We narrowed to 11,977 results for: pcDNA

1. pcDNA3-Venus-Venus-FLARE-Cameleon

   Plasmid

   #123351

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3-K27 histone methylation reporter

   Plasmid

   #22865

   Depositor

   Alice Ting

   Article

   Lin et al J Am Chem Soc. 2004 May 19. 126(19):5982

   Insert

   K27 HMR
   

   Use

   Tags

   CFP and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pcDNA3-Lyso-mCherry-Rheb(C181S)

   Plasmid

   #192438

   Purpose

   Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant targeted to lysosome membrane.

   Depositor

   Jin Zhang

   Article

   Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1

   Insert

   Lyso-mCherry-Rheb(C181S) (RHEB Human)
   

   Use

   Tags

   Full-length LAMP1 and mCherry

   Expression

   Mammalian

   Mutation

   Rheb Cys 181 mutated to Ser; abolishes C-terminal…

   Promoter

   CMV

   Available since

   Dec. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCDNA3.1-Flag-H2A K13-15R

   Plasmid

   #63563

   Purpose

   Expression of human H2A with K13R and K15R tagged with Flag

   Depositor

   Titia Sixma

   Article

   Mattiroli et al Cell. 2012 Sep 14;150(6):1182-95. doi: 1

   Insert

   H2A (H2AC20 Human)
   

   Use

   Tags

   Flag (MDYKDDDDKVPRIQ)

   Expression

   Mammalian

   Mutation

   Lysine 13 and Lysine 15 are now Arginine

   Promoter

   CMV

   Available since

   March 13, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pcDNA3-GFP-LpIA(wild-type)

   Plasmid

   #61821

   Purpose

   Encodes a wild-type sequence of LplA and serves as the negative control for resorufin labeling

   Depositor

   Alice Ting

   Article

   Liu et al Proc Natl Acad Sci U S A. 2014 Oct 28;11

   Insert

   E. coli lipoic acid ligase
   

   Use

   Tags

   6XHis, EGFP, and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-FKBP-(GGS)X6-HaloTag C58

   Plasmid

   #171428

   Purpose

   Expression of FKBP-(GGS)X6-HaloTag C58 in mammalian cells

   Depositor

   Yujie Sun

   Article

   Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

   Insert

   FKBP-(GGS)X6-HaloTag C58
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3-Lyn-dPlcR(K30/32L)

   Plasmid

   #181833

   Purpose

   Phospholipid binding-deficient Lyn-dPlcR negative-control plasmid.

   Depositor

   Jin Zhang

   Article

   Hertel et al ACS Chem Biol. 2020 Jan 17;15(1):33-38.

   Insert

   Lyn-dPlcR (K30L,K32L)
   

   Use

   Tags

   N-terminal targeting sequence from Lyn kinase, dd…

   Expression

   Mammalian

   Mutation

   Lysines 30 and 32 in PH domain mutated to leucine.

   Promoter

   CMV

   Available since

   June 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1(+) ZKSCAN1 MCS-ciRS7 Exon

   Plasmid

   #69906

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCDNA3-Myc3-Cul3 delta N41

   Plasmid

   #21590

   Depositor

   Yue Xiong

   Article

   Furukawa et al Mol Cell Biol. 2005 Jan . 25(1):162-71.

   Insert

   cullin 3 (CUL3 Human)
   

   Use

   Tags

   myc3

   Expression

   Mammalian

   Mutation

   Residues 34-74 deleted from N terminus.

