167,210 results

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pCW57.1_AOX-FLAG

Plasmid

#177984

Purpose

All-in-one dox-inducible expression of Alternative Oxidase

Depositor

David Sabatini, Jessica Spinelli

Article

Spinelli et al Science. 2021 Dec 3;374(6572):1227-1237.

Insert

AOX

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Codon optimized cDNA for expression in human cells

Promoter

TRE

Available Since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCW57-RFP-P2A-MCS (Neo)

Plasmid

#89182

Purpose

All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo RFP using the P2A self-cleaving peptide.

Depositor

Adam Karpf

Article

Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

Type

Empty backbone

Use

Lentiviral; Doxycycline inducible; p2a self cleav…

Expression

Mammalian

Promoter

TRE

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pJ4M_hnRNPA2_FL_WT

Plasmid

#139109

Purpose

Express FL hnRNPA2 with a C-terminal MBP tag

Depositor

Nicolas Fawzi

Article

Ryan et al Nucleic Acids Res. 2020 Sep 1. pii: 5900

Insert

hnRNPA2 (HNRNPA2B1 Human)

Expression

Bacterial

Mutation

none

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-Hsp-eGFP-PGK-mCherry

Plasmid

#191862

Purpose

Heat inducible eGFP reporter with constitutive mCherry marker

Depositor

Yingxiao Wang

Article

Wu et al Nat Biomed Eng. 2021 Nov;5(11):1336-1347

Inserts

eGFP
mCherry

Use

Lentiviral

Expression

Mammalian

Promoter

HSPA7 promoter and PGK promoter

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLX303-ABI1-dCas9

Plasmid

#154474

Purpose

Expresses ABI1 fused to the N terminus of dCas9

Depositor

Mikko Taipale

Article

Alerasool et al Nat Methods. 2020 Nov;17(11):1093-1096.

Insert

ABI1 (ABI1 Mustard Weed)

Use

Lentiviral

Expression

Mammalian

Mutation

Includes ABI1 aa 126-423, D143A

Promoter

CMV

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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TFEB promoter-luciferase reporter

Plasmid

#66801

Purpose

Reporter for activity of TFEB gene promoter

Depositor

Albert La Spada

Article

Tsunemi et al Sci Transl Med. 2012 Jul 11;4(142):142ra

Insert

TFEB promoter (Tfeb Mouse)

Use

Luciferase

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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CMV-hRad51

Plasmid

#125570

Purpose

Expresses the Rad51 protein in mammalian cells under CMV promoter

Depositor

David Liu

Article

Rees et al Nat Commun. 2019 May 17;10(1):2212. doi:

Insert

Rad51 (RAD51 Human)

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Article

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available Since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pN251

Plasmid

#188471

Purpose

Lentiviral construct encoding doxycycline-inducible enCas12a-HF (via TetON3G)

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

enCas12a-HF (gift from Dr. Keith Joung)

Use

Lentiviral

Expression

Bacterial and Mammalian

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pTriEx-mCherry-LOV2

Plasmid

#81041

Purpose

Mammalian or insect expression for wt LOV2

Depositor

Klaus Hahn

Article

Wang et al Nat Methods. 2016 Jul 18. doi: 10.1038/n

Insert

LOV2

Tags

mCherry

Expression

Bacterial, Insect, and Mamm…

Promoter

CMV, p10

Available Since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only

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V5-LOV-Turbo-ERM

Plasmid

#199664

Purpose

expresses LOV-Turbo on the mammalian ER membrane, lentiviral vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo

Use

Lentiviral

Tags

SEC61B and V5

Expression

Mammalian

Promoter

TRE3G

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1

Plasmid

#79873

Purpose

Bacillus subtilis dCas9 expression vector; integrates into lacA/ganA

Depositor

Carol Gross, Jason Peters, Stanley Qi

Article

Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

Insert

dCas9

Use

CRISPR

Expression

Bacterial

Mutation

D10A, H840A

Promoter

xylA

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCMV4 p50

Plasmid

#21965

Depositor

Warner Greene

Article

Ballard et al Proc Natl Acad Sci U S A. 1992 Mar 1. 89

Insert

NF-kB p50 subunit (NFKB1 Human)

Expression

Mammalian

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pLV-CAG-FLEX-BFP2-(mCherry)’

Plasmid

#115234

Purpose

Lentiviral transfer plasmid for expression of mTagBFP2 before Cre recombination and mCherry after it

Depositor

Ian Wickersham

Article

Jin et al Proc Natl Acad Sci U S A. 2023 Feb 14;12

Insert

mTagBFP2 and mCherry

Expression

Mammalian

Available Since

Aug. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mCh-Rab5

Plasmid

#49201

Purpose

expression of Rab5 fused to mCherry

Depositor

Gia Voeltz

Article

Friedman et al J Cell Biol. 2010 Aug 9;190(3):363-75. d

Insert

Rab5 (RAB5B Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-ATG5

Plasmid

#99573

Purpose

Expresses Cas9 with sgRNA targeting Exon 7 of ATG5

Depositor

Edward Campbell

Article

Imam et al J Virol. 2016 Jan 13. pii: JVI.03033-15.

Insert

gRNA targeting ATG5 (ATG5 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Promoter

35S

Available Since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pYPQ132B2.0

Plasmid

#99888

Purpose

Golden Gate entry vector to express the 2nd gRNA with gRNA2.0 scaffold (with four MS2 binding sites) under AtU3 promoter

Depositor

Yiping Qi

Article

Lowder et al Mol Plant. 2017 Nov 29. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

AtU3

Available Since

March 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TFEBS122D-MYC (#777)

Plasmid

#99956

Purpose

mammalian expression

Depositor

James Brugarolas

Article

Vega-Rubin-de-Celis et al Autophagy. 2017 Mar 4;13(3):464-472. doi

Insert

TFEB (TFEB Human)

Tags

6xHis and Myc

Expression

Mammalian

Mutation

S122D

Available Since

Nov. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1_4xUb

Plasmid

#199779

Purpose

Used for the expression of 4X human ubiquitin in tandem (SMC interal no. 538)

Depositor

Sascha Martens

Article

Wurzer et al Elife. 2015 Sep 28;4:e08941. doi: 10.755

Insert

4X Ubiquitin in Tandem

Tags

GST

Expression

Bacterial

Available Since

May 12, 2023

Availability

Academic Institutions and Nonprofits only

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