02.06.579232

   Insert

   oROS-HT
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. miniABEMax V82G nSaCas9

   Plasmid

   #135365

   Purpose

   Expression of SaCas9 nickase (D10A) with single evolved TadA monomer (ABEMax) with a V82G substitution

   Depositor

   Alex Hewitt

   Article

   Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

   Insert

   miniABEMax V82G nSaCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   (V82G)

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. c-Myc-5-HT2A

   Plasmid

   #67944

   Purpose

   N-terminally c-myc tagged human 5-hydroxytryptamine receptor 2A

   Depositor

   Javier Gonzalez-Maeso

   Article

   Gonzalez-Maeso et al Nature. 2008 Mar 6;452(7183):93-7. doi:

   Insert

   HTR2A (HTR2A Human)
   

   Tags

   c-Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Sept. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CaMK2rep

   Plasmid

   #239622

   Purpose

   CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Article

   Martinez-Blanco et al Anal Chem. 2025 Sep 23;97(37):20275-2029

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CMV-CASANOVA

   Plasmid

   #113035

   Purpose

   CASANOVA (AcrIIA4-LOV2 hybrid) expression in mammalian cells; enables optogenetic control of SpyCas9

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   CASANOVA (for CRISPR/Cas9 activity switching via a novel optogenetic variant of AcrIIA4)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Oct. 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Opto-GPR149

   Plasmid

   #106049

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR149 (GPR149 Human, Bovine)
   

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available Since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. opto-E-cad GFP

   Plasmid

   #203327

   Purpose

   Expresses E-cadherin with a LOV2 insert after T133 for blue light inhibition in mammalian cells

   Depositor

   Seraphine Wegner

   Article

   Nzigou Mombo et al Nat Commun. 2023 Oct 9;14(1):6292. doi:

   Insert

   Photoswitchable - E-cadherin with GFP tag (CDH1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Addition of Aslov2 domain at T133 of human E-cadh…

   Available Since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. EphA2-YFP

   Plasmid

   #108852

   Purpose

   Encodes for human EphA2 fluorescently labeled with eYFP on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

   Depositor

   Kalina Hristova

   Article

   Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

   Insert

   EPHA2 (EPHA2 Human)
   

   Tags

   Labeled with eYFP on the C-Terminus via a 15 amin…

   Expression

   Mammalian

   Available Since

   April 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. CMV-mito-GEM-GECO1

   Plasmid

   #32461

   Purpose

   Mammalian expression of mitochondria-targeting blue-green emission ratiometric genetically encoded Ca2+ indicator for optical imaging

   Depositor

   Robert Campbell

   Article

   Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

   Insert

   GEM-GECO
   

   Tags

   a duplex of the mitochondrial targeting signal of…

   Expression

   Mammalian

   Mutation

   GCaMP3 L60P/K69E/N77Y/D86G/N98I/K119I/L173Q/T223S…

   Promoter

   CMV

   Available Since

   Dec. 5, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. NanoLuc-PM

    Plasmid

    #164784

    Purpose

    Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Human, Oplophorus gracilirostris)
    

    Tags

    NanoLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. Tau (wt) -VC

    Plasmid

    #87369

    Purpose

    expresses hTau fused to the C-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Tags

    c-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. CoV2-Spike-EF1a-D614G-N501Y

    Plasmid

    #177939

    Purpose

    Transient mammalian expression of codon optimized SARS-CoV-2 Spike proteins (D614G, N501Y). This version gives lower expression of Spike and is more convenient for generating VLPs.

    Depositor

    Jennifer Doudna

    Article

    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11

    Insert

    SARS-CoV-2 Spike protein (D614G, N501Y) (S )
    

    Expression

    Mammalian

    Mutation

    D614G, N501Y

    Available Since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. VN-Tau (wt)

    Plasmid

    #87368

    Purpose

    expresses hTau fused to the N-terminal part of Venus

    Depositor

    Tiago Outeiro

    Article

    Blum et al Hum Mol Genet. 2015 Jan 1;24(1):76-85. d

    Insert

    MAPT (MAPT Human)
    

    Tags

    N-terminal part of Venus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. GLuc-PM

    Plasmid

    #164783

    Purpose

    Gaussia luciferase (GLucM23) targeted to the extracellular surface of the cell membrane for bioluminescence resonance energy transfer (BRET)-based measurement of ligand binding to unmodified receptors

    Depositor

    Laszlo Hunyady

    Article

    Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

    Insert

    Gaussia luciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta (PDGFRB Gaussia princeps, Human)
    

    Tags

    GLuc

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMarsL274G

    Plasmid

    #63177

    Purpose

    This plasmid encodes the mouse Mars gene with the L274G mutation under a CMV promoter.

    Depositor

    David Tirrell

    Article

    Mahdavi et al J Am Chem Soc. 2016 Apr 6;138(13):4278-8

    Insert

    MarsL274G (Mars1 Mouse)
    

    Expression

    Mammalian

    Mutation

    L274G

    Promoter

    CMV

    Available Since

    April 10, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. hCGNL1-Myc-HA

    Plasmid

    #236515

    Purpose

    For expression of hParacingulinL1 in mammalian cells

    Depositor

    Sandra Citi

    Article

    Vasileva et al J Biol Chem. 2022 Apr;298(4):101797. doi

    Insert

    CGNL1 (CGNL1 Human)
    

    Tags

    Myc-HA

    Expression

    Mammalian

    Mutation

    WT

    Available Since

    April 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. EphA2-MT

    Plasmid

    #108851

    Purpose

    Encodes for human EphA2 fluorescently labeled with mTurquoise on the C-Terminus via a 15 amino acid (GGS)5 flexible linker

    Depositor

    Kalina Hristova

    Article

    Singh et al J Biol Chem. 2015 Nov 6;290(45):27271-9.

    Insert

    EPHA2 (EPHA2 Human)
    

    Tags

    Labeled with mTurquoise on the C-Terminus via a 1…

    Expression

    Mammalian

    Available Since

    April 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pStreptagII 3C SMO

    Plasmid

    #234995

    Purpose

    Expression of a Strep-tag II labeled on its N-terminus receptor, with the Smoothened (Smo) receptor

    Depositor

    Monica Gonzalez-Magaldi, Daniel Leahy

    Article

    Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

    Insert

    SMO (SMO Human)
    

    Tags

    Streptag-II, HRV 3C site

    Expression

    Mammalian

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Opto-GPR39

    Plasmid

    #106021

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR39; Opto-GPR39; B11)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR39 (GPR39 Human, Bovine)
    

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available Since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. HA-MDFIC

    Plasmid

    #207835

    Purpose

    Tagged MDFIC for western blotting and imaging

    Depositor

    Charles Cox

    Article

    Zhou et al Science. 2023 Aug 18;381(6659):799-804.

    Insert

    MDFIC (MDFIC Human)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge