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pMOS028E: Entry vector for: Mitycam calcium sensor (mitochondrial)

Plasmid

#163065

Purpose

Entry vector for: Mitycam calcium sensor (mitochondrial)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

Mitycam

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-TUBB

Plasmid

#66942

Purpose

CRISPaint target selector TUBB

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA TUBB

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pfMDH_WT

Plasmid

#67721

Purpose

Expresses WT mannitol 2-dehydrogenase from Pseudomonas fluorescens, codon optimized for expression in E. coli

Depositor

Justin Siegel

Article

Lucas et al Protein Sci. 2015 Jun;24(6):936-45. doi:

Insert

Mannitol 2-Dehydrogenase from Pseudomonas fluorescens

Tags

6x-His tag

Expression

Bacterial

Promoter

T7

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
HI.fate.SFFV-E

Plasmid

#191555

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.E

Plasmid

#191553

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pWS5396-CRISPRai-KanR

Plasmid

#182835

Purpose

Saccharomyces cerevisiae inducible CRISPRai vector - KanR marker

Depositor

Rodrigo Ledesma-Amaro

Article

Shaw et al Nat Commun. 2022 Aug 25;13(1):4984. doi:

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnd3

Plasmid

#159912

Purpose

Mutagenesis of Kcnd3

Depositor

Larry Zweifel

Insert

Kcnd3 (Kcnd3 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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HI.fate.UB

Plasmid

#191556

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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HI.fate.SFFV

Plasmid

#191554

Purpose

Monitor HIV-1 fate

Depositor

Alon Herschhorn, Joseph Sodroski

Article

Ratnapriya et al Cell Rep. 2021 Aug 31;36(9):109622. doi:

Insert

HIV-1 incomplete genome

Use

Lentiviral

Mutation

env-deleted + vpr mutation(s)

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-3-SH3BP1

Plasmid

#136647

Purpose

Expression of SHBP1-GST fusion protein in E. coli

Depositor

Maria Balda, Karl Matter

Article

Elbediwy et al J Cell Biol. 2012 Aug 20;198(4):677-93.

Insert

SH3BP1 (SH3BP1 Human)

Expression

Bacterial

Available Since

Feb. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP44-2A(sfGFP)

Plasmid

#126988

Purpose

Level 2 vector. sfGFP reporter. Origin pRO1600/ColE1 (E.coli - Pseudomonas shuttle)

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pMJ332: pU6-GtFz1_omegaRNA-entry

Plasmid

#205265

Purpose

Human expression vector for GtFz1 omegaRNA (BbsI guide entry site)

Depositor

Feng Zhang

Article

Saito et al Nature. 2023 Jun 28. doi: 10.1038/s41586

Insert

GtFz1 omegaRNA

Expression

Mammalian

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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TOPO mTagBFP2

Plasmid

#68413

Purpose

TOPO construct expressing mTagBFP2 for generation of GMAP-compatible gene

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

mTagBFP2

Use

Gmap

Tags

6xHis

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pSTK120-mCMV-TK

Plasmid

#22054

Depositor

Maria Castro

Article

King et al J Virol. 2008 May . 82(9):4680-4.

Insert

mCMV-TK-pA expression cassette

Use

Adenoviral

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pOGG024

Plasmid

#113991

Purpose

Level 1 golden gate cloning vector for medium copy number free-living expression

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Type

Empty backbone

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-His-APEX2

Plasmid

#201471

Purpose

Recombinant soluble APEX2 for SLAPSHOT labeling

Depositor

Yuh-Nung Jan

Article

Tuomivaara et al Commun Biol. 2024 Aug 29;7(1):1060. doi:

Insert

APEX2

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pRRL-VinculinCS

Plasmid

#200309

Purpose

Lentivirus vector expressing vinculin with FRET conformational sensor

Depositor

Brenton Hoffman

Article

Tao et al Dev Cell. 2023 Mar 10:S1534-5807(23)0007

Insert

VinculinCS

Use

Lentiviral

Promoter

T7

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pOZC-PALB2

Plasmid

#133552

Purpose

PALB2 coding sequence in pOZ-FH-C C-terminal Flag, His tagging retroviral vector.

Depositor

David Livingston

Article

Xia et al Mol Cell. 2006 Jun 23. 22(6):719-29.