   Promoter

   Available since

   July 1, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.2/DEST/hCx43-E2HA-stop

    Plasmid

    #40909

    Depositor

    Robin Shaw

    Article

    Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

    Insert

    Connexin 43 internal HA tag (GJA1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HA tag inserted in second extracellular loop

    Promoter

    CMV

    Available since

    Nov. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA5/FRT/TO-Venus-Flag (865)

    Plasmid

    #40998

    Depositor

    Jonathon Pines

    Article

    Sedgwick et al EMBO J. 2013 Jan 23;32(2):303-14. doi: 1

    Type

    Empty backbone

    Use

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK) and Venus YFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 8, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pcDNA3-3myc-6His-EZH2 21D

    Plasmid

    #42664

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21D

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA-GalNAc-T2-GFP-ESCargo(FTV)

    Plasmid

    #140163

    Purpose

    Expresses a regulatable secretory cargo for mammalian cells and a Golgi marker

    Depositor

    Benjamin Glick

    Article

    Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

    Insert

    GalNAc-T2-msGFP2 and piGH-FTVNTT-DsRed-Express2-FKBP(LV)(C22V) (GALNT2 Human, Synthetic)
    

    Use

    Tags

    ER signal sequence (MGWSCIILFLVATATGAHS) (N termi…

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA5-3xFLAG-SRSF1 siRNA-resitant

    Plasmid

    #218974

    Purpose

    Vector for expressing siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGGAGUUUGUACGGAAA)

    Depositor

    Thomas Gonatopoulos-Pournatzis

    Article

    Xiao et al Mol Cell. 2024 Jul 11;84(13):2553-2572.e

    Insert

    SRSF1 cDNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    siRNA-resistant SRSF1 cDNA (siRNA sequence: CGUGG…

    Promoter

    CMV

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 SP-His-mCherry-HRP-VhHGFP

    Plasmid

    #112157

    Purpose

    Expression and secretion of anti-GFP FLIPPER-bodies, small protein probes for correlated microscopy. FLIPPER-bodies are the fusion of a nanobody with a fluorescent protein and a peroxidase.

    Depositor

    Ben Giepmans

    Article

    de Beer et al Histochem Cell Biol. 2018 Mar;149(3):261

    Insert

    anti-GFP FLIPPER-body
    

    Use

    Tags

    His and Thrombin cleavage site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3-3myc-6His-EZH2 21A

    Plasmid

    #42663

    Depositor

    Mien-Chie Hung

    Article

    Cha et al Science. 2005 Oct 14;310(5746):306-10.

    Insert

    EZH2 (EZH2 Human)
    

    Use

    Tags

    3Myc and 6His

    Expression

    Mammalian

    Mutation

    S21A

    Promoter

    Available since

    July 17, 2013

    Availability

    Academic Institutions and Nonprofits only

17. pcDNA5-FRT-GFP-mCherry-3pGW

    Plasmid

    #53965

    Purpose

    Bidirectional construct in the pcDNA5/FRT backbone with GFP and mCherry driven by the same CMV promoter along with a Gateway cloning site downstream mCherry

    Depositor

    Saeed Tavazoie

    Article

    Oikonomou et al Cell Rep. 2014 Apr 10;7(1):281-92. doi:

    Inserts

    GFP
    mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 22, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3-myc-his-BACH1 P47A

    Plasmid

    #17644

    Depositor

    Ronny Drapkin

    Article

    Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

    Insert

    BACH1 (BRIP1 Human)
    

    Use

    Tags

    His and Myc

    Expression

    Mammalian

    Mutation

    To generate the tumor-associated mutant, the prol…

    Promoter

    Available since

    April 4, 2008

    Availability

    Academic Institutions and Nonprofits only

19. pcDNA3.1 CARD8-TEV-GFP-FLAG

    Plasmid

    #169991

    Purpose

    Mammalian purification construct for CARD8

    Depositor

    Daniel Bachovchin

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Use

    Tags

    TEV-GFP-FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3-Flag mTOR S2035T D2357E

    Plasmid

    #26605

    Depositor

    Jie Chen

    Article

    Vilella-Bach et al J Biol Chem. 1999 Feb 12. 274(7):4266-72

    Insert

    mTOR (MTOR Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    S2035T D2357E

    Promoter

    Available since

    Feb. 1, 2011

    Availability

    Academic Institutions and Nonprofits only