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pFUSEss-CHIg-mG1_CH3-3

Plasmid

#105853

Purpose

Expression plasmid coding for hybrid heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18. The antibody comprises IgG3-derived CH3.

Depositor

Joanna Bereta

Article

Klaus et al Front Immunol. 2018 May 23;9:1096. doi:

Insert

IgG1 heavy chain, IgG3 heavy chain (Ighg1, Ighg3 Mouse)

Expression

Mammalian

Mutation

hybrid heavy chain of mouse IgG1 with CH3 derived…

Promoter

hEF1-HTLV prom

Available Since

April 25, 2018

Availability

Academic Institutions and Nonprofits only

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p156 pWZL-EGFP-SLPI

Plasmid

#11069

Depositor

Ramesh Shivdasani

Article

Schulze et al Blood. 2004 Dec 15. 104(13):3949-57.

Insert

SLPI (Slpi Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Available Since

Dec. 6, 2005

Availability

Academic Institutions and Nonprofits only
pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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sgLacZs

Plasmid

#171185

Purpose

Expresses sgRNA

Depositor

Han Kyoung Choe

Article

Kim et al Sci Rep. 2021 Jan 28;11(1):2575. doi: 10

Insert

hU6-sgLacZ1-hU6-sgLacZ2

Use

AAV

Tags

mCherry

Promoter

U6, hSyn

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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CAPZB2-TwinStrepII

Plasmid

#83194

Purpose

Expression of tagged protein in mammalian cells

Depositor

Peter Barr-Gillespie

Insert

CAPZB2 (Capzb Mouse)

Tags

Twin Strep Tag II

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pEGFP-RapR-FAK-YM-Y397F

Plasmid

#25930

Depositor

Klaus Hahn

Article

Karginov et al Nat Biotechnol. 2010 Jul;28(7):743-7. do

Insert

RapR-FAK (Ptk2 Human, Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

This pEGFP-RapR-FAK-YM-Y397F plasmid contains the…

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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EF1a_DIO_R106W-HaloTag

Plasmid

#164063

Purpose

Double-Floxed Inverted Open reading frame for mouseMeCP2(alpha) carrying R106W mutation

Depositor

Nathaniel Heintz

Article

Piccolo et al Elife. 2019 Dec 23;8. pii: 51449. doi: 1

Insert

MeCP2 R106W (Mecp2 Mouse)

Use

AAV

Tags

HaloTag

Expression

Mammalian

Promoter

EF1A

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pWPXL-p150

Plasmid

#160992

Purpose

Express fulllength of mouse p150 protein

Depositor

Zhiguo Zhang

Article

Cheng et al Nucleic Acids Res. 2019 Dec 2;47(21):111

Insert

CHAF1A (Chaf1a Mouse)

Tags

GFP

Expression

Mammalian

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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Pvalb-2A-Dre Flp-in replacement vector

Plasmid

#61571

Purpose

Recombinase-mediated cassette exchange in ES cells to insert the Dre recombinase gene at the mouse Pvalb gene stop codon

Depositor

Hongkui Zeng

Article

Madisen et al Neuron. 2015 Mar 4;85(5):942-58. doi: 10

Insert

Pvalb-2A-Dre (Pvalb D6 Bacteriophage, Mouse)

Use

Recombinase-mediated cassette exchange using flp …

Available Since

Feb. 9, 2015

Availability

Academic Institutions and Nonprofits only

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p214_LTJ_1kbCD90.1Template

Plasmid

#82667

Purpose

1kb HDR template for murine CD90.1

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

1kb genomic DNA sequence from murine CD90.1

Use

Hdr template for gene editing

Available Since

Feb. 26, 2018

Availability

Academic Institutions and Nonprofits only

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mApple-LC-Myosin-C-10

Plasmid

#54919

Purpose

Localization: Light Chain Myosin, Excitation: 568, Emission: 592

Depositor

Michael Davidson

Insert

LC-Myosin

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 7, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac mph1 flag

Plasmid

#1985

Depositor

Robert Kingston

Article

Lavigne et al Mol Cell 2004 Feb 13;13(3):415-25.

Insert

mPh1 (Phc1 Mouse)

Tags

flag

Expression

Insect

Available Since

March 23, 2006

Availability

Academic Institutions and Nonprofits only
sg1+sg2+sg3

Plasmid

#113969

Purpose

Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

Depositor

Jordan Green

Article

Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

Insert

sg1+sg2+sg3

Expression

Mammalian

Promoter

U6

Available Since

May 28, 2020

Availability

Academic Institutions and Nonprofits only

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pMRX-IP/Venus-mATG5

Plasmid

#58739

Purpose

Expresses Venus-mATG5 in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Retroviral

Tags

Venus

Available Since

Aug. 4, 2015

Availability

Academic Institutions and Nonprofits only
pSicoR-sh4EBP2

Plasmid

#81121

Purpose

expresses an shRNA targeting murine 4E-BP2

Depositor

Angelique Bordey

Article

Hartman et al Cell Rep. 2013 Oct 31;5(2):433-44. doi:

Insert

4E-BP2 shRNA (Eif4ebp2 Mouse)

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 21, 2016

Availability

Academic Institutions and Nonprofits only

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pOC3 smFP-HA-Cacna1e KI

Plasmid

#183436

Purpose

CreON FlpOFF knock-in for smFP-HA-CaV2.3 (amino acid position: G5)

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

gRNA and smFP HA donor

Use

CRISPR

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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Anti-Tiam1 [L130/1R]

Plasmid

#177476

Purpose

Mammalian Expression Plasmid of anti-Tiam1 (Mouse). Derived from hybridoma L130/1.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-Tiam1 (Mus musculus) recombinant mouse monoclonal antibody (Tiam1 Mouse)

Expression

Mammalian

Promoter

Dual CMV

Available Since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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RV-Syn-Dio-yfp_2a_neto1

Plasmid

#98176

Purpose

Retroviral vector encoding Cre-dependent expression of EYFP-P2A-Neto1

Depositor

Oscar Marin

Insert

Neto1 (Neto1 Mouse)

Use

Retroviral

Promoter

Syn

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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FLAG-KIF5A

Plasmid

#166954

Purpose

Expresses FLAG-tagged KIF5A protein in pcDNA3.1

Depositor

Rosalind Segal

Article

Fukuda et al J Cell Biol. 2021 Jan 4;220(1). pii: 211

Insert

KIF5A (Kif5a Mouse)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1a-mRor2deltaPro

Plasmid

#22634

Depositor

Roel Nusse

Article

Mikels et al J Biol Chem. 2009 Oct 30;284(44):30167-7

Insert

Ror2 delta Pro (Ror2 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Deletion of Pro domain

Available Since

Dec. 18, 2009

Availability

Academic Institutions and Nonprofits only