161,805 results

1. control (pGS701)

   Plasmid

   #160037

   Purpose

   pLV-A4-UCOE-EF1-3xHA-GFP-MCS-IRES-BLAS-2A-SNAP-WPRE-A2

   Depositor

   David Sabatini

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1

   Available since

   March 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcmv3-IRG1-HA

   Plasmid

   #198181

   Purpose

   Mammalian expression of HA-tagged human ACOD1 (IRG1)

   Depositor

   Yun Zhao

   Article

   Li et al Cell Rep. 2023 Mar 28;42(3):112145. doi:

   Insert

   ACOD1 (ACOD1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   HA tag is added to C-terminal of target protein

   Promoter

   CMV

   Available since

   April 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pcDNA3.1/cyto-iAspSnFR

   Plasmid

   #201394

   Purpose

   CMV overexpression of cyto-iAspSnFR in mammalian cell lines

   Depositor

   Kai Johnsson

   Article

   Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

   Insert

   cyto-iAspSnFR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

   Promoter

   CMV

   Available since

   May 31, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. mApple-Alpha-V-Integrin-25

   Plasmid

   #54866

   Purpose

   Localization: Focal Adhesions, Excitation: 568, Emission: 592

   Depositor

   Michael Davidson

   Insert

   Alpha-V Integrin (ITGAV Human)
   

   Use

   Tags

   mApple

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. GPR78-Tango

   Plasmid

   #66374

   Purpose

   Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation

   Depositor

   Bryan Roth

   Article

   Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-

   Insert

   GPR78 (GPR78 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLenti6.3 GCN1-3xFlag_IRES-iRFP

   Plasmid

   #198388

   Purpose

   GCN1 expression

   Depositor

   Jack Taunton

   Article

   Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

   Insert

   GCN1 (GCN1 Human)
   

   Use

   Lentiviral

   Tags

   3xFlag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. hAAVS1 1L TALEN

   Plasmid

   #35431

   Purpose

   TALEN designed to target the human AAVS1 locus

   Depositor

   Feng Zhang

   Article

   Sanjana et al Nat Protoc. 2012 Jan 5;7(1):171-92. doi:

   Insert

   hAAVS1 1L TALEN
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 17, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pALB-GFP

   Plasmid

   #55759

   Purpose

   Fluorescent marker for hepatocytic lineage

   Depositor

   Snorri Thorgeirsson

   Article

   Heo et al Hepatology. 2006 Dec;44(6):1478-86.

   Insert

   albumin enhancer/promoter (Alb Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Mouse Albumin Enhancer/Promoter

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMK289 (mAID-mClover-NeoR)

   Plasmid

   #72827

   Purpose

   C-terminal tagging with mAID-mClover using CRISPR/Cas9

   Depositor

   Masato Kanemaki

   Article

   Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

   Insert

   mAID-mClover-Neo
   

   Use

   CRISPR

   Tags

   mAID-mClover

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pKA-207I10

    Plasmid

    #79845

    Purpose

    Expresses NLS-PpsR2-VP16-IRES-RpBphP1-mCherry-TetR under CMV promoter

    Depositor

    Vladislav Verkhusha

    Article

    Kaberniuk et al Nat Methods. 2016 May 9. doi: 10.1038/nm

    Insert

    NLS-PpsR2-VP16-IRESI10-BphP1-mCherry-TetR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    RpPpsR2 cysteine 439 changed to serine

    Promoter

    CMV

    Available since

    Aug. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pNC01

    Plasmid

    #233643

    Purpose

    For bacterial expression of sfGFP c-terminally tagged with VitelloTagA.

    Depositor

    Zak Swartz

    Article

    Clarke et al Development. 2024 Oct 15;151(20):dev2028

    Insert

    sfGFP with c-terminal VitelloTagA
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 15, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Anti-6xHis [N144/14R]

    Plasmid

    #177500

    Purpose

    Mammalian Expression Plasmid of anti-6xHis. Derived from hybridoma N144/14.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    anti-6xHis recombinant mouse monoclonal antibody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Dual CMV

    Available since

    Jan. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. LeGO-C2

    Plasmid

    #27339

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pUC57-sgRNA expression vector

    Plasmid

    #51132

    Purpose

    For in vitro transcription of sgRNA from the T7 promoter.

    Depositor

    Xingxu Huang

    Article

    Shen et al Nat Methods. 2014 Mar 2. doi: 10.1038/nm

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AcrIIA2

    Plasmid

    #101042

    Purpose

    Anti-CRISPRprotein which inhibit SpyCas9 and LmoCas9 activity.

    Depositor

    Zhiwei Huang

    Article

    Dong et al Nature. 2017 Jun 15;546(7658):436-439. d

    Insert

    AcrIIA2
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Oct. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA3.1-Gαi*-BERKY3

    Plasmid

    #158198

    Purpose

    BRET biosensor for detection of Gαi-GTP in mammalian expression vector pcDNA3.1(+)

    Depositor

    Mikel Garcia-Marcos

    Article

    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30

    Insert

    Lyn11-Nluc-ER/K linker-3xYFP-KB1753-myc
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pCDF-GFPplus

    Plasmid

    #172718

    Purpose

    Expression of a fast folding GFP variant GFP plus

    Depositor

    Tilmann Weber

    Article

    Tong et al Nat Commun. 2021 Sep 1;12(1):5206. doi:

    Insert

    GFP plus
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA 3.1 KBTBD4 R313PRR

    Plasmid

    #184629

    Purpose

    Mammalian expression vector with N-terminal Flag for KBTBD4 R313PRR expression in mammalian cells

    Depositor

    Vincenzo D'Angiolella

    Article

    Chen et al Cell Death Differ. 2022 Apr 4. pii: 10.1

    Insert

    N-terminal 3xFlag tagged KBTBD4 R313PRR (KBTBD4 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R313PRR

    Promoter

    CMV

    Available since

    June 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. single polypeptide FPX biosensor for calcium ion

    Plasmid

    #60887

    Purpose

    FPX biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    single polypeptide FPX biosensor for calcium ion
    

    Use

    Tags

    HindIII site

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pJK645

    Plasmid

    #71700

    Purpose

    Produces E. coli thioredoxin (EcTrxA)

    Depositor

    T. J. Kappock

    Insert

    thioredoxin
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pENTR221-BNT162b2ΔUTR

    Plasmid

    #171214

    Purpose

    Gateway entry vector containing the BNT162b2/tozinameran cDNA sequence lacking the original 5' and 3' UTRs and the poly(A) tail. The original Kozak sequence has also been altered.

    Depositor

    Liang Wei Wang

    Article

    Low et al medRxiv 2021.04.27.21256151

    Insert

    BNT162b2ΔUTR
    

    Use

    Gateway cloning

    Tags

    Expression

    Mutation

    Kozak sequence changed from CCCGCCACC to GGCTGCAC…

    Promoter

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pKLV2-U6gRNA5(BbsI)-PGKpuro2AmCherry-W

    Plasmid

    #67977

    Purpose

    CRISPR gRNA expression vector with an improved scaffold and puro/mCherry markers

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    U6gRNA cassette, PGKpuro2AmCherry cassette, WPRE
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Deleted BbsI site within WPRE

    Promoter

    human U6 promoter, mouse PGK promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pBK-VRER-BE3

    Plasmid

    #85173

    Purpose

    Expresses VRER-BE3 in mammalian cells

    Depositor

    David Liu

    Article

    Kim et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Insert

    VRER-BE3
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. TriEx-4 DHX36

    Plasmid

    #68368

    Purpose

    Expresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.

    Depositor

    James Vaughn

    Article

    Vaughn et al J Biol Chem. 2005 Nov 18;280(46):38117-2

    Insert

    DHX36 (DHX36 Human)
    

    Use

    Tags

    Histidine

    Expression

    Bacterial, Insect, and Mamm…

    Mutation

    Promoter

    CMV ie enhancer/promoter

    Available since

    Sept. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

25. pLJC5-LAMP1-RFP-2xFlag

    Plasmid

    #102931

    Purpose

    Express a lysosomal Tag

    Depositor

    David Sabatini

    Article

    Abu-Remaileh et al Science. 2017 Nov 10;358(6364):807-813.

    Insert

    LAMP1
    

    Use

    Lentiviral

    Tags

    mRFP-2xFlag

    Expression

    Mutation

    Promoter

    ubc

    Available since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pTRKH2

    Plasmid

    #71312

    Purpose

    Theta replicating shuttle vector for E. coli and gram positive bacteria. High copy number in gram positives. Erythromycin resistance, recommended to use BHI agar plates for E. coli.

    Depositor

    Rodolphe Barrangou, Todd Klaenhammer

    Article

    O'Sullivan et al Gene. 1993 Dec 31;137(2):227-31.

    Type

    Empty backbone

    Use

    E. coli-gram positive bacteria shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TetO-FUW-oct4

    Plasmid

    #20323

    Depositor

    Rudolf Jaenisch

    Article

    Brambrink et al Cell Stem Cell. 2008 Feb 7. 2(2):151-9.

    Insert

    oct4 (Pou5f1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pYS19

    Plasmid

    #202084

    Purpose

    Frame -2 selector for NHEJ knock-in

    Depositor

    Daniel Dickinson

    Article

    Shi et al Development. 2023 May 15;150(10):dev2013

    Insert

    pEF1a-mCherry-Cas9 + Frame –2 sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    July 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV_CreNLS_PP7

    Plasmid

    #232439

    Purpose

    expression plasmid to produce Cre-NLS mRNA with a PP7 aptamer in the 3' UTR

    Depositor

    Gil Westmeyer

    Article

    Geilenkeuser et al Cell. 2025 May 15;188(10):2637-2655.e31.

    Insert

    Cre–MYC-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA3_2FLAG-AKAP79 WT

    Plasmid

    #183450

    Purpose

    Expression of human wild-type AKAP79

    Depositor

    Matthew Gold

    Article

    Patel et al Nat Commun. 2017 Nov 22;8(1):1681. doi:

    Insert

    AKAP5 (AKAP5 Human)
    

    Use

    Tags

    2xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge