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pLenti-hU6-mU6-BlasGO

Plasmid

#136906

Purpose

Lentivirus for base editing activatable Blasticidin resistance gene expression in mammalian cells. All in one vector with sgBlasGO.

Depositor

Lukas Dow

Article

Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

Insert

BlasCyGO

Use

Lentiviral

Mutation

ATG>ACG

Promoter

SFFV

Available Since

Feb. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET15b/BOA

Plasmid

#82041

Purpose

For E.coli expression of 6xHis-Tagged BOA (Burkholderia oklahomensis agglutinin)

Depositor

Angela Gronenborn

Article

Whitley et al FEBS J. 2013 May;280(9):2056-67. doi: 10

Insert

Burkholderia oklahomensis agglutinin

Tags

6x His

Expression

Bacterial

Available Since

June 21, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV2_VP1_RaPID_r1c3

Plasmid

#65723

Purpose

Produce random mCherry insertion in AAV2 VP3 region of VP1; ITRs in VP1 only

Depositor

Junghae Suh

Article

Judd et al Mol Ther Nucleic Acids. 2012 Nov 13;1:e5

Insert

mCherry

Use

AAV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA5 FRT/TO Plk4 KD-mCherry

Plasmid

#80270

Purpose

mammalian expression plasmid for kinase dead Plk4

Depositor

Andrew Holland

Article

Moyer et al J Cell Biol. 2015 Jun 22;209(6):863-78.

Insert

Plk4 (PLK4 Human)

Tags

mCherry

Expression

Mammalian

Mutation

D154A kinase dead

Promoter

CMV

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pEBG-TFII-I-p70

Plasmid

#22187

Depositor

Ananda Roy

Article

Cheriyath et al Mol Cell Biol. 1998 Aug . 18(8):4444-54.

Insert

TFII-I (GTF2I Human)

Tags

GST and His

Expression

Mammalian

Mutation

Contains only amino acids 1-735 C-terminal residu…

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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pGL4-Gateway-SCP1

Plasmid

#71510

Purpose

humanSTARR-seq validation vector with Gateway cassette upstream of the firefly luciferase gene and the SCP1 core promoter

Depositor

Alexander Stark

Article

Arnold et al Science. 2013 Mar 1;339(6123):1074-7. do

Type

Empty backbone

Use

Humanstarr-seq validation vector

Tags

luciferase

Expression

Mammalian

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only
IBMc460

Plasmid

#161940

Purpose

Carries Golden Gate substitution insert for IBM with gp41-1 intein. PPhlF as C-lobe promoter

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pSB1A3-BbsI-GY-gp-41-1N-cat-PPhlF-RiboJ-B32-gp41-1C-SS-SapI

Expression

Bacterial

Promoter

PPhlF

Available Since

March 30, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Tag2B Flag BimS

Plasmid

#23283

Depositor

Roger Davis

Article

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Insert

pCMV Tag2b Bim S (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pCAGGS-PB1-AG(Y63L)-LOV2

Plasmid

#178876

Purpose

Mammalian expression of PB1-AzamiGreen(Y63L)-LOV2

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen(Y63L)-LOV2

Expression

Mammalian

Mutation

AzamiGreen: Y63L

Promoter

CAG

Available Since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-PB1-AG-FRB-TEVcs-mTagBFP2-HA

Plasmid

#178868

Purpose

Mammalian expression of a protein of interest fused to the C-terminus of PB1-AzamiGreen-FRB-TEVcs-mTagBFP-HA

Depositor

Shinya Tsukiji

Article

Yoshikawa et al J Am Chem Soc. 2021 May 5;143(17):6434-6

Insert

PB1-AzamiGreen-FRB-TEVcs(ENLYFQL)-mTagBFP2-HA

Expression

Mammalian

Promoter

CMV

Available Since

March 3, 2022

Availability

Academic Institutions and Nonprofits only

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attB53-Pac-attB53

Plasmid

#183610

Purpose

RMCE vector to shuttle puromycin resistance into attP50-flanked landing pad. Designed for use with pmROSA26-attP50-Neo-mKate2-3xNLS-attP50 vector (Addgene 183609).

Depositor

Sally Lowell

Article

Malaguti et al Development. 2022 May 26. pii: 275525. d

Insert

Pac

Use

Rmce

Available Since

June 3, 2022

Availability

Academic Institutions and Nonprofits only

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pMCSG7-D16A-NmeCas9

Plasmid

#71476

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with a D16A mutation (RuvC domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-D16A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

D16A

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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cacnb1b (rat) in pMT2 vector

Plasmid

#107423

Purpose

Expresses Calcium channel beta1b auxillary subunit in mammalian cells.

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 1997 Feb 15;17(4):1330-8.

Insert

calcium channel beta1b auxillary subunit

Expression

Mammalian

Promoter

Ad MLP

Available Since

April 12, 2018

Availability

Academic Institutions and Nonprofits only

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pMCSG7-H588A-NmeCas9

Plasmid

#71477

Purpose

bacterial pMCSG7 expression vector expressing Nme cas9 nickase with H588A mutation (HNH domain) with T7 promoter, N-terminal His tag and TEV site

Depositor

Erik Sontheimer

Article

Zhang et al Mol Cell. 2015 Oct 15;60(2):242-55. doi:

Insert

pMCSG7-H588A-NmeCas9

Tags

6His

Expression

Bacterial

Mutation

H588A

Promoter

T7

Available Since

Feb. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pLV CDHblast Rev-EGFP-V5

Plasmid

#175164

Purpose

Lentiviral expression of Rev-EGFP-V5

Depositor

Larry Gerace

Article

Hammond et al J Mol Biol. 2018 Feb 16;430(4):537-553.

Insert

rev

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Jan. 20, 2022

Availability

Academic Institutions and Nonprofits only

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GFAP-Archon-EGFP

Plasmid

#187979

Purpose

Expresses the fluorescent voltage indicator Archon under the GFAP promoter

Depositor

Chris Dulla

Article

Armbruster et al Nat Neurosci. 2022 May;25(5):607-616. do

Insert

Archon1-EGFP

Use

AAV

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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H2A-mTurquoise-RHOG-QL-deltaCAAX

Plasmid

#176096

Purpose

Constitutively active, nuclear localized RhoG

Depositor

Dorus Gadella

Article

Mahlandt et al J Cell Sci. 2023 May 15;136(10):jcs26080

Insert

RHOG (RHOG Human)

Expression

Mammalian

Mutation

QL

Available Since

Oct. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pT7-th-sgRNA

Plasmid

#65565

Purpose

in vitro trancription of sgRNA targeting the zebrafish th locus

Depositor

Jiu-lin Du

Article

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

th sgRNA (th Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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AL13Rb-GAPC2

Plasmid

#161009

Purpose

PP7-tagged reporter driven by the Arabidopsis GAPC2 promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Expression

Plant

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-mIFITM1

Plasmid

#58415

Purpose

Expresses murine IFITM1 with an N-terminal HA tag in mammalian cells.

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Tags

HA

Expression

Mammalian

Promoter

CMV IE

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only