169,843 results

Showing: 8791 - 8820 of 169843 results

Results per page:

Sort results by:

GIPR-DuET

Plasmid

#213252

Purpose

The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

Depositor

Thomas Sakmar

Article

Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

Insert

GIPR (GIPR Human)

Tags

1D4 and FLAG

Expression

Mammalian

Promoter

CMV, T7

Available Since

April 23, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-hSTING (R232)

Plasmid

#214152

Purpose

Transient expression of STING R232

Depositor

Nan Yan

Article

Dobbs et al Cell Host Microbe. 2015 Aug 12;18(2):157

Insert

STING (STING1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
HDAC6 Flag

Plasmid

#13823

Purpose

Mammalian expression of human histone deacetylase 6 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC6 (HDAC6 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

Enlarge
AmCyan-P2A-mCherry

Plasmid

#45350

Depositor

Ilpo Huhtaniemi

Article

Potorac et al J Endocrinol. 2016 Sep 21. pii: JOE-16-0

Insert

AmCyan

Tags

mCherry

Expression

Mammalian

Mutation

SV40 NLS

Promoter

CMV

Available Since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR/D_creERt2

Plasmid

#27321

Depositor

Leonard Zon

Article

Mosimann et al Development. 2011 Jan . 138(1):169-77.

Insert

creERt2

Use

Multisite gateway middle entry vector

Available Since

Jan. 14, 2011

Availability

Academic Institutions and Nonprofits only

Enlarge
Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry-C1 mCherry-NLS

Plasmid

#58476

Purpose

Expresses a nuclear-targeted mCherry control construct, on a CMV reporter

Depositor

Dyche Mullins

Article

Belin et al Elife. 2015 Aug 19;4. doi: 10.7554/eLife

Insert

3X NLS with 3X stop codons

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1(+)_4x(U6 tRNA M15)_CMV NESPylRS(AF)

Plasmid

#182652

Purpose

Encodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & 4x M15 tRNACUA used for amber codon suppression in mammalian cells

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Inserts

codon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
4xU6-tRNAM15

Tags

nuclear export signal (NES)

Expression

Mammalian

Mutation

Y306A/Y384F (AF) double mutant of Methanosarcina …

Promoter

CMV and U6

Available Since

May 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMpGWB104

Plasmid

#68558

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

GUS

Expression

Plant

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.2-Cx43-HA

Plasmid

#49851

Purpose

Encodes human connexin 43 with a C-terminal HA epitope fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

Connexin 43 (GJA1 Human)

Tags

HA

Expression

Mammalian

Mutation

Contains several wobble mutations to confer resis…

Promoter

CMV

Available Since

Jan. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pSpCas9(BB)-2A-Puro (PX459)

Plasmid

#48139

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62988.

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pDA056

Plasmid

#131387

Purpose

ORFeus expression, WT

Depositor

Kathleen Burns

Article

Ardeljan et al Nat Struct Mol Biol. 2020 Feb;27(2):168-

Insert

ORFeus-Hs

Expression

Mammalian

Promoter

TRE3G

Available Since

June 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBT281

Plasmid

#122611

Purpose

express evoAPOBEC1-BE4max in mammalian cells

Depositor

David Liu

Article

Thuronyi et al Nat Biotechnol. 2019 Jul 22. pii: 10.103

Insert

evoAPOBEC1-BE4max

Expression

Mammalian

Mutation

E4K H109N H122L D124N R154H A165S P201S F205S

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pDONR223-PRKAB1

Plasmid

#23360

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

PRKAB1 (PRKAB1 Human)

Use

Gateway donor vector

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDonor_ShCAST_kanR

Plasmid

#127924

Purpose

Kanamycin resistance donor for ShCAST

Depositor

Feng Zhang

Article

Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

Insert

KanR, R6K origin of replication

Available Since

Aug. 7, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
tet-TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Plasmid

#221235

Purpose

Tet-inducible lentiviral HACE Editor as a fusion of TadA-8e adenosine deaminase and an optimized PcrA M6 helicase.

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Insert

TadA8e-PcrA M6-UGI-PGK-Puro-T2A-rtTA

Use

Lentiviral

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pDestTol2-ubi-ASAP1

Plasmid

#121987

Purpose

ubiqutously express ASAP1 voltage biosensor

Depositor

GuangJun Zhang

Article

Silic et al J Vis Exp. 2018 Apr 25;(134). doi: 10.37

Insert

ASAP1

Use

Tol2 transposon destination vector

Promoter

zebrafish ubiquitin promoter

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMLS7-egfp

Plasmid

#112917

Purpose

Fluorescence microscopy

Depositor

Miguel Valvano

Article

Lefebre et al Appl Environ Microbiol. 2002 Dec;68(12):

Insert

eGFP

Expression

Bacterial

Promoter

PS7 promoter and Ps7

Available Since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pOpen-T5gene122

Plasmid

#165544

Purpose

Possesses two enzymatic activities: DNA synthesis (polymerase) and exonucleolytic activity that degrades ssDNA in the 3'-5' direction for proofreading purposes.

Depositor

Drew Endy, Jennifer Molloy, FreeGenes Project

Insert

T5 DNA Polymerase

Use

Synthetic Biology

Available Since

Aug. 9, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
CMV-loxp-DsRed-loxp-eGFP

Plasmid

#141148

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

loxp-DsRed-loxp-eGFP

Use

Lentiviral

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-hsyn-GRAB_DA2m

Plasmid

#140553

Purpose

Expresses the 2nd generation medium-affinity DA sensor GRAB_DA2m in neurons

Depositor

Yulong Li

Article

Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

Has Service

AAV9

Insert

The 2nd generation DA sensor GRAB_DA2m (medium affinity)

Use

AAV

Promoter

hSyn

Available Since

July 29, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta-arrestin2 GFP WT

Plasmid

#35411

Depositor

Robert Lefkowitz

Article

Shenoy et al J Biol Chem. 2005 Apr 15;280(15):15315-2

Insert

βarrestin2 (Arrb2 Rat)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-Nuc-ExRai-AktAR2

Plasmid

#184049

Purpose

Nuclear-targeted ExRai-AktAR2.

Depositor

Jin Zhang

Article

Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202

Insert

Nuc-ExRai-AktAR2

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCrlM

Plasmid

#122444

Purpose

Expresses myc tagged mouse reelin ORF in mammalian cells

Depositor

Tom Curran

Article

D'Arcangelo et al J Neurosci. 1997 Jan 1;17(1):23-31.

Insert

reelin (Reln Mouse)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTol1-UAS:ChrimsonR-tdTomato

Plasmid

#124231

Purpose

Zebrafish Tol1 construct for UAS-mediated expression of the optogenetic opsin ChrimsonR fused to the red fluorescent protein tdTomato

Depositor

Isaac Bianco

Article

Antinucci et al Elife. 2020 Mar 27;9. pii: 54937. doi: 1

Insert

ChrimsonR-tdTomato

Use

Zebrafish expression

Tags

tdTomato

Promoter

14xUAS-E1b

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pSAM2_mCherry_Gata4

Plasmid

#72690

Purpose

Doxycyline regulated expression of Gata4 in mammalian cells

Depositor

Timothy Kamp

Article

Lalit et al Cell Stem Cell. 2016 Mar 3;18(3):354-67.

Insert

Gata 4 (Gata4 Mouse)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV-Doxycycline

Available Since

Aug. 28, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-N-HA-NEK7

Plasmid

#75142

Purpose

Expresses N-terminal HA-tagged NEK7 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

May 20, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
hMEF2A WT

Plasmid

#118354

Purpose

This plasmid expresses human MEF2A-1 WT.

Depositor

Lea Sistonen

Article

Hietakangas et al Proc Natl Acad Sci U S A. 2006 Jan 3;103

Insert

hMEF2A (MEF2A Human)

Tags

myc and 6xHIS

Expression

Mammalian

Mutation

WT

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
FUW-tetO-NFIA

Plasmid

#141403

Purpose

Inducible Expression of NFIA

Depositor

Lorenz Studer

Article

Tchieu et al Nat Biotechnol. 2019 Mar;37(3):267-275.

Insert

NFIA (NFIA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
His-GST-TEV-BRD4(42-459) BD1-BD2

Plasmid

#233887

Purpose

pGex for protein overexpression of human BRD4

Depositor

Alessio Ciulli

Article

Hsia et al Nature. 2024 Mar;627(8002):204-211. doi:

Insert

BRD4 (BRD4 Human)

Tags

His-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge