167,212 results

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pcDNA3.1(+) K-Ras(G12C)

Plasmid

#224284

Purpose

Cell transfection and expression of K-Ras (G12C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

K-Ras (KRAS Human)

Expression

Mammalian

Mutation

G12C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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EC67907

Plasmid

#211794

Purpose

Over expresses Cas9 and GR-GRF-GIF. Must receive guide cassettes

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Research Square, 12 March 2024, PREPRINT

Insert

HptII, Cas9, GR-GRF-GIF

Expression

Plant

Available Since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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p2710-pHAGE2-Ef1aL-wtYAP-UBC-tagBFP-loxP

Plasmid

#216479

Purpose

lentiviral dual expression of wtYAP (YAP1) and tagBFP with loxP site for Cre excision

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

wtYAP (YAP1) (YAP1 Human)
tagBFP

Use

Cre/Lox and Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pWUR790

Plasmid

#101723

Purpose

Synthetic codon optimized P. furiosus ago with N-terminal Strep(II)-tag in pCDF-1b vector, expression vector for PfAgo

Depositor

John van der Oost

Article

Swarts et al Nucleic Acids Res. 2015 May 26;43(10):51

Insert

PfAgo

Tags

Strep(II)-tag

Expression

Bacterial

Promoter

T7 Promotor

Available Since

Nov. 27, 2017

Availability

Academic Institutions and Nonprofits only

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Ntn1-Fc-His

Plasmid

#72104

Purpose

Expresses the entire Netrin 1 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

Depositor

Woj Wojtowicz

Article

Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

Insert

Ntn1 (Ntn1 Mouse)

Tags

Fc-His

Expression

Mammalian

Promoter

CMV

Available Since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pNDL-60

Plasmid

#161720

Purpose

In vitro transcription construct for production of cytosolic acvr2b-CIB1N mRNA

Depositor

Adam Cohen

Article

McNamara et al bioRxiv [Preprint]. 2024 Apr 12:2024.04.

Insert

cytosolic acvr2b-CIB1N (acvr2b Zebrafish)

Use

In vitro transcription

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pAi14-GFPNLS-MC

Plasmid

#87114

Purpose

minicircle parental backbone plasmid including GFPNLS-pA knock-in donor and one cutting site for HITI

Depositor

Juan Belmonte

Article

Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

Insert

GFPNLSPpA

Use

CRISPR

Promoter

nEF

Available Since

March 31, 2017

Availability

Academic Institutions and Nonprofits only

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ABEmax-Puro V3

Plasmid

#226956

Purpose

ABEmax plasmid enabling A:T to G:C base editing using a single plasmid. sgRNA uses 3TC scaffold for increased sgRNA expression.

Depositor

Paul Thomas

Article

Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

March 10, 2025

Availability

Academic Institutions and Nonprofits only

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pYL-kasOp-FnCas12a2

Plasmid

#177874

Purpose

Expresses Streptomyces codon optimized FnCas12a. Used to modify genome of Streptomyces.

Depositor

Yunzi Luo

Article

Zhang et al Front Bioeng Biotechnol. 2020 Jun 30;8:7

Insert

Streptomyces codon optimized FnCas12a

Use

CRISPR

Promoter

kasOp*

Available Since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSCAC-69

Plasmid

#31241

Purpose

Mitochondrial localization of EGFP, for real-time imaging of mitochondria in transgenic zebrafish

Depositor

Seok-Yong Choi

Article

Kim et al Biotechniques. 2008 Sep . 45(3):331-4.

Insert

mitochondrial localization enhanced green fluorescent protein

Available Since

June 3, 2016

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-STX17ΔNTD

Plasmid

#89941

Purpose

Expresses syntaxin17 lacking N-terminal domain tagged with GFP in mammalian cells

Depositor

Noboru Mizushima

Article

Uematsu et al Autophagy. 2017 Aug 3;13(8):1452-1464. d

Insert

human syntaxin17 lacking N-terminal domain (STX17 Human)

Use

Retroviral

Tags

GFP

Mutation

deleted amino acids 1-161

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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mCherry-Integrin-Beta1-N-18

Plasmid

#55064

Purpose

Localization: Integrin/Focal Adhesions, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Integrin-Beta1 (ITGB1 Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 His-V5 Myc

Plasmid

#176049

Purpose

Expresses Myc tagged with His and V5 in Mammalian cells

Depositor

Linda Penn

Article

Tu et al Cancer Cell. 2018 Oct 8;34(4):579-595.e8

Insert

Myc

Tags

6xHis and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV_CreNLS_PP7

Plasmid

#232439

Purpose

expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

Depositor

Gil Westmeyer

Article

Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

Insert

Cre–MYC-NLS

Expression

Mammalian

Available Since

April 10, 2025

Availability

Academic Institutions and Nonprofits only

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pAG241 FKBP-CFAST10

Plasmid

#130814

Purpose

Expresses FKBP-CFAST10 in mammalian cells

Depositor

Arnaud Gautier

Article

Tebo et al Nat Commun. 2019 Jun 27;10(1):2822. doi:

Insert

FKBP-CFAST10

Tags

Myc-tag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEXP5-aHL_spytag_his

Plasmid

#107334

Purpose

Monomeric SpyTag-6×-His alpha-hemolysin

Depositor

George Church

Article

Stranges et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

Insert

alpha-hemolysin-SpyTag-6xHis

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pTP955

Plasmid

#104164

Purpose

Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (3x Cysteines)

Depositor

Dirk Görlich

Article

Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

Insert

Anti-rabbit IgG Fc specific nanobody TP897 (3xCysteines)

Tags

14xHistidine tag and NEDD8 from Brachypodium dist…

Expression

Bacterial

Available Since

Dec. 21, 2017

Availability

Academic Institutions and Nonprofits only

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pICH41722

Plasmid

#48016

Depositor

Sylvestre Marillonnet

Article

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

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Gq-CASE

Plasmid

#168125

Purpose

Encodes a BRET-based activity sensor for the heterotrimeric G protein Gq. Composed of the subunits G alpha q (GNAQ) tagged with NanoLuciferase, G beta 3 (GNB3) and Venus-tagged G gamma 9 (GNG9).

Depositor

Gunnar Schulte

Article

Schihada et al Sci Signal. 2021 Sep 7;14(699):eabf1653.

Insert

GNAQ, GNB3, GNG9 (GNAQ, GNB3, GNGT2 Human)

Use

Luciferase

Tags

cpVenus on GNG9

Expression

Mammalian

Mutation

NLuc is inserted at F124/E125 within GNAQ

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-LacR-TopBP1

Plasmid

#31317

Depositor

Aziz Sancar

Article

Lindsey-Boltz et al J Biol Chem. 2011 Jun 3. 286(22):19229-3

Insert

TopBP1 (TOPBP1 Human)

Tags

FLAG and LacR

Expression

Mammalian

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only

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