We narrowed to 16,140 results for: nans

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pSEVA221rAPOBEC1-T7RNAP

Plasmid

#167979

Purpose

Expresses fusion protein rAPOBEC1-T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Article

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

rAPOBEC1-T7RNAP (T7p07 Synthetic)

Tags

T7 tag and T7RNAP

Expression

Bacterial

Promoter

TetR-Ptet

Available Since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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CBP-HAT-GST

Plasmid

#21093

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Tags

GST

Expression

Bacterial

Mutation

HAT domain: aa 1196-1718

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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Hygro-iDEST

Plasmid

#75339

Purpose

GATEWAY destination vector for site-specific integration into the human AAVS1/PPP1R12C locus for doxycycline-inducible gene over-expression

Depositor

Danwei Huangfu

Article

Zhu et al Cell Stem Cell. 2016 Apr 27. pii: S1934-

Insert

TRE-DEST

Expression

Mammalian

Promoter

TRE-tight

Available Since

May 24, 2016

Availability

Academic Institutions and Nonprofits only

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pMT451

Plasmid

#139391

Purpose

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4), pNBU1 backbone, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

Bile Acid inducible sgRNA-4 with PM4 driving Nanoluc expression (NOT Gate 4)

Use

Synthetic Biology

Promoter

BA inducible promoters

Available Since

April 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLARRYv2-EGFP-T2A-Flpo

Plasmid

#233212

Purpose

Cloning expressed barcodes at the EcoRV site for preparing lentiviral libaries for single-cell lineage tracing experiments, with EGFP-T2A-Flpo for selection

Depositor

Alejo Rodriguez-Fraticelli

Article

Singh et al Cell Stem Cell. 2025 Feb 19:S1934-5909(2

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG-Flp-DOG

Plasmid

#80084

Purpose

Flp recombinase dependent on GFP (Flp-DOG) for expression in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

Insert

Flp-DOG

Expression

Mammalian

Promoter

CAG

Available Since

July 22, 2016

Availability

Academic Institutions and Nonprofits only

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pTS93 pHAGE2 CMVtetO2 GFP-22xGS-MCS

Plasmid

#199346

Purpose

Lentiviral transfer plasmid for doxycycline inducible expression of a N-terminally GFP-tagged protein in mammalian cells.

Depositor

Rebecca Voorhees

Article

Stevens et al Nat Protoc. 2024 Jan;19(1):127-158. doi:

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβεζ

Plasmid

#187353

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 (epsilon, and zeta)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Tags

6His and myc

Expression

Bacterial

Promoter

pBAD

Available Since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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His-Tev-WWP2

Plasmid

#186807

Purpose

Codon-optimized bacterial expression plasmid. Expresses ubiquitin ligating enzyme His-Tev-WWP2.

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

His-Tev-WWP2 (WWP2 Human)

Tags

His6-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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pSb_init containing anti SARS-CoV-2_RBD sybody Sb#16

Plasmid

#153524

Purpose

Expression of the anti SARS-CoV-2_RBD sybody Sb#16

Depositor

Markus Seeger

Article

Walter et al bioRxiv 2020.04.16.045419

Insert

Sb#16

Expression

Bacterial

Promoter

pBAD

Available Since

June 1, 2020

Availability

Academic Institutions and Nonprofits only

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pAB078d5

Plasmid

#79204

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1) luxAB; Ppro1 434cI

Expression

Bacterial

Promoter

PlacZ-opt (OR1); Ppro1

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-p65AD-GBP6

Plasmid

#49440

Purpose

Expression of a GFP-dependent transcription factor component in mammalian cells

Depositor

Connie Cepko

Article

Tang et al Cell. 2013 Aug 15;154(4):928-39. doi: 10

Insert

GBP6-p65 transactivation domain fusion protein

Expression

Mammalian

Promoter

CAG

Available Since

Jan. 9, 2014

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-CDAmax-BlastR

Plasmid

#152995

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

CDA-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pJBL711

Plasmid

#140381

Purpose

Expresses OtrR protein under the control of T7-lacO promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-lacO-OtrR-T

Tags

TEV-6XHis

Expression

Bacterial

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3 HA Sam68 deltaKH

Plasmid

#17688

Depositor

David Shalloway

Article

Lin et al J Biol Chem. 1997 Oct 24. 272(43):27274-

Insert

Sam68 (Khdrbs1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

aa 157-256 deleted (KH domain)

Available Since

April 24, 2008

Availability

Academic Institutions and Nonprofits only
pCS2+_Flag-myc-NES-Tir1

Plasmid

#117717

Purpose

mRNA synthesis of Flag-myc-NES-TIR1 for injection

Depositor

Joerg Mansfeld

Article

Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

Insert

TRANSPORT INHIBITOR RESPONSE 1

Tags

Flag-Myc-NES

Expression

Mammalian

Mutation

codon optimized TIR1 for murine expression

Promoter

SP6

Available Since

Dec. 4, 2018

Availability

Academic Institutions and Nonprofits only

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p3xFLAG-Tmprss6(S762A)

Plasmid

#18791

Depositor

Bruce Beutler

Article

Du et al Science. 2008 May 23. 320(5879):1088-92.

Insert

Type II transmembrane serine protease 6 (Tmprss6 Mouse)

Tags

3xFLAG

Expression

Mammalian

Mutation

Changed Serine762 to Alanine

Available Since

July 14, 2008

Availability

Academic Institutions and Nonprofits only
pHRSin_hTCRαγε

Plasmid

#187352

Purpose

Lentiviral expression of GPa3b17 hTCR alpha, and truncated hCD3 (gamma, and epsilon)

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR alpha, and truncated hCD3 (gamma, and epsilon)

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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pHRSin_hTCRβε

Plasmid

#187354

Purpose

Lentiviral expression of GPa3b17 hTCR beta, and truncated hCD3 epsilon

Depositor

Simon Davis

Article

Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

Insert

GPa3b17 hTCR beta, and truncated hCD3 epsilon

Use

Lentiviral

Promoter

SFFV

Available Since

Sept. 29, 2022

Availability

Academic Institutions and Nonprofits only

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