We narrowed to 13,159 results for: OVA

1. pGEMHE:mTREK-1(S131C/A286F)

   Plasmid

   #224748

   Purpose

   In vitro transcription for Oocyte expression of mTREK-1(S131C/A286F)

   Depositor

   Dan Minor

   Article

   Deal et al Cell Chem Biol. 2024 Jul 18;31(7):1305-1

   Insert

   Potassium channel subfamily K member 2 (Kcnk2 Mouse)
   

   Use

   In vitro transcription for oocyte expression

   Tags

   Expression

   Mutation

   S131C / A286F

   Promoter

   T7

   Available since

   Sept. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMT-C34-H

   Plasmid

   #223966

   Purpose

   Anti-MT-C34 heavy chain expression plasmid; to be used with the light chain plasmid, pMT-C34-L (Plasmid 223967), to make the antibody.

   Depositor

   Dmitriy Mazurov

   Article

   Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

   Insert

   Variable domain of mouse anti-MT-C34 IgG1 heavy chain jointed to constant region of human IgG1 heavy chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMT-C34-L

   Plasmid

   #223967

   Purpose

   Anti-MT-C34 light chain expression plasmid; to be used with the heavy chain plasmid, pMT-C34-L (Plasmid 223966), to make the antibody.

   Depositor

   Dmitriy Mazurov

   Article

   Kalinichenko et al Biology (Basel). 2024 Aug 29;13(9):675.

   Insert

   Variable domain of mouse anti-MT-C34 IgG1 light chain jointed to constant region of human IgG1 light chain
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubc-c005

   Plasmid

   #216876

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. Ubc-c004

   Plasmid

   #216875

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-DONSON-DEWS

   Plasmid

   #208799

   Purpose

   Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A, S437A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   D374A; E377A; W381A; S437A

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-DONSON-3A

   Plasmid

   #208800

   Purpose

   Xenopus laevis His6-Myc-DONSON (V473A, R476A, Y481A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   V473A; R476A; Y481A

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-DONSON-Δ24

   Plasmid

   #208801

   Purpose

   Xenopus laevis His6-Myc-DONSON (1-24aa removed)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   Δ24, deletion of 1-24aa

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-DONSON-DEW

   Plasmid

   #208802

   Purpose

   Xenopus laevis His6-Myc-DONSON (D374A, E377A, W381A)

   Depositor

   Aga Gambus

   Article

   Cvetkovic et al Mol Cell. 2023 Nov 16;83(22):4017-4031.e

   Insert

   DONSON (donson.L Frog)
   

   Use

   Tags

   His and Myc

   Expression

   Bacterial

   Mutation

   D374A; E377A; W381A

   Promoter

   T7

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pEJS1983 Lenti-dead_GFP_ABE reporter-BlastR

    Plasmid

    #211820

    Purpose

    Lentiviral vector expressing a dead GFP adenine base editing reporter

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    dead GFP reporter
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter and SV40 promoter

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS-HZ08-pAAVsc.U6-tracrRNA

    Plasmid

    #211816

    Purpose

    AAV vector expressing tracrRNA under U6 promoter

    Depositor

    Erik Sontheimer

    Article

    Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

    Insert

    U6-tracrRNA
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Qt-FLAG-TGA-rt9us-antiGFP

    Plasmid

    #206954

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~8% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLINE1mORF1-ΔORF2-scFvFc

    Plasmid

    #206023

    Purpose

    The base next to the ORF1 start codon (ATG) was deleted in pLINE1ΔORF2-scFvFc

    Depositor

    Masamichi Kamihira

    Article

    Zheng et al Biotechnol J. 2021 Jul;16(7):e2000620. d

    Insert

    mouse LINE1 vector harboring an scFv-Fc expression unit, encoding mutated ORF1 but lacking ORF2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pETDUET-GST AO7RE ∆238-245

    Plasmid

    #139318

    Purpose

    GST tagged AO7 encoding aa 126-258 with an internal deletion that results in loss of E2 binding for expression in bacteria

    Depositor

    Allan Weissman

    Article

    Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

    Insert

    AO7 aa 126-258 (RNF25 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    AO7 cloned into the first multiple cloning site o…

    Promoter

    T7 promoter-1

    Available since

    Dec. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGEMHE-MmCENP-O-GFP

    Plasmid

    #205178

    Purpose

    Expresses mouse CENP-O C-term tagged with GFP under T7 promoter - designed for in vitro transcription

    Depositor

    Michael Lampson

    Article

    Dudka et al J Cell Biol. 2023 Sep 4;222(9):e20221208

    Insert

    CENP-O (Cenpo Mouse)
    

