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pBABE-hygro NM23 H1 WT

Plasmid

#11364

Depositor

Ronald Kahn

Publication

Zhu et al Proc Natl Acad Sci U S A. 1999 Dec 21. 9

Insert

NM23 H1 (NME1 Human)

Use

Retroviral

Expression

Mammalian

Available since

March 1, 2006

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pCX-ThPDE5VV

Plasmid

#134778

Purpose

cGMP FRET biosensor

Depositor

Xavier Nicol

Publication

Ros et al Cell Rep. 2019 Jun 25;27(13):4003-4012.e

Insert

ThPDE5VV

Tags

cpVenus-Venus and mTurquoise2

Expression

Mammalian

Promoter

CAG

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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smHA multi-frame tag

Plasmid

#128611

Purpose

a spaghetti monster with 10x HA epitopes repeat upstream of the HIV frameshift sequence which is followed by FLAG epitopes in the 0ORF are separated from one another by SunTag epitopes in the -1ORF.

Depositor

Tim Stasevich

Publication

Lyon et al Mol Cell. 2019 Jul 11;75(1):172-183.e9.

Insert

smHA MF tag

Expression

Mammalian

Available since

July 24, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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DS-TDcasN-

Plasmid

#48660

Purpose

Bacterial nuclease-null TD Cas9 expression

Depositor

George Church

Publication

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Inserts

Cas9, nuclease-null
TD tracrRNA

Use

CRISPR

Mutation

D to A, DH to AA, N to A

Promoter

proC and tracrRNA promoter

Available since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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psiCHECK2-let-7 2x

Plasmid

#20929

Depositor

Yukihide Tomari

Publication

Iwasaki et al Mol Cell. 2009 Apr 10;34(1):58-67. doi:

Insert

2x let-7 target sites

Use

Luciferase

Expression

Insect and Mammalian

Available since

June 19, 2009

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pCM5.36

Plasmid

#19394

Depositor

Geraldine Seydoux

Publication

Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

Insert

gld-1 3'utr in pDONRP2RP3 (gld-1 Nematode)

Expression

Worm

Available since

Nov. 10, 2008

Availability

Academic Institutions and Nonprofits only

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pBR322 14-3-3 Promoter

Plasmid

#16517

Depositor

Bert Vogelstein

Publication

Hermeking et al Mol Cell. 1997 Dec . 1(1):3-11.

Insert

14-3-3 promoter (SFN Human)

Expression

Bacterial

Available since

July 27, 2008

Availability

Academic Institutions and Nonprofits only
NanoLuc-PM-Venus

Plasmid

#164786

Purpose

Nanoluciferase (NanoLuc) targeted to the extracellular surface of the cell membrane and intracellularly labeled with monomeric Venus fluorescent protein

Depositor

Laszlo Hunyady

Publication

Toth et al J Biol Chem. 2021 Feb 2:100366. doi: 10.

Insert

Nanoluciferase fused with the transmembrane domain of the platelet-derived growth factor receptor beta and Venus (PDGFRB Human, Oplophorus gracilirostris)

Tags

NanoLuc and mVenus

Expression

Mammalian

Promoter

CMV

Available since

May 19, 2021

Availability

Academic Institutions and Nonprofits only

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pRSET-QNA

Plasmid

#129305

Purpose

Negative control for ATP measurement

Depositor

Hiroyuki Noji

Publication

Yaginuma et al Sci Rep. 2014 Oct 6;4:6522. doi: 10.1038

Insert

QUEEN-NA

Expression

Bacterial

Available since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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SaCas9_sgRNA_expression_in_pBluescript

Plasmid

#122090

Purpose

U6 driven SaCas9 sgRNA expression vector for cloning own guides

Depositor

Scot Wolfe

Publication

Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

Insert

SaCas9 tracr (NEWENTRY S. pyogenes, Synthetic)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

April 18, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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lentiCRISPRv2_sgCtrl#1

Plasmid

#174148

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pCAG-Cre-iBSD

Plasmid

#140284

Purpose

Expression of Cre-IRES-BSD

Depositor

Yusuke Miyanari

Publication

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

Cre

Expression

Mammalian

Available since

May 1, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMD19_STARR-seq_plants

Plasmid

#117379

Purpose

STARR-seq vector with 35s core promoter for transient expression in plant protoplasts

Depositor

Xiaoyu Zhang

Publication

Ricci et al Nat Plants. 2019 Nov 18. pii: 10.1038/s4

Type

Empty backbone

Tags

GFP derived from pMDC107

Expression

Plant

Promoter

35s core promoter

Available since

Nov. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pJUMP27-1A(sfGFP)

Plasmid

#126974

Purpose

Level 1 vector. sfGFP reporter. Origin pSC101 (low copy number)

Depositor

Chris French

Publication

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMyBADC

Plasmid

#84688

Purpose

Protein expression in mycobacteria

Depositor

Matthias Wilmanns

Publication

Beckham et al Protein Sci. 2020 Oct 2. doi: 10.1002/pr

Type

Empty backbone

Use

Bacterial expression vector

Tags

His6

Expression

Bacterial

Promoter

Arabinose

Available since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pICH41421

Plasmid

#50339

Depositor

Sylvestre Marillonnet, Nicola Patron

Publication

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, nos (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

5 Articles

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pEN_TmiR_Luc-A

Plasmid

#25760

Purpose

Entry vector withTRE promoter driving control Luciferase miR30-based shRNA.

Depositor

Iain Fraser

Publication

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

Luciferase miR-shRNA

Use

Gateway Cloning

Available since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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FLAG-ARF

Plasmid

#89123

Purpose

mammalian expression of p14-hARF with N-terminal FLAG tag

Depositor

Elizabeth Wilson

Publication

Minges et al J Biol Chem. 2015 Oct 9;290(41):25174-87

Insert

p14-hARF (CDKN2A Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCS5-3HA/p14ARF

Plasmid

#78764

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Publication

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

p14ARF (CDKN2A Human)

Tags

3xHA

Expression

Mammalian

Promoter

CMV IE94

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pcDNAI-GAL4-CREB-S133A

Plasmid

#46770

Purpose

Expression of GAL4 fusion to CREB-S133A

Depositor

Richard Maurer

Publication

Sun et al Genes Dev. 1994 Nov 1;8(21):2527-39.

Insert

GAL4(1-147)-CREB-S133A (Creb1, GAL4 Budding Yeast, Rat)

Tags

GAL4

Expression

Mammalian

Mutation

Changed CREB serine 133 to Alanine

Promoter

CMV

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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