159,557 results

1. pTECH-MbPylRS(IPYE)

   Plasmid

   #104072

   Purpose

   Expression plasmid encoding MbPylRS(IPYE) and pylT

   Depositor

   David Liu

   Article

   Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

   Insert

   Plpp MbPylRS(IPYE); PproK pyl
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Contains activating mutations V31I, T56P, H62Y, A…

   Promoter

   Plpp and PproK

   Available since

   Dec. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMX-eSOX17FNV

   Plasmid

   #118838

   Purpose

   Retroviral expression of a mutant of Sox17 that improves efficiency of iPSC generation

   Depositor

   Ralf Jauch

   Article

   Veerapandian et al Stem Cell Reports. 2018 Aug 14;11(2):593

   Insert

   eSOX17FNV (Sox17 Mouse)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   FNV: L46F, V53N, E57V

   Promoter

   Available since

   April 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAG_PCP-T5Exo

   Plasmid

   #214736

   Purpose

   encodes T5 exonuclease with a PP7 coat protein fused to the N terminus

   Depositor

   Gil Westmeyer

   Article

   Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4

   Insert

   CAG_PCP-T5Exo_bGH
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. GNRHR-Tango

   Plasmid

   #66297

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GNRHR (GNRHR Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

5. mCherry-pCAG-donor(R2Tg)-GFP(intron)

   Plasmid

   #223248

   Purpose

   Mammalian expression of the R2Tg RNA donor

   Depositor

   Wei Li

   Article

   Chen et al Cell. 2024 Jul 1:S0092-8674(24)00694-9.

   Insert

   R2Tg donor
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. p35S:Citrine-TurboID-3xHA

   Plasmid

   #199243

   Purpose

   This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

   Depositor

   Savithramma Dinesh-Kumar

   Article

   Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

   Insert

   Citrine-TurboID
   

   Use

   Tags

   3xHA and TurboID-3xHA

   Expression

   Plant

   Mutation

   Promoter

   CaMV 35S

   Available since

   June 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBBK18 pCAS-Tyr-[gRNA: 1=FF18] (SplitHygR, AmpR)

   Plasmid

   #179002

   Purpose

   Sp.Cas9 and gRNA yeast expression vector with FF18 gRNA pre-cloned. Selection =SplitHygromycin resistance

   Depositor

   Vincent Martin

   Article

   Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

   Insert

   S. pyogenes Cas9
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMBIS

   Plasmid

   #17817

   Depositor

   Jay Keasling

   Article

   Martin et al Nat Biotechnol. 2003 Jul . 21(7):796-802

   Insert

   mevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 27, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSK9065

   Plasmid

   #84345

   Purpose

   tightly controlled expression of mRFP fusion proteins in S. aureus

   Depositor

   Neville Firth

   Article

   Brzoska et al Appl Environ Microbiol. 2013 May;79(9):3

   Type

   Empty backbone

   Use

   Tags

   mRFPmars

   Expression

   Bacterial

   Mutation

   Promoter

   Pxyl/tet

   Available since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1(+)-Lifeact-(GGS)X6-HaloTag C58

    Plasmid

    #171435

    Purpose

    Expression of Lifeact-(GGS)X6-HaloTag C58 in mammalian cells

    Depositor

    Yujie Sun

    Article

    Shao et al Commun Biol. 2021 Mar 19;4(1):378. doi:

    Insert

    Lifeact-(GGS)X6-HaloTag C58
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-11b_PcrA_M6_H93A

    Plasmid

    #194156

    Purpose

    Engineered PcrA_M6 helicase to support isothermal amplification method SHARP

    Depositor

    Taekjip Ha

    Article

    Gavrilov et al Nat Commun. 2022 Oct 23;13(1):6312. doi:

    Insert

    Engineered helicase PcrA_M6
    

    Use

    Tags

    6x His tag and FLAG tag

    Expression

    Bacterial

    Mutation

    H93A, C96A, N187C, C247A, L384V, L409C

    Promoter

    Available since

    Jan. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pYFP-ErbB2

    Plasmid

    #66948

    Purpose

    Mammalian expression of rat ErbB2 tagged with YFP

    Depositor

    Bo van_Deurs, Mads Lerdrup

    Article

    Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

    Insert

    ErbB2 (Erbb2 Rat)
    

    Use

    Tags

    6xHis, V5 Tag, and YFP

    Expression

    Mammalian

    Mutation

    (See depositor comment below on mutations A24T an…

    Promoter

    CMV

    Available since

    Sept. 15, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pQCH

    Plasmid

    #164160

    Purpose

    IncQ BHR plasmid

    Depositor

    Calvin Henard

    Article

    Tapscott et al Appl Environ Microbiol. 2019 May 16;85(1

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP-C1-Fibrillarin

    Plasmid

    #26673

    Depositor

    Sui Huang

    Article

    Chen et al J Cell Biol. 2001 Apr 2. 153(1):169-76.

    Insert

    Fibrillarin (FBL Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 10, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiEGFPdestablized - EFS-EGFPd2PEST-2A-MCS-Hygro

    Plasmid

    #138152

    Purpose

    Expresses eGFP in mammalian cells, contains a 3' MCS. Localized to the nucleus

    Depositor

    Neville Sanjana

    Article

    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

    Insert

    eGFP-MCS
    

    Use

    Lentiviral

    Tags

    2PEST AND NLS

    Expression

    Mutation

    Promoter

    Available since

    Feb. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. BNLS

    Plasmid

    #50836

    Purpose

    Expresses a tandem ddFP heterodimer in mammalian cells, construct 1 for a translocation-based green fluorescent caspase-3 biosensor, used together with plasmid 1 (GANLS-DEVD-BNES or RANLS-DEVD-BNES).

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP B
    

    Use

    Tags

    triplicated NLS sequence DPKKKRKV

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. plex_307-ccdB-blasticidin

    Plasmid

    #201136

    Purpose

    pLEX_307 plasmid with blasticidin marker

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1α

    Available since

    June 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENN.AAV.CB7.CI.mCerulean.WPRE.RBG

    Plasmid

    #105557

    Purpose

    AAV expression of mCerulean from CB7 promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1 and AAV9

    Insert

    mCerulean
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CB7

    Available since

    May 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TFORF2923

    Plasmid

    #143674

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NFIA (NFIA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. piggyBac-rtTA (4th_Gen)-SNCA(A53T)-sfGFP-IRES-NGN2-puro (VK_1123)

    Plasmid

    #209080

    Purpose

    Ngn2 mediated cortical neuron induction, along with SNCA(A53T)-sfGFP over-expression

    Depositor

    Vikram Khurana

    Article

    Lam et al bioRxiv 2022.11.08.515615

    Inserts

    SNCA (SNCA Human)
    NGN2 (Neurog2 Mouse)
    

    Use

    Tags

    sfGFP

    Expression

    Mammalian

    Mutation

    A53T

    Promoter

    TRE4G

    Available since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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23. pCAG-mGFP

    Plasmid

    #14757

    Depositor

    Connie Cepko

    Article

    Matsuda et al Proc Natl Acad Sci U S A. 2007 Jan 16;10

    Has Service

    Cloning Grade DNA

    Insert

    membrane bound form of EGFP
    

    Use

    Tags

    Palmitoylation signal

    Expression

    Mammalian

    Mutation

    Promoter

    pCAG

    Available since

    April 20, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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24. pMCB306-GFP-Rab12

    Plasmid

    #198471

    Purpose

    lentiviral expression of GFP-Rab12

    Depositor

    Suzanne Pfeffer

    Article

    Dhekne et al Elife. 2023 Oct 24;12:e87098. doi: 10.75

    Insert

    GFP-Rab12 (RAB12 Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1A

    Available since

    April 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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25. pCAGGS Donor mClover-LMNA

    Plasmid

    #98970

    Purpose

    Donor for endogenous N-ter tagging of hLMNA with mClover. Cas9 Homologous Recombination Reporter

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA5'UTR-mClover-LMNA5'exon1 (LMNA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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26. pLVTHM

    Plasmid

    #12247

    Purpose

    2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmid

    Depositor

    Didier Trono

    Article

    Wiznerowicz et al J Virol. 2003 Aug . 77(16):8957-61.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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27. pTRE3G-Bi-α-chain

    Plasmid

    #133730

    Purpose

    Expresses the gePSI-α-chain and ZsGreen1 under control of the inducible bi-directional TRE3G promoter.

    Depositor

    Erin Schuman

    Article

    Heumuller et al Nat Methods. 2019 Jul 15. pii: 10.1038/s

    Inserts

    a-Chain_gePSI
    ZsGreen1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Only one part of the gePSI-system (aa1-141 + stop…

    Promoter

    pTRE3G-Bi

    Available since

    Dec. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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28. TFORF2873

    Plasmid

    #143648

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    ELF1 (ELF1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pExpreS2-1-C3-PA-10H-SIII

    Plasmid

    #175443

    Purpose

    HiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 Platform

    Depositor

    Wyatt Yue

    Article

    Coker et al Front Bioeng Biotechnol. 2022 May 5;10:8

    Type

    Empty backbone

    Use

    Tags

    3C-Protein A-10His-TwinStrep and BiP Secretion Pe…

    Expression

    Insect

    Mutation

    Promoter

    Fused Actin-HSP70

    Available since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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30. pJM253 (miniCTX1-rhaSR-PrhaBAD)

    Plasmid

    #110571

    Purpose

    Integration vector for Pseudomonas aeruginosa with rhaSR-PrhaBAD inducible promoter

    Depositor

    Joanna Goldberg

    Article

    Meisner et al Appl Environ Microbiol. 2016 Oct 27;82(2

    Insert

    rhaSR-PrhaBAD
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    rhaSR-PrhaBAD

    Available since

    May 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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