We narrowed to 830 results for: Hr

1. pLVTHM_mePOU_3xFlag

   Plasmid

   #206395

   Purpose

   Expresses 3xflag fused mouse ePOU in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28 mePOU-POU

   Plasmid

   #206392

   Purpose

   Expresses the DNA binding domain of the ePOU with a 6xHis tag for protein purification

   Depositor

   Ralf Jauch

   Article

   Tan et al Mol Biol Evol. 2021 Jun 25;38(7):2854-28

   Insert

   ePOU
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   POU DNA binding domain only

   Promoter

   Available since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28a LZ control-FLAG

   Plasmid

   #199678

   Purpose

   Bacterial expression of Leucine Zipper (LZ) control-Flag

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Leucine zipper control
   

   Use

   Tags

   6xHis and Flag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330A-Yap1-E1-#2

   Plasmid

   #171519

   Purpose

   deletion of a genomic locus in Yap1 gene

   Depositor

   Hans Schöler

   Article

   Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

   Insert

   Yap1 (Yap1 Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pDONOR-SNCAe2-WT

   Plasmid

   #85845

   Purpose

   Donor plasmid for SNCA exon2 wild type sequence. Also contains TagBFP and EGFP

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   SNCA exon 2 homology arms (SNCA Human)
   

   Use

   Tags

   -

   Expression

   Bacterial and Mammalian

   Mutation

   -

   Promoter

   Available since

   March 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. FLAG-SENP2 NPm

   Plasmid

   #126593

   Purpose

   Expresses siRNA resistant SENP2 (nuclear pore mutant) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 1-65 and L329A/L331A in t…

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. FLAG-SENP2 CCm

   Plasmid

   #126594

   Purpose

   Expresses siRNA resistant SENP2 (coiled-coil deletion) in mammalian cells, Dox inducible in TetR cell lines

   Depositor

   Joanna Morris

   Article

   Garvin et al Genes Dev. 2019 Mar 1;33(5-6):333-347. d

   Insert

   SENP2 (SENP2 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Deletion of amino acids 203-228 and siRNA resista…

   Promoter

   CMV

   Available since

   Oct. 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMXs_FLAG-Sfxn1-3 (CG11739)

   Plasmid

   #110642

   Purpose

   Retroviral expression of Flag-tagged Drosophila CG11739 in mammalian cells

   Depositor

   David Sabatini

   Article

   Kory et al Science. 2018 Nov 16;362(6416). pii: 362

   Insert

   CG11739 (CG11739 Fly)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   codon-optimized cDNA (no amino acid mutations)

   Promoter

   Available since

   Nov. 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDONOR-SNCAe3-A53T

   Plasmid

   #85848

   Purpose

   Donor plasmid for SNCA exon3 A53T sequence. Also contains EGFP and tagBFP

   Depositor

   Jens Schwamborn

   Article

   Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

   Insert

   SNCA exon 3 homology arms (SNCA Human)
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   Available since

   May 30, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pJC20cdc8.27

    Plasmid

    #99362

    Purpose

    Bacterial expression vector containing cDNA encoding for the temperature sensitive fission yeast tropomyosin mutant, Cdc8.27.

    Depositor

    Dan Mulvihill

    Article

    Johnson et al Biochem Biophys Res Commun. 2017 Oct 25.

    Insert

    cdc8 (cdc8 Fission Yeast)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Glutamic acid 129 to Lysine

    Promoter

    T7

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEvol-pAzFRS.2.t1

    Plasmid

    #73546

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of several non-standard amino acids, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.2.t1
    pAzFRS.2.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEvol-pAzFRS.1.t1

    Plasmid

    #73547

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of p-azido-l-phenylalanine, into proteins in E coli in response to the amber codon, TAG

    Depositor

    Farren Isaacs

    Article

    Amiram et al Nat Biotechnol. 2015 Dec;33(12):1272-127

    Inserts

    pAzFRS.1.t1
    pAzFRS.1.t1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100X

    Plasmid

    #192148

    Purpose

    T cell CRISPR AAV vector

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLJM1-FLAG-GFP-OSBP

    Plasmid

    #134659

    Purpose

    Flag and GFP-tagged OSBP

    Depositor

    Roberto Zoncu

    Article

    Lim et al Nat Cell Biol. 2019 Oct;21(10):1206-1218

    Insert

    OSBP (OSBP Human)
    

    Use

    Lentiviral

    Tags

    FLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSLQ10878

    Plasmid

    #183964

    Purpose

    CAG-hyperCas12a(nuclease active)-HA-P2A-mCherry-T2A-HygroR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Insert

    HyperdCas12a
    

    Use

    CRISPR

    Tags

    HA, Hygromycin Resistance, and mCherry

    Expression

    Mammalian

    Mutation

    D156R, E292R, D235R, D350R

    Promoter

    CAG

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pJet-CMV-IARS1-GFP

    Plasmid

    #212322

    Purpose

    EGFP-tagged IARS1

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    IARS (IARS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Wildtype

    Promoter

    CMV

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pJet-CMV-IARS1[W435C]-GFP

    Plasmid

    #212323

    Purpose

    EGFP-tagged IARS1 Trp435Cys

    Depositor

    Sabine Fuchs

    Article

    Kok et al Nucleic Acids Res. 2025 Jan 7;53(1):gkae

    Insert

    IARS (IARS1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Trp435cys

    Promoter

    CMV

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSLQ10873

    Plasmid

    #183961

    Purpose

    U6-SKSKSO(crRNA array)-CAG-hyperdCas12a-HA-miniVPR

    Depositor

    Stanley Qi

    Article

    Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

    Inserts

    HyperdCas12a
    poly-crRNA array targeting Sox2-Klf4-Oct4
    

    Use

    CRISPR

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D832A, D156R, E292R, D235R, D350R

    Promoter

    CAG and U6

    Available since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. p3E ubb polyA

    Plasmid

    #195977

    Purpose

    Gateway p3E vector

    Depositor

    Alexa Burger, Christian Mosimann

    Article

    Kemmler et al Development. 2023 Mar 28:dev.201531. doi

    Insert

    ubb:polyA (ubb Zebrafish)
    

    Use

    Other

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLX_TRC311 ETV4-L

    Plasmid

    #74982

    Purpose

    Constitutive ETV4 ORF, blasticidin selection

    Depositor

    William Hahn

    Article

    Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

    Insert

    ETV4 (ETV4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    S396N

    Promoter

    EF1a

    Available since

    July 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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