We narrowed to 34,404 results for: CaS

1. p2CT-His-MBP-Lbu_C2c2_K1080A

   Plasmid

   #91862

   Purpose

   Bacterial expression for Cas13a

   Depositor

   Jennifer Doudna

   Article

   East-Seletsky et al Mol Cell. 2017 May 4;66(3):373-383.e3. d

   Insert

   LbuCas13a (LbuC2c2) K1080A
   

   Use

   Tags

   His6-MBP-TEV site

   Expression

   Bacterial

   Mutation

   K1080A

   Promoter

   Available since

   June 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJK384.4

   Plasmid

   #155369

   Purpose

   dCas9 + PA4-mVenus

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pJK412

   Plasmid

   #155371

   Purpose

   dCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]

   Depositor

   Pamela Silver

   Article

   Kuo et al Nucleic Acids Res. 2020 Aug 20;48(14):81

   Inserts

   dCas9 (bacteria)
   PA4-mVenus
   dCas9 sgRNA oscillator v1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   D10A H840A (catalytically inactive)

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1203F-5’+3'dDR-mCh(22)-GFP-bGHpolyA

   Plasmid

   #221536

   Purpose

   EGFP reporter construct with double unprocessed direct repeat (35nt) at 5' and 3' end, 22nt mCherry-targeting spacer in between DRs

   Depositor

   Omar Akbari

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. psicheck_200_300_CA_repeat

   Plasmid

   #65880

   Purpose

   Luciferase assay vector, assessing regulation of 200-300 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 200-300 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

6. psicheck_300_400_CA_repeat

   Plasmid

   #65879

   Purpose

   Luciferase assay vector, assessing regulation of 300-400 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 300-400 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 2, 2015

   Availability

   Academic Institutions and Nonprofits only

7. psicheck_50_100_CA_repeat

   Plasmid

   #65882

   Purpose

   Luciferase assay vector, assessing regulation of 50-100 nucleotide CA repeats

   Depositor

   Thomas Tuschl

   Insert

   CA repeats 50-100 nucleotides in length
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 23, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pCFD4-U6:1_U6:3tandemgRNAs

   Plasmid

   #49411

   Purpose

   expression of two gRNAs from Drosophila U6:1 and U6:3

   Depositor

   Simon Bullock

   Article

   Port et al Proc Natl Acad Sci U S A. 2014 Jul 7. pi

   Inserts

   dU6-1:gRNA
   dU6-3:gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   dU6-1 and dU6-3

   Available since

   Jan. 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pFYF1320 EGFP Site#1

   Plasmid

   #47511

   Purpose

   human gRNA expression vector targeting EGFP

   Depositor

   Keith Joung

   Article

   Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

   Insert

   gRNA-EGFP site 1
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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10. pEn-Chimera

    Plasmid

    #61432

    Purpose

    Gateway-Entry vector containing AtU6-26 promoter and sgRNA backbone

    Depositor

    Holger Puchta

    Article

    Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

    Insert

    AtU6-26:sgRNA
    

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    AtU6-26

    Available since

    Sept. 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pTBL3478 PB-PEmax-mTagBFP2

    Plasmid

    #226664

    Purpose

    PiggyBac cargo vector encoding PEmax and mTagBFP2

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Inserts

    PEmax
    mTagBFP2
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG and UBC

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRCC-N

    Plasmid

    #81192

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Nourseothricin Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTBL3241 PB-AtoBpegRNA-mCherry

    Plasmid

    #226666

    Purpose

    PiggyBac cargo vector encoding A to B pegRNA marked with mCherry

    Depositor

    Chang Liu

    Article

    Loveless et al bioRxiv 2021.11.05.467507

    Insert

    AtoB pegRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKDsgRNA-NT

    Plasmid

    #89960

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible non-targeting gRNA containing BsmBI sites for cloning.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    empty gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBT128c

    Plasmid

    #138512

    Purpose

    P2

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    inteinC-dSpCas9
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. GFP-itis pSel

    Plasmid

    #195344

    Purpose

    Selection plasmid expressing non-fluorescent EGFP A111V

    Depositor

    Alexis Komor

    Article

    Vasquez et al CRISPR J. 2023 Apr 20. doi: 10.1089/cris

    Insert

    EGFP
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    A111V

    Promoter

    J23119

    Available since

    Feb. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSL0527 (pDonor)

    Plasmid

    #130634

    Purpose

    Encodes a mini-transposon derived from V. cholerae CAST system, with CmR cargo gene. Total transposon size = 977 bp.

    Depositor

    Samuel H. Sternberg

    Article

    Klompe et al Nature. 2019 Jul;571(7764):219-225. doi:

    Insert

    VchCAST donor DNA (Mini-Tn)
    

    Use

    CRISPR; Transposon

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDsgRNA-rssA

    Plasmid

    #89957

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting rssA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    rssA gRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pTet

    Available since

    June 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry

    Plasmid

    #41863

    Purpose

    This plasmid is used to reconstitute the complete Type II CRISPR system from S. pyogenes and contains the host factor RNaseIII and tracrRNA.

    Depositor

    Feng Zhang

    Article

    Cong et al Science. 2013 Jan 3.

    Insert

    U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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