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pCS3-MT-BX/ARF65-132

Plasmid

#78763

Purpose

To overexpress ARF65-132 in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Mutation

65-132

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Article

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-SwitchON-sgCh2-2

Plasmid

#199637

Purpose

Tamoxifen-inducible expression of sgRNA control targeting intergenic region

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

N/A

Use

Lentiviral

Available Since

June 2, 2023

Availability

Academic Institutions and Nonprofits only

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HOXB4 (human) HA-TAT-tag pET

Plasmid

#8526

Depositor

Corey Largman

Article

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB4 (HOXB4 Human)

Tags

HA

Expression

Bacterial

Mutation

replace T7 and HIS tags of pET with an HA tag;

Available Since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
HH06-LP Clone 6 heavy chain

Plasmid

#192176

Purpose

Clone 6 heavy chain (HH06)

Depositor

Sidi Chen

Article

Peng et al Nat Commun. 2022 Mar 28;13(1):1638. doi:

Insert

Clone 6 heavy chain (HH06)

Expression

Mammalian

Mutation

NA

Available Since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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p221- purVSG117UTR

Plasmid

#59732

Purpose

Inserts a puromycin resistance gene and a VSG117 gene into the VSG221 expression site of T.brucei downstream of the expression site promoter.

Depositor

Gloria Rudenko

Article

Smith et al PLoS One. 2009 Oct 26;4(10):e7532. doi:

Insert

VSG117 flanked upstream and downstream by sequences homologous to the VSG221 expression site

Use

For homologous recombination into vsg221 expressi…

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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pCS2+ oDam_f_GFPoNLS

Plasmid

#85819

Purpose

iDamID plasmid. To express transiently the optimized E. coli Dam adenine methyltransferase fused to the nuclear-localized mmGFP via flexylinker.

Depositor

Joachim Wittbrodt

Article

Gutierrez-Triana et al Development. 2016 Oct 5. pii: dev.139261

Insert

oDam_f_GFPoNLS

Tags

oNLS (optimized nuclear localization signal) C te…

Expression

Bacterial, Insect, Mammalia…

Mutation

L122A. Codon optimization compatible to work in M…

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pGBT9-Hook2

Plasmid

#198524

Purpose

Expression of GAL4 DNA-binding domain (BD)-Hook2 fusion protein in yeast (yeast two-hybrid assays)

Depositor

Juan Bonifacino

Article

Mattera et al Mol Biol Cell. 2020 Apr 15;31(9):963-979

Insert

Hook2 (HOOK2 Human)

Tags

GAL4-DNA binding domain fragment

Expression

Yeast

Mutation

His488Gln substitution

Promoter

ADH1

Available Since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pBS-Squ5'3'

Plasmid

#20165

Depositor

Eric Wieschaus

Article

Martin et al Nature. 2009 Jan 22;457(7228):495-9. doi

Insert

spaghetti squash (squ, myosin II RLC) 5' UTR, ORF (without stop codon) and 3' UTR with multiple cloning site downstream (sqh Fly)

Expression

Bacterial

Available Since

April 14, 2009

Availability

Academic Institutions and Nonprofits only
pPKm-245

Plasmid

#90503

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - P2A - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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sTR060

Plasmid

#177370

Purpose

Trigger RNA (GCAGGGATAAACGAGATAGATAAGATAAGA) that pairs with the toehold region in sTR056 (Addgene plasmid #177369) to restore translational activity.

Depositor

Jerome Bonnet, Jean-Loup Faulon

Article

Batista et al ACS Synth Biol. 2022 Jan 16. doi: 10.102

Insert

Trigger RNA

Use

Synthetic Biology; Cell-free protein synthesis

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPKm-244

Plasmid

#90502

Purpose

Expresses HO1, PCYA, FD and FNR, required for PCB synthesis. pSIN - EF-1alpha - MTS - tHO1 - P2A - MTS - tPCYA - IRES - MTS - tFD - P2A - MTS - tFNR

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

mito-tHO1, mito-tPCYA, mito-tFD, mito-tFNR

Use

Lentiviral

Available Since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pmiR-96 cluster promoter

Plasmid

#62517

Purpose

microRNA 183-96-182 cluster promoter luciferase plasmid

Depositor

Bharat Ramratnam

Article

Tang et al Nucleic Acids Res. 2014 Mar;42(5):2988-9

Insert

promoter of microRNA-183-96-182 cluster

Tags

RenSP (optimized renilla luciferase gene)

Expression

Mammalian

Available Since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only
pQC hKGA.wt V5-His IRES G418

Plasmid

#110390

Purpose

γ-Retroviral transfer vector for expressing glutminase isoform KGA (wild type), C-terminal V5-6xHis tags, IRES-driven Geneticin selection.

Depositor

Sandra Martha Gomes Dias

Insert

KGA glutaminase (GLS Human)

Use

Retroviral

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shId2 #2

Plasmid

#83090

Purpose

Lentiviral shRNA vector for inducible knockdown of mouse Id2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shId2 (Id2 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pBABE hygro MEN1 L22R

Plasmid

#11021

Depositor

Matthew Meyerson

Article

Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

Insert

MEN1 L22R (MEN1 Human)

Use

Retroviral

Expression

Mammalian

Mutation

L22R

Available Since

Dec. 2, 2005

Availability

Academic Institutions and Nonprofits only
pPN098

Plasmid

#91625

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pPN099

Plasmid

#91626

Purpose

Express sgRNA targeting human HCN1

Depositor

Lindy Barrett

Article

Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

Insert

HCN1 (HCN1 Human)

Use

CRISPR

Promoter

U6

Available Since

Oct. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-sgCCR5

Plasmid

#83930

Purpose

Lentiviral vector expressing an sgRNA targeting CCR5 NOTE: This plasmid does NOT contain Cas9.

Depositor

David Sabatini

Article

Park et al Nat Genet. 2016 Dec 19. doi: 10.1038/ng.

Insert

sgCCR5

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pFLAG-4S Tetherin

Plasmid

#41073

Depositor

Paul Spearman

Article

Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

Insert

Tetherin (BST2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

S74, S84, 137S, and 144S

Promoter

CMV

Available Since

Oct. 30, 2012

Availability

Academic Institutions and Nonprofits only