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NAX_CAGGS_CAS9_Puro

Plasmid

#167863

Purpose

PiggyBac compatible plasmid expressing spCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1-Cdc45

Plasmid

#211426

Purpose

mammalian expression of human Cdc45 C-terminally fused to EGFP

Depositor

Helmut Pospiech

Article

Kohler et al Cell Cycle. 2016;15(7):974-85. doi: 10.1

Insert

Cdc45 (CDC45 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-CD4-sTb(C)-M13-GFP

Plasmid

#219784

Purpose

sTb(C) half of CaST for neuron expression

Depositor

Christina Kim

Article

Zhang et al Nat Methods. 2024 Sep;21(9):1725-1735. d

Insert

CD4-sTb(C)-M13-GFP

Use

AAV

Tags

HA

Expression

Mammalian

Promoter

Synapsin

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-hsyn-GRAB_HA1mut

Plasmid

#190475

Purpose

Express the histamine-insensitive control sensor GRAB_HA1mut in neurons

Depositor

Yulong Li

Article

Dong et al Neuron. 2023 Mar 7:S0896-6273(23)00128-9

Insert

Histamine-insensitive sensor GRAB_HA1mut (control sensor)

Use

AAV

Promoter

hSyn

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pMOS016E: Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163075

Purpose

Entry vector for: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Gateway entry vector

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Hygro

Plasmid

#167907

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_TEV_mVenus_FG

Plasmid

#240673

Purpose

Expression plasmid for mCherry-TEV-mVenus-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_TEV_2xStrepII_FG

Plasmid

#240675

Purpose

Expression plasmid for TEV-2xStrepII-tagged proteins with FAST-Green transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

TEV-2xStrepII

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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pG20_mCherry_TEV_mVenus_Hyg

Plasmid

#240653

Purpose

Expression plasmid for mCherry-TEV-mVenus-tagged proteins with Hygromycin transgenic seed selection

Depositor

Hans-Henning Kunz

Article

Schwartz et al Plant J. 2025 Nov;124(3):e70544. doi: 10

Type

Empty backbone

Tags

mCherry-TEV-mVenus

Expression

Plant

Available Since

Nov. 12, 2025

Availability

Academic Institutions and Nonprofits only

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ClCry4 PHR

Plasmid

#160552

Purpose

Expression of protein for various experiments including finding interaction partners

Depositor

Joseph Takahashi

Article

Zoltowski et al Proc Natl Acad Sci U S A. 2019 Sep 24;11

Insert

Clcry4 HR

Tags

Hexa his

Expression

Insect

Available Since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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CRISPR_HLA_class_I_2

Plasmid

#164987

Purpose

Expression of gRNA targeting multiple HLA-A, HLA-B, and HLA-C genes

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

gRNA against HLA class I

Use

CRISPR

Expression

Mammalian

Available Since

March 19, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-SAC1deltaTMD

Plasmid

#108132

Purpose

Recruitable cytosolic SAC1

Depositor

Gerry Hammond

Article

Zewe et al Elife. 2018 Feb 20;7. pii: 35588. doi: 1

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:SACM1L(1-521) (SACM1L Human)

Expression

Mammalian

Mutation

SACM1L(1-521)

Promoter

CMV

Available Since

April 6, 2018

Availability

Academic Institutions and Nonprofits only

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mEmerald-MCM2-N-22

Plasmid

#54164

Purpose

Localization: Nucleus, Excitation: 487, Emission: 509

Depositor

Michael Davidson

Article

Kuipers et al J Cell Biol. 2011 Jan 10;192(1):29-41. d

Insert

MCM2

Tags

mEmerald

Expression

Mammalian

Promoter

CMV

Available Since

July 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pCI5-CasRx-NES-HA (pKW387)

Plasmid

#229048

Purpose

Mammalian expression of CasRx-NES-HA

Depositor

S Chul Kwon

Article

Cheng et al EMBO Rep. 2025 Feb 26. doi: 10.1038/s443

Insert

CasRx (RfxCas13d)

Use

CRISPR

Tags

NES-HA

Expression

Mammalian

Available Since

Feb. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pAAVS1-Puro-PECAM1-Neo

Plasmid

#201054

Purpose

Endothelial promoter driven neomycin selection cassette

Depositor

Paul Burridge

Article

Pinheiro et al Sci Rep. 2024 Mar 26;14(1):7123. doi: 10

Insert

Adeno-associated virus integration site 1 (AAVS1 Human)

Use

AAV

Tags

Neomycin resistance

Promoter

PECAM1

Available Since

July 9, 2025

Availability

Academic Institutions and Nonprofits only

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pBXNH3CA_MBP

Plasmid

#132700

Purpose

expresses Avi-tagged MBP in E.coli, to use as positive control in ELISA

Depositor

Markus Seeger

Article

Zimmermann et al Nat Protoc. 2020 Apr 8. pii: 10.1038/s41

Insert

Maltose binding Protein

Tags

Avi and His

Expression

Bacterial

Promoter

pBAD

Available Since

Oct. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pTalpha1-iCre

Plasmid

#133924

Purpose

Neuron-specific expression of iCre

Depositor

Frank Bradke, Sebastián Dupraz

Article

Dupraz et al Curr Biol. 2019 Nov 18;29(22):3874-3886.

Insert

iCre

Expression

Mammalian

Mutation

Codon optimized

Promoter

neuron-specific tubulin-alpha-1 promoter

Available Since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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dCas12j3 KRAB

Plasmid

#188507

Purpose

Expresses FLAG tagged dCas12j3 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas12j3

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

D413A

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pFLAG/HA-DGCR8

Plasmid

#10921

Depositor

Thomas Tuschl

Article

Landthaler et al Curr Biol. 2004 Dec 14. 14(23):2162-7.

Insert

DGCR8 (DGCR8 Human)

Tags

FLAG and HA

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pCIP-caTfeb

Plasmid

#79013

Purpose

For mammalian expression of a shortened, constitutively active version of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (TFEB Human)

Expression

Mammalian

Mutation

Removed first ~400 bp from 5' end (N-terminu…

Promoter

pCAGGs

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only
pSUPER-retro-puro-shNup93-XhoI

Plasmid

#87330

Purpose

To express shRNA against human Nup93

Depositor

Lei Lu

Article

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available Since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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Ki67∆FHA-mNeonGreen-IRESpuro2

Plasmid

#183739

Purpose

Expresses Ki67∆FHA-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67_lacking FHA (MKI67 Human)

Tags

mNeonGreen

Expression

Bacterial and Mammalian

Mutation

MKI67(lacking FHA)

Promoter

CMV

Available Since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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NpY-mNeon

Plasmid

#186618

Purpose

Encodes Neuropeptide Y fused to mNeonGreen

Depositor

Gia Voeltz

Article

Zamponi et al Dev Cell. 2022 Jun 6;57(11):1369-1382.e6

Insert

Neuropeptide Y (NPY Human)

Tags

mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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Cyto-ABKAR

Plasmid

#61510

Purpose

Expression of AMPK biosensor

Depositor

Takanari Inoue, Jin Zhang

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Insert

AMPK Biosensor

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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M8

Plasmid

#180253

Purpose

Vaccine vector encoding 8 neonatigens specific for the MC38 colon cancer mouse model

Depositor

Fabio Palombo

Article

Salvatori et al NPJ Vaccines. 2022 Feb 2;7(1):15. doi: 1

Insert

Synthetic gene encoding for 8 neonatigens specific for MC38 mouse cancer cells

Use

Vaccine vector

Expression

Mammalian

Mutation

the polyepitope vaccine encodes for mutated seque…

Promoter

CMV

Available Since

Aug. 6, 2025

Availability

Academic Institutions and Nonprofits only

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OPN(-1206)-luc

Plasmid

#31106

Depositor

Gerhart Ryffel

Article

Senkel et al Biochim Biophys Acta. 2005 Dec 20. 1731(

Insert

osteopontin promoter (SPP1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

promoter region -1206 to +87

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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FRT LAMP3 mCRISPRed_mTagBFP2 pcDNA5

Plasmid

#159455

Purpose

Encodes a lysosome tagret fusion protein of the pH resistant proteins mCRISPRed and mTagBFP2

Depositor

Oliver Griesbeck

Article

Erdogan et al Cell Chem Biol. 2020 May 21;27(5):610-62

Insert

mCRISPRed

Tags

mTagBFP2

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pBa.Kif1a 1-396.GFP

Plasmid

#45058

Depositor

Gary Banker, Marvin Bentley

Article

Jacobson et al Neuron. 2006 Mar 16;49(6):797-804.

Insert

Kif 1a (Kif1a Rat)

Tags

GFP

Expression

Mammalian

Mutation

motor domain aa 1-396

Promoter

Chicken Beta Actin

Available Since

Oct. 7, 2013

Availability

Academic Institutions and Nonprofits only
pBWB419

Plasmid

#140637

Purpose

RSF1010-Cas9-pcaHG gRNA. Vector with constitutive Cas9 with gRNA targeting pcaHG locus.

Depositor

Keith Tyo

Article

Biggs et al Nucleic Acids Res. 2020 Apr 4. pii: 5815

Insert

Cas9

Expression

Bacterial

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
AsCas12a

Plasmid

#188492

Purpose

Expresses FLAG tagged AsCas12a

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

Cas12a

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBabepuroBAF180

Plasmid

#41078

Depositor

Ramon Parsons

Article

Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

Insert

BAF180 (PBRM1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

3 silent mutations: 3595 a to g; 4981 a to t; 587…

Available Since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Plasmid

#87644

Purpose

For creating drosophila transgenics expressing UAS->Bxb1.STOP>SNAPf>CLIPf>Halo2

Depositor

Gregory Jefferis

Article

Sutcliffe et al Genetics. 2017 Apr;205(4):1399-1408. doi

Insert

myr::SNAPf>myr::CLIPf>myr::Halo2

Expression

Insect

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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pBR322-Pdp1_NTD-Cre-HiBiT

Plasmid

#198318

Purpose

Loads HiBiT-tagged Cre into PVCs using Pdp1 packaging domain; also contains Pnf

Depositor

Feng Zhang

Article

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

Pdp1_NTD-Cre-HiBiT

Expression

Bacterial

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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Calreticulin-SuperNova-KDEL

Plasmid

#131857

Purpose

Expresses SuperNova fused to ER protein calreticulin and KDEL ER-retention signal

Depositor

Geert van den Bogaart

Article

Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

Insert

SuperNova

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pTetR-LasR-pLuxR-GFP

Plasmid

#171159

Purpose

Sensing Device with Reporter protein

Depositor

Matthew Chang

Article

Saeidi et al Mol Syst Biol. 2011 Aug 16;7:521. doi: 1

Inserts

lasR
gfp

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux and pTet

Available Since

July 15, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDA007

Plasmid

#131380

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Tags

ORF2-3xFlag

Expression

Mammalian

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-jGCaMP7b-XC

Plasmid

#182221

Purpose

Improved genetic encoded calcium sensor based on GCaMP7b.

Depositor

Xiaodong Liu

Article

Geng et al Elife. 2022 Oct 5;11:e76691. doi: 10.755

Insert

jGCaMP7b-XC

Use

AAV

Expression

Mammalian

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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GI-Gal4 DBD

Plasmid

#42500

Purpose

GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a light-inducible transcription factor

Depositor

Ricardo Dolmetsch

Article

Yazawa et al Nat Biotechnol. 2009 Oct;27(10):941-5. d

Insert

GIGANTEA fused with Gal4 DBD

Tags

Gal4 DNA binding domain (DBD)

Expression

Mammalian

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pSEVA221pmCDA1-T7RNAP

Plasmid

#167978

Purpose

Expresses fusion protein pmCDA1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

pmCDA1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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We narrowed to 31,857 results for: ica