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Showing: 8611 - 8640 of 21422 results

  2. pKB01_tagRFP

    Plasmid
    #69103
    Purpose
    Expresses tagRFP (codon adapted for Streptococcus pneumoniae); under control of Zinc inducible promoter PZn. Plasmid integrates in S. pneumoniae bgaA locus by double cross-over.
    Depositor
    Jan-Willem Veening
    Article
    Beilharz et al Appl Environ Microbiol. 2015 Aug 7. pii:
    Insert
    tagRFP
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Oct. 2, 2015
    Availability
    Academic Institutions and Nonprofits only
    69103-plasmid-map-sequence-id-125507

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  5. pKL004

    Plasmid
    #98920
    Purpose
    Constitutively expresses the calcium sensor GCaMP6f tethered to mRuby3 under the Sigma 70 promoter in E. coli.
    Depositor
    Joel Kralj
    Article
    Bruni et al Proc Natl Acad Sci U S A. 2017 Aug 29;11
    Insert
    GCaMP6f tethered to mRuby
    Use
    Tags
    His and mRuby3
    Expression
    Bacterial
    Mutation
    Promoter
    BBa_J23118 - Strong Constitutive
    Available since
    May 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    98920-plasmid-map-sequence-id-189873

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  7. ZJOM27

    Plasmid
    #133641
    Purpose
    Integration of GFP-PHO8 as an additional copy in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pCUP1-GFP-Pho8 (PHO8 Budding Yeast)
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133641-plasmid-map-sequence-id-268964

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  11. pBDK

    Plasmid
    #135190
    Purpose
    Integration and excision of kanamycin cassette for the E. coli genome via CreI, SceI, and lamba Red system. Contains SacB selection
    Depositor
    Hongqing Fang
    Article
    Wei et al PLoS One. 2017 Oct 24;12(10):e0186891. d
    Insert
    KanR
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    cat
    Available since
    March 5, 2020
    Availability
    Academic Institutions and Nonprofits only
    135190-plasmid-map-sequence-id-273774

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  14. ZJOM144

    Plasmid
    #133630
    Purpose
    Integration of SS-GFP-HDEL in genome, use auxotrophic marker URA3(Saccharomyces cerevisiae) Marker for yeast endoplasmic reticulum (ER).
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    pTPI1-SS-GFP-HDEL
    Use
    Tags
    EGFP
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Feb. 12, 2020
    Availability
    Academic Institutions and Nonprofits only
    133630-plasmid-map-sequence-id-269548

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  16. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  17. pNAS1B split mCherry N-ME(pS)VD/TRAP(RK)-C

    Plasmid
    #61966
    Purpose
    Positive control duet plasmid for SepOTS-dependent split mCherry assembly assay (i.e., N-mCherry is fused to the peptide ME(pS)VD (pS = phosphoserine); C-mCherry is fused to TRAP(RK))
    Depositor
    Lynne Regan
    Article
    Sawyer et al ACS Chem Biol. 2014 Nov 21;9(11):2502-7.
    Inserts
    N-mCherry-ME(pS)VD
    TRAP(RK)-C-mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    PBAD and PLtetO
    Available since
    Feb. 17, 2015
    Availability
    Academic Institutions and Nonprofits only
    61966-plasmid-map-sequence-id-101265

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  18. pM1s3ATScsg-Etag

    Plasmid
    #137946
    Purpose
    E. coli Nissle pMUT1-derived plasmid with ampicillin resistance and temperature sensitive curli expression
    Depositor
    Neel Joshi
    Article
    Kan et al ACS Synth Biol. 2020 Dec 10. doi: 10.102
    Inserts
    Synthetic curli operon
    Synthetic curli operon
    Use
    Synthetic Biology; For use with e. coli nissle in…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    pTlpA36
    Available since
    April 27, 2021
    Availability
    Academic Institutions and Nonprofits only
    137946-plasmid-map-sequence-id-331396

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  24. pTF-lacZ-rfp

    Plasmid
    #153037
    Purpose
    CRISPR-Cas12a genome editing in E. coli. Contains a constitutively CRISPR array targeting the lacZ gene in E. coli genome and donor DNA to insert an constitutively expressed rfp reporter gene.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153037-plasmid-map-sequence-id-318855

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  28. pMARO

    Plasmid
    #178086
    Purpose
    Derivative of pMK2010 that contains tetracycline resistance insert between attL1 and attL2 flanked by EcorV. Suitable as control or backbone for library construction for in vivo Gateway reaction.
    Depositor
    Sören Abel
    Article
    Gillman et al Plasmid. 2021 Nov;118:102608. doi: 10.10
    Insert
    TcR (tetA/tetR)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Tetracycline resistance cassette is flanked by Ec…
    Promoter
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178086-plasmid-map-sequence-id-349998

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  29. pHmK-C1

    Plasmid
    #87866
    Purpose
    Backbone plasmid for expressing pHluorin-mKate2-fusion protein in mammalan cells, which contains multi-cloning site like pEGFP-C1
    Depositor
    Isei Tanida
    Article
    Tanida et al PLoS One. 2014 Oct 23;9(10):e110600. doi
    Insert
    pHluorin-mKate2 fusion
    Use
    Tags
    pHluorin-mKate2 fusion
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 2, 2019
    Availability
    Academic Institutions and Nonprofits only
    87866-plasmid-map-sequence-id-252335

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Showing: 8611 - 8640 of 21422 results