Insert

PALB2 (PALB2 Human)

Use

Retroviral

Available Since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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PBS Actb-2A-TIR1-GFP

Plasmid

#113832

Purpose

targeting osTIR1-GFP into mouse Actb locus for auxin inducible degradation

Depositor

Janet Rossant

Article

Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

Insert

osTIR1-GFP (NEWENTRY Synthetic)

Use

Synthetic Biology

Available Since

Aug. 30, 2018

Availability

Academic Institutions and Nonprofits only

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lenti-fullef1a-mutFAKSPARK2

Plasmid

#195266

Purpose

express Tyr to Phe mutant FAKSPARK2 in a lenti vector

Depositor

Xiaokun Shu

Article

Li et al Nat Chem Biol. 2023 Jun 22. doi: 10.1038

Insert

Tyr to Phe mutant FAKSPARK2 sensor

Use

Lentiviral

Expression

Mammalian

Promoter

ef1a

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pET303-colE1TR

Plasmid

#155181

Purpose

Expresses colicin E1 residues 1-364

Depositor

Joanna Slusky

Article

Budiardjo et al Elife. 2022 Feb 24;11. pii: 73297. doi:

Insert

colicin E1 residues 1-364

Tags

C-terminal 6x Histidine tag

Expression

Bacterial

Mutation

truncation of colicin E1 including 1-364

Promoter

T7

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet-I_IRES_sYFP2_PH_N1

Plasmid

#110623

Purpose

It expresses separate mScarletI pHdomain and SYFP2 pHdomain in a 3:1 ratio.

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH mScarlet-I- IRES- sYFP2 PH

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pJUMP18-Histag_C

Plasmid

#127035

Purpose

Basic Part C- C-terminus; polyhistidine tag

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Insert

Part His6_C

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 1, 2019

Availability

Academic Institutions and Nonprofits only

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pBS-IT6

Plasmid

#66870

Purpose

Inntag 6: a small self-structured epitope aimed at innocuous protein tagging

Depositor

Marti Aldea

Article

Georgieva et al Nat Methods. 2015 Aug 31. doi: 10.1038/n

Insert

Inntag 6

Available Since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pDisplay-Gncp-iGluSnFR

Plasmid

#107337

Purpose

Noncircularly permutated (ncp) variant of iGluSnFR. Membrane-displayed.

Depositor

Robert Campbell

Article

Wu et al ACS Chem Biol. 2018 Jan 22. doi: 10.1021

Insert

GltI based and noncircularly permutated glutamate biosensor

Expression

Mammalian

Promoter

CMV

Available Since

May 3, 2018

Availability

Academic Institutions and Nonprofits only

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pG-HIV-LRT

Plasmid

#104592

Purpose

Standard for qPCR

Depositor

Kristine Yoder

Article

Yoder et al J Virol Methods. 2008 Nov;153(2):253-6.

Insert

HIV-1 late reverse transcripts

Use

Cloning vector

Available Since

Jan. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CTLA4-mgSrtA

Plasmid

#125794

Purpose

for co-expression of CTLA4 and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of CD80/86 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

CTLA4-mgSrtA

Expression

Mammalian

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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LV anti-FITC-LaG16-Y186F-T2A-BFP

Plasmid

#194931

Purpose

LV construct encoding Y186F mutant sNRR-based SynNotch receptor

Depositor

John Ngo

Article

Sloas et al Nat Biotechnol. 2023 Jan 16. doi: 10.103

Insert

antiFITC-LaG16-sNRR(Y186F)-SynNotch-Gal4-VP64-T2A-BFP

Use

Lentiviral and Synthetic Biology

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-ZHER-mgSrtA

Plasmid

#125793

Purpose

for co-expression of ZHER (a HER2 binding affibody) and mgSrtA on cell membrane. For proof-of-concept monitoring and labeling of HER2 positive cells.

Depositor

P. Chen

Article

Ge et al J Am Chem Soc. 2019 Feb 6;141(5):1833-18

Insert

ZHER-mgSrtA

Expression

Mammalian

Available Since

May 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA HA FAK Y925F

Plasmid

#50509

Depositor

Kenneth Yamada

Article

Tamura et al J Biol Chem. 1999 Jul 16;274(29):20693-7

Insert

protein tyrosine kinase, mutated

Tags

HA

Expression

Mammalian

Mutation

Y925F

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only