We narrowed to 154,128 results for: ins;

Showing: 5861 - 5880 of 154128 results

Results per page:

Sort results by:

pVk7-33G1-BioLHis

Plasmid

#58949

Purpose

backbone for mouse recombinant antibody with added C-terminal biotinylation site, linker, 6His tag

Depositor

Gavin Wright

Article

Staudt et al Biochem Biophys Res Commun. 2015 Jan 2;4

Type

Empty backbone

Tags

6His tag and biotinylation peptide

Expression

Mammalian

Promoter

CMV

Available Since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
Nrxn3α-AS4+

Plasmid

#184333

Purpose

Expression of mouse Nrx3-alpha containing insertion at the alternative splice site 4 together with EGFP protein separated by 2A peptide. Contains insertions at alternatively splice sites 1,2,3 and 6.

Depositor

Peter Scheiffele

Article

Nguyen et al Elife. 2016 Dec 13;5. doi: 10.7554/eLife

Insert

Nrxn3-alpha (Nrxn3 Mouse)

Tags

HA-tag

Expression

Mammalian

Promoter

CMV

Available Since

June 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-MBP-Cdc42-Q61L

Plasmid

#214288

Purpose

Express constitutively active Cdc42 fused with MBP

Depositor

Cliff Stains

Article

He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

Insert

Constitutively active Cdc42 (CDC42 Human)

Tags

Maltose-binding protein (MBP)

Expression

Bacterial

Mutation

Cdc42-Q61L

Promoter

Tac

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV_GW_Dest

Plasmid

#86955

Purpose

Gateway destination transfer plasmid for making AAV. Contains a DIO cassette for cre-induction. Without cre, insert is backwards in reference to promoter.

Depositor

Joseph Dougherty

Article

Cammack et al Proc Natl Acad Sci U S A. 2020 May 5;117

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

April 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
ZIKV_Sensor_32B_LacZ

Plasmid

#75007

Purpose

Toehold switch sensor to detect Zika Virus

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_sensor_32B_LacZ

Tags

LacZ

Expression

Bacterial

Promoter

T7

Available Since

March 2, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-CAG-LMO1 (GLuc-ChR2-EYFP)

Plasmid

#72888

Purpose

fusion protein of Gaussia luciferase, Channelrhodopsin-2, and enhanced yellow fluorescent protein (luminopsin) for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al PLoS One. 2013;8(3):e59759. doi: 10.1371

Insert

Gaussia luciferase, Channelrhodopsin-2, EYFP

Expression

Bacterial and Mammalian

Promoter

CAG

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX ShcD-SH2

Plasmid

#46488

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcD (SHC4 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 512-623

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX SH2-B-SH2

Plasmid

#46479

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2-B (SH2B1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 515-639

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pRSET a MatryoshCaMP6s

Plasmid

#100019

Purpose

Bacterial expression of fluorescent reporter for calcium signaling, based off of GCaMP6s. Contains a stable reference Large Stokes Shift (LSS) mOrange nested within the reporting cpEGFP.

Depositor

Wolf Frommer

Article

Ast et al Nat Commun. 2017 Sep 5;8(1):431. doi: 10

Insert

MatryoshCaMP6s

Tags

6x HIS tag and Xpress_EK tag

Expression

Bacterial

Promoter

T7

Available Since

Oct. 3, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CaMKIIa-Mac 3.0-EYFP

Plasmid

#35515

Purpose

Lentiviral expression of CaMKIIa-driven Mac 3.0 for optical inhibition.

Depositor

Karl Deisseroth

Article

Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

Insert

eMac 3.0-EYFP

Use

Lentiviral

Tags

EYFP

Expression

Mammalian

Mutation

Trafficking signal, ER export

Promoter

CaMKIIa

Available Since

May 2, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV5 MEKK4alpha KD

Plasmid

#12188

Depositor

Gary Johnson

Article

Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

Insert

MEKK4 (Map3k4 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Kinase dead: K1361M

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only
pME-CreLite

Plasmid

#131782

Purpose

Middle entry vector (Tol2 kit) with bi-cistronic CreLite system components, PhyBΔCreC and PIF6CreN, linked by P2A.

Depositor

George Eisenhoffer

Article

Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi

Insert

PhyBCreC-P2A-PIF6CreN

Use

Cre/Lox; Gateway cloning

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
MSP2253

Plasmid

#70704

Purpose

Bacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2015 Nov 2. doi: 10.1038

Inserts

mammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)

Use

CRISPR

Tags

NLS-3xFLAG

Expression

Bacterial

Mutation

E782K, N968K and R1015H in SaCas9

Promoter

T7

Available Since

Dec. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCellulose

Plasmid

#36300

Depositor

Jay Keasling

Article

Bokinsky et al Proc Natl Acad Sci U S A. 2011 Dec 13;10

Insert

pCellulose

Use

Synthetic Biology

Promoter

Two promoters from the E coli genome drive two di…

Available Since

Nov. 28, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
GEV 1

Plasmid

#12615

Depositor

John Louis

Article

Huth et al Protein Sci. 1997 Nov . 6(11):2359-64.

Insert

GB1 domain of protein G as N terminal tag, with Bcl2 as insert (Bcl2 Mouse)

Tags

G protein and His

Expression

Bacterial

Available Since

Sept. 22, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
ZIKV_Trigger_27B

Plasmid

#75008

Purpose

Portion of Zika Virus genome (KU312312: 1348-1494) containing sensor 27B trigger sequence

Depositor

James Collins, Alexander Green

Article

Pardee et al Cell. 2016 May 6. pii: S0092-8674(16)305

Insert

ZIKV_Trigger_27B

Expression

Bacterial

Promoter

T7

Available Since

Feb. 4, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pMS1: pHelper(AvCAST)_AvPSP1

Plasmid

#168133

Purpose

Inducible expression of AvCAST proteins with crRNA targeting AvPSP1.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Inserts

AvCAST minimal CRISPR array (with spacer for AvPSP1)
AvCAST Cascade proteins (Cas6, Cas8, Cas7 and Cas5)
AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)
AvTnsD

Expression

Bacterial

Available Since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
HOXA10-MSCV

Plasmid

#20980

Depositor

Corey Largman

Article

Thorsteinsdottir et al Mol Cell Biol. 1997 Jan . 17(1):495-505.

Insert

HOXA10 (HOXA10 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pHBS952 TARDBP-Exon1-ko-sgRNA1-SpCas9

Plasmid

#107857

Purpose

To knock-out TDP43

Depositor

Rajat Rohatgi

Article

Schmidt et al Nat Commun. 2019 Oct 25;10(1):4890. doi:

Insert

gRNA against TARDBP (Exon 1) (TARDBP Human)

Expression

Mammalian

Available Since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pET28a(+) 6xHis-TEV-IFIT3

Plasmid

#53559

Purpose

bacterial expression of IFIT3

Depositor

Kathleen Collins

Article

Katibah et al Mol Cell. 2013 Feb 21;49(4):743-50. doi:

Insert

IFIT3 (IFIT3 Human)

Tags

6xHIS and TEV

Expression

Bacterial

Promoter

T7

Available Since

May 29, 2014

Availability

Academic Institutions and Nonprofits only