    Use

    In vitro transcription

    Tags

    5 glycines linker followed by GFP

    Expression

    Mammalian

    Mutation

    Wild type mouse CENP-O

    Promoter

    T7

    Available since

    Oct. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. Qt-FLAG-TAA-rt20s-antiGFP

    Plasmid

    #206955

    Purpose

    Mammalian expression of Qt EMcapsulins targeting GFP with stoichiometry control (~6% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. 1M-Qt-FLAG-TGA-rt20s-antiGFP

    Plasmid

    #206953

    Purpose

    Mammalian expression of 1M-Qt EMcapsulins targeting GFP with stoichiometry control (~20% surface decoration)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    MmMT3, QtEnc, antiGFP-intrabody
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcS-RDG-C1C2

    Plasmid

    #200163

    Purpose

    This plasmid encodes non-binder control monobody (RDG) fused to the extracellular vesicle binding domain (C1C2) of lactadherin for surface engineering extracellular vesicles.

    Depositor

    Masako Harada

    Article

    Komuro et al Bioengineering (Basel). 2022 Jan 29;9(2)

    Inserts

    RDG-C1C2
    C1C2 domain of lactadherin
    

    Use

    Tags

    HA and HIS

    Expression

    Mammalian

    Mutation

    sequence adopted from doi: 10.1371/journal.pone.0…

    Promoter

    Available since

    Sept. 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGL4.23-Gja1prom

    Plasmid

    #188114

    Purpose

    Expresses luciferase under control of Gja1 promoter in transfected cells

    Depositor

    Jennifer Mitchell

    Article

    Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

    Insert

    Gja1 promoter (Gja1 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Gja1 endogenous promoter up to gene start codon

    Available since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENeCFPCNAL2 pc922

    Plasmid

    #167563

    Purpose

    For construction of CFP-tagged human PCNA, the GFP coding sequence in the pENeGFPCNAL2 vector was replaced by the ECFP coding sequence from the pECFP-C1 vector (Clontech Laboratories, Inc., CA).

    Depositor

    Cristina Cardoso

    Article

    Zhang et al Nucleic Acids Res. 2017 Apr 25. doi: 10.

    Inserts

    Proliferating Cell Nuclear Antigen (PCNA Human)
    CFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pET-28a(+)-6xHis-TEV-ORF9c

    Plasmid

    #201023

    Purpose

    Bacterial expression of codon optimized SARS-CoV-2 ORF9c tagged with an N-terminus His tag followed by a TEV cleavage site.

    Depositor

    Sharon Rozovsky

    Article

    Ghelichkhani et al J Mol Biol. 2023 Apr 15;435(8):168008. d

    Insert

    open reading frame 9c
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Gene insert is codon optimized for Ecoli.

    Promoter

    T7/LacO

    Available since

    June 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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22. DNMT2Δ191-237

    Plasmid

    #198382

    Purpose

    Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

    Depositor

    Tanja Schirmeister

    Article

    Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

    Insert

    DNMT2 (TRDMT1 Human)
    

    Use

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    deleted amino acids 191-237

    Promoter

    T7

    Available since

    May 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pX330A-Yap1-E1-#2

    Plasmid

    #171519

    Purpose

    deletion of a genomic locus in Yap1 gene

    Depositor

    Hans Schöler

    Article

    Kagiwada et al Development. 2021 Oct 15;148(20). pii: 2

    Insert

    Yap1 (Yap1 Mouse)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX4T3-GST-PolB(T304I)

    Plasmid

    #177133

    Purpose

    Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I)

    Depositor

    Robert Sobol

    Article

    Fang et al Nucleic Acids Res. 2019 Jul 9;47(12):626

    Insert

    PolB (POLB Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Sept. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. GLE_sgRNA

    Plasmid

    #186658

    Purpose

    GLE sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Gle sgRNA Plasmid (CG14749 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. piwi_sgRNA

    Plasmid

    #186653

    Purpose

    piwi sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Piwi sgRNA Plasmid (piwi Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pIG-742pUC19-tNGFR-P2A-Caspase8

    Plasmid

    #186099

    Purpose

    Knockin of truncated NGFR to target gene

    Depositor

    Alexander Marson

    Article

    Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

    Insert

    CASPASE8
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    WT with tNGFR sequence

    Promoter

    Available since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. Nbr_C_sgRNA2

    Plasmid

    #186664

    Purpose

    Nbr C-tag sgRNA2 plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Nbr sgRNA 2 Plasmid (Nbr Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Nbr_C_sgRNA1

    Plasmid

    #186663

    Purpose

    Nbr C-tag sgRNA1 plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Nbr sgRNA 1 Plasmid (Nbr Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Shu_sgRNA

    Plasmid

    #186656

    Purpose

    shu sgRNA plasmid

    Depositor

    Astrid Haase

    Article

    Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

    Insert

    Shu sgRNA Plasmid (shu Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge