We narrowed to 18,395 results for: Tec
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pcDNA3.1(+)-sapphireAKAR(T/A)
Plasmid#118465PurposeNegative-control mutant for sapphireAKAR biosensor.DepositorInsertsapphireAKAR(T/A)
UseTags6xHis, T7 tag (gene 10 leader), and Xpress (TM) t…ExpressionMammalianMutationContains Thr-to-Ala mutation in substrate sequenc…PromoterCMVAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMetYCgate
Plasmid#105074PurposeYeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloningDepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterPBenAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
RS18960_pMQ30_KO_Kan
Plasmid#185393PurposeNon-replicating vector used to create markerless deletion of RR42_RS18960 in C. basilensis 4G11DepositorInsertsRR42_RS18960 upstream flanking homology region
RR42_RS18960 downstream flanking homology region
UseTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pCH68 (dAsCas12a-3xKRAB piggyBac)
Plasmid#217334PurposepiggyBac expression of dAsCas12a-3xKRABDepositorInsertdAsCas12a
UseCRISPRTagsnucleoNLS-3xKRAB-3xHA-P2A-TagBFP2ExpressionMammalianMutationE993APromoterCAGAvailable sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCfB3034(X-3 MarkerFree)
Plasmid#73272PurposeEasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site X-3 (Chr X: 223616..224744)DepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPMVAugw
Plasmid#98300PurposeEnhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of CUP1 promoter.DepositorInsertTEFM1
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pME-mKate-P2A-APEX2-NS
Plasmid#67670PurposeGateway entry clone containing mKate2-P2A-APEX2 with no stop codonDepositorInsertmKate2-P2A-APEX2-NS
UseGateway multisite middle entry cloneTagsExpressionMutationPromoterAvailable sinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTS2337_Tier3(SB)-TagBFP2_Puro
Plasmid#169635PurposeTier-3 vector for stable integration of up to three cassettes via sleeping beauty transposase including a TagBFP marker and PuroR resistance gene (5'ITR-A1-pA::A2-pA::PRPBSA-mTagBFP2-p2A-PuroR-pA-3'ITR)DepositorInsertPRPBSA-driven mTagBFP2 and PuroR expression
UseTagsExpressionMammalianMutationPromoterPRPBSAAvailable sinceJuly 13, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisTagsExpressionMutationPromoterOR2-OR1-PrAvailable sinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSI-535
Plasmid#131130PurposegRNA expression vector for C-to-T base editing reporterDepositorInsertC-to-T reporter activation gRNA
UseCRISPRTagsExpressionMammalianMutationPromoterHuman U6 promoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2-GFP-TAG
Plasmid#158131PurposeSingle fluorescent protein reporter (GFP) for ncAA incorporation (TAG construct)DepositorInsertsfGFP
UseTagsExpressionYeastMutationIntroduced a stop codon in place of the tyrosine …PromoterGAL1-10Available sinceJan. 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
CP1028-ABE8e-dimer
Plasmid#138498PurposeA-to-G base editorDepositorInsertecTadA(WT)-ecTadA(8e)-nSpCas9 (CP1028)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
PML-ER-pBabe
Plasmid#73556Purposevector encoding a fusion protein between PML-IV and ER (estrogen receptor)DepositorInsertPML-IV (PML Human)
UseRetroviralTagsERExpressionMammalianMutationPromoterSV40Available sinceAug. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pLV-EF1A-LivePAR(R163A)-Hygro
Plasmid#187609PurposeEGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.DepositorInsertLivePAR(R163A)
UseLentiviralTagsEGFPExpressionMammalianMutationR163A mutation in the WWE DomainPromoterEF1AAvailable sinceSept. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOCC267
Plasmid#118887Purposeshuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with N-terminal HIS6-MBP tag, cleavable with 3C, and N-terminal HALO tagDepositorInsertNcoI-HIS6-MBP-3C-HALO-NotI-ccdB-AscI-stop-HindIII cassette
UseTagsHIS6-MBP, cleavable with 3C protease; HALO tagExpressionInsectMutationPromoterpolHAvailable sinceJan. 2, 2019AvailabilityAcademic Institutions and Nonprofits